• Title/Summary/Keyword: 단계별추출

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Effect of Angelica keiskei Extract on Apoptosis of MDA-MB-231 Human Breast Cancer Cells (신선초 추출물이 인체 유방암 세포 MDA-MB-231의 세포 사멸에 미치는 영향)

  • Jeong, Yu-Jin;Kang, Keum-Jee
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.12
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    • pp.1654-1661
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    • 2011
  • We investigated the effect of Angelica keiskei ethanol (AKE) extract on cell death in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured in the presence 125, 150 and 175 ${\mu}g$/mL concentrations of AKE for 24 hours. MTT assays demonstrated that mitochondrial dehydrogenase activities decreased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In contrast, the proportion of dual staining with Hoechst 33342/ethidium bromide(EtBr) for cell death increased in a dose-dependent manner in MDA-MB-231 cells (p<0.05). In particular, the levels of cell death caused by apoptotic program showed marked increases in the 150 and 175 ${\mu}g$/mL AKE groups, as revealed by flow cytometry. An apoptotic suppressor gene, Bcl-2, significantly decreased at the transcript level (p<0.05). The expression levels of proapoptotic genes, both Bax and caspase 3 significantly increased (p<0.05). Furthermore, the ratio of Bcl-2/Bax mRNA which is considered to be an important indicator of apoptosis, significantly decreased in a dose-dependent manner (p<0.05). These results taken together indicate that, the AKE extract used in this study induces cell death in MDA-MB-231 human breast cancer cells.

Study on the Introduction of Safety Management Level Evaluation System for Shipping Company by Imitation Strategy (모방전략을 이용한 해운선사 안전관리 수준 평가제도 도입방안 연구)

  • Kim, Hwa-Young
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.19 no.4
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    • pp.366-374
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    • 2013
  • Maritime transportation circumstance is changing rapidly in accordance with the increase of cargo volume between countries and increase of marine leisure activities by improvement of quality of life. And the circumstance for ship operation is also changing due to aging of seafarers and increasing of foreign seafarers. To cope with such changes in maritime environment well, it requires higher safety management skills from shipping companies, the main subject in charge of the safety matter. In this paper, we analyzed domestic and foreign similar system, and then applied imitation strategies for introduction of an unific evaluation and management system that was consist of marine accidents, port state control, ISM Code and so on from shipping companies. We defined that the imitation industry and system are converted accident ration of construction industry and traffic safety excellence company of road transportation, and then extracted relevant law, evaluation index, incentive system as a imitation subject. We also proposed scheme that introduction of basis law, and evaluation tool with marine accidents ratio, port state control & ism code result, and incentives such as immunity of safety inspection or reduction of commission for introduction of company's safety management level evaluation system. Finally, we proposed the imitation timing and plan in stages for system's sustainable development through the prompt introduction and continuous enforcement.

Implementation Method of GIS Map for 3D Liquefaction Risk Analysis (3차원 액상화 위험분석을 위한 GIS Map 구현 방안)

  • Lee, Woo-Sik;Jang, Yong Gu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.6
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    • pp.10-17
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    • 2020
  • Recently, the liquefaction phenomenon was first discovered in Korea due to a magnitude 5.4 earthquake that occurred in Pohang, Gyeonsangbuk-do. When liquefaction occurs, some of the water and sand are ejected to the ground, producing a space, which leads to various dangerous situations, such as ground subsidence, building collapse, and sinkhole generation. Recently, the necessity of producing a liquefaction risk map in Korea has increased to grasp potential liquefaction areas in advance. Therefore, this study examined the drilling information from the national geotechnical information DB center at the Ministry of Land, Infrastructure, and Transport to produce a liquefaction risk map, and developed a module to implement functions for basic data modeling and 3D analysis based on drilling information database extraction and information. Through this study, effective interlocking technology of the integrated database of national land information was obtained, and three-dimensional information was generated for each stage of liquefaction risk analysis, such as soil resistance value and a liquefaction risk map. In the future, the technology developed in this study can be used as a comprehensive decision support technology for establishing a foundation for building 3D liquefaction information and for establishing a response system of liquefaction.

