• Journal of Life Science
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• v.21 no.1
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• pp.81-88
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• 2011

Biological activities (${\alpha},{\alpha}'$-diphenyl-${\beta}$-picrylhydrazyl (DPPH) free radical scavenging activity, fibrinolytic activity and reducing power) and biochemical properties (protein content and electrophoretical protein patterns) were examined in solid state fermentation with Bacillus subtilis and Aspergillus kawachii using silkworm powder (SP) as substrate. The highest protein contents and free radical scavenging activities were seen in the SP fermented for 12 days with B. subtilis and A. kawachii, and these were in a time-dependent manner. The highest reducing power was seen in the SP fermented for 6 days with B. subtilis and for 12 days with A. kawachii, respectively. The highest fibrinolytic activities were seen in silkworm fermented for 6 days with B. subtilis and A. kawachii, but this activity was higher in the A. kawachii fermented SP than that of B. subtilis. When total protein patterns were analyzed by SDS-polyacrylamide gel electrophoresis (PAGE), the proteins of the SP fermented with B. subtilis for 3 days were completely degraded, while the protein degradation in the SP fermented with A. kawachii occurred after 12 days and this degradation increased proportionally to culture time. As a result, the SP fermented with both B. subtilis and A. kawachii showed higher fibrinolytic activities after 6 days of fermentation and antioxidative activity after 12 days, indicating that physiological activities of the fermented SP using these strains were highly improved compared to the unfermented SP, and that this compound could be a candidate material as a dietary supplement of healthy functional foods.

• Korean journal of applied entomology
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• v.29 no.3
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• pp.157-164
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• 1990

The surface patterns and the structures of transverse section of the egg-shell of the sikmoth, Bombyx mandarina, have been described by scanning electron microscope. Three spatially differentiated cross section, called lamellar, conic pillar and cover layers, are found on the mature eg-shell. Silkmoth chorion proteins were detected more than 80 components from a single chorion by two-dimensional electrophoresis. Major protein components of the egg-shell have bee identified on the basis of their isoelectric points and molecular weights, pH 4-6 and 6-30 kd. Several protein components are found entirely or predominantly in th cover layers.

• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.238-245
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• 2007

This survey was performed to elucidate whether pesticide can cause the non-spinning syndrome of silkworm, Bombyx mori in Yecheon and Sangju 2002. We primarily focused to Insect Growth Regulator Pesticides(IGR) used in that areas. In the result of the survey to the retail pesticide store at Yecheon and Sangju, seven pesticides of the total 12 IGR pesticides sold were used during spring sericuitural season. 7 pesticides might effect on silkworm in consideration of possibility of exposure according to the characteristics of used amount, spray drift, volatility and treatment time. Five chitin synthesis inhibitor involving diflubenzuron of 7 IGR pesticides were known as very toxic to silkwork after exposure by skin and feeding but no non-spinning syndrome was observed from several papers. Methoxyfenozide and tebufenozide also has used at sericultural season, but are ecdysone agonist. One of the most feasible suspect of non IGR pesticide, was molinate in consideration of its volatility property and wide spread use in the sericultural area. But it's symptom after exposure of molinate was not the same as the non-spinning syndrome broke out at the sericultural farm in Yecheon and Sangju.

• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.04a
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• pp.50-50
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• 2003

최근 누에동충하초 재배농가에서 대량발생한 오염균 중 일종은 누에치사 및 부정형 자실체를 발생시켜 생산성 감소시키고 상품성을 저하시켜 농가에 많은 피해를 가져오므로, 이상누에로부터 균을 분리하고 재접종 시험한 결과 원인균은 Paecilomyces farinosus(Holm ex S.F. Gray) Brown ＆ Smith로 동정되었으며 특성은 다음과 같다. (중략)

• Journal of Sericultural and Entomological Science
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• v.37 no.1
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• pp.16-26
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• 1995

DNA restriction fragment length polymorphisms(RFLPs) were used for the classification of 22 leading silkworm races and wild silkworm, Bombyx mandarina. A genomic DNA library from silkworm was partially constructed and was prescreened to evaluate the selected DNA probes. Three DNA probes (SP1-13, SP1-28, 10-42) were selected to determine the polymorphism between silkworm races. As a result, high polymorphism with the probe SP1-28, moderate polymorphism with SP1-13 and monomorphism with 10-42 were obse-rbed.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.151-154
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• 2000

The effect of silkworm hemolymph on insect cell apoptosis was investigated. The addition of silkworm hemolymph into the culture medium either before or during the baculovirus infection increased the host cell longevity. Silkworm hemolymph also inhibits apoptosis induced by actinomycin D, the RNA synthesis inhibitor. In this study, a flow cytometry was used for the quantitative analysis of apoptosis inhibition by silkworm hemolymph.

