• Communications for Statistical Applications and Methods
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• v.17 no.1
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• pp.67-77
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• 2010

In multinomial scheme with step-wise sampling, maximum likelihood estimates of multinomial probabilities are improved when some frequencies are merged. In this study, for cell probabilities in a I by J independent contingency tables, exact MLE and step-wise estimation methods are applied and the results are compared using MSE and Bias.

• Journal of the Mineralogical Society of Korea
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• v.23 no.1
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• pp.85-91
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• 2010

The various forms of heavy metals in soil environments have been studied by sequential extraction method. We tested conventional Tessier sequential extraction and new ultrasound-sonication extraction methods, and compared their extraction efficiency. Total As, Cd, Cu, Pb, and Zn contents of the target soil (NIST SRM 2710 Montana Soil), by three methods (USEPA Method 3050B, KBSI Method, and ultrasound-sonication method) were all consistent with the certified values. Sequential extraction efficiency along with step-wise extraction values was similar in both Tessier method and ultrasound-sonication method. The ultrasound-sonication method took about 3 hours to complete whole procedure while the Tessier sequential extraction method took around 12 hours. Ultrasound-sonication method was estimated as one of the best methods with a relatively short application time and no requirement of high temperature treatment.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 2006.11a
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• pp.167-170
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• 2006

본 논문은 선형분류기인 LDA 융합모델과 최소거리패턴분류법을 이용한 얼굴표정인식 알고리즘 연구에 관한 것이다. 제안된 알고리즘은 얼굴 표정을 인식하기 위해 두 단계의 특징 추출과정과 인식단계를 거치게 된다. 먼저 특징추출 단계에서는 얼굴 표정이 담긴 영상을 PCA를 이용해 고차원에서 저차원의 공간으로 변환한 후, LDA 이용해 특징벡터를 클래스 별로 나누어 분류한다. 다음 단계로 LDA융합모델을 통해 계산된 특징벡터에 최소거리패턴분류법을 적용함으로서 얼굴 표정을 인식한다. 제안된 알고리즘은 6가지 기본 감정(기쁨, 화남, 놀람, 공포, 슬픔, 혐오)으로 구성된 데이터베이스를 이용해 실험한 결과, 기존알고리즘에 비해 향상된 인식률과 특정 표정에 관계없이 고른 인식률을 보임을 확인하였다.

• Ocean and Polar Research
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• v.26 no.2
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• pp.231-243
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• 2004

Sequential extraction was carried out on twenty two subsamples of three ferromanganese crusts from three seamounts (Lemkein, Lomilik, and Litakpooki) near the Marshall Islands in the western Pacific. The extraction was designed to fractionate Fe-Mn crust forming elements into low defined groups: (1) exchangeable and carbornate, (2) Mn-oxide, (3) Fe-oxyhyd.oxide, and (4) residual fraction. X-ray diffraction result shows that target material were well removed by each extraction step except for CFA in phosphatized crusts generation. According to chemical analysis of each leachate, most of elements in the Fe-Mn crusts are bound with two major phases. Mn, Ba, Co, Ni, Zn, (Fe, Sr, Cu, and V) are strongly bounded with Mn-oxide $({\delta}-MnO_2)$ phase, whereas Fe, Ti, Zr, Mo, Pb, Al, Cu,(V, P, and Zn) show chemical affinity with Fe-oxyhydroxide phase. This result indicates that significant amount of Al, Ti, and Zr can not be explained by detrital origin. Ca, Mg, K, and Sr mainly occur as exchangeable elements and/or carbonate phase. Outermost layer 1 and inner layer 2 which are both young crusts generations are similar in chemical speciations of elements. However, some of Fe-oxyhydroxide bounded elements (Pb, Y, Mo, Ba, Al, and V) in phosphatized innermost layer 3 are released during phosphatization and incorporated into phosphate (Pb, Y, Mo, and Ba) or Mn-oxide phase (Al and V). Our sequential extraction results reveal that chemical speciations of elements in the hydrogenetic crusts are more or less different from interelemental relationship calculated by statistical method based on bulk chemistry.

• Economic and Environmental Geology
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• v.37 no.1
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• pp.87-98
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• 2004

Tailings and contaminated soils from Cheongyang mine and Seobo mine have been analysed by ICP-AES from 5-step sequential extraction method of multielement determination on extraction solutions at each step. As and Co within tailings and contaminated soils from Cheongyang mine and Seobo mine are mainly in the residual phase. In case of Cd, Cu and Zn, the most dominant fraction for tailings of Cheongyang mine is the oxidizable phase, while tailings of Seobo mine is dominated by the residual phase. In contaminated soils from Seobo mine, the predominant fraction for Cd, Cu and Zn is the Fe-Mn oxide phase. The exchangeable fraction of Pb in tailings from Cheongyang mine and Seobo mine is relatively high compared with those of other metals; whereas Pb fraction in contaminated soils from Seobo mine is largely associated with the residual fraction.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.161-161
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• 2002

최근, 무분별한 농약사용으로 인해 조리 후에도 잔류하는 농약성분의 독성이 문제시 되고 있어 인체 내로의 섭취 전에 독성 유무와 그 정도를 확인할 수 있는 방법 개발을 위한 연구가 활발히 진행되고 있다. 본 연구에서는 채소류, 곡류, 과일류 등에 빈번히 사용되는 농약성분의 독성 확인과 함께 이들 식품 내에 잔류하는 농약성분의 검출을 위한 방법을 연구하였으며, 이들의 독성 유무를 유전자 재조합된 5종의 발광성 미생물을 이용하여 확인하였다. 또한 이들 농약류가 식품 내에 잔류하여 발생하는 독성을 분석하기 위해 식품내 잔류하는 농약 성분의 추출에 필요한 효율적인 추출 용매를 제작하였으며, 농약성분의 화학적 성질에 따라 추출 방법을 두 단계로 분리하여 추출률을 향상시켰다. 뿐만 아니라, 식품류의 특성에 적합하게 식품별로 특정한 추출방법을 연구하였으며, 추출된 농약 성분의 독성 및 안정성 역시 발광성 박테리아를 이용하여 확인하여, 전체적인 추출방법의 효율을 진단하였다.

