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Study on the Food Habits of Sika Deer(Servus Nippon) Fed with Various Roughage Sources (여러 가지 조사료를 급여한 꽃사슴(Servus nippon)의 채식습성에 관한 연구)

  • Lee, In-Duk;Lee, Hyung-Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.28 no.1
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    • pp.61-70
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    • 2008
  • The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

Flurprimidol, Paclobutrazol, and Trinexapac-ethyl Increased Lateral Development of 'Zenith' Zoysiagrass in a Shade Environment (그늘조건에서 Flurprimidol, paclobutrazol, and trinexapac-ethyl이 한국잔디 'Zenith'의 수평생장 증가에 미치는 영향)

  • Ryu, Ju-Hyun;Kim, Ki-Sun
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.149-155
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    • 2010
  • This study was conducted to examine the effects of plant growth regulators (PGRs) on the lateral stem growth, and the change of total nonstructural carbohydrate (TNC) status of 'Zenith' zoysiagrass under shaded conditions. Well-established turf was subjected to a full sun, 47%, and 77% shade in a field study. Three different rates of flurprimidol (FP: 0.2, 0.4, and $0.8\;kg{\cdot}ha^{-1}$), paclobutrazol (PB: 0.16, 0.32, and $0.64\;kg{\cdot}ha^{-1}$), and trinexapac-ethyl (TE: 0.04, 0.08, and $0.16\;kg{\cdot}ha^{-1}$) were applied. Lateral development of 'Zenith' zoysiagrass decreased with increasing shade levels. However, compared with control plots, total stolon length and stolon number increased two fold at 0.2, $0.4\;kg{\cdot}ha^{-1}$ FP, and $0.16\;kg{\cdot}ha^{-1}$ PB under full sun. Under 77% shading, stolon number increased by 170% and total stolon length increased by 140% at $0.8\;kg{\cdot}ha^{-1}$ FP. Tiller number increased by 40% at $0.08\;kg{\cdot}ha^{-1}$ TE under full sun, and by 72% at $0.16\;kg{\cdot}ha^{-1}$ TE under 77% shading. The TNC contents of turfgrass treated with $0.8\;kg{\cdot}ha^{-1}$ FP and $0.16\;kg{\cdot}ha^{-1}$ TE increased by 50% as compared with control. Remarkably, nonstructural carbohydrates (NC) partitioning was enhanced by PGRs from leaf tissue to lateral stem tissue, which increased lateral development and may have contributed to recuperative rate. These results suggested that treatments of proper rate of PGRs could enhance the recuperative rate of 'Zenith' zoysiagrass by increasing lateral stem growth especially in shaded conditions.

A Preliminary Analysis on the International Management System for the Ocean fertilization with Iron at High Seas (해양 철분 시비(施肥)사업의 국제 관리체제 예비 분석)

  • Hong, Gi-Hoon;Sohn, Hyo-Jin
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.11 no.3
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    • pp.138-149
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    • 2008
  • Rapid accumulation of carbon dioxide in the atmosphere for the past century leads to acidify the surface ocean and contributes to the global warming as it forms acid in the ocean and it is a green house gas. In order to curb the green house gas emissions, in particular carbon dioxide, various multilateral agreements and programs have been established including UN Convention of Climate Change and its Kyoto Protocol for the last decades. Also a number of geo-engineering projects to manipulate the radiation balance of the earth have been proposed both from the science and industrial community worldwide. One of them is ocean fertilization to sequester carbon dioxide from the atmosphere through the photosynthesis of phytoplankton in the sea. Deliberate fertilization of the ocean with iron or nitrogen to large areas of the ocean has been proposed by commercial sector recently. Unfortunately the environmental consequences of the large scale ocean iron fertilization are not known and the current scientific information is still not sufcient to predict. In 2007, the joint meeting of parties of the Convention on the Prevention of Marine Pollution by Dumping of Wastes and Other Matter, 1972 and 1996 Protocol (London Convention/Protocol) has started considering the purposes and circumstances of proposed large-scale ocean iron fertilization operations and examined whether these activities are compatible with the aims of the Convention and Protocol and explore the need, and the potential mechanisms for regulation of such operations. The aim of this paper is to review the current development on the commercial ocean fertilization activities and management regimes in the potential ocean fertilization activities in the territorial sea, exclusive economic zone, and high seas, respectively, and further to have a view on the emerging international management regime to be London Convention/Protocol in conjunction with a support from the United Nations General Assembly through The United Nations Open-ended Informal Consultative Process on Oceans and the Law of the Sea.