Prediction of Wind Damage Risk based on Estimation of Probability Distribution of Daily Maximum Wind Speed (일 최대풍속의 추정확률분포에 의한 농작물 강풍 피해 위험도 판정 방법)

  • Kim, Soo-ock
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.19 no.3
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    • pp.130-139
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    • 2017
  • The crop damage caused by strong wind was predicted using the wind speed data available from Korean Meteorological Administration (KMA). Wind speed data measured at 19 automatic weather stations in 2012 were compared with wind data available from the KMA's digital forecast. Linear regression equations were derived using the maximum value of wind speed measurements for the three-hour period prior to a given hour and the digital forecasts at the three-hour interval. Estimates of daily maximum wind speed were obtained from the regression equation finding the greatest value among the maximum wind speed at the three-hour interval. The estimation error for the daily maximum wind speed was expressed using normal distribution and Weibull distribution probability density function. The daily maximum wind speed was compared with the critical wind speed that could cause crop damage to determine the level of stages for wind damage, e.g., "watch" or "warning." Spatial interpolation of the regression coefficient for the maximum wind speed, the standard deviation of the estimation error at the automated weather stations, the parameters of Weibull distribution was performed. These interpolated values at the four synoptic weather stations including Suncheon, Namwon, Imsil, and Jangsu were used to estimate the daily maximum wind speed in 2012. The wind damage risk was determined using the critical wind speed of 10m/s under the assumption that the fruit of a pear variety Mansamgil would begin to drop at 10 m/s. The results indicated that the Weibull distribution was more effective than the normal distribution for the estimation error probability distribution for assessing wind damage risk.

Characteristics of Edgetones by Jet-Cylinder Interaction (분류와 원통에 의해 발생하는 쐐기소리의 특성)

  • 한희갑;김승덕;안진우;권영필
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 1996.04a
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    • pp.235-239
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    • 1996
  • 분류가 모서리에 충돌할 때 발생하는 순음성 소리인 쐐기소리(edgetone)는 공력음향의 대표적인 현상으로서 지금까지 수많은 연구가 있어 왔으며 그 대부분의 특성이 규명되었다고 할 수 있다. 쐐기소리의 발생기구인 되먹임(feedback) 이론을 처음으로 제안한 이는 Powell로서 그는 되먹임사이클의 위상조건에 의하여 주파수특성에 관한 모델을 제안하였으며, 최근 그 모델의 위상인자에 관하여 Kwon은 새로운 값을 제안한 바 있다. 그런데, 쐐기소리의 이론은 주로 분류가 쐐기나 벽에 충돌할 경우에 집중되어 왔으며 분류가 원통에 충돌하여 발생하는 경우에 관한 연구는 Krothapalli의 초음속분류에 관한 연구와 Mochizuki등의 아음속분류에서 원통지름의 영향에 관한 연구를 들 수 있을 뿐이다. Mochizuki등은 원통의 지름이 노즐의 높이보다 작은 경우에 쐐기 소리의 주파수가 원통의 와류이탈(vortex shedding) 주파수와 같은 것을 관찰하였다. 그러나 분류와 원통이 작용하여 발생하는 쐐기소리의 주파수 특성에 관한 이론적 해석을 시도한 연구는 없으며 또한 방사음장의 특성에 관하여도 Han과 Kwon에 의한 모델이 발표된 바 있으나 실험적으로 입증되지 못하였다. 따라서, 본 연구의 목적은 2 fig.1과 같이 2차원 분류가 원통에 충돌할 때 발생하는 쐐기소리의 주파수특성의 정량적인 모델을 세우고 방사음장의 지향특성의 이론 모델을 확립하는 것이다. 먼저 주파수특성을 실험하고 되먹임이론을 적용하여 분석하므로써 유효음원의 위치를 구하고 또한, 수직벽에 작용하여 발생하는 충돌음(impinging tone)의 경우를 실험하여 주파수특성을 비교 고찰하므로써 유효음원의 위치에 관한 이론을 입증한다. 아울러 원통과 평면벽의 각 경우에 방사음장의 지향특성을 측정하고 고찰한다.2,5,6]을 단계별로 고찰하여, 점점 까다로워져 가는 선박 진동규제[3,4]에 대처하고 승무원의 안락성에 대한 욕구, 구조물의 안전성, 장비의 성능보존이 만족되는 저진동 선박의 건조를 위해 향후 해결해야할 과제들을 도출하여 선박진동분야이 연구개발 방향을 제시하고자 한다. 하는 것은 진단의 정밀도에 문제가 있을 것으로 생각된다. 따라서 언어적진리치가 도입되어 [상당히 확실], [확실], [약간 확실] 등의 언어적인 표현을 이용하여 애매성을 표현하게 되었다. 본 논문에서는 간이진단 결과로부터 추출된 애매한 진단결과중에서 가장 가능성이 높은 이상원인을 복수로 선정하고, 여러 종류의 수치화할 수 없는 언어적(linguistic)인 정보ㄷㄹ을 if-then 형식의 퍼지추론으로 종합하는 회전기계의 이상진단을 위한 정밀진단 알고리즘을 제안하고 그 유용성을 검토한다. 존재하여도 모우드 변수들을 항상 정확하게 구할 수 있으며, 또한 알고리즘의 안정성이 보장된 것이다.. 여기서는 실험실 수준의 평 판모델을 제작하고 실제 현장에서 이루어질 수 있는 진동제어 구조물에 대 한 동적실험 및 FRS를 수행하는 과정과 동일하게 따름으로써 실제 발생할 수 있는 오차나 error를 실험실내의 차원에서 파악하여 진동원을 있는 구조 물에 대한 진동제어기술을 보유하고자 한다. 이용한 해마의 부피측정은 해마경화증 환자의 진단에 있어 육안적인 MR 진단이 어려운 제한된 경우에만 실제적 도움을 줄 수 있는 보조적인 방법으로 생각된다.ofile whereas relaxivity at high field is not affected by τS. On the other hand, the change in τV does not affect low field profile but strongly in fluences on both

