• Journal of Nutrition and Health
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• v.25 no.6
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• pp.485-491
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• 1992

The effects of varying doses(1, 4 and 10mg/kg body weight/day) of testosterone propionate (TP) on body weight gain and composition and energy and muscle protein metabolism were investigated in female rats. TP had no effect on food intake at any dose but injection of 1mg/kg resulted in an in crease in body weight gain which was associated with increases in body protein and fat. At higher doses(4 and 10mg/kg) body protein content was still increased but body fat was not affected. Increases in energy gain and gross energetic efficiency were observed at a dose of 1mg/kg but neither parameter was affected at other doses. The mass protein and RNA content of gastrocnemius muscle were incerased by TPbut the ratio of RNA to protein and the rate of muscle protein synthesis measured in vivo were not affected at any dose of TP The results indicate that the effect of testosterne on body composition are highly dose-dependent and the anabolic action of testosterone is not through stimulation of protein synthesis.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.2
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• pp.73-83
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• 1983

This experiment was conducted to study effects of different sources of protein, and of the different combination ratios of the two protein supplemented or unsupplemented with methionine on the growth and body metabolism of the growing male rats. Casein and isolated soyprotein (ISP) were combined at different ratios of 100:0, 80:20, 50:50, 20:80 and 0:100 to supply the constant levels of dietary energy and protein of 3,600kcal/kg and 20%, respectively. Each combination was supplemented with either 0 or 0.3% methionine. Results obtained in these experiments were summarized as follows: The body weight and weight gain in the ISP diet were the lowest, but were increased with the increase of ratios of casein to ISP. The body weight gain in the ISP diet supplemented with methionine was similar to that in the combination diets of casein and ISP. The combination diets of casein and ISP supplemented with methionine had the higher weight gain than the casein diet. The food intake in the casein diet was the highest, and was increased with the increase of ratios of casein to ISP, and was lowered with methionine supplementation. The food intake in the ISP diet was the lowest and was not increased with methionine supplementation to the ISP diet. The food efficiency ratio of the combination diets of casein and ISP was improved compared to those of either the casein or the ISP diets. The food efficiency ratio was improved in the diet of either casein of ISP supplemented with methionine, but was not improved in the combination diets of casein and ISP with methionine supplementation. The gross energy intake had direct relation with the food intake of rats and the relation was increased either in the casein diet of with the increase of the ratios of casein to ISP. The energy efficiency and protein efficiency ratios were improved in the diet of either casein of ISP by the supplementation of methionine, and the effect of methionine supplementation of the energy efficiency and protein efficiency atio was increased with the increase of the ratios of ISP to casein. The nitrogen intake and urinary nitrogen excretion of the casein diet were the highest and those of the ISP diet were the lowest. They were increased with the increase of ratios of casein to ISP. The nitrogen balance and retention were not significantly among the treatments. The gross energy intake and fecal and urinary energy were the highest in the casein diet but the digestible and metabolizable energy and the digestibility and metabolizability of energy were not significantly different among the treatments. The body fat content of rats was increased by supplementation of methionine but the body protein content was decreased (r=-0.65, p<0.01). The body fat content of rats was negatively correlated with body moisture content (r=-0.83, p<0.01). The liver weight was highly correlated with the carcass weight (r=0.79, p<0.01), and was increased by the methionine supplementation and by the increase of the ratios of casein to ISP.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.90-91
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• 2002

In order to study the effect of dietary krill meal on the performance and the utilization of protein and energy, broiler chicks fed on the experimental diets containing 0.0%, 0.5% and 1.0% krill meal, respectively, and injected Salmonella typhimurium lipopolysacharide(LPS) (Immunological stress) in 2nd week of age during 3 weeks of experimental period. Dietary krill meal did not affect daily gain and feed efficiency but the immunological stress lowered daily gain and feed intake and nitrogen balance and increased liver and spleen weight and the energy utilization of bird. The uric acid excretion was accelerated by immunological stress.

• Journal of the Korean Society of Food Science and Nutrition
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• v.12 no.3
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• pp.264-272
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• 1983