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Irrigation Control for Improving Irrigation Efficiency in Coir Substrate Hydroponic System (코이어 배지 수경재배에서 관수효율 향상을 위한 급액 제어)

  • Yoo, Hyung-Joo;Choi, Eun-Young;Lee, Yong-Beom
    • Journal of Bio-Environment Control
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    • v.24 no.3
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    • pp.153-160
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    • 2015
  • The objectives of this study were to determine optimal length of off-time between irrigation cycles to improve irrigation efficiency using a frequency domain reflectometry (FDR) sensor-automated irrigation (FAI) system for tomato (Solanum lycopersicum L.) cultivation aimed at minimizing effluent from coir substrate hydroponics. For treatments, the 5-minute off-time length between 3-minute run-times (defined as 3R5F), 10-minute off-time length between 3-minute run-times (defined as 3R10F), or 15-minute off-time length between 5-minute run-times (defined as 5R15F) were set. During the 3-minute or 5-minute run-time, a 60mL or 80mL of nutrient solution was irrigated to each plant, respectively. Until 62 days after transplant (DAT) during the autumn to winter cultivation, daily irrigation volume was in the order of 3R5F (858mL) > 5R15F (409mL) > 3R10F (306mL) treatment, and daily drainage ratio was in the order of 3R5F (44%) > 5R15F (23%) > 3R10F (14%). Between 63 and 102 DAT, daily irrigated volume was in the order of 5R15F (888mL) > 3R5F (695mL) > 3R10F (524mL) with the highest drainage ratio, 19% (${\pm}2.6$), at the 5R15F treatment. During the spring to summer cultivation, daily irrigation volume and drainage ratio per plant was higher in the 3R5F treatment than that of the 3R10F treatment. For both cultivations, a higher water use efficiency (WUE) was observed under the 3R10F treatment. Integrated all the data suggest that the optimal off-time length is 10 minutes.

Radurization Effect of Korean Mackerel (한국산 고등어의 방사선 조사효과)

  • CHO S. J.;CHOE Y. K.;LEE S. Y.;BYUN S. M.;CHUNG J. R.
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.3
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    • pp.219-226
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    • 1985
  • To investigate potential application of radurization technique as an effective tool for controlling histamine accumulation, histamine contents were determined in fresh Korean mackerel meat samples irradiated at six dose levels of gamma radiation up to 1 Mrad during 35 day-storage at $0^{\circ},\;5^{\circ}\;and\;10^{\circ}C$. Total bacterial counts, total volatile bases and trimethylamine contents were also estimated. The minimum and maximum doses for Korean mackerel were determined to be 0.2 and 0.5 Mrad, respectively. Irradiation induced redening of fish muscle tissue and redening appeared to be dose dependent. At low doses, this redening contributed positively to the overall sensory qualities. At 0.5 and 1.0 Mrad, on the other hand, meat samples were excessively redened to the extent that red and white muscle layers were not readily distinguishable. No appreciable histamine accumulation occured during entire 35 day-storage in fresh mackerel meats, whether irradiated or not, when they were kept at ice temperature. At $5^{\circ}C$, histamine started to accumulate slightly during late storage in the samples irradiated at low doses. Even in the unirradiated samples, the histamine accumulation did not exceed the level of 10.0 mg per 100g meat until the 28th day at $5^{\circ}C$. At $10^{\circ}C$, however, histamine accumulation in the unirradiated was phenomenal from very early storage and the values reached 827.5 mg per 100g meat by the 35th day, while histamine accumulation in the irradiated was severely suppressed. Irradiation invariably brought about a significant reduction of initial microflora, disrupting normal spoilage pattern. This was reflected in the suppression of not only histamine but also total volatile bases and trimethylamine accumulation during post-irradiation storage at refrigerated temperatures.

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Impacts of Oyster Shell and Peat Treatments on Soil Properties in Continuous Watermelon Cropping Greenhouse Plots (패화석 및 이탄 처리가 수박 연작지 토양의 특성에 미치는 영향)

  • Ahn, Byung-Koo;Lee, Jin-Ho;Lee, Young-Han
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.4
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    • pp.438-445
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    • 2010
  • Soils in continuous monoculture cropping system generally have a number of physical and chemical problems. Thus, we studied to investigate selected soil properties in continuous watermelon cropping plots with applications of different soil management practices: 1) conventional farming practice (CFP), 2) chemical fertilizer management practice (FMP), the FMP with different amounts, 0.5, 1.0, and 1.5 t $ha^{-1}$, of oyster shell meal application (FMP-OS 0.5, 1.0, and 1.5 t $ha^{-1}$), and 3) the FMP with different amounts, 2.0, 3.0, and 4.0 t $ha^{-1}$, of peat application (FMP-PT 2.0, 3.0, and 4.0 t $ha^{-1}$) and also to evaluate watermelon quality. Soil pH slightly increased only in the FMP-OS 1.5 t $ha^{-1}$ plot, while it was not changed or decreased a little in other plots. The contents of soil organic matter (SOM) expectedly increased in the FMP-PT plots, whereas it markedly decreased in the FMP-OS plots. The concentrations of exchangeable cations, $Ca^{2+}$, $Mg^{2+}$, and $K^+$, in soils were mostly dropped down in most of the FMP and FMP-PT plots. Otherwise, the exchangeable $Ca^{2+}$ concentration increased a bit in the FMP-OS plots. Also, the concentrations of water-soluble anions, $NO_3^-$, $Cl^-$, ${SO_4}^{2-}$, and ${PO_4}^{3-}$, in soils mostly declined in all the plots applied with the different management practices during the study years. Due to the cation and anion decreases, the electrical conductivity (EC) values in the soils were greatly reduced in the plots. Thus, the soil management practices applied, especially oyster shell meal and peat treatments, might be useful to control soil conditions. However, watermelon quality, such as sugar content and fruit weight, would not be associated with the soil management practices applied.