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Remote Sensing을 이용한 태화강 하구 수심정보 획득 - Landsat 7 ETM 다중분광영상을 사용

  • Oh, Chang-Seok;Cho, Hong-Je;Song, Yeong-Min
    • Proceedings of the Korea Water Resources Association Conference
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    • 2006.05a
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    • pp.1530-1534
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    • 2006
  • 원격탐사 기법을 이용한 수심측정은 하나 혹은 그 이상의 파장대에서 수심과 반사되는 에너지 사이의 관계를 찾아내는데 달려 있다. 수심 정보를 획득하기 위한 스펙트럼의 최적 파장길이는 다중분광영상(Landsat 7 ETM)의 blue band에 해당하는 약 $0.48{\mu}m$이며, 이 band를 이용하여 연안의 수심을 측량하기도 한다. 하지만 단일밴드에 의해서 측정된 값을 이용한 수심측정은 해저표면에 의한 반사에 심각한 영향을 받을 수 있기 때문에 신뢰할 만한 결과를 얻을 수 없다. 따라서 본 연구에서는 해수와 관련한 여러 가지 변수들을 결정하기 위하여 다량의 실측 데이터를 필요로 하지 않는 선형다중밴드방식을 이용하여 2개의 Landsat 영상으로 태화강 하구의 수심정보를 추출하고 태화강 본류에 대한 수심정보획득과 하상변동에 대한 분석 가능성을 파악하였다. 그 결과 임의로 선정한 표본 50개 지점에 대한 영상분석에 의한 수심값과 해도의 수심값의 잔차 평균이 각각 2.29m, 2.43m로 비교적 큰 잔차를 보였다. 하지만 20m 미만의 수심대의 표본만을 확인한 결과 각각 1.73m, 1.88m로 잔차 평균이 크게 감소하였다. 2000년, 2003년 영상을 비교한 결과, 1번 2번 3번 지역에서 평균적으로 약 1.838m정도 2003년 수심이 감소한 것으로 나타났다. 본 연구에서 20m 미만의 수심 측량은 낮은 해상도의 위성영상이라도 실제 수심과 근접하고 있는 것으로 판단 할 수 있었다. 이것으로 넓은 지역을 경제적으로 수심자료를 획득할 수 있는 위성영상분석을 이용한 수심측정은 활용성이 있는 것으로 나타났다. 하지만 해저표면의 형태와 해수면의 상태 등 수심측정에 미치는 영향에 관한 실측데이터에 대한 자료수집과 분석이 선행된다면 더욱 좋은 결과를 도출할 수 있을 것으로 판단된다.A}$는 최대암모니아 섭취률을 이용하여 구한 결과 $0.65d^{-1}$로 나타났다.EX>$60%{\sim}87%$가 수심 10m 이내에 분포하였고, 녹조강과 남조강이 우점하는 하절기에는 5m 이내에 주로 분포하였다. 취수탑 지점의 수심이 연중 $25{\sim}35m$를 유지하는 H호의 경우 간헐식 폭기장치를 가동하는 기간은 물론 그 외 기간에도 취수구의 심도를 표층 10m 이하로 유지 할 경우 전체 조류 유입량을 60% 이상 저감할 수 있을 것으로 조사되었다.심볼 및 색채 디자인 등의 작업이 수반되어야 하며, 이들을 고려한 인터넷용 GIS기본도를 신규 제작한다. 상습침수지구와 관련된 각종 GIS데이타와 각 기관이 보유하고 있는 공공정보 가운데 공간정보와 연계되어야 하는 자료를 인터넷 GIS를 이용하여 효율적으로 관리하기 위해서는 단계별 구축전략이 필요하다. 따라서 본 논문에서는 인터넷 GIS를 이용하여 상습침수구역관련 정보를 검색, 처리 및 분석할 수 있는 상습침수 구역 종합정보화 시스템을 구축토록 하였다.N, 항목에서 보 상류가 높게 나타났으나, 철거되지 않은 검전보나 안양대교보에 비해 그 차이가 크지 않은 것으로 나타났다.의 기상변화가 자발성 기흉 발생에 영향을 미친다고 추론할 수 있었다. 향후 본 연구에서 추론된 기상변화와 기흉 발생과의 인과관계를 확인하고 좀 더 구체화하기 위한 연구가 필요할 것이다.게 이루어질 수 있을 것으로 기대된다.는 초과수익률이 상승하지만, 이후로는 감소하므로, 반전거래전략을 활용하는 경우 주식투자기간은 24개월이하의 중단기가 적합함을 발견하였다. 이상의 행태적 측면과 투자성과측면의 실증결과를 통하여 한국주식시장에 있어서 시장수익률을 평균적으로 초과할 수 있는 거래전략은 존재하므로 이러한 전략을 개발 및 활용할 수

