• Title/Summary/Keyword: 다중 유전체

Search Result 74, Processing Time 0.026 seconds

Comparison of the complete chloroplast genome sequence of Solanum stoloniferum with other Solanum species generates PCR-based markers specific for Solanum stoloniferum (엽록체 전장유전체 정보를 이용한 감자 야생종 Solanum stoloniferum 구별 분자 마커 개발)

  • Kim, Soojung;Park, Tae-Ho
    • Journal of Plant Biotechnology
    • /
    • v.47 no.2
    • /
    • pp.131-140
    • /
    • 2020
  • Solanum stoloniferum, one of the wild tetraploid Solanum species belonging to the Solanaceae family, is an excellent resource for potato breeding owing to its resistance to several important pathogens. However, the sexual hybridization of S. stoloniferum with S. tuberosum (potato) is hampered due to the sexual incompatibility between the two species. To overcome this and introgress the various novel traits of S. stoloniferum in cultivated potatoes, cell fusion can be performed. The identification of the fusion products is crucial and can be achieved with the aid of molecular markers. In this study, the chloroplast genome sequence of S. stoloniferum was obtained by next-generation sequencing technology, and compared with that of six other Solanum species to identify S. stoloniferum-specific molecular markers. The length of the complete chloroplast genome of S. stoloniferum was found to be 155,567 bp. The structural organization of the chloroplast genome of S. stoloniferum was similar to that of the six other Solanum species studied. Phylogenetic analysis of S. stoloniferum with nine other Solanaceae family members revealed that S. stoloniferum was most closely related to S. berthaultii. Additional comparison of the complete chloroplast genome sequence of S. stoloniferum with that of five Solanum species revealed the presence of six InDels and 39 SNPs specific to S. stoloniferum. Based on these InDels and SNPs, four PCR-based markers were developed to differentiate S. stoloniferum from other Solanum species. These markers will facilitate the selection of fusion products and accelerate potato breeding using S. stoloniferum.

Nano-scale Power Splitters by using Plasmonic Multimode Interference Couplers (플라즈마 다중모드 간섭 결합기를 사용한 나노 크기의 전력분배기)

  • Ho, Kwang-Chun
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.11 no.4
    • /
    • pp.47-52
    • /
    • 2011
  • Nano-scale power splitter based on Si plasmonic waveguides are designed by utilizing the multimode interference (MMI) coupler. Effective dielectric method and longitudinal modal transmission-line theory are used for simulating the light propagation and optimizing the structural parameters at 3-D guiding geometry. The designed $1{\times}2$ 50:50 MMI power splitter has a nano-scale size of only $800nm{\times}850nm$. In order to achieve a variable power splitting ratio, a $2{\times}2$ MMI coupler is designed and the corresponding power splitting ratio can be tuned in the range of 78.5%:15.5%~5.5%:86.6%. Also, it is shown that it has a large bandwidth of $1.5{\mu}m{\sim}1.7{\mu}m$. In this range, the transmission is over 0.8.

Design of a Simultaneously Operable Tri-band Antenna for SATCOM (위성통신용 삼중대역 동시사용 안테나 설계)

  • Woo, Byung-Seok
    • The Journal of the Institute of Internet, Broadcasting and Communication
    • /
    • v.15 no.2
    • /
    • pp.195-202
    • /
    • 2015
  • As satellite communications services are showing gradual growth, so are the needs for dual or tri-band antennas greater than performance of conventional single-band antennas. In this paper, a simultaneously operable tri-band antenna for SATCOM(Satellite Communications) is proposed. It is different from conventional methods of implementing tri-band through replaceable horns, while it is designed with a tri-band feed assembly composed of corrugated and dielectric horns and reflectors of gregorian type to transmit and receive tri-band of X, Ku and Ka band simultaneously. And simulated and measured values were compared and analyzed for characteristics of the proposed antenna. In the results, radiation patterns of the proposed tri-band antenna were close to the simulated ones and also met specifications.

