• Korean Journal of Organic Agriculture
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• v.27 no.2
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• pp.147-160
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• 2019

This study was conducted to evaluate roughage to concentrate ratio on the changes of productivity and metabolic profiling in milk. Six lactating Holstein cows were divided into two groups, T1 group was fed low-concentrate diet (Italian ryegrass to concentrate ratio = 8:2) and T2 group was fed high-concentrate diet (Italian ryegrass to concentrate ratio = 2:8). Milk samples were collected and its components and metabolites were analyzed by ¹H-NMR (Nuclear magnetic resonance). The result of milk components such as milk fat, milk protein, solids-not-fat, lactose and somatic cell count were not significantly different between two groups. In carbohydrate metabolites, trehalose and xylose were significantly higher (P<0.05) in T1 group, however lactose was not significantly different between two groups. In amino acid metabolites, glycine was the highest concentration however, there was no significant difference observed between two groups. Urea and methionine were significantly higher (P<0.05) in the T2 group. In lipid metabolites, carnitine, choline and O-acetylcarnitine there were no significant difference observed between the two groups. In benzoic acid metabolites, tartrate was significantly higher (P<0.05) in T2 group. In organic acid metabolites, acetate was significantly higher (P<0.05) in T1 group and fumarate was significantly higher (P<0.05) in T2 group. In the other metabolites, 3-methylxanthine was only significantly higher (P<0.05) in T2 group and riboflavin was only significantly higher (P<0.05) in T1 group. As a result, milk components were not significantly different between two groups. However, metabolites concentration in the milk was significantly different depends on roughage to concentrate ratio.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.12
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• pp.814-824
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• 2018

This study was conducted to investigate VFA, monosaccharides and metabolites in rumen fluid according to feeding methods. Three castrated Hanwoo steers were used to the $3{\times}3$ Latin square design, 10 day for the diet adaptation period. VFA and monosaccharides which were not detected by HPLC and HPAEC however, those were detected by $^1H-NMR$. Among the metabolites measured by $^1H-NMR$ carbohydrate metabolites, pyruvate was detected only in the rumen fluid before feeding and succinate was detected before and after feeding rumen fluid. In amino acid total 9 metabolites were detected. In lipid metabolites, ethylene glycol was significantly higher (P<0.05) in before feeding Con group. In aliphatic acylic metabolite, trimethylamine N-oxide was no significant difference observed compare to Con group. In this study, many metabolites were observed in the rumen fluid by $^1H-NMR$, and it confirmed that rumen metabolic products were changed by feeding methods.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.264-268
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• 1997

Microbial metabolites from rhizosphere soil samples mainly inhabitated by Streptomyces are selectively uptaken into plants. The culture broth of a Streptomyces strain K9301 showed a major metabolite which disappeared in the medium 24hrs after planting of seedlings. This metabolite was selectively uptaken in the rice plants as well as the wheat plants. We identified the targeted metabolite showing a strong UV-absorbing spot at Rf 0.6 on TLC to be 2-aminobenzamide.

• Feed Journal
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• v.2 no.10
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• pp.116-119
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• 2004

사료중에 존재하는 위해요소로서, 곰팡이 독소는 아스퍼질러스, 페니실리움, 푸사리움속에 속하는 곰팡이의 2차 대사산물이며, 사람과 가축에 잠재적 독성이 있는 300여 가지의 곰팡이 대사산물이 발견되어지고 있다.(중략)

• Korean Journal of Environmental Agriculture
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• v.21 no.1
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• pp.57-68
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• 2002

In order to elucidate the effect of phenobarbital sodium (PB) and 3-methylcholanthrene (3-MC) on metabolism of insecticide carbofuran in rat. Carbofuran metabolites and its formation rates were determined when orally administered $^{14}C$-carbofuran alone and its combination with PB or 3-MC to rat. $^{14}C$-carbofuran administered orally, alone or in combination with PB or 3-MC, was secreted rapidly within 48 hrs. That is, 79.9 to 81.1% of the original radioactivity was secreted into the urine and 5.7 to 6.5% into the feces. The secretion rate was faster in the combined administration than that in carbofuran alone. Metabolites of carbofuran in main organs, urine, feces and blood of rat were largely 3-hydroxycarbofuran, 3-ketocarbofuran, 3-hydroxycarbofuran phenol, 3-ketocarbofuran phenol, and carbofuran phenol, the major ones being 3-hydroxycarbofuran and 3-ketocarbofuran, respectively, in all administrations of carbofuran alone, carbofuran+PB and carbofuran+3-MC. In addition, formation rate of the two major metabolites detected in the urine was 17.4% and 12.8%, respectively, when carbofuran alone was administered. Meanwhile, when carbofuran was administered with PB or 3-MC, they were 8.6% and 23.5, repectively. These results indicate that the oral administration of PB or 3-MC can reduce carbofuran toxicity by fastening and stimulating the carbofuran metabolism in rat.

