• Title/Summary/Keyword: 단백질 추출

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Purification and Characterization of $\beta$-Galactosidase from Sea Urchin, Hemicentrotus pulcherrimus (성게로부터 분리한 $\beta$-galactosidase의 정제 및 특성)

  • KIM Gyu-Hyung;KIM Yong-Tae;KIM Se-Kwon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.5
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    • pp.637-644
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    • 1998
  • [ $\beta$ ]-Galactosidase was extracted from the internal organ of sea urchin, Hemicentrotus pulcherrimus The enzyme was purified 384.6-fold over the crude extract by the sequential chromatographic methods including DEAE-Sephadex A-25, CM-Cellulose, and Con A-Sepharose 4B affinity chromatography with a recovery $1.26\%$. The molecular weight of the purified enzyme was estimated approximately 94 kDa as monomeric term by SDS-PAGE and Sephadex G-150 gel chromatography. The maximum enzymatic activity was observed at pH 3.0 and $50^{\circ}C$ but the one was stable over the ph range or 3.0$\~$5.0 and below $37^{\circ}C$. The $K_m$ and $V_{max}$ values against PNPG (P-nitrophenyl $\beta$-D-galactopyranoside) were 15.0 mM and 214 $\mu$mole/min per mg protein, respectively. The enzymatic activity was activated by $Ba^{2+}$, but significantly inhibited by $DEP,\;Hg^{2+},\;Sn^{2+}$ and galactose.

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Studies on Chemical Composition of Red Algae (국내산 홍조류의 구성성분에 관한 연구)

  • DO Jeong-Ryong;NAM Yun-Ju;PARK jin-Hee;JO Jin-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.428-431
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    • 1997
  • Chemical components of red algae (Gelidium amansii, Gracilaria verrucosa, Gigartina tenella, Carpopeltis cornea, Plocamium ovicornis, Callophyllis adnata, Lomentaria hakodatensis, Gymnogongrus flabelliformis, Chondrus pinnulatus, Adinotrichia fragilis, Gloipeltis tenax and Campylaephora hypnaeoides) were examined. The contents of carbohydrate, ash, crude protein and fat in dried red algae are $40.38\~69.88\%,\;8.57\~30.23\%,\;18.11\~33.90\%\;and\;0.11\~0.90\%$ on dry base, respectively. Yields of water-soluble compound in dried red algae and contents of sulfate in water-soluble compound extracted red algae were $4.32\~55.78\%\;and\;1.65\~19.48\%$, respectively. The principal sugars of water-soluble compound extracted from red algae were galactose, 3, 6-anhydrogalactose, glucose and xylose.

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Separation and Properties of Crude Lipase Activator from Green Pepper, Capsicum annuun Lin. (피망고추(Capsicum annuun Lin.) 중의 조(粗) Lipase Activator 의 분리와 그 특성)

  • Kim, Byung-Mook
    • Korean Journal of Food Science and Technology
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    • v.22 no.1
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    • pp.13-18
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    • 1990
  • Crude lipase activator (L. Activator) was extracted with 0.85M NaCl solution from green pepper, Capsicum annuun Lin and then fractionated by 0.2 saturation with ammonium sulfate. The activity of crude L. Activator preparation $(OD_{280}=1.0)$ had proportional relation with its added amounts below 1.0ml. The L.Activator showed optimum temperature at $35^{\circ}C$. The L.Activator was very stable at the temperatures below $50^{\circ}C$ and at pH range of $7{\sim}9$, and its activities also remained 60% even at $100^{\circ}C$, 72% at pH 3, and 85% at pH 10, respectively. The activities of L.Activator decreased by most metal ions besides $Na^+,\;Mg^{++},\;and\;Ca^{++}$. The decreasing effects of heavy metal ions such as $Ag^+\;and\;Hg^{++}$ on L.Activator activity were not, however, so great as compared with the commonly known great effects of them on most enzyme activity. Crude L.Activator was separated into 4 peaks by the cellulofine column chromatography and the main active peak of L.Activator seemed to be contained in the same components as those of the activatory peak from crude L.Inhibitor.

