• Journal of Food Hygiene and Safety
• /
• v.16 no.4
• /
• pp.258-263
• /
• 2001

Campylobacter spp. isolated and identified from the raw chicken carcasses in food service, were characterized. Total bacterial counts on the skins of raw chicken were 10$^4$~10$^{6}$ CFU/g and a total of 205 strains were primarily isolated after enrichment culture and selective culture of the sample with candle and microaerophilic chamber method. Among them, twenty eight strains of Gram-negative, catalase-positive and oxidase-positive were further isolated by the determination of biochemical characteristics. Only sixteen strains of them were finally identified as Campylobacter with PCR of pA and pB primers. Nine strains, more than half of them, have grown at 42$^{\circ}C$ and $25^{\circ}C$ and seven strains defined as thermophilic Campylobacter grew not at $25^{\circ}C$, but at 42$^{\circ}C$. Therefore, more careful management of food safety for raw chicken is needed in food service. Particularly, we should concern the raw chicken carcasses with high bacteria contamination, more them 10$^{5}$ CFU/g, which possibly includes Campylobacter spp. grown at low temperature.

• Journal of Food Hygiene and Safety
• /
• v.18 no.2
• /
• pp.79-86
• /
• 2003

Most of food poisoning is frequently raised from mass catering. Especially, staphylococci takes the large part of pathogenic agents which are related to the hygienic condition. Among total 98 samples, four staphylococci were isolated from food service environment such as drinking water (A), hands (D), refrigerator and apron (E) of 5 elementary school (A, B, C, D, E) in Gyeongnam Province. These isolated strains are characterized as 1 MRCNS (Methicilline Resistant Coagulase Negative Staphylococcus aureus) and 3 MSCPS (Methicilline Sensitive Coagulase positive Staphylococcus aureus). Also, production of enterotoxin B (sob gene) were examined by PCR which has known as a big problem because of their temperature resistance. Hence, PCR was performed on isolated 4 staphylococci. The all 4 isolated Staphylococcus aureus have 477 bp of seb gene. Antibiotics susceptibility test was completed on PCR detected strains. All strains were fully resistance to ampicillin and penicillin. The drinking water of A place has resistance to oxacilline, therefore this strain turned out to be MRSA (Methicilline Resistant Staphylococcus Aureus).

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.5
• /
• pp.3176-3182
• /
• 2014

In the early 1920s Japan, a new concept of architecture emerged abruptly. Comprised of six new college graduates, Japanese Secessionist Architects, so called Bunriha Kenchikukai, proclaimed that they reject any historical baggage that Japan had so far inherited, and decided to build a brand new way for modern Japanese architecture. Their modernism differed from eclectic measurement done by earlier generations. For them, contemporary modern Japanese architecture was only copious to historic monuments of Western civilization. Bunriha architects' statement not only advocates an escape from nationalist historicism and Westernization, but also intends to resolve the dilemma in that it substitutes a technical syncretism inherent in all avant-garde movements. This paper intends to elucidate that Japanese Secessionist architecture is greatly indebted to German Expressionist architecture in terms of its formal language and avant-garde utopian idealism.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.36 no.1
• /
• pp.117-123
• /
• 2007

HPLC quantifications of fresh and cooked (steamed/microwaved) spinach, one of the most frequently consumed vegetables in foodservice operations, were carried out to determine carotenoids compositions. An S-3 $\mu$m C30 stationary phase for reversed-phase columns with diode-array detection was used to separate and quantify geometric isomers of provitamin A carotenoids in the fresh and cooked spinach. The carotenoids in fresh spinach were identified and quantified: Lutein (63.0%), $\beta$-carotene isomers (all-trans 29.6%, 9-cis 3.2%, 13-cis 1.8%, $\alpha$-carotene 0.4%, zeaxanthin 2.1%) and cryptoxanthin. Cryptoxanthin, detected in a trace amount in HPLC, was not quantified in this study. Lutein was little affected by cooking methods and frozen conditions. 9-cis and 13-cis-$\beta$-carotene isomers were major types formed during cooking. Cooking (steam/microwave) did not alter carotenoid profiles of the samples, but the amounts of carotenoids quantified were greater than those in the fresh samples. Heat treatment such as steaming increased total carotenoids contents, especially trans-$\beta$-carotene (p<0.05). The carotenoid contents of the frozen spinach increased even after the microwaved treatment (p<0.05). These increases were likely to result from the increased extraction efficiency and inactivation of enzymes capable of carotenoids degrading during the heat treatments.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2012.05a
• /
• pp.230-230
• /
• 2012