• Parasites, Hosts and Diseases
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• v.27 no.1
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• pp.1-8
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• 1989

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the purity of antigens used. To solve this problem. many workers have tried to find species-specific components of antigens, The present study was performed to determine the antigenic profile of crude saline extracts of 3, 5, 8 and 12-week old p. westermani worms, which were collected from experimentally infected cats, based on SDS-PAGE and immunoblot technique. The results were as follows: 1. The SDS-PAGE showed at least 30 Protein bands ranging from 229 kDa to 10 kDa molecular weight. The protein components of p. wsstermani changed chronologically during its developmental period. The 229 kDa band was recognized only in 12-week old worms ($$SEP_{l2}$). 2. Analysis by ELISA showed a significant increase in antibody levels at 3 weeks in infected cats using crude saline extract antigens ($SEP_3,{\;}SEP_5,{\;}SEP_8,{\;}SEP_{l2}$). 3. By EITB using $SEP_3$ and $SEP_5$ infected cats recognisea major protein bands with molecular weight of 60, 35, 28, 25 or 21 kDa at 3~12 weeks of infection, and 3 additional antigens, 19, 13 and 10 kDa, were detected at 8~12 weeks of infections. 4. Using $SEP_8$ 5 antigens, 91, 85, 31, 25 and 21 kDa, were consistently detected by all infected sera tested. In addition, 3 antigens of lg. 13 and 10 kDa were detected at 8~12 weeks of infection. Using $SEP_12$, similar results were obtained with that by using $SEP_8$ and 1 additional antigen of 229 kDa, specifically reacting with the sera from 12 weeks of in(traction, was recognized.

• Journal of Convergence for Information Technology
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• v.11 no.2
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• pp.211-218
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• 2021

The purpose of this study is to develop how to extract essential oil from heartwood of chamaecyparis obtusa and commercialize eco-friendly products using essential oil. Manufacturing process consisted of 8 steps: pulverization, input, pretreatment, extraction(steam distillation), steam emission, cooling, separation, aging. Through the method developed in this study, chamaecyparis obtusa oil with excellent quality in antibacterial effect can be extracted with high productivity. Also, chamaecyparis obtusa spray using this oil had good antibacterial and deodorant effects, and got no irritation results in the clinical test. To commercialize spray, we built a commercial website and performed consumer survey on the site. The survey results showed that respondents had positive attitude toward the products and their messages in the site. When the production standardization through precise quality control and the optimization of composition ratio are accomplished, this study will contribute to commercialize various types of cosmetics and quasi-drugs.

• The Journal of the Korea Contents Association
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• v.12 no.7
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• pp.473-482
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• 2012

This study is aimed to design for qualification structure and criteria for preparation of qualification test of the Livestock. In this study, a DACUM(Development A curriculum) method job analysis to extract contents such as task range, eligibility, examination standards, selection criteria of examination questions, examination methods, etc was done by an job analysis committee composed of 7 specialists in the industry and academia. In the first place, this report presents job analysis models by procedures for developing selection criteria of examination questions of the Livestock. The first step is preparation for job analysis(data research, interviews and selection of experts), the second step the establishment of job models(first expert council and site study), the third step the drawn-out of knowledge, skills, and tools(the second expert council and site study), the fourth step the selection of courses and an examination subject(the third expert council), the fifth step the establishment of guidelines for making questions(the third site study).

• Journal of Korean Society of Environmental Engineers
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• v.27 no.1
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• pp.25-35
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• 2005

This study analyzed total concentrations and existing forms of heavy metals in soils of Ulsan using a sequential extraction method. Soil samples were collected from 6 categorized areas including green, residential, heavy traffic, petrochemical industrial complex(IC), mechanical and shipbuilding IC, and non-ferrous metal IC areas. which represent different emission characteristics. The highest total concentrations of heavy metals by a sequential extraction analysis were observed in the soils collected from the non-ferrous metal IC area, followed by the mechanical and shipbuilding IC and heavy traffic areas. Dominant(> 50%) existing forms of Cd, Cr and Ni were residual forms followed by Fe and Mn oxides in almost areas. Residual fractions in the non-ferrous metal IC areas were relatively lower than those in other areas. However, the fractions of organic and sulphides in the IC areas were higher. The dominant farms of Cu were much different with the investigated areas. In most areas, the dominant forms of Pb and Zn were Fe and Mn oxides, followed by residual fraction for Pb. The exchangeable and carbonate fractions represent mobility of metallic elements in soils. They are also significantly affected by the environmental renditions, such as pHs of soil and rainfall. In this study the exchangeable and carbonate fractions were lower than other fractions. Because the total concentrations of heavy metals in the soils of the non-ferrous metal IC area were extremely high, however, the mobile fractions of heavy metals in the IC area would be significant. Thus a large amount of heavy metals can be released into plants, water bodies, and soils. Therefore, urgent measures, such as source control for soil remediation of heavy metals, in the non-ferrous metal IC areas are essentially required. Analysis results obtained from the sequential extraction and the aqua regia extraction showed a high correlation, whose determination coefficients(R2) of heavy metals except Cd approximately ranged from 0.7 to 0.9.