• Korean journal of applied entomology
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• v.61 no.1
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• pp.77-83
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• 2022

This study was conducted to determine the optimal composition of sex attractant for monitoring adults of the tobacco cutworm, Spodoptera litura, in Korea. Lures of different compositions from the two sex pheromone components the female, (Z,E)-9,11-tetradecadienyl acetate (Z9E11-14:Ac) and (Z,E)-9,12-tetradecadienyl acetate (Z9E12-14:Ac), were compared for male attraction using synthetic chemicals, rubber septa, and funnel traps in soybean and peanut fields. After sequential tests were conducted using three sets of the two components different ranges from 5:5 to 10:1, from 9:1 to 99:1, and from 9:1 to 39:1, the 19:1 ratio was identified as the most efficient composition. However, S. litura males were barely caught in the trap of the Z9E11-14Ac single component lure. The results show that both the sex attractant compounds are necessary for monitoring adults S. litura males. The higher the pheromone amount in the dispenser in the range from 0.1 to 10 mg, the more males were attracted. Furthermore, the trap height of 1.5 m was optimal for male capture.

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
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• 2005.09a
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• pp.62-62
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• 2005

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.7 no.3
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• pp.97-103
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• 1986

• The Zoological Society Korea : Newsletter
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• v.12 no.2
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• pp.117.2-117
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• 1995

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.183.1-183
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.182.2-182
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• 1994

• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.253.2-253
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• 1995

• Proceedings of the Zoological Society Korea Conference
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• 1996.04a
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• pp.52.2-52
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• 1996

• Korean journal of applied entomology
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• v.53 no.3
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• pp.231-237
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• 2014

This study was carried out to investigate the pest species and their damage on Asparagus officinalis. Field surveys were conducted in asparagus greenhouse in Jeju, for four years from 2007 to 2010. Total of 16 pest species belonging to 5 orders were found as follows: Thrips tabaci, Thrips hawaiiensis, Adoxophyes honmai, Spodoptera litura, Spodoptera exigua, Helicoverpa armigera, Mamestra brassicae, Ascotis selenaria, Ectropis excellens, Geisha distinctissima, Aphis gossypii, Myzus persicae, Anomala albopilosa, Crioceris quatuordecimpunctata, Acusta despecta sieboldiana and Deroceras reticulatum. Among them, T. tabaci frequently occurred and damaged asparagus plants during the whole growing seasons. C. quatuordecimpunctata and snails caused severe damages on asparagus shoots in spring harvest season. In mid-late seasons, aphids and lepidopteran species occurred and attacked asparagus plants. Of the lepidopteran pests, S. litura, S exigua and H. armigera belonging to Noctuidae occurred moderately and fed on asparagus plants. And the pest control strategy in asparagus cultivation was discussed based on the present results.

• Korean journal of applied entomology
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• v.56 no.1
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• pp.19-28
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• 2017

Polydnaviruses (PDVs) are a group of double-stranded DNA viruses symbiotic to some endoparasitoid wasps. Cotesia plutellae bracovirus (CpBV) is a PDV symbiotic to an endoparasitoid wasp, C. plutellae, parasitizing young larvae of Plutella xylostella. An early expressed gene, CpBV-ELP1, plays an important role in the parasitism by suppressing host cellular immunity by its cytotoxic activity against hemocytes. This study aimed to test its oral toxicity against insect pest by expressing it in a recombinant tobacco plant. A recombinant CpBV-ELP1 protein was produced using a baculovirus expression system and secreted to cell culture medium. The cell cultured media were used to purify CpBV-ELP1 by a sequential array of purification steps: ammonium sulfate fractionation, size exclusion chromatography, and ion exchange chromatography. Purified rCpBV-ELP1 exhibited a significant cytotoxicity against Spodoptera exigua hemocytes. CpBV-ELP1 was highly toxic to the fifth instar larvae of S. exigua by injection to hemocoel. It also showed a significant oral toxicity to fifth instar larvae of S. exigua by a leaf-dipping assay. CpBV-ELP1 was cloned into pBI121 vector under CaMV 35S promoter with opaline synthase terminator. Resulting recombinant vector (pBI121-ELP1) was used to transform Agrobacterium tumefaciens LBA4404. The recombinant bacteria were then used to induce callus of a tobacco (Nicotiana tabacum Xanthi) leaves and subsequent generation (T1) plants were selected. T1 generation tobacco plants expressing CpBV-ELP1 gave significant insecticidal activities against S. exigua larvae. These results suggest that CpBV-ELP1 gene can be used to control insect pests by constructing transgenic crops.