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The Correlation of Antioxidative Effects of 5 Korean Common Edible Seaweeds and Total Polyphenol Content (한국산 5종 해조류의 항산화효과와 총 폴리페놀 함량과의 관련성)

  • Kwak, Chung-Shil;Kim, Sung-Ae;Lee, Mee-Sook
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.8
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    • pp.1143-1150
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    • 2005
  • Antioxidative activities of 5 common edible seaweeds in Korea, three brown algae (seaweed fusiforme, sea mustard, sea tangle), one green algae (sea lettuce) and one red algae (laver), were examined. The antioxidative activities of ethanol extracts from these seaweeds were examined by measuring of inhibition rates against iron-induced linoleate peroxidation, DPPH (1,1-diphenyl -2-picrylhydrazyl) radical generation and MDA-BSA (malondialdehyde-bovine serum albumin) conjugation. Sea lettuce ethanol extract showed the strongest anti-oxidative activity among them, especially in inhibition against conjugation of lipid peroxide and protein. Second to sea lettuce, laver and sea tangle ethanol extracts showed high DPPH radical scavenging activity and inhibition against MDA-BSA conjugation. However, seaweed fusiforme and sea mustard ethanol extracts did not show antioxidative activities. Sea mustard contained the highest total flavonoids (11.33 mg/g dry wt) and sea lettuce contained the highest total polyphenol (8.97 mg/g dry wt) among these seaweeds. In addition, there was strong positive correlation between the antioxidative activity and total polyphenol content in these seaweeds, suggesting polyphenol compounds may contribute to antioxidative effect of seaweeds. From these data, it is suggested to consume much of seaweeds such as sea lettuce, laver and sea tangle to prevent age-related chronic diseases, and also develope neutraceutical products using polyphenol rich fraction from sea lettuce.

Effects of Sophorae Radix Extracts on the Expression ofcell cycle regulatory porteins in Human Gingival Fibroblasts (고삼추출물이 치은섬유아세포의 세포주기 조절단백질 발현에 미치는 영향)

  • Kim, Heung-Sik;Kim, Hyun-A;Yu, Yong-Ouk;Kang, Tai-Hyun;Kim, Youn-Chul;Kim, Tak;Pee, Sung-Hee;You, Hyung-Keun;Shin, Hyung-Shik
    • Journal of Periodontal and Implant Science
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    • v.30 no.4
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    • pp.869-885
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    • 2000
  • Fibroblasts are major cellular components of gingiva and periodontal ligament. They regulate the healing process after surgery or injury. Recently, many natural medicines, whose advantages are less side effects and possibility of long-term use, have been studied for their capacity, their anti-bacterial and anti-inflammatory effects and regenerative potential of periodontal tissues. Sophorae radix have been traditionally used as an anti-bacterial and antiinflammatory drug in oriental medicine. The purpose of present study was to investigate the effects of Sophorae radix extract on cell cycle progression and its molecular mechanism in human gingival fibroblasts. Sophorae radix extracts($100{\mu}g/ml$) notably increased cell proliferation and cell activity in the human gingival fibroblasts as compared to non-supplemented controls. There was an increase in the S phase and a decrease in the G1 phase in $100{\mu}g/ml$ of Sophorae radix extracts group as compared to non-supplemented controls. The level of cyclin E and cdk 2 protein in test group was higher than that of control groups. But that of cyclin D, cdk 4, and cdk 6 was not distinguished from controls. The level of p53 protein in test group was lower than that of controls, whereas that of p21 was not different. The level of pRB protein in test group was higher than that of controls, whereas that of p16 was lower. These results indicate that the increase of cell proliferation by Sophorae radix extracts may be due to the increased expression of cyclin E and cdk 2, and the decreased expression of p53 and p16 in human gingival fibroblasts.

