• Korean Journal of Oriental Medicine
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• v.17 no.2
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• pp.169-172
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• 2011

Traditional medicines usually use combinational formula that is prepared by mixing different varieties of medicinal herbs and boiling them in water to yield a decoction. In recent years a modified method has been proposed and practiced wherein the individual herbs are boiled with water separately and later these extracts are mixed together for use. This practice has lead to a constant controversy concerning the chemical and biological equivalence between the preparations arriving out of these two methodologies. In this research, we attempted to study the effects of traditional and individual preparation of Ssanghwa-tang (ST) to evaluate their equivalence in terms of its antioxidant activity and polyphenol content. The study results indicate that the polyphenol contents as well as the extraction yields of these two preparations were very similar to each other.

• Journal of Oriental Neuropsychiatry
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• v.16 no.1
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• pp.19-41
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• 2005

Object : This study was designed to asses the effect of Sunghyangjungkisan-ga-pogongng and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation, Mouse neuroblastoma 2a (N2a) cells were treated by SHJG+P and herbs. Result : 1. SHJG+P was effective on LDH assay of hypoxia and reoxygenation. 2. The herbs were generally effective on LDH assay of hypoxia and reoxygenation. In MTT assay of hypoxia JP and GC were effecctive. In LDH assay of hypoxia all of herbs were effective. DMH, BC, SY, NS were more effective than other herbs. In LDH assay of reoxygenation KH, BH, BBR, DMH were especially effective. In MTT assay of reoxygenation most of herbs were not effective. But GC, SY, BH, JP were effective. Conclusion : The results imply that SHJG+P and all of berbs may have protective effect on dementia and GC, SY, BH, JP may have protective effect.

• The Journal of Internal Korean Medicine
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• v.39 no.4
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• pp.592-604
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• 2018

Objectives: This study is to review the effect of Korean medicinal herbs on treatment of benign prostatic hyperplasia induced in rat models, as reported in domestic and foreign journals. Methods: Six electronic databases (EMBASE, PubMed, Oasis, RISS, CENTRAL, and Koreankt) were searched with terms including benign prostatic hyperplasia to identify study reports on treatment of benign prostatic hyperplasia impairment with Korean medicinal herbs. After selecting several studies, the analysis focused on items reflected in the diagnosis of benign prostatic hyperplasia, such as prostate weight, thickness of the prostate epithelium, and prostate specific antigen. Results: Six studies were reviewed. Testosterone propionate was used as a benign prostatic hyperplasia induction material in all the included studies. Cinnamomum verum (CV), Cynanchum wilfordii (CW), Ponciri fructus (PF), Quisqualis indica (QI), Acorus gramineus (AG), and Melandrium firmum (MF) had reduced prostate weight statistically significantly. The QI gave a better response than finasteride in terms of reducing epithelium thickness, and the response was statistically significant. The prostate specific antigen level was lower in the group treated with CV than in the control group. Conclusions: CV, CW, PF, QI, AG, and MF had distinct therapeutic effects. However it is difficult to determine which of these is better by comparing them numerically because the observation items evaluated in a rat model of benign prostatic hyperplasia.

• Korean Journal of Veterinary Service
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• v.33 no.4
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• pp.367-373
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• 2010

Investigations for hematologic and biochemical values for Gyeongju DongGyeong dogs were performed. This study were conducted to determine normal blood and serum chemical values on DongGyeong dogs. Normal blood and serum chemical values were studied in 47 healthy dogs (male 28, female 19). The hematologic and serum chemistry results were confirmed by age, sex, shape of tail. The hematological values showed no difference from Korea native dogs. The hematocrit values ($49.0{\pm}8.1$(%)) of DongGyeong dogs (2-3 years group) were significantly higher than other dogs. The mean levels of MCV ($61.6{\pm}3.8({\mu}m^3)$) in DongGyeong dogs were lower than general dogs. The levels of cholesterol($220{\pm}80.5$(mg/dl)) and inorganic phosphorus ($3.6{\pm}1.0$ (mg/dl)) of DongGyeong dogs (2~3 years group) were significantly higher than Korea native dogs. Totally concentration of triglyceride ($66.2{\pm}17.0$ (mg/dl)) of DongGyeong dogs were significantly lower than Korea native dogs. The mean values of BUN were increased with age but no significantly. In conclusion, data obtained from this study may be valuable as a standard for interpretation of the results in hematologic and biochemical analysis of Gyeongju DongGyeong dog populations.