Paratic Impedance Extraction of FC-PGA Package Pin using the Static Fast Multipole Method (Static FMM을 이용한 FC-PGA 패키지 핀에서의 기생 임피던스 추출)

  • 천정남;이정태;어수지;김형동
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.12 no.7
    • /
    • pp.1076-1085
    • /
    • 2001
  • In this paper, the FMM(Fast Multipole Method) combined with GMRES(Generalized Minimal RESidual Method) matrix solver is used to extract the parasitic impedance for complicated 3-D structures in uniform dielectric materials which limit the use of MoM(Method of Moment) due to its large computation time and memory requirement. This algorithm is a fast multipole-accelerated method based on quasistatic analysis and is very efficient for computing impedance between conductors. This paper proved the accuracy and efficiency of the FMM by comparing with MoM in simple examples. Finally the parasitic impedance of FC-PGA(Flip Chip Pin Grid Array) Package pins has been extracted by this algorithm and we have considered the possibility of the EMI/EMC problem caused by the signal interference.

  • PDF

An XML Data Management System and Its Application to Genome Databases (XML 데이타 관리시스템과 유전체 데이타베이스에의 응용)

  • 이경희;김태경;김선신;이충세;조완섭
    • Journal of KIISE:Databases
    • /
    • v.31 no.4
    • /
    • pp.432-443
    • /
    • 2004
  • As the XML data has been widely used in the Internet, it is necessary to store and retrieve the XML data by using DBMSs. However, relational DBMSs suffer from the model difference between graph structure of the XML data and table forms in relational databases. We propose an ORDBMS-based DTD-dependent XML data management system Xing. Xing stores XML data in a DTD-dependent form in an object database. Since the object database schema has a graph structure and supports multi-valued attributes, mapping from an XML data model and queries into an object data model and OQLs is a simple problem. For rapid storing of large quantities of the XML data, we use SAX parser with customized Xing-tree which requires a small memory space compared with the DOM-tree. Xing also returns the query result in an XML document form. We have implemented the Xing system on top of UniSQL object-relational DBMS for the validity checking and performance comparison. For XML genome data from GenBank, and experimental evaluation shows that Xing can provide significant performance improvement (maximum 10 times) compared with the relational approach.

Research on Passive Optical Devices Using Silicon on Insulator (Silicon on Insulator 수동광소자에 관한 연구)

  • 박종대
    • Proceedings of the Optical Society of Korea Conference
    • /
    • 2000.08a
    • /
    • pp.156-157
    • /
    • 2000
  • 낮은 도핑의 silicon은 광통신에서 사용하는 1.3 및 1.55 um의 광파장 영역에서 0.01dB/cm 이하의 낮은 흡수 손실을 가짐으로 인해, core 층으로 silicon을 사용하며 상부 및 하부 cladding 층으로 SiO$_2$와 같은 유전체 박막 구조를 갖는 SOI (Silicon on Insulator)를 사용한 수동광소자에 대한 연구가 1990년대부터 진행되고 있다. 또한 silicon의 특성상 SOI는strain에 의한 영향이 낮고, 광학적 비등방성이 적어서 polymer 및 silica를 이용한 광소자에 비해 편광의존도가 낮은 광소자를 구현할 수 있는 장점이 있다. 현재까지 연구되어온 SOI 수동광소자의 연구결과는 TE/TM 편광차에 따른 채널분리도가 약 0.04nm, 누화특성이 23dB 인 8채널 AWG의 연구결과가 있었으며, 스위칭시간 < 1msec, 소광비 17 dB의 광결합기와 마하젠더가 혼합된 광변조기 및 Bookham사에서 개발한 RX/TX 양방향 송수신 광모듈에 적용된 1.3/l.55 um 파장선택적 분리기, Silicon-CMOS 증폭기와 집적화된 4channel 광다중 수신기등에 대한 연구결과가 있었다. (중략)

  • PDF

PCR-based markers for discriminating Solanum demissum were developed by comparison of complete chloroplast genome sequences of Solanum species (가지속 식물의 엽록체 전장유전체 비교를 통한 PCR 기반의 Solanum demissum 특이적 분자마커 개발)

  • Park, Tae-Ho
    • Journal of Plant Biotechnology
    • /
    • v.48 no.1
    • /
    • pp.18-25
    • /
    • 2021
  • Solanum demissum is one of the wild Solanum species originating from Mexico. It has wildly been used for potato breeding due to its resistance to Phytophthora infestans. S. demissum has an EBN value of four, which is same as that of S. tuberosum, so that it is directly crossable for breeding purposes with the cultivated tetraploid potato (S. tuberosum). In this study, the chloroplast genome sequence of S. demissum obtained by next-generation sequencing technology was described and compared with those of seven other Solanum species to develop S. demissum-specific markers. Thetotal sequence length of the chloroplast genome is 155,558 bp, and its structural organization is similar to those of other Solanum species. Phylogenetic analysis with ten other Solanaceae species revealed that S. demissum is most closely grouped with S. hougasii and S. stoloniferum followed by S. berthaultii and S. tuberosum. Additional comparison of the chloroplast genome sequence with those of seven other Solanum species revealed two InDels specific to S. demissum. Based on these InDels, two PCR-based markers for discriminating S. demissum from other Solanum species were developed. The results obtained in this study will provide an opportunity to investigate more detailed evolutionary and breeding aspects in Solanum species.