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Simultaneous Determination of Pesticide Residues in Soils by Dichloromethane Partition - Adsorption Chromatography - GC-ECD/NPD Analytical Methods (Dichloromethane 분배 - 흡착 크로마토그래피 - GC-ECD/NPD 분석법에 의한 토양잔류농약 다성분 분석)

  • Kim, Chan-Sub;Lee, Byung-Moo;Park, Kyung-Hun;Park, Byung-Jun;Park, Jae-Eup;Lee, Young-Deuk
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.361-370
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    • 2010
  • Considering the efficiencies of the preparation process at each stage obtained in previous studies, the analytical determination method was established for multi-pesticide residues in soils. It consist of the acetone-extraction, the dichloromethane-partition, the Florisil or silica-gel chromatography and the gas chromatography analysis equipped with the electron capture detector and the nitrogen-phosphorus detector. In the soil recovery test by Florisil clean-up system, the number of pesticides recovered in the range of 70~120% and showed less than 20% of RSD were 165 pesticides for paddy soil, 169 pesticides for upland soil and 159 pesticides in both soils through the tested 183 pesticides. And in the soil recovery test by silica-gel system, the number of pesticides recovered in the range of 70~120% and showed less than 20% of RSD were 154 pesticides for paddy soil, 145 pesticides for upland soil, and 134 pesticides in both soils.