In order to investigate the effect of dietary protein and energy on growing female and male rats, Sprague-Dawley 90 female rats and 54 male rats of 3 weeks old weighing approximately 70-80g and 54-75g, respectively, were subjected feeding trials for 8 weeks and then subsequently to metabolic trials for 2 weeks. Three dietary energy levels(3,200, 3,600, 4,000 kcal/kg) were employed and each energy level contained three protein levels (15, 25, 35% of 3600 kcal ME/kg) and three rat levels (10, 20, 40% of 3,600 kcal ME/kg) by addition of an appropriate amount of carbohydrate and the following result were obtained. As the protein level was increasing, digestibilities of dry matter and carbohydrate tended to decrease whereas that of protein was slightly increasing. On the other hand, digestibility of fat was always very high regardless of the level of protein but that tended to be slightly improved as the level of energy or rat increased. The digestibilities of female and male rats tended to be same. The digestibilities of dry matter, crude protein, crude fat and carbohydrate were 83%, 90%, 96% and 93%, respectively, and they were neither affected by protein and energy levels nor observed differently depending upon the sex. Nitrogen retention of female and male rats were best for LPHE ration. In other words, both nitrogen retention was improved as the level of energy increased and the level of protein decreased. The gross energy intake was high at low protein level in female rats and at medium protein level in male rats. That tended to decrease as the level of energy increased in female rats whereas that was not affected by the level of energy in male rats. The metabolic energy efficiency was highest for LPHE ration in female rats and for LPME ration in male rats.

• Korean Journal of Poultry Science
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• v.6 no.2
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• pp.83-90
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• 1979

In order to evaluate the effect of lipids on the utilization of the energy and Protein of the diet, the isocaloric and isoprotein diets added with mutton fat, tallow, and lecithin at the levels of two percent and five percent were prepared. The growth of chicken and the feed efficiency were observed on hatched chicken which were fed a commercial diet for the first week, with starters for the next four weeks, and with finisher diets for the last three weeks respectively during the eight weeks of the experiment. Growth rate, feed efficiency, and average body weight gain per day were similar among the chicken fed diets containing various lipids or at the levels of two percent and five percent. The metabolizable energy of the diets was found to be from 2.713kcal to 3.059kcal per gram, and the metabolizability of the gross energy was from 62 percent to 70 percent. Also the metabolizability of basal diet was higher as compared with the diets containing lipids. And the lower energy utilization was found in the broilers fed diets containing five percent lipids than those of two percent, showing not significant difference between them. Daily metabolizable energy of three week old birds ranged from 132kca1 to 168kcal which resulted in also a tendency of lower values in the five percent diets. And daily retained nitrogen of birds ranged from 0.631 gr to 0.738 gr, which showed an increasing tendency on the birds fed five percent mutton fat and tallow in comparison with those of two percent. But the lecithin brought about a reverse tendency. The digestibility of crude fats was found to be from 48 percent to 76 percent, but not significant difference among diets plus lipids or at the levels of two percent and five percent. The birds fed five percent mutton fat and tallow showed a tendency of lower digestibility as. compared with those fed two percent, but the similar results were found between two percent and five percent lecithin fed to the birds. Then, it was tentatively concluded that the effect of the mutton fat on the energy and protein utilization was similar to tallow but resulted different tendency with lecithin.

• Korean Chemical Engineering Research
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• v.57 no.1
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• pp.85-89
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• 2019

Cell-free protein synthesis utilizes the translational machinery in a cell extract. Unlike the conventional cell-based expression methods, not being affected by the conditions for cell growth, cell-free protein synthesis enables flexible manipulation of individual factors affecting the efficiency protein biosynthesis. However, the high cost and low stability of the energy sources to regenerate ATP have limited the use of cell-free synthesis for large-scale production of recombinant proteins. One of the approaches to address this problem is to use glucose as an alternative energy source to regenerate ATP through the glucose-metabolizing pathways in a cell extract. In this study, in an attempt to improve the efficiency of ATP regeneration by reinforcing oxidative phosphorylation process, we supplemented with cellular lipids to a glucose-fueled reaction mixture for cell-free protein synthesis. As a result of the lipid supplementation, the productivity of chloramphenicol acetyltransferase in a cell-free synthesis system using glucose increased more than 6 fold compared to when the lipid was not supplemented.

• Korean journal of applied entomology
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• v.55 no.1
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• pp.53-62
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• 2016

The purpose of this study was to analyze the effect of grasshopper (Oxya chinensis sinuosa) powder ingestion with/without aerobic exercise (treadmill running) on energy metabolism. To achieve this purpose, 28 Institute of Cancer Research (ICR) mice were divided into four groups: normal diet control group (CON), a normal diet with exercise control group (COEX), a grasshopper powder-supplemented diet group (GH), and a grasshopper powder-supplemented diet with exercise group (GHEX). Duration of the powder ingestion and aerobic exercise training were 6 weeks. Body weight gain ratio was not significant. Fat mass significantly decreased in GH and GHEX. There were no changes in blood glutamic oxaloacetic transaminase and glutamic pyruvic transaminase levels between groups. Glucose transporter type 2 and glucose transporter type 4 protein levels were not significantly different between groups. Fibronectin type III domain-containing protein 5 level was the highest in GHEX. AMP-activated protein kinase level significantly increased in GHEX compared to the levels in the other groups. Glycogen synthase kinase 3 beta protein level was reduced in GHEX compared to that in CON. These results suggest that grasshopper powder ingestion and endurance exercise training influence energy metabolism.