• Journal of Sericultural and Entomological Science
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• v.14 no.2
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• pp.73-79
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• 1972

This study was carried out to investigate the effects of the mulberry leaves sprayed with filtered solutions and non-filtered solutions of ginseng, on the various practical characteristics of the silkworm (Bombyx mori) in spring, summer and late autumn in 1971, and spring and autumn in 1972. 1. There was no evidence that the various ginseng concentrations influence the practical characteristics in the silkworms, according to the different instars and rearing seasons. 2. Non-filtered solutions of ginseng have a bad effect upon the silkworm characteristics, especially in pupation ratio and reelable cocoon ratio.

• Journal of Sericultural and Entomological Science
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• v.34 no.1
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• pp.15-20
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• 1992

Effects of starvation, ligation, refeeding and methoprene treatment in the feeding phase of the fifth instar larvae of Bombyx mori on the regulation of juvenile hormone esterase(JHE) activity were investigated. Starvation and ligation contributed to the reduction of JHE activity, however, JHE levels in starved larvae were slightly higher than in legated larvae. Haemolymph JEH activity of starved larvae was increased by refeeding, and duration of increasing time of JHE activity after starvation was related to duration of starvation. When starved larvae were applied methoprene topically, JHE activity were not changed at day 0 and 1, but were increased by 1.3-1.4 times between day 2 and 5. When ligated larvae were applied methoprene topically, JHE activity was not changed at day 0, but were increased by 1.9-2.3 times between day 1 and 5. These results suggest that head factor, juvenile hormone(JH) and nutrient are major factors in the regulation of JHE in the feeding phase of the fifth instar larvae of Bombyx mori. Especially JHE might be regulated by the co-operative action of head factor and JH. However, head factor plays important role in the early stage, while JH plays important role thereafter.

• Journal of Sericultural and Entomological Science
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• v.28 no.2
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• pp.35-37
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• 1986

To reduce the number of rearing season required for preservation of multivoltine silkworms which do not produce diapause eggs, the optimal egg stage, temperature, and period of cold storage were examinede using hatchability as an indicator of viability. Multivoltine silkworm starains MR, SPT, and HM were used in the study. 1. The hatchability of multivoltine silkworm eggs (MR and STP) preserve at 5$^{\circ}C$ for 30 days was 80% for the eggs chilled from 2 days after oviposition but less 5% for those chilled from 7 days after ovipostion. 2. When 2 day-old eggs of multivoltine silkworm (HM) were preserved between -2.5$^{\circ}C$ to 7.5$^{\circ}C$ for 15 to 60 days, $0^{\circ}C$ and 2.5$^{\circ}C$ showed the highest hatchability with 91% at 30 days and 61% at 60 days storage, respectively. 3. From these results, it can be concluded that by preserving 2 day-old eggs at 2.5$^{\circ}C$ for 50 to 60 days, rearing seasons required for preservation of the multivoltine silkworm can be reduced to half per year.

• Journal of Sericultural and Entomological Science
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• v.23 no.1
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• pp.38-46
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• 1981

In order to investigate a suitable carbohydrate-resources and the activities of starch decomposing enzymes in artifical diet of silkworm, the experiment was undertaken by adding eight kinds of starch in the diet of silkworm. Major characters and zymograms of amylase in body organs were studied by electrophoresis. The results obtained are as follows: 1. Starches of rice, barey and millet were comparatively good for maintaining practical characters of silkworm. 2. It is assumed that no treatments are need to increas purity of starch resources for artificial diet of silkworm. It was found that starch amounts adding to artificial diet are moeerate ranging 12 to 18 percent as dry weight. 3. Regardless of kinds of starch and varieties of silkwormr sametype of electrophoresis zymogram for amylase was resulted as three bands in hemolymph, four bands in intestine and two bands in intestine and two bands in silkgland. There was no band in the digestive juice. In case of 18 percent addition of starch and check plots, no amylose change was investigated in the hemolymph.