Relationship among User's Security Need Sufficiency, Customer Satisfaction and Life Satisfaction in Electronic Security System (기계경비시스템 이용자의 안전욕구충족과 이용만족 및 생활만족의 관계)

  • Kim, Chan-Sun
    • The Journal of the Korea Contents Association
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    • v.9 no.7
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    • pp.257-267
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    • 2009
  • This study aims at diagnosing the relationship among user's security need Sufficiency, customer satisfaction and life satisfaction in electronic security system. For the achievement of this study selected electronic security system users in Seoul as a population for about 25 days from June 20$^{th}$, 2008 to July 15$^{th}$, 2008, segmented. This study selected 378 peoples by distributing 400 unities in total for each 80 peoples throughout purposive sampling method. The final 302 samples were used in statistics. Collected data was analyzed based on the aim of this study using SPSSWIN 16.0, and factor analysis, reliability analysis, stepwise multiple regression analysis and path analysis were used as statistic techniques to analyze. The conclusions are the followings; First, The higher bodily, environmental, mental, informational, and physical security need the more body and property protection satisfaction and facility customer satisfaction. The higher bodily, environmental, and mental security need the more employee service satisfaction. Second, The higher bodily, environmental, informational, and physical security need are perceived, the more influence is marked with life satisfaction and security life satisfaction. Third, The higher personal and property protection, facility, and employee service satisfaction the more security life satisfaction. Also, the higher customer service and personal and property protection satisfaction are perceived, the more influence is marked with life satisfaction. Fourth, Security need sufficiency has little influence on life satisfaction directly, but it has high influences on life satisfaction through customer satisfaction of electronic security system.

A Pilot Study for Development of the Serious Game Contents for Education in the Elderly Diabetes (노인 당뇨환자 교육용 기능성 게임 콘텐츠 개발을 위한 예비연구)

  • Kim, Yu Jeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.7
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    • pp.184-192
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    • 2017
  • This study was a pilot test of serious educational game content(named Roly Poly 160) designed to enhance self-care by elderly diabetics. Roly Poly 160 was developed in eight steps (literature review, demand survey and consultation, extraction of serious content using games, development of Roly Poly 160, pretest for users, workshop for health care workers, final completion of Roly Poly 160 and user satisfaction survey of Roly Poly 160). Roly Poly 160 is intuitively structured in three modules (self-management, card games, and quiz games) that can be self-managed by the elderly and is designed based on six principles. First, we constructed an intuitive interface considering the age of users. Second, the menu was selected as the main menu for Koreans and calorie learning was made at the same time. Third, the calories and nutrients (carbohydrate, fat, protein, calcium, sodium) of selected foods are analyzed, and all test data recorded in the questionnaire chart are graphically displayed by year, month and week so that the change trends can be grasped at a glance. Fourth, necessary data were saved and output and used as educational data. Fifth, user data are made compatible and aggregated and up to 100 million members can be registered. Sixth, it is designed to be developed as a mobile app if necessary. Using Roly Poly 160, 119 diabetic patients were diagnosed with diabetes, and the satisfaction score was 4.26 out of 5. These findings indicate that Roly Poly 160 is appropriate as a diabetes self-care tool and suggests that there is a possibility to use it as a program to educate people about diabetes in public health centers, hospitals and clinics.

Characteristic Analysis of Major Issues in Negotiating Concession Agreement for BTO Projects Using AHP (AHP 기법을 이용한 BTO 민간투자사업 협상의 주요쟁점 특성분석)

  • Roh, Tae-Ho
    • The Journal of the Korea Contents Association
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    • v.7 no.8
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    • pp.234-244
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    • 2007
  • Both competent authorities and private entrepreneurs face plenty of risks when negotiating BTO(Build Transfer Operation) methods of PPI(Private Participations in Infrastructures) projects. Also, success and failure of projects largely depend on the concession agreement contract. In this study, for more efficient negotiation, major issues are examined, and quantitatively analyzed to find out not only the relationship but also the characteristics of which these issues share. Questionnaire research was conducted through both research and by experts who were divided into an ordering agency, design company and constructor. Characteristics of major issues were quantitatively analyzed using the Likert index method, ANOVA(ANalysis of VAriance) and AHP(Analytic Hierarchy Process) analysis. Case studies were examined in order to estimate construction cost, rates of return, government finance support, level of operation-revenues guarantees, estimating operational costs and usage fees. With these 6 items, relative priority, relative impression which is sensible to risk and extent of difficulty in presenting data which is objective were quantitatively analyzed by the ordering agency, design company and constructor groups. From the analysis, there were some similarities between the design company and constructor groups while there was less of similarities between the ordering agency and design company or constructor. The government is diversifying the methods of project promotion, and PPI project from infrastructure-centered to public culture facilities are being expand. The current study will provide not only supporting efficient negotiation but also revitalizing PPI projects.