• Journal of Oriental Neuropsychiatry
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• v.16 no.2
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• pp.89-112
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• 2005

Object : This study was designed to assess effect of Chungpesagan-Tang and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation. Method : Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation. Mouse neuroblastoma 2a (N2a) cells were treated by Chungpesagan-Tang and herbs. Result : In MTT assay of hypoxia all of herbs were almost ineffective and Hubak was a little effective. 2. In MTT assay of reoxygenation most of herbs were not effective. But Hubak was some effective. 3. In LDH assay of hypoxia all of herbs were effective. Especially Chungpesagan-Tang were equally effective on all of concentration. 4. In LDH assay of reoxygenation all of herbs were generally effective. Especially Chungpesagan-Tang and Baekji were highly effective and Kilkyung was also effective on low concentration. 5, The herbs were generally effective on LDH assay of hypoxia and reoxygenation. Conclusion : The results suggest that Chungpesagan-Tang and all of herbs may have protective effect on condition of oxidative stress and can be applied on the development of a new medicine for neurodegenerative disease like dementia.

• Korean Journal of Oriental Medicine
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• v.5 no.1
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• pp.59-72
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• 1999

Vibrio are become prevailing if superficial temperature of ocean is raised and their activities of area are expanded and most of ocean creatures(fishes, oysters etc) are polluted with vibrio. The one who has taken these poluted fishes and aysters uncooked caused foodpoisoning and diarrhea from Vibrio. Frequencies of these diseases breakout is disposed in westsea shore of Korea. According to ancient and traditional Korean medical book -${\ulcorner}$Bangyak Happyeon${\lrcorner}$(Collection of Local Medicines, 1884) - and their single prescribes, we carried out experiment check the activities of natural medicinal effects on Vibrio parahemolyticus. The prescriptions of trial materials are processed from extraction boiling water and 80% methanol and followed freeze dried and adsorbed to every discs in dosage of 10mg. Gentamycin of 10mg were used for control. The result of compound prescription displayed special diseases in antimicrobial activities of boiling water and MeOH extraction compared with control. In compound prescription, extraction MeOH of Sashinhwan(clear zone : 17mm) presented extraordinaire antimicrobial activity. In single prescription, extraction of boiling water(clear zone : 16mm) and MeOH(clear zone : 18mm) of Fructus Chebulae presented extraordinaire antimicrobial activity. The MBC of Fructus Chebulae extracts was expressed in boiling water(1.28mg/ml) and MeOH(0.64mg/ml).

• Journal of Physiology & Pathology in Korean Medicine
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• v.30 no.1
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• pp.40-46
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• 2016

Many medicinal plants have been used for the treatment of edema, jaundice, and gonorrhea in traditional Oriental medicine. This screening study was designed to search the positive inotropic effects of herbal extracts in beating rabbit atria. Aquarius extracts of twenty six herbs were examined in atrial mechanical dynamics such as pulse pressure and stroke volume and atrial natriuretic peptide (ANP), one of the main hormones involved in the regulation of the body fluid and blood pressure homeostasis in perfused beating rabbit atria. Sophora flavescens Ait., Rheum officinale Baill., Acorus gramineus Sol., Chelidonium majus L., Pulsatilla koreana Nakai., Reynoutria japonica Houtt., Euphorbia lathyris L., Pyrrosia lingua (Thunb.) Farwell, Poncirus trifoliata Rafin., Anemarrhena asphodeloides Bunge, Kochia scoparia Schrad. significantly increased stroke volume and pulse pressure. However, those herbal extracts were not induced ANP secretion. We clarified the eleven herbal extracts for the positive inotropic effect independent of ANP secretion in beating rabbit atria. Thus these results provide a beneficial data for the treatment of the impairment of body fluid and blood pressure in traditional Oriental medicine.

• Journal of Korean Medical classics
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• v.18 no.2 s.29
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• pp.70-84
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• 2005

장석순(張錫純)$(1860{\sim}1933)$시청말민초시기적의가(是淸末民初時期的醫家), 통과대기소저(通過對其所著)${\ulcorner}$의학충중참서록(醫學衷中參西錄)${\lrcorner}$적제오기제삼권중(的第五期第三卷中), 관우(關于)'논간병치법'(論肝病治法)내용적연구(內容的硏究), 소득출적결론시(所得出的結論是), 기대간병적치법가개괄위평간법(其對肝病的治法可槪括爲平肝法), 산간법(散肝法), 화간법(化肝法), 유간법(柔肝法), 진간법(鎭肝法), 염간법(斂肝法), 보간법(補肝法), 완간법등팔법(緩肝法等八法). 평간법인위(平肝法認爲), 인지원기찰근우신, 맹아우간이유약역손(萌芽于肝而柔弱易損), 고불가관파간기(故不可關破肝氣). 차내경중(且內經中)'궐음불치(厥陰不治), 구지양명(求之陽明)'여금궤중(與金櫃中)'당선실비(當先實脾)', 도이서조간기위관건(都以舒調肝氣爲關鍵), 인차이승강탕여배비서간탕등립방(因此以昇降湯與培脾舒肝湯等立方), 차작위평간약중시료계지(且作爲平肝藥重視了桂枝). 산간법인위(散肝法認爲), 간위목장(肝爲木臟), 구유직향이성향상신전(具有直向而性向上伸展), 소이부응기성(所以符應其性), 이용신미산지(而用辛味散之), 즉가위보법(卽可爲補法). 작위산간약(作爲散肝藥), 기선용인진여맥아(其善用茵蔯與麥芽). 화간법시재간체증대(化肝法是在肝體增大), 혹간중유열(或肝中有熱), 혹간기울체(或肝氣鬱滯), 혹옹창시(或壅脹時), 작위산간달울지법(作爲散肝達鬱之法), 사용료시호(使用了柴胡), 유향(乳香), 몰약(沒藥), 차이활혈약당좌약사용적치법(且以活血藥當佐藥使用的治法), 우기중시료삼칠근(尤其重視了三七根). 유간법시근거(柔肝法是根據)'간악조희윤(肝惡燥喜潤), 조칙간체판경이간화간기즉망동(燥則肝體板硬而肝火肝氣卽妄動), 윤칙간체유화이간화간기장영정(潤則肝體柔和而肝火肝氣長寧靜)'적이론(的理論), 인위재치료중가선용당귀(認爲在治療中可選用當歸), 작약(芍藥), 백자인(柏子仁), 현삼(玄蔘), 구기자(枸杞子), 아교(阿膠), 별갑(鱉甲), 단불가장기사용(但不可長期使用). 기진간법시(其鎭肝法是), 재치료경간(在治療驚癎), 전광(癲狂), 현운(眩暈), 뇌충혈제증시(腦充血諸證時), 작위대표처방선용료진간식풍탕(作爲代表處方選用了鎭肝熄風湯). 염간법시근거기(斂肝法是根據其)'범인원기지탈(凡人元氣之脫), 개탈재간(皆脫在肝)'적독특이론(的獨特理論), 이내복탕등작위대표처방진행치료적방법(以來復湯等作爲代表處方進行治療的方法), 차대제양사용료산수유(且大劑量使用了山茱萸). 보간법시재간기허약이실기조달시사용적치법(補肝法是在肝氣虛弱而失其條達時使用的治法), 인위황기시보간적주약. 완간법시의거(緩肝法是依據)‘간고급(肝苦急), 급식감이완지(急食甘以,緩之)'지설이정적치법(之說而定的治法), 인위단미감초시대표약(認爲單味甘草是代表藥).

• Journal of Physiology & Pathology in Korean Medicine
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• v.28 no.6
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• pp.657-661
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• 2014

Gilbert's syndrome is one that shows a benign course with intermittent unconjugate hyperbilirubinemia without any evidence of hepatobiliary tract disease or hemolysis. It is often found in a health examination or blood laboratory test by chance. In particular, patients who are taking drugs, including herbal medicine should be careful for their medication due to the possibility of associations with changes in liver function because of drug metabolism, sometimes they have to quit the use of the medication for a certain period and often they should get an additional test. Two male patients increased serum total bilirubin level without other systemic symptoms in screening test for clinical herb medicine pharmacokinetics study. Therefor they was diagnosed with suspected Gilbert's syndrome. They had been calory deprivation test with 24 hours fasting state. They also performed liver function test and ultrasonogram for evaluation of hepatobiliary tract disease. Total serum bilirubin was markedly increased, especially unconjugate bilirubin level higher over the two times than base line after they had been calory deprivation for 24 hours, They was not found another abnormality all laboratory results and physical examination. This study is a report on two cases of hyperbilirubinemia, diagnosed as Gilbert's syndrome, which were found in the process of a clinical pharmacokinetic study of a decoction of medicinal herbs.

• Korean Journal of Veterinary Research
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• v.40 no.2
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• pp.221-228
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• 2000

We performed this study to determine the effect of Bu-Zhong-Yi-Qi-Tang, as a prescription of traditional Oriental medicine, and its major ingredients on jejunal crypt survival, endogenous spleen colony formation, apopotosis in jejunal crypt cells, lethality and hematological change of mice irradiated with high and low dose of Y-radiation. Bu-Zhong-Yi-Qi-Tang administration before irradiation protected the jejunal crypts (p<0.0001), increased the formation of endogenous spleen colony (p<0.05) and reduced the frequency of radiation-induced apoptosis (p<0.05). The survival rate and mean survival time of the groups treated with Bu-Zhong-Yi-Qi-Tang within 30 days after the treatment were far better than the irradiation control group. In the experiment on the effect of ingredients of Bu-Zhong-Yi-Qi-Tang, the result indicated that the extract of Rensan (Panax ginseng), Danggui (Angelica sinensis), Shengma (Cimicifuga heracleifolia) and Chaihu (Bupleurum falcatnosa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indicated that BU-Zhong-Yi-Qi-Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the protective nature of Bu-Zhong-Yi-Qi-Tang extract and its ingredients.