• Title/Summary/Keyword: 담배거세나방

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Bioactive Characterization of Bacillus thuriniensis subsp. kurstaki CAB133 Isolated from Domestic Soil (국내 토양으로 분리된 Bacillus thuriniensis subsp. kurstaki CAB133균주의 생물학적 특성)

  • Choi, Su-Yeon;Cho, Min-Su;Kim, Tae-Hwan;Kim, Jin-Su;Pack, Seung-Kyung;Youn, Young-Nam;Hong, Soon-Sung;Yu, Yong-Man
    • Korean journal of applied entomology
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    • v.47 no.2
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    • pp.175-184
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    • 2008
  • To screen highly active Bacillus thuringiensis isolates against Spodoptera litura (Lepidoptera, Noctuidae), 46 B. thuringiensis was isolated from 115 samples obtained from several crop soils. Especially, B. thuringiensis subsp. kurstaki CAB133 and CAB162 isolates showed 100% mortality against S. litura. $LD_{50}$ values of CAB 133, CAB162 and HD-1 strains of B. thuringiensis subsp. kurstaki were 0.089, 3.144 and $0.513{\mu}g/ml$ against 2nd larva of S. litura, respectively. The weight of 3rd larva of S. litura which were fed crystal inclusion protein $(1.267{\mu}g/ml)$ with B. thuringiensis subsp. kurstaki CAB133 was about 30 times lass than control group. CAB133 and CAB 162 strains of B. thuringiensis subsp. kurstaki which were taken a highly toxity against S. litura were analyzed by SDS-PAGE, and estimated the molecular weight of the Cry proteins. Their serological identification by H serotypes were showed B. thuringiensis subsp. kurstaki (3abc) type.

Isolation and Activity of Insect Pathogenic Bacillus thuringiensis Strain from Soil (국내 토양으로부터 곤충병원성 세균인 Bacillus thuringiensis 균주의 분리 및 생물검정)

  • Kim, Da-A;Kim, Jin-Su;Kil, Mi-Ra;Youn, Young-Nam;Park, Dong-Sik;Yu, Yong-Man
    • Korean journal of applied entomology
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    • v.45 no.3 s.144
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    • pp.357-362
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    • 2006
  • Bacillus thuringiensis strains were isolated from the domestic soil and a strain was selected that had a new host range and high toxicity against agriculture insect pest. The 142 samples of soil were sampled from the mountains, paddy fields and patches, in Daejon, Chungnam, Chungbuk and Jeonbuk and used for the investigation. Sixteen B. t strains were isolated from 12 samples among collected samples. There were 11 strains that showed toxical activity on Plutella xylostella (Lepidoptera: Yponomeutidae), 7 steins on Spodoptera litura (Lepidoptera: Noctuidae), 5 strains on Arete coerulea (Lepidoptera: Noctuidae), 5 strains on Culex pipiens pallens (Diptera: Culicidae) among the 16 isolated B. t strains. But there were not any strains that showed activity against Hyphanria cunea (Lepidoptera: Arctiidae) and Sitophilus oryzae (Coleoptera: Rhynchophoridae). And also some of B. thuringiensis strains showed insecticidal activity with 2, 3 or 4 kinds of insects. But there were also 3 strains that did not show any activities to the 6 insects which were used in the experiment. When examined with a phase-contrast microscope, the insecticidal crystal protein produced from 16 selected strains had 13 bipyramidal and 3 spherical shapes. The insecticidal bioactivity of the S. litura showed 100% mortality when there were $1.3{\times}10^{7}\;(cfu/ml)$ of CAB109 isolates.

Damage and Control of Cutworms in the Tobacco Fields (담배포장에서의 거세미나방류 피해 및 방제)

  • 김기황;오명희;김정화;백종운
    • Korean journal of applied entomology
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    • v.40 no.4
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    • pp.315-319
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    • 2001
  • Damage and control of cutworms in the tobacco fields were studied during 1998~2000. It was estimated by surveys on cutworm damage that control measures should be taken for 9.0~10.2% of the surveyed fields. However, most of the interviewed growers broadcasted granule insecticides before or during tobacco plant transplantation. The damaging period and cutworm behavior suggested that the most effective control method would be the application of emulsifiable concentrate or wettable powder around the base of tobacco plants in the fields showing more than ca. 2% of damage ratio.

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Banana Insect Pests Species and Their Damages in the Vinyl House of Cheju Island (제주도 바나나 온실에 발생한 해충종류 및 가해양상)

  • 안성복;조왕수;임성언;김택조;이문홍;최귀문
    • Korean journal of applied entomology
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    • v.29 no.1
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    • pp.6-13
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    • 1990
  • The banana insect pest species and their damages were surveyed from 9 vinyl hoses of Cheju Island on August in 18. A total of 5 pest species wre found ; banana root weevil (BRW) Cosmopolites sordidus Germar (Curculionidae) a wireworm Melonotus sp. (Elateridae), a spider mite teranychid sp. (Tetranychidae), mulberry mealybud Pseudococcus comstocki (Kuwana)(Pseudococcidae), and common cutworm Spodoptera litura (Fabricius)(Noctuidae). Among them, the BRW which attacked the rhizome of banana plant was newly recorded from Korea. It is assumed that the weevil would be introduced from Japan, Philippine or SriLan-Ka wiht the banana seedlings imported during early 1980's.

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