• The Korean Journal of Pesticide Science
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• v.11 no.4
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• pp.261-267
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• 2007

The acute toxicity of several pesticides on 4 Korean water flea was investigated to develop a new standard species used for ecological risk assessment of pesticide. Four Korean freshwater cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa and Simocepholus vetulus were exposed to five different pesticides during 48 hours to compare their sensitivity with a standard test species, Daphnia magna, endorsed formally by the major international organizations. The synthetic pyrethroid, fenpropathrin was the most toxic pesticide to cladocerans. Diazinon, carbofuran, iprodione and myclobutanil were in the order of their toxicity to cladocerans tested. There was no consistent difference in sensitivity to five pesticides for four Korean cladocerans tested. In conclusion, the ecological risk assessment using single species toxicity referred to base set data should not be enough to protect to every species in the field environment.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.11 no.3 s.96
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• pp.25-28
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• 1990

농약에 관여하는 사람은 모두 홍보요원이 되어, 농민에게는 농약을 어떻게 사용해야 농약중독을 막고 안전한 농산물을 생산하며 쾌적한 자연환경을 보존할 수 있는지, 도시민에게는 농약의 독성이 어느 정도이고 농산물 및 식품잔류농약이 인체에 어느 정도 위해성이 있는지 알려줘야 할 것이다.

• The Korean Journal of Pesticide Science
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• v.5 no.4
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• pp.57-61
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• 2001

Tank mixing application of pesticides has been used to reduce labor and to control wide spectrum of pests, but it may cause significant pesticide poisoning on human and animals due to carelessness. The toxic response of pesticides for the tank mixtures and mixtures was investigated to determine acute toxicity and enzymatic change using experimental animals. Acute oral toxicity and acute dermal toxicity were tested by RDA test guideline. The $LD_{50}$ was calculated by probit analysis method and cholinesterase was measured with automatic analyzer. The toxicities were generally higher than estimated toxicities in tank mixing and mixture. Serum cholinesterase activity was inhibited more than expected at the dose levels of 1/5, 1/10 and 1/20 of $LD_{50}$. Therefore, the results of this study showed that acute toxicity caused by the pesticide mixtures should be considered before the tank mixing method is applied.

• Agrochemical news magazine
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• v.22 no.7 s.170
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• pp.16-19
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• 2001

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.12 no.3 s.102
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• pp.27-34
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• 1991

• Agrochemical news magazine
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• v.25 no.1 s.194
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• pp.8-11
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• 2004

• The Bimonthly Magazine for Agrochemicals and Plant Protection
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• v.13 no.2 s.107
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• pp.92-93
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• 1992

• The Korean Journal of Pesticide Science
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• v.18 no.1
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• pp.8-13
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• 2014

This study evaluated in vitro cytotoxicity of 5 pesticides, including 2 herbicides, 2 germicides, and an insecticide, as an alternative to the fish acute toxicity test. The in vitro cytotoxicity was tested using a neutral red uptake (NRU) assay with epithelioma papulosum cyprini (EPC) cells that originated from the epidermal tissue of Cyprinus carpio (common carp). An in vivo fish acute toxicity test was conducted according to OECD Test Guideline No. 203 using Aphyocypris chinensis (Chinese bleak), Oryzias latipes (Japanese medaka), and C. carpio. The results showed that the sensitivity of the cell viability assay for the pesticides was similar to the fish acute test in ranking order despite having approximately 10 times less absolute sensitivity. The $r^2$ correlation values were calculated as 0.38 (p = 0.26), 0.76 (p = 0.05) and 0.90 (p = 0.01) for A. chinensis, O. latipes, and C. carpio, respectively. These results suggested that the potential of EPC cell viability assay as an alternative to the fish acute toxicity test due to their good correlation and NRU assay is expected to serve as a useful tool for predicting acute fish lethality for pesticides if further studies with a large set of pesticides are conducted.

• The Korean Journal of Pesticide Science
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• v.14 no.3
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• pp.226-234
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• 2010

Foliage residue toxicity experiment was performed against honeybee (Apis mellifera) with bifenthrin, a synthetic pyrethroid insecticide with strong acute contact toxicity and imidacloprid, a neo-nicotinoid insecticide with strong acute oral toxicity to know the honeybee toxicity at the residue level on the leaves of alfalfa and apple. Also, the formulation differences to honeybee toxicity were investigated with WP (2%) and EC (1%) of bifenthrin and WP (10%) and SL (4%) of imidacloprid. Generally, foliage residual toxicity of honeybee and residual amounts of tested insecticides was higher in alfalfa leaves with large leaf area per unit weight than in apple leaves. While on the other hand, the only bifenthrin WP treatment showed higher honeybee toxicity on apple leaves than alfalfa. Although imidacloprid showed higher residue amounts ranged $4.9{\sim}25.4\;mg{\cdot}kg^{-1}$ than bifenthrin ranged $0.6{\sim}12.7\;mg{\cdot}kg^{-1}$ on the leaves, the residual toxicity to honeybee was lower than bifenthrin because of its strong penetration character. In conclusion, the residual toxicity of insecticide to honeybee could be affected by the contact and vaporized toxicity of chemical, the residual amounts on the surface of leaves, and the leaf area per unit weight and formulation differences.

• The Korean Journal of Pesticide Science
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• v.16 no.1
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• pp.78-83
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• 2012

The recommended species in fish acute toxicity test for pesticide registration in korea are common carp (Cyprinus carpio), killifish(Oryzias latipes) and loach(Misgurnus anguillicaudatus). But most of fish toxicity was evaluated using common carp. The common carp has several problems such as difficulty of room breeding, difference of sensitivity to breeding condition, limited experimental period, etc. For that reason, it is necessary to reexamine of test species. The present study investigated the sensitivity and correlation of $LC_{50}$ values that determined using the acute toxicity test with common carp and killifish. And this study is a realistic approach to evaluated appropriate species for fish acute toxicity test of pesticide. As a result, correlation coefficient(R) of the commom carp and killifish was high correlation of 0.8480 and the $LC_{50}$ values was not significant in the interspecies sensitivity. When the $LC_{50}$ values of common carp and killifish was compared with fish toxicity guideline in Korea, fish toxicity class has fluctuated in 18 pesticides with not very special tendency. Therefore, it is necessary to review of killifish has more advantage to standardization of fish culture and in the side of correlation and sensitivity of acute toxicity.