• Applied Biological Chemistry
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• v.38 no.5
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• pp.463-467
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• 1995

In vitro metabolism study of ${\alpha}$-endosulfan by liver and kidney microsomal cytochrome P-450 monooxygenase system of the mouse(Balb/C) was performed. ${\alpha}$-Endosulfan was metabolized to endosulfan lactone(EL), endosulfan hydroxyether(EHE), endosulfan alcohol(EA), endosulfan sulfate(ES), endosulfan ether(EE) and ${\beta}$-endosulfan(${\beta}$-E). The main metabolites of ${\alpha}$-endosulfan were EL(13.2%) and EA(11.5%) in liver microsome and EA(17.4%) md EHE(19.3%) in kidney microsome. The $^{14}C$-activity of organic extractable fraction and water soluble fraction were 63.4% and 31.7% in liver micosome incubates respectively. The water soluble metabolites were EA(83.9%), EHE(4.5%) and ES(2.3). Piperonyl butoxide treatment inhibited the formation of EE by 86%, EA by 92% and EHE, EL and ES were barely formed.

• KOGO NEWS
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• v.7 no.3
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• pp.3-10
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• 2007

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.153-153
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• 1993

Prostaglandin은 인체의 여러 조직에서 생성되어 체내에 널리 분포되어 있지만 그 함유량은 극히 적다. 또한 대사산물의 반감기가 매우 이에 대한 연구결과를 알기 위하여는 조직 또는 체액을 추출하여 세심한 주의가 필요하고 측정방법에 있어서 정밀성이 요구된다. 또 여러가지 대사산물과 전구물질을 분리할 수 있는 방법이 요구된다. Prostaglandin가 생성되고 대사되는 과정이 매우 복잡하기 때문에 보다 정확하게 여러가지 대사산물을 분리할 수 있는 방법이 요구된다. 한편, prostaglandin은 전신의 거의 모든 조직에 분포하며 그 이용도 다양하다. 뿐만 아니라, prostaglandin이 임상적으로 생체기능의 조절(고혈압, 신기능조절, 난소와 자궁의 생리 등) 및 prostaglandin analogue의 길항약물 및 생합성 억제약물로서도 중요한 의미를 가지고 있다. 본 연구에서는 이러한 관점에서 prostaglandin을 측정하는데 있어서: 1. 조직의 추출과 조작에 대한 일반적 방법 2. 생체조직에서 eicosanoid의 추출 방법 3. Prostaglandin, thromboxane 및 leukotriene에 대하여 평활근을 이용한 생물학적 검정 4. Eicosanoids의 방사면역학적 검정 5. 효소면역 검정법 6. Cyclooxygenase의 측정, 정체 및 특성 7. Lipoxygenase의 특성과 측정 8. 지질과산화 반응의 측정 등을 다루었다.

• Korean journal of applied entomology
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• v.30 no.1
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• pp.10-17
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• 1991

Cuticular penetration and detoxication as mechanisms of resistance to the carbamate insecticides in fenobucarb-selected($R_{f}$) and carbofuran-selected($R_{c}$) strains of the brown planthopper (N. lugens Stal) were investigated. Rates of penetration were not significantly different in the susceptible and resistant strains. However, total amount of excretion of the $R_{f}$ and $R_{c}$ strains were much larger than that of the susceptible strain. Fenovucarb and carbofuran were in vivo metabolizd much faster in the $R_{f}$ strain than in the susceptible strain. OSBP(o-sec-butyl phenol) and 3-ketocarbofuran phenol were invitro the major metabolites of fenobucarb and carbofuran in the brown planthopper, respectively. Total amount of the two major metabolites were produced abotu 2 times larger in the $R_{f}$ and $R_{c}$ strains compared to the susceptible strain. OSBP and 3-ketocarbofuran phenol were not so toxic to the brown planthopper ($LD_{50}$ >100 $\mu\textrm{g}$/g hopper). Based on our data, detoxication plays a large role in resistance to fenobucarb and carbofuran in the resistant strain of BPH, although several resistance factors maybe involved.

• Korean Journal of Environmental Agriculture
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• v.16 no.1
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• pp.25-30
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• 1997

Absorption, distribution, metabolism and excretion of $^{14}C-carbofuran$(2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamte) were studied in carp(Cyprinus carpio L.) after the treatment of carbofuran at the dose level of 43 parts per billion. Maximum radioactivities in tissues(liver, kidney, gut, gall bladder) and blood of carp were shown 12hrs after the treatment of $^{14}C-carbofuran$. Carbofuran was metabolized to 3-hydroxycarbofuran and 3-ketocarbofuran in liver and kindney of carp, and the major metabolite was 3-hydroxycarbofuran. Most radioactivity absorbed into the carp tissues was eliminated 3hrs after transfer of the carp to fresh water. The excretory metabolites were 3-ketocarbofuran(32.3%), 3-hydroxycarbofuran(52.8%) and an unknown metabolite(2.6%) during the period of 3hrs of the excretory experiment.