Elimination and Utilization of Pollutants - Part I Microbiological Clarification of Industrial Waste and Its Utilization as Feed Resources - (환경오염원(環境汚染源)의 제거(除去)와 그 이용성(利用性)에 관(關)한 연구(硏究) - 제(報I)1보(第). 미생물(微生物)에 의(依)한 산업폐수(産業廢水)의 정화(淨化) 및 사료자원개발(飼料資源開發)에 개(開)하여 -)

  • Lee, Ke-Ho;Lee, Kang-Heup;Park, Sung-O
    • Applied Biological Chemistry
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    • v.23 no.1
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    • pp.64-72
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    • 1980
  • Industrial wastes from pulp and food plants were treated with microorganisms to clarify organic waste-water and to produce cells as animal feed, and results were summarized as follows. (1) Waste-water from pulp, beer, bread yeast, and ethanol distillation plants contained $1.4{\sim}1.5%$ of total sugar, $0.25{\sim}0.35%$ nitrogen, and biological oxygen demand (BOD) was $400{\sim}25,000$, chemical oxygen demand (COD), $500{\sim}28,000$, and pH, $3.8{\sim}7.0$. The BOD and COD were highest in waste-water from ethanol distillation plants among others. (2) Bacterial and yeast counts were $4{\times}10^4-1{\times}10^9,\;2{\times}10^2-7{\times}10^4/ml$ in waste-water. (3) Bacteria grew better in pulp waste and yeasts in beer, bread yeast, and ethanol distillation waste. (4) Saccharomyces cerevisiae SAFM 1008 and Candida curvata SAFM 70 were the most suitable microorganisms for clarification of ethanol distillation waste. (5) When liquid and solid waste from ethanol distillation were treated with microbial cellulase, xylanase, and pectinase, solid waste was reduced by 36%, soluble waste was increased, and recuding sugar content was increased by 1.3 times which provided better medium than untreated waste for cultivation of yeasts. (6) Optimum growth conditions of the two species of yeast in ethanol distillation waste were pH 5.0, $30^{\circ}C$, and addition of 0.2% of urea, 0.1% of $KH_2PO_4$ and 0.02% of $MgSO_4$. (7) Minimum number of yeast for proper propagation was $1.8{\times}10^5/ml$. (8) C. curvata70 was better than cerevisae for the production of yeast cells from ethanol distillation waste treated with microbial enzymes. (9) S. cerevisiae produced 16 g of dried cell per 1,000ml of ethanol distillation waste and reduced BOD by 46%. C. curvata produced 17.6g of dried cell and reduced BOD by 52% at the same condition. (10) Yeast cells produced from the ethanol distillation waste contained 46-52% protein indicating suitability as a protein source for animal feed.

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Utilization of Song-Gang Stone as the Dietary Additve in Juvenile Olive Flounder, Paralichthys olivacus (치어기 넙치에 있어서 사료 첨가제로서 송강약돌의 이용 가능성)

  • 최세민;고수홍;박건준;임성률;유광열;이제희;배승철
    • Journal of Aquaculture
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    • v.17 no.1
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    • pp.39-45
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    • 2004
  • Three experiments were conducted to determine utilization of Song-Gang(equation omitted) stone as the dietary additive for growth and immune stimulant in juvenile olive flounder. In the feeding trial, four diets were formulated to contain 0, 0.5, 1.0 and 2.0% Song-Gang(equation omitted) stone per kg diet (SGS$_{0}$ , SGS$_{0.5}$, SGS$_{1.0}$ , SGS$_{2.0}$ ). Fish averaging 5.0$\pm$0.04 g (mean$\pm$SD) were fed one of four experimental diets in triplicate groups for 8 weeks. There were no significant differences in weight gain, feed efficiency, protein efficiency ratio, hematosomatic index, condition factor and survival among fish fed all the diets. In chanlenge test, fish were infected by intraperitoneal injection of 0.1 $m\ell$ bacterial suspension with Edwardsielia tarda per fish after the feeding trial. Fish fed SGS$_{0.5}$ diet have a significantly higher cumulative survival rate than did fish fed the other diets (P<0.05). In the anti-mold test, Asprrgiilus niger, Penicillium pinophiltfm, Chaeromium globosum were inoculated with Song-Gang(equation omitted) stone using ASTM G-21 method. The amount of Α. niger, Ρ. pinuphiium. C. globosum didn't increase in Song-Gang(equation omitted) stone for 4 weeks for the test period. Therefore, these results indicate that 0.5% Song-Gang(equation omitted) stone per kg diet could increase immune resistance in juvenile olive flounder and Song-Gang(equation omitted) stone could be used as the anti-mold additive in fish feed.

