• Journal of Life Science
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• v.27 no.7
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• pp.746-753
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• 2017

In order to rapidly identify four drums species, Larimichthys polyactis, L. crocea, Atrobucca nibe, and Pseudotolithus elongates, a highly efficient and quick method has been developed using multiplex polymerase chain reaction (PCR) with species-specific primers. Around 1.4 kbp of the mitochondrial COI gene sequences from the four drums species were aligned, and species-specific forward primers were designed, based on the single nucleotide polymorphism (SNP). The optimal conditions for PCR amplification were selected through cross-reactivity, using a gradient PCR method. The PCR results demonstrated species-specific amplification for each species at annealing temperatures between 50 and $62^{\circ}C$. Multiplex species-specific PCR (MSS-PCR) amplification reactions with four pairs of primers were performed for sixteen specimens of each species. MSS-PCR lead to a species-specific amplification of a 1,540 bp fragment in L. polyactis, 1,013 bp in A. nibe, 474 bp in L. crocea, and 182 bp in P. elongates, respectively. The four different sizes of each PCR product can be quickly and easily detected by single gel electrophoresis. The sensitivity of the MSS-PCR of the DNA was up to $0.1ng/{\mu}l$ as a starting concentration for the four different species tested. These results suggest that MSS-PCR, with species-specific primers based on SNP, can be a powerful tool in the rapid identification of the four drums species, L. polyactis, L. crocea, A. nibe, and P. elongates.

• Korean Journal of Environment and Ecology
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• v.23 no.1
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• pp.66-77
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• 2009

This study aims at classifying and interpreting on the forest vegetation located in the whole area of Mt. Boryeonsan in Noeunmyeon, Chungju city, Chungcheongbuk Province, Korea using the methodology of the ZM school of phytosociology. The forest vegetation was classified into one Larix leptolepis afforestation, five secondary communities including Qurecus acutissima community, Pinus densiflora community, Quercus aliena community, Quercus serrata community, Quercus variabilis community and one association, Ainsliaeo-Quercetum mongolicae Song et al. 1999(Rhododendro schlippenbachii-Quercion mongolicae, Aceri pseudosieboldiani-Quercetalia mongolicae, Quercetea mongolicae). The research expatiated on the relationship between species composition of the forest communities and the environmental conditions, such as human interference, altitude, slope, topography and chemical properties of soil, and also analyzed the life form by each community. As a result, the Ainsliaeo-Quercetum mongolicae was judged as the most stable community among the forest communities identified here. On the other hand, a division of cluster analysis based on the dominance of the component species, appeared similar to the community units classified phytosociologically. Thus, it is inferred that the survey area will be dominated by the potential natural vegetation, Ainsliaeo-Quercetum mongolicae finally, if progressive succession is advanced continuously.

• Journal of Life Science
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• v.26 no.9
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• pp.1007-1014
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• 2016

A highly efficient, rapid, and reliable multiplex polymerase chain reaction based method for distinguishing ten species of genus Cynoglossus (C. senegalensis, C. abbreviates, C. macrolepidotus, C. arel, C. semilaevis, C. interruptus, C. joyneri, C. lingua, C. robustus, and C. monodi) is described. The species-specific primer sets were designed base on the cytochrome oxidase subunit I gene (1,500 bp). The optimal PCR conditions and primers were selected for ten of Cynoglossus species to determine target base sequences using single PCR. Multiplex PCR using the ten pairs of primers either specifically amplified a DNA fragment of a unique size or failed, depending on each species DNA. The length of amplification fragment of 208 bp for C. senegalensis, 322 bp for C. abbreviates, 493 bp for C. macrolepidotus, 754 bp for C. arel, 874 bp for C. semilaevis, 952 bp for C. interruptus, 1,084 bp for C. joyneri, 1,198 bp for C. lingua, 1,307 bp for C. robustus, and 1,483 bp for C. monodi with the species-specific primers, visualized by agarose gel electrophoresis, allowed perfectly distinction of the Cynoglossus species. The multiplex PCR assay can be easily performed on multiple samples and attain final results in less than 6 hours. This technique should be a useful addition to the molecular typing tools for the tentative identification of Cynoglossus species.

• Journal of Digital Contents Society
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• v.19 no.1
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• pp.93-102
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• 2018

This study aims to present policy implications by analyzing information security behavior factors of internet users. The research model, based on PMT and UTAUT2, consists of perceived threat, severity, social influence, self-efficacy, experience and habits, PC and privacy behaviors, security behaviors on new services and set demographic characteristics, use places of internet, use of paid products, and experiences of accident as moderate variables to analyze the effect on security behavior. The results showed that perceived severity, self-efficacy significantly influenced on experience and habits, and experience and habits and self-efficacy had a high influence on PC and privacy behavior. Also, PC and privacy behaviors have a high impact on security behavior of new services. Age, income, use of paid products, and experience of accidents have a moderating effects on security behaviors. The results of this study are expected to help policy decision making to improve the level of information security of internet users.

• Journal of the Korean School Mathematics Society
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• v.17 no.2
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• pp.139-166
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• 2014

The purpose of the study is to enhance the teaching competence for pre-service elementary teacher by using DGE in order to enhance SMK and PCK for them. To do this, we investigated the initial SMK and PCK for 23 pre-service elementary teachers, the reality of implementation activity of DGE and the change of SMK and PCK after quest activity by DGE. As results, 3 pre-service elementary teachers made errors which are misunderstanding a general angle as special angle, an excessive jump of logic and a circulation logic in the aspect of an initial SMK. In the aspect of contents of PCK, most of pre-service elementary teachers proposed teaching focused on the character using in the problem solving. And most of pre-service elementary teachers proposed teaching methods which are based on explanation, measurement and material manipulation. The reality of implementation activity of DGE was classified 4 cases which are a difficulty in understanding the concept of dynamics and embodying in DGE, an obsession about construction of $75^{\circ}$ and generalization, a difficulty in interpreting 'folding activity' mathematically and a good implementation activity. After quest activity by