• Korean Journal of Poultry Science
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• v.30 no.2
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• pp.73-81
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• 2003

This experiment was conducted to investigate the effects of dietary energy and protein levels on the growth rate of egg breeder pullets. A total of 360 Hy-Line Brown pullets aged 2 to 6 weeks (Phase I) were fed 5 rations differing in dietary protein (17, 19 and 20%) and energy (2,800, 2,950 and 3,050 kcal/kg, TMEn) leveIs for a period of 5 weeks and those aged 6 to 10 weeks (Phase II) were fed 5 rations differing in dietary protein (15, 16 and 17%) and energy (2,800, 2,900 and 3,000 kcal/kg, TMEn) levels in order to evaluate the optimum dietary energy and protein leveIs for egg breeder pullets reared in cages. Their body weight gains were significantly influenced by the dietary protein levels (P<0.05). The dietary energy levels did not greatly affect the growth performances throughout the experimental period. The low energy and protein regimen based on NRC requirement (control) was found to produce smaller pullets and lower tibia bone measurements as compared to the higher regimen groups. With the increase in dietary energy and protein levels, tibial bone strength and ash content also gradually increased (P<0.05). There were no significant differences in the flock uniformity among the treatments. These results indicate that increases in dietary energy and protein levels above the NRC requirements appeared to be more effective in obtaining the optimal growth and bone developments of egg breeder pullets reared in cage.

• Journal of Nutrition and Health
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• v.15 no.4
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• pp.301-312
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• 1982

A series of growing and digestion trials was conducted using Sprague- Dawley weanling male rats in order to determine the effects of two protein sources (casein and ISP (isolated soyprotein) ), three protein levels (10, 20 and 30%), and two feeding patterns (ad libitum and meal feeding) on the growth, protein and energy metabolism, and body composition of rats. The total energy level of experimental diets was kept constant in 3,600 kcal ME /kg diet. The results were as follows : 1) The amount of food intake and the weight gain of meal-fed group were lower than those of ad libitum group. Though the intake of meal-fed group on 20 and 30% casein diet was only 85% of ad libitum group, it was able to gain as much as ad libitum group. 2) There were no significant differences in the food efficiency ratio (FER) and the energy efficiency (weight gain per 100 kcal GE intake) between ad libitum and meal feeding group. The FER and the energy efficiency of 20 and 30% casein diets of meal-fed group were greater than those of ad libitum group. 3) Though the gross energy intake (GE ), the digestible energy (DE) and the metabolizable energy (ME) tended to be lower at meal-fed group, the DE/GE and the ME/GE ratios for meal-fed group were the same as those for ad libitum. 4) Though meal- fed group fed less amount of nitrogen than ad libitum group, there were no differences in nitrogen balance and the retention of rats among the treatments. Actually meal-fed group retained more nitrogen than ad libitum group at the levels of 20 and 30% dietary protein. 5) After growing and digestion trials, the body composition of rats was constant among all treatments. Significantly high negative correlation coefficient (r = -0.77) was found between the body fat content and the body moisture content. Consequently, this study suggests that meal- fed group on 20 and 30% casein diets has shown more effective utilization of the ingested food and energy than ad libitum group, and increasing tendency of weight gain and the body fat deposition. Those influences of meal feeding pattern in rats were more effective on the casein diet than on the ISP diet.

• Korean Journal of Poultry Science
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• v.16 no.3
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• pp.139-147
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• 1989

An experiment was carried out to investigate the effects of dietary energy levels and protein feeding systems on the performance of laying hens. Total 960 White Leghorn pullets were alloted in 10 treatments consisted of 1 metabolizable energy levels (2,900 and 2,600 kcal/kg) and 5 protein feeding systems (17-17-15%, 17-15-13%, 15-15-15%15-15-13% and 15-13-13% in phase I, II and/or III, respectively) from 20 to 80 weeks or age. As metabolizable energy level increased from 2,600 to 2.900 kcal/kg of diet, e99 Productions in phase I and II were not different but decreased in phase III. Egg weight was not different, but daily feed intake and viability were decreased in phase I, II and III. Feed conversions in phase I and II were improved but in phase III it was not different. On the other hand, as dietary Protein level increased from 13 to 17%, egg production and egg weight were increased, and feed conversion was improved , but daily feed intake and viability were not affected by the dietary protein level. For the laying period of 60 weeks, metabolizable energy level of 2,600 kcal/kg of diet and 17, 15 and 13% dietary protein levels in phase I, II and III, respectively, were considered to be adequate to support the optimum productivity.