Assessment of the Electromagnetic Pulse Shield Effectiveness of the Wave-guided Below Cutoff Filled with Water and Oil for Guaranteeing the Operational Sustainment of the Command Post (지휘소 작전지속성 보장을 위한 도파관의 전자기파 차폐성능 향상방안)

  • Yoon, Sangho;Son, Kiyoung;Kim, Suk Bong;Park, Young Jun
    • Journal of the Korea Institute of Building Construction
    • /
    • v.13 no.6
    • /
    • pp.579-584
    • /
    • 2013
  • The stable fueling and water supply should be prerequisites to guarantee the operational sustainment of military command post. Meanwhile, in terms of the operational sustainment, it is verified that the current wave-guided below cutoff (WBC) system cannot satisfy the requirement of control associated with water supply and fueling within the command post. In this study, as the dielectric substance can block electromagnetic pulse (EMP), it was tried to identify the shielding effectiveness of the multi WBCs filled with water and diesel for attenuating the EMP effect using experiment based on the MIL STD 188-125-1. According to the experimental results, used water in the experiment show the shielding effectiveness from around 640 MHz frequency because of minerals contained in the water. Furthermore, it was noted that EMP attenuating strength of the WBC filled with diesel was enlarged from around 1,680 MHz frequency. Resultingly, it could be concluded that it is enough to supply stable water and diesel through the multi WBC to block EMP within the military command post for guaranteeing the military operations sustainment.

Simultaneous Detection of Cytomegalovirus, Epstein-Barr Virus, Hepatitis B Virus, and Parvovirus by a Multiplex PCR (다중 중합효소 연쇄반응을 이용한 DNA 바이러스의 동시검출)

  • Sung, Hye-Ran;Joo, Jin-Young;Lee, Chong-Kil;Chung, Yeon-Bok;Song, Suk-Gil
    • Korean Journal of Microbiology
    • /
    • v.43 no.1
    • /
    • pp.1-6
    • /
    • 2007
  • We describe a multiplex PCR method that can detect and differentiate simultaneously four different kinds of DNA viruses, Epstein-Barr virus (EBV), cytomegalovirus (CMV), hepatitis B virus (HBV) and parvovirus B19 (B19). Primers for the multiplex PCR reaction were designed to amplify specific regions of the EBV (pol), CMV (pol), HBV (pol) and B19 (ns) viral genomes and used to simultaneously detect individual viruses. In order to achieve optimal sensitivity and specificity for multiplex PCR, the thermo-cycling parameters, primer sequences, and concentration of each reaction components were optimized systematically. The sensitivity of the detection method ranged between 5 and 10 copies of viral genome with a mixture of multiple primer pairs. Furthermore, this highly sensitive test showed no cross-reactivity among the four viruses. Thus, the results obtained in this study provide evidence that the assay system is a good tool for supporting the diagnosis of viral infection and contamination.

A Design of the Multiband Small Chip Antenna Using the Branch Structure and Gap Feeding for Mobile Phone (가지 구조와 간극 급전을 사용한 휴대 단말기용 소형 유전체 다중 대역 칩 안테나)

  • Kim, Min-Chan;Kim, Hyung-Hoon;Park, Jong-Il;Kim, Hyeong-Dong
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
    • /
    • v.18 no.3 s.118
    • /
    • pp.298-304
    • /
    • 2007
  • In this paper, the antenna which has a multiband operation (GSM850, EGSM, DCS1800, USPCS, W-CDMA) is proposed. This antenna was designed by the commercial software HFSS 3-D EM simulator, and it is organized by using a meander branch structure which has a via and lines on FR-4$(\varepsilon_r=4.4)$ substrate. Especially, it has a gap feeding structure which makes good operation at overall bandwidth. The designed antenna is manufactured by PCB processing, and measured by using a network analyzer and a test chamber. The manufactured antenna with the dimension of 8 mm width, 20 mm height and 3.2 mm thickness is able to applied as an internal antenna for multiband mobile phones.