• Proceedings of the Korean Society of Crop Science Conference
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• 2019.04a
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• pp.147-147
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• 2019

• Journal of agriculture & life science
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• v.44 no.1
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• pp.9-15
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• 2010

Barley, a monocotyledonous plant, is relatively recalcitrant to the process of Agrobacterium-mediated genetic transformation. In this study, seedlings of six barley cultivars (Keunal-1-Ho, Saessal, Ol, Saechalssal, Seodunchal and Pungsanchalssal) were injured using alkali, oxidizing or reducing agents. They were then transformed using Agrobacterium via vacuum infiltration for the analysis of comparative GUS gene expression. It was determined that chemical injuries causing a slight growth retardation could overall enhance the GUS transformation rate. Hydrogen peroxide was determined to be the most effective.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.04a
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• pp.99-99
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• 2022

• Korean journal of applied entomology
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• v.61 no.1
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• pp.249-254
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• 2022

Silkworms, which have for long been used as an insect resource for industrialization, have recently attracted attention as potential bio-factories for the production of novel biomaterials. In this regard, material production is typically achieved based on transformation technology, mediated via microinjection, in which a target gene is inserted into eggs containing an embryo. However, an essential step in the microinjection procedure is egg fixation, which can be a time-consuming and laborious task. Therefore, in this study, using the 3DCADian program, we adopted a 3D printing approach to model egg liners and glue drawers, which can contribute to facilitating egg alignment and fixation, thereby enhancing transformation efficiency by reducing time consumption and fatigue. After rendering using Fusion 360, the two supplementary tools were produced by printing with nylon resin (PA12) and Sinterit Lisa Pro. Subsequent analysis of the time required to fix eggs on glass slides using the two manufactured tools, revealed that the processing time was reduced by approximately 18.6% when the two tools were used compared with when these tools were not used. These innovations not only reduced fatigue but also contributed to more effective use of the microscope and manipulator for microinjection. Consequently, we believe that with additional research and refinement, the egg liner and glue drawer developed in this study could be used to enhance silkworm transformation efficiency and study similar transformation systems in other industrial insects.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.04a
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• pp.53-53
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• 2022

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.275-282
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• 2010

Chloroplast transformation in higher plants offers many attractive advantages over nuclear transformation, including a high-level accumulation of foreign proteins, multi-gene expression in single transformation event via transgene stacking in operons and no position effect due to site-specific integration of transgenes by homologous recombination. Most importantly, chloroplast transgenic plants are eco-friendly because their transgenes are maternally inheritance in most crop plants. However, chloroplast transformation system has limited success in crops alike nuclear transformation. In the past two decades, great progress has been made to overcome the limitations of chloroplast transformation, thus expending chloroplast bioreactor to several important crops including soybean, carrot, lettuce, and oilseed. Therefore, it has become possible that chloroplast transformation of crops can be used not only for the improvement of agronomic traits, but also for the production of vaccines and high valuable therapeutic proteins in pharmaceutical industry.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 2001.05a
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• pp.72-78
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• 2001

농업 유전공학 기술은 생산성 향상, 환경보전, 식품의 안정성 및 품질향상에 기여함은 물론 농업의 경쟁력을 높일 수 있는 유일한 대안으로 인식되고 있다. 전 세계적으로 유전자 재조합 작물의 경작지는 2000년 한해동안 지난해 같은 기간에 비해 11% 증가하였으며 이는 1996년 대비 25배 증가하였고, 선진국과 개발도상국은 각각 2%, 51% 1999년 대비 증가하였다. 1983년 유전자 재조합에 의한 식물의 형질전환이 성공한 뒤 종묘업계는 형질전환 종자개발과 보급에 열중하고 있으며 종자시장에 형질전화 품종이 차지하는 비율은 2000년 30억 달러에서 2010년이면 전체의 60%인 200억 달러에 이를 것으로 전망된다. 1995년 제초제 저항성 콩(라운드업레디콩)이 농가에 보급된 이후 2000년 형질전환품종 재배면적이 3990만 ha에 이르렀고 1997년 미국과 캐나다는 옥수수, 대두, 면화, 감자, 유채 등의 형질전환 품종 재배로 각각 3억1400만 달러, 5300만 달러를 벌어들였음. 형질전환 품종의 보급 증가속도는 소비자들의 GMO에 대한 거부반응으로 다소 주춤한 상태이다. 그러나 최근 종자회사들은 생태계 위해성 논란을 피해갈 수 있는 연구로 이러한 상황을 돌파하려 하고 있다. 우리나라에서도 유전자변형 생물체에 관한 법률이 제정되었으며 많은 대학과 연구소에서 형질전환 연구가 꾸준히 이루어지고 있고 최근 제초제 저항성 벼와 바이러스 저항성 감자가 개발돼 GMO 안정성 점검에 들어가 있고, 살충성 배추, 혈압강하 토마토, 지방산 강화 들깨, 병저항성 고추 등도 실험실과 포장에서 재배되고 있다. 이르면 4-5년 뒤 형질전환 작물들이 농가에 보급될 전망이다. 이처럼 체크 툴은 Firewall의 수비능력을 보강하는 위치에 있다고 생각할 수 있다.다. 4 장에서는 3장에서 제기한 각각의 문제점에 대해 RAD 의 관점에 비추어 e-business 시스템의 단기개발을 실현하기 위한 고려사항이나 조건 해결책을 제안한다. 본 논문이 지금부터 e-business 를 시작하려고 하는 분, e-business 시스템의 개발을 시작하려고 하는 분께 단기간의 e-business 실현을 위한 하나의 지침이 된다면 다행이겠다.formable template is used to optimize the matching. Then, clustering the similar shapes by the distance between each centroid, papaya can be completely detected from the background.uage ("Association of research for algorithm of calculating machine (1992)"). As a result, conventional NN and CNN were available for interpolation of sampling data. Moreover, when nonlinear intensity is not so large under the field condition of small slope, interpolation performance of CNN was a little not so better than NN. However, when nonlinear intensity is large under the field condition of

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.300-301
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• 2000

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.115-115
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• 2018

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.96-96
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• 2019