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Studies on Polymorphism of Transferrin of Serum Proteins in Tilapia (Oreochromis niloticus) (Tilapia(Oreochromis niloticus)의 혈청과 단백질의 transferrin의 다형현상에 관한 연구)

  • Sim Un-Hwa;Yoon Jong-Man;Kim Kye-Yung;Park Hong-Yang
    • Journal of Aquaculture
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    • v.2 no.1
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    • pp.9-20
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    • 1989
  • This study was taken to isolate transferrin fractions from the sera of tilapia(Oreochromis niloticus) by physico-chemical analyses such as the rivanol precipitation, iron-staining method, SDS-polyacrylamide gel electrophoresis and $^{59}FeCl_3$ autoradiography, and to calculate gene frequencies by using Hardy-Weinberg Law. The results obtained in this experiment were summarized as follow : 1. The transferrin fraction is composed of several components possessing relative lower electrophoretic mobilities and higher molecular sizes than the albumin components. 2. When different staining method was compared with transferrin in band, it was not found difference. 3. It was concluded that the optimun ratio of rivanol to serum was 2 : 1 and this ratio was used in all further fractionation. 4. The molecular weight of transferrin component was about 70,000 $\pm$ 2,000. 5. Tilapia transferrin fractionations were found to be polymorphic. 6. There transferrin variants(A, B and C) have been found in tilapia(Oreochromis niloticus) and Tf types were assumed to be controlled by three codominant alleles Tf A, Tf B and Tf C. Six different phenotypes can be theoretically expected Tf AA, Tf AB, Tf AC, Tf BB, Tf BC, and Tf CC. Only five types of these were observed and Tf CC types(homozygotes) was not found. 7. The frequencies of the three allele Tf A, Tf B and Tf C were 0.795, 0.15 and 0.055 respectively.

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Determination of the Antioxidant Capacity of Korean Ginseng Using an ORAC Assay (ORAC Assay 에 의한 인삼의 항산화 활성 연구)

  • Kim, Sung-Hwan;Kim, Young-Mok
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.3
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    • pp.393-401
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    • 2007
  • This study was performed to investigate the antioxidant activity of Korean ginseng using an ORAC(Oxygen Radical Absorbance Capacity) assay. Four fractions each (80% ethanol, ethyl acetate, water saturated 1-butanol, and water) were obtained from different ginseng samples (White Ginseng: ; 6 yrs-., 5 yrs-., ; Cork Ginseng: ; 5 yrs-., 4 yrs-.). The saponin content of each fraction was quantified by LC/MS, and the antioxidant capacity of the ginseng was measured by the ORAC assay. The ORAC method, which was recently validated using automatic liquid handling systems, has been adapted for manual handling with the use of a conventional fluorescence microplate reader. Furthermore, the ORAC assay provides a direct measure of hydrophilic chain-breaking antioxidant capacity against peroxy radical, which is the exiting and emission of 2,2'-Azobis (2-methylpropionamidine)-dihychloride (AAPH). As a result of our experiments, ginsenosides Rg1 and Rb1 were the two major saponins found in the ginseng samples, and Rc, Rb2, Re, Rd, Rg3, and Rh1 were detected in a small quantities. For the antioxidant capacities of the fractions (80% ethanol, ethyl acetate, butanol, and water), we found that the organic solvent fraction had similar antioxidant capacities, and were higher than the capacity of the water fraction. When determining the similarities in each fraction, only the ethyl acetate fraction showed similarity compared to other fractions (p>0.05). The antioxidant capacity of ginseng may come from phenolic compounds and some nonpolar saponins. However, based on the results of this study, we hypothesize that some acidic polysaccharides and other biological components may contribute to its antioxidant capacity. Additional research is required to determine other possible biological response modifiers that contribute to the antioxidant capacity of ginseng.