Strain Improvement through Protoplast Formation and Mutation of Inonotus obliquus Mycelia for Enhanced Production of Innerpolysaccharides (IPS) in Suspended Mycelial Cultures (Inonotus obliquus 의 균사체 액상배양에서 원형질체 형성과 돌연변이를 통한 단백다당체 고생산성 균주 개발)

  • Hong, Hyeong-Pyo;Jeong, Yong-Seob;Chun, Gie-Taek
    • KSBB Journal
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    • v.25 no.2
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    • pp.155-166
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    • 2010
  • Studies on the production of cell-wall bound innerpolysaccharides (IPS) (soluble ${\beta}$-D-glucan) have been performed by use of suspended myelial cultures of Inonotus obliquus. This product has promising potentials as an effective antidiabetic as well as an immunostimulating agents. As a first step to enhanced production of IPS, Intensive strain improvement programs were carried out by obtaining a large amounts of protoplasts for the isolation of single cell colonies. Rapid and large screening of high-yielding producers was possible because about fivefold higher amount of protoplasts ($2.3{\times}10^6$ protoplasts/mL) could be recovered with relatively high regeneration rates of $10^{-2}{\sim}10^{-3}$ by applying a modified filtration method, as compared to the previously used trapping method. A basic protocol necessary for UV-mutation of the protoplasts was also developed, resulting in several overproducing variants with good fermentation properties. Since the amount of IPS extracted from the mycelial cell walls of I. obliquus turned out to be almost constant per g DCW, increase in cell mass was considered the most important factor for the enhancement in IPS production. Therefore, attempts were made to screen mutant cells showing rapid mycelial growth rate in the final suspended cultures. Notably, the mutant strains showing an active cellgrowth in the preceding solid growth cultures were observed to produce higher amount of IPS in the suspended fermentations as well. A striking mutant, OBLQ756-15-5 strain, obtained from the survivors of a harsh UV-treated condition (97% death rate) was found to stably produce as high cell mass as 22 g DCW/L in the final fermentations. Currently, this strain is being tested for development of a scaled-up fermentation process for mass production of IPS.

Anti-atherogenic Effect of Isoflavone through Hypolipidemic, Anti-oxidative and Anti-inflammatory Actions in C57BL/6 Mice (C57BL/6 Mice에서 이소플라본의 지질강하, 항산화, 항염증효과를 통한 항동맥경화 효과)

  • Cho, Hye-Yeon;Yang, Jeong-Lye;Noh, Kyung-Hee;Kim, Jin-Ju;Kim, Young-Hwa;Huh, Kyung-Hye;Song, Young-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.3
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    • pp.276-283
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    • 2007
  • This study was carried out to investigate the effect of isoflavone on the atherogenic effect in C57BL/6 mice. C57BL/6 female mice, 5 weeks of age, were fed on chow diets for 2 weeks during adjustment period. Mice weighing approximately $17.9{\pm}0.9\;g$ were divided into 4 groups and were fed on the experimental diets containing isoflavone for 8 weeks. Experimental groups were control (atherogenic diet), IF-10 (atherogenic diet with isoflavone 10 mg/100 g diet), IF-40 (atherogenic diet with isoflavone 40 mg/100 g diet) and IF-100 (atherogenic diet with isoflavone 100 mg/100 g diet). Food efficiency ratio was not different among the experimental groups. Plasma triglyceride (TG) concentrations were lower after 4 weeks in isoflavone supplementation groups than in control group, whereas monocyte chemoattractant protein-1 and thiobarbituric acid reactive substances (TBARS) levels of plasma were significantly (p<0.05) decreased in the isoflavone supplementation groups in a dose dependent manner. Both hepatic TG and cholesterol levels were significantly lowered in IF-100 than control. Hepatic glutathione concentrations were higher in the IF-100 group than in the other groups. Hepatic antioxidant enzyme activities including glutathione-reductase, glutathione-peroxidase, catalase, and Mn-superoxide dismutase were significantly higher in the isoflavone supplemen-tation groups in a dose dependent manner. From the above results, it is concluded that isoflavone may reduce the risk of atherosclerosis via hypolipidemic, anti-oxidative and anti-inflammatory effects.