일본의 상하수도 사업은 국토교통성(하수도)과 후생노동성(상수도)으로 크게 양분되어 있으며, 환경성이 수질을 담당하는 체제로 이루어져 있다. 이는 수량과 수질을 분리하고 있는 우리나라와도 크게 다를 바가 없다. 그러나 중앙에서의 다원적인 분리와는 또 다른 차원으로 지방자치단체에서는 상하수도 통합이 급속도로 진행되고 있다. 이는 기후변화라는 지구적 환경의 변화와 더불어 해외 물산업에 대한 경쟁력이 밑바탕이 되면서 가속화되고 있다. 즉, 아시아의 신흥국을 중심으로 급격한 경제발전에 따른 인구가 증가하면서 정수장 및 하수처리장의 건설과 더불어 누수 및 도수(盜水) 문제가 현재화하면서 이에 대한 운영관리기술의 도입이 시급해졌기 때문이다. 이러한 과제를 해결할 수 있는 충분한 기술과 경험을 보유하고 있는 일본의 지방자치단체가 해외 물산업을 전제로 지자체 내의 상하수도 통합을 추진하고 있다. 본 연구는 이처럼 이원적으로 이루어지고 있는 일본 상하수도 업무의 법제도적 차이를 살펴보고, 특히 지자체를 중심으로 전개되고 있는 상하수도 통합과정의 동향과 의의를 풍중시((豊中市)토요나까시)의 사례를 중심으로 살펴보았다. 풍중시(豊中市)는 2008년 상하수도 통합을 천명하면서 기존의 운영방침에서 크게 탈각하는 정책을 취하게 되었고, 이 정책을 통하여 시의 지속가능한 발전 및 위기관리체제를 일신하고, 나아가 기후변화에의 대응력 강화에까지 그 범위를 확장하고 있다. 지자체의 상하수도 통합논의가 가속화되는 반면, 일본 중앙 정부에서는 아직 이렇다 할 움직임을 보이지는 않고 있다. 이는 부처간의 이견차이가 큰 부분인 만큼 쉽사리 제도적인 변경으로 움직이기는 어려울 것이다. 그러나 밑으로부터의 이러한 거대한 개혁움직임과 그 동인의 정당성을 확보해가고 있는 과정을 감안한다면 정부차원의 상하수도 통합이 완전히 부정되기도 어렵다. 상하수도 통합논의와 관련한 일본의 사례는 향후 우리나라 상하수도 정책을 재검토할 수 있는 계기가 될 것이다.

• Korean Journal of Clinical Laboratory Science
• /
• v.49 no.4
• /
• pp.420-426
• /
• 2017

Methicillin-resistant Staphylococcus (MRS) can be colonized in various body sites and is more frequently isolated in healthcare associated persons. This study aimed to evaluate the contamination rate of MRS in a high school environment, those living with closed life style. Staphylococcus aureus was isolated from only the hands of 2 students among a sample of 28 students, and S. aureus were susceptible to methicillin antibiotics. Coagulase negative Staphylococci (CoNS) were isolated from the hands of 26 students (26/28, 92.9%), and among them, 14 (53.8%) isolates were methicillin-resistant CoNS (MRCoNS). Among the 14 MRCoNS, S. warneri was the most common (8/14, 57%) and susceptible to most $non-{\beta}-lactam$ antibiotics, such as clindamycin, erythromycin, ciprofloxacin, tetracycline, gentamicin, and vancomycin. In a culture of 31 desks, S. aureus was not isolated but CoNS were isolated from 26 desks (26/31, 83.6%), which did not harbor the mecA gene. The other bacteria isolated from the hands and desks were Micrococcus and Bacillus spp. In conclusion, methicillin-resistant S. aureus was not isolated from the hands and desks of high school students. However, the frequency of MRCoNS harboring mecA gene were high in the hands of high school students. Therefore, to prevent and to control the transfer of infection, intensifying preventive education, such as hand washing, and active surveillance systems, such as an investigation of contamination or carrier rate of resistant bacteria are necessary.

• Resources Recycling
• /
• v.11 no.3
• /
• pp.31-36
• /
• 2002

Magnetic separation was carried out in order to improve the magnetite grade of the spent iron oxide catalyst, that was composed with magnetite, ceria and soluble alkaline salt. The recovery of magnetite from the spent iron oxide catalyst was over 99%, and the magnetite contents was upgraded to about 80% from 70% via wet type magnetic separation at 500 Gauss. This improvement was due to the removal of alkaline salt by water instead of the magnetic separation.

• Journal of Korean Society of Archives and Records Management
• /
• v.2 no.2
• /
• pp.39-68
• /
• 2002

Many problems of archival description in new environments can be solved by building archival authority databases. This study examines function and structure of archival authority records comparing with library authority records, and analyses the archival authority records in NARA system and Australian CRS System which accepts dynamic description process for archival records. Based on these investigations, some development directions for national archival authority database are recommended.

• Journal of the Korean BIBLIA Society for library and Information Science
• /
• v.27 no.4
• /
• pp.281-299
• /
• 2016

The study aims to discuss various issues on administrative information dissemination of local governments based on analysis of local legislations related to freedom of information. As a theoretical study, it reviews a history and significance of the administrative information dissemination. Furthermore, this study analyzes articles related to administrative information dissemination which are defined by local legislations. As a result, firstly, it confirms that there are a number of cases which those articles are inadequate. Secondly, this study classifies articles related to administrative information dissemination into five types, including the A type which separately contains two different administrative information dissemination contents to the D type which only describes management aspects of administrative information dissemination, and the E type which does not define any articles related to administrative information dissemination.

• Korean Journal of Food Science and Technology
• /
• v.33 no.5
• /
• pp.582-588
• /
• 2001

To control the growth of Salmonella on raw chicken supplied in food service, they were isolated and analysed for their physiological characteristics. Total viable microbe counts under the skin of the chicken amounted to 10% of numbers on their skin. Fifty one of Salmonella were isolated and identified from 75 chicken carcass samples. About 70% of the isolates showed resistance to more than four antibiotics, which indicated very high resistance among the strains ever reported in Korea. Lactic acid and trisodium phosphate(TSP) as sanitizing agent were applied to the Salmonella spp. isolates by agar diffusion method. The resistance by isolates to those sanitizing agents was compared with type strains of S. typhimurium ATCC 14028, S. typhimurium ATCC 12023, S. heidelberg and S. enteritidis. The isolates showed similar or less resistance to the type strains as for the lactic acid. However, as for trisodium phosphate known recently as a good sanitizer, 50% of the isolates showed more resistant compared with the type strains. Also, the treatment time and regeneration medium affected significantly the resistance of the isolates, which indicates that more careful test might be needed for TSP's practical application to raw chicken. Therefore, From the results, it is suggested that antibiotic treatment during breeding broiler and contamination after slaughtering should be minimized along with careful use of sanitizing agent in order to ensure more safe raw chicken supply.