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Tuber Enlargement and Chemical Components of Yams (Dioscorea opposita Thunb.) (둥근마(Dioscorea opposita Thunb.)의 괴경비대 및 성분특성)

  • Park Byoung Jae;Park Ju Hyun;Kim Sun Lim;Park Cheol Ho;Chang Kwang Jin
    • Korean Journal of Plant Resources
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    • v.18 no.1
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    • pp.161-168
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    • 2005
  • Tuber yield and content of general component and diosgenin which is a main bioactive property were investigated in order to determine the growth characteristics of round typed yam(Dioscorea opposita L.) and the potential of artificial culture at Suwon, Korea. Tubers of round yam were initiated to form at 60 days after planting and then enlargement of tubers lasted by 160 days after planting. Compared to short typed yam(108g), tuber weight of round yam was higher(127g) on the basis of dry weight at 200 days after planting. In comparison of general component between round yam and short yam, protein of round yam$(3.62\%)$ was higher than short yam$(2.10\%)$. Water content in round yam$(64.5\%)$ was lower in short yam$(79.4\%)$, indicating a higher dry weight ratio of round yam. Hardness of round yam was 2787.6 while short yam showed about two times higher hardness(4946.9). Lightness was higher in round yam(77.4). In tuber extracts analysis, diosgenin content was respectively $3.32\%$ in round yam and $2.61\%$ in short yam.

Studies On the Major Components and Antioxidative Properties of Whole Fruit Powder and Juice Prepared from Premature Mandarin Orange (미숙 온주밀감을 이용한 통 밀감 분말과 착즙액의 성분함량 및 항산화성에 관한 연구)

  • Kang, Yeung-Joo;Yang, Min-Ho;Ko, Weon-Jun;Park, Seung-Rim;Lee, Bong-Gyu
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.783-788
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    • 2005
  • Major components and antioxidative properties of whole fruit powders and juices prepared from premature mandarin, Citrus unshu, were investigated. Dietary fiber, flavonoid, and organic acid contents greatly increased in samples with lower degree of fruit maturity (August) than higher ones (October), and in whole fruit powders than extracted juices, whereas free sugar contents, especially sucrose content, increased in fruits with higher degree of maturity than lower ones. Antioxidative properties were not affected by degree of fruit maturity, whereas total polyphenol content and electron-donating abilities were higher in extracted juices than whole fruit powders. Nitrite-scavenging abilities of all samples were similar except at pH 6.0.

Chemical Characteristics and Physiological Activities of Plums (Oishiwase and Formosa) (자두의 화학적 특성 및 생리활성)

  • Jung, Gi-Tai;Ju, In-Ok;Choi, Dong-Geun;Jeong, Jong-Sung;Ryu, Jeong;Ko, Bok-Rai;Choi, Joung-Sik
    • Korean Journal of Food Science and Technology
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    • v.37 no.5
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    • pp.816-821
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    • 2005
  • Chemical characteristics and physiological activities of plums (Oishiwase and Formosa) were evaluated. Proximate composition of plums consisted of (w/w) 1.9-6.2% protein, 2.3-7.1% fat, 3.5-4.1% ash, and 84,1-88,7% carbohydrate. Organic acids, such as oxalic, malic, succinic, and acetic acids were detected, except in Oishiwse acetic acid was not detected. Free sugars consisted of sorbitol, glucose, fructose and sucrose. Total fiber and total phenolics compounds of plum rind were higher than those of flesh. Electron-donating abilities of rind ethanol extracts were higher than those of BHA and tocopherol, and were twofold higher than those of flesh extracts. Nitrite-scavenging abilities of rind and flesh extracts were significantly higher (over 97%) at pH 1.2 and 3.0. Tyrosinase-inhibitory activities ranged 88.5 to 100%. SOD-like activities of all extracts were weak at 22.7 to 27.2%.