• Korean Journal of Food Science and Technology
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• v.21 no.1
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• pp.136-144
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• 1989

The purification of myrosinase from radish roots was performed using Concannavalin A-Sepharose affinity chromatography and gel permeation HPLC, which gave a 22-fold-purification(S.P.A.=39,000 units/mg) with 50% recovery, SDS-polyacrylamide gel electrophoresis showed a single major band, suggestive of a relatively pure myrosinase, and the M.W. of the enzyme determined on gel permeation HPLC was ca. 124K. The enzyme showed on optimum pH of 6.5 and was stable at pH 6 to 7 at room temperature, but unstable below pH 4. The enzyme possessed an optimum temperature of $37^{\circ}C$, and gave a Vmax value of $40\;{\mu}moles/mg{\cdot}min$ and a Km value of 0.12mM for sinigrin. The purified myrosinase was activated maximally by 0.6mM of ascorbic acid, but somewhat inhibited by more than 2 mM ascorbic acid. The activities of myrosinase present in the peelings and the peeled radish amounted to approximately 1,333 units/g and 140 units/g weight, respectively and the peelings contained much more myrosinase activity than the peeled radish.

• KSBB Journal
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• v.21 no.2
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• pp.133-139
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• 2006

In 'solid-phase' PEGylation, the conjugation reaction occurs as the proteins are attached to a solid matrix, and thus it can have distinct advantages over the conventional, solution-phase process. We report a case study: rhIFN-${\alpha}$-2a was first adsorbed to cation exchange resin and then N-terminally PEGylated by aldehyde mPEG of 5, 10, and 20 kD through reductive alkylation. After the PEGylation, salt gradient elution efficiently recovered the mono-PEGylate in a purified form from the unwanted species such as unmodified IFN, unreacted PEG, and others. The mono-PEGylation and its purification were integrated in a single chromatographic step. Depending on the molecular weight of the mPEG aldehyde used, the mono-PEGylation yield ranged 50-64%. We could overcome the major problems of random, or uncontrollable, multi-PEGylation and the post-PEGylation purification difficulties associated with the solution-phase process. N-terminal sequencing and MALDI-TOF MS confirmed that a PEG molecule was conjugated only to the N-terminus. Compared with the unmodified IFN, the mono-PEGylate showed the reduced anti-viral activity as measured by the cell proliferation assay. The bioactivity was reduced more as the higher molecular weight PEG was conjugated. Immunoreactivity, evaluated indirectly by antibody binding activity using a surface plasmon resonance biosensor, also decreased. Nevertheless, trypsin resistance as well as thermal stability was considerably improved.

• Journal of Soil and Groundwater Environment
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• v.12 no.1
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• pp.73-86
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• 2007

Risk-based remediation strategy (RBRS) is a consistent decision-making process for the assessment and response to chemical release based on protecting human health and the environment. The decision-making process described integrates exposure and risk assessment practices with site assessment activities and remedial action selection to ensure that the chosen actions are protective of human health and the environment. The general sequences of events in Tier 1 is as follows: initial site assessment, development of conceptual site model with all exposure pathways, data collection on pollutants and receptors, and identification of risk-based screening level (RBSL). If site conditions do not meet RBSL, it needs further site-specific tier evaluation, Tier 2. In most cases, only limited number of exposure pathways, exposure scenarios, and chemicals of concern are considered the Tier 2 evaluation since many are eliminated from consideration during the Tier 1 evaluation. In spite of uncertainties due to the conservatism applied to risk calculations, limitation in site-specific data collections, and variables affecting the selection of target risk levels and exposure factors, RBRS provides us time- and cost-effectiveness of the remedial action. To ensure reliance of the results, the development team should consider land and resource use, cumulative risks, and additive effects. In addition, it is necessary to develop appropriate site assessment guideline and reliable toxicity assessment method, and to study on site-specific parameters and exposure parameters in Korea.

• Korean Journal of Ecology and Environment
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• v.35 no.3 s.99
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• pp.198-212
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• 2002

A numerical variation and abnormalities were studied on egg bags and embryos of Korean salamander, Hynobius leechii from agricultural habitat. The teratogenic and toxic effects of fungicide benomyl were also investigated with early embryos from non-agricultural habitat. We collected 144 egg bags from agricultural region, and 3418 of early embryos were contained. The lengths of egg bags were varied from 10 to 23 cm and the most frequent length was 19 cm. The number of embryos was varied from 7 to 43, and the most frequent range was 22 to 26. Spontaneous abnormalities were occurred in 406 embryos among 116 egg bags, and 24 kinds of external abnormalities were found. Individuals showing severe external defect were histologically studied and they showed optic dyspalsia, thyroid carcinoma, somatic muscular dysplasia, partial biaxial structure, decrease of red blood cells in the heart, cephalic degeneration and intestinal dysplasia. 385 embryos from non-agricultural region were exposed to 200 nM${\sim}$ 1 ${\mu}$M of benomyl at blastula or gastrula for 12 days. All embryo were dead in the concentration of 1 ${\mu}$M (LD$_{100}$) and 75% of embryos were dead in 800nM of benomyl. Speciflc effect due to benomyl was acrania or cephalic dysplasia and this restult suggests that the benomyl inhibit stongly to the development of neural tissue. These abnormal developments may be caused by antimitotic action, inhibition of tubulin complex, destruction of microtubule, inhibitions of neurulation and closing of neural fold, and by the inhibition of the movement of neural crest cells.

• Korean Journal of Food Science and Technology
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• v.45 no.3
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• pp.285-292
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• 2013

This study was carried out to validate the safety of ametoctradin residues in agricultural commodities by developing an official analysis method. An analytical method was developed and validated using HPLC-PDA detectors. The samples were extracted with methanol, subsequently partitioned with dichloromethane and purified with florisil column chromatograph using acetone/hexane (30/70, v/v) as solvent. The method was validated by using grape, hulled rice, mandarin, and potato spiked with ametoctradin at 0.05 and 5.0 mg/kg, and pepper at 0.05 and 2.0 mg/kg. Average recoveries were 76-114.8% with relative standard deviation less than 10%, and the limit of detection and limit of quantification were 0.0125 and 0.05 mg/kg, respectively. The result of recoveries and overall coefficient of variation of the laboratory results from Gwangju regional Food and Drug Administration (FDA) and Daejeon regional FDA was accorded with Codex Alimentarius Commission Guideline (CAC/GL 40). Based on these results, this method was found to be appropriate for ametoctradin residue determination and can be used as the official method of analysis.

• The Korean Journal of Pesticide Science
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• v.10 no.2
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• pp.124-130
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• 2006

Rice plant extracts of brown planthopper (BPH) resistant rice varieties, Jangseongbyeo (JSB) and Hwacheongbyeo (HCB) at different growth stages (seedling, tillering, heading and ripening) were sequentially fractioned using hexane, ethyl ether, ethyl acetate, butanol, and distilled water. The extracts were applied to BPH susceptible rice variety, Dongjjnbyeo (DJB), to investigate the insecticidal and repellent effects against BPH. BPH insecticidal effects were not clearly observed with almost all of the extract fractions obtained from both JSB and HCB varieties for 12 h, whereas the ethyl ether and hexane extract fractions showed about 10 to 30% of BPH mortality in 24 to 48 h of application periods. An effective BPH repellent activity was found with the applications of ethyl ether extract fractions obtained from JSB variety. The extract fractions obtained from HCB variety did not show any different repellence among the various fractions. The BPH repellent effects of the extract fractions obtained at different growth stages of either JSB or HCB varieties did not show any correlations. The effect of ethyl ether fraction on BPH repellent was continually increased by 30 h after treatment and thereafter decreased. In addition, the first sub-fraction separated by a flash column chromatography eluted with chloroform:methanol (9:1, v/v) from the BPH effective ethyl ether faction in JSB variety might be meaningful to repel BPH from BPH susceptible target rice plants. The results indicated that the ethyl ether fraction obtained from JSB was higher in repellent activity than in insecticidal activity, and suggesting that there might be specific substance(s) in the first sub-fraction (sF1) of the ethyl ether fraction in JSB that could provide repellent activity against BPH.

• Korean Journal of Clinical Laboratory Science
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• v.56 no.2
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• pp.147-155
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• 2024

Gallium-68-prostate-specific membrane antigen-11 (⁶⁸Ga-PSMA-11) is a positron emission tomography radiopharmaceutical that labels a Glu-urea-Lys-based ligand with ⁶⁸Ga, binding specifically to the PSMA. It is used widely for imaging recurrent prostate cancer and metastases. On the other hand, the preparation and quality control testing of ⁶⁸Ga-PSMA-11 in medical institutions takes over 60 minutes, limiting the daily capacity of ⁶⁸Ge/⁶⁸Ga generators. While the generator provides 1,110 MBq (30 mCi) nominally, its activity decreases over time, and the labeling yield declines irregularly. Consequently, additional preparations are needed, increasing radiation exposure for medical technicians, prolonging patient wait times, and necessitating production schedule adjustments. This study aimed to reduce the ⁶⁸Ga-PSMA-11 preparation time and optimize the automated synthesis system. By shortening the reaction time between ⁶⁸Ga and the PSMA-11 precursor and adjusting the number of purification steps, a faster and more cost-effective method was tested while maintaining quality. The final synthesis time was reduced from 30 to 20 minutes, meeting the standards for the HEPES content, residual solvent EtOH content, and radiochemical purity. This optimized procedure minimizes radiation exposure for medical technicians, reduces patient wait times, and maintains consistent production schedules, making it suitable for clinical application.

• Applied Biological Chemistry
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• v.29 no.4
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• pp.407-415
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• 1986

To obtain informations on the development of the combined granular pesticides for the simultaneous control of rice insect pests and diseases which often occur at the same time or in overlapping experiments were carried out on twelve mixture pesticides of granular type formulated by extrusion method with two fungicides and three insecticides. Each of 12 mixtures was tested for physico-chemical properties and efficacy against to rice leaf blast and brown planthoppers under the laboratory conditions. All of the mixture pesticides showed acceptable physico chemical properties as granular formulation. Most of the compounds in mixture pesticides showed a tendency to have lower stabilities than those of pesticide alone, therefore, it was necessary to add stabilizer in formulation process. Isoprothiolane combinations were found very effective against leaf blast but their control effects were similar to that of isoprothiolane alone due to its high efficacy, while probenazole-carbofuran combinations showed synergistic effects for the control of leaf blast. All of the combinations showed synergistic effects to brown planthoppers. But fenthion and 3% propoxur combinations resulted comparatively low efficacies. In the consideration of their physico-chemical properties and efficacies to rice pests, carbofuran and 6% propoxur combinations were promising combined granular pesticides for the simultaneous control of leaf blast and brown planthoppers, while fenthion and 3% propoxur combinations were not available because of their low efficacies for brown planthoppers.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.1
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• pp.26-32
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• 2001

Influence of various rates of fractionated raw cow manure on hydraulic conductivity of the soil was observed. The fractionated raw cow manure(hereafter as FRCM) incorporated into soil. The hydraulic conductivity was measured for the double-layered soil while maintaining the water head by 5 cm over the soil surface. The influence on the mobility of $NO_3{^-}$-N transformed from the FRCM was analyzed. The upper layers (Wolgok series) were made with FRCM ranging from 0% to 10.4 % on weight basis for air-dried soil while the organic matter in the bottom layers (Chungwon series) was removed by combustion. The initial bulk densities for both layers were adjusted to $1.25g\;cm^{-3}$. In this experiment the $K_{sat}$ for the upper layer gradually decreased from $4.71{\times}10^{-3}cm\;min^{-1}$ to $1.2{\times}10^{-3}cm\;min^{-1}$ with increasing the rate of the FRCM from 0 % to 10.4%, while the Ksat of the bottom layer was maintained as $3.7cm\;min^{-1}$. For the double-layered soil columns, the $K_{sat}$ decreased with increasing rate of FRCM at the upper layer from $1.7{\times}10^{-3}cm\;min^{-1}$ to $8{\times}10^{-4}cm\;min^{-1}$ as the rate of organic matter increased from 0 % to 10.4 %, while it took almost 7 days to 64 days to obtain the steady state $K_{sat}$ The elution patterns of $NO_3{^-}$-N and $NH_4{^+}$-N showed that the amounts of both $NO_3{^-}$-N and $NH_4{^+}$-N rapidly approached to the maximum ranging from $14.8mmol_c\;kg^{-1}$ to $0.58mmol_c\;kg^{-1}$ as the rate of FRCM decreased from 10.7 % to 0 % which is equivalent to indigenous amount of $NO_3{^-}$-N and $NH_4{^+}$-N. And the amounts of $NO_3{^-}$-N were approximately three or four time than those of $NH_4{^+}$-N, indicating that the transformation rate of $NO_3{^-}$-N was improved by the higher FRCM rate. Thus, the ability of a soil to supply N can be predicted from its mineralization parameters and leaching potentials influenced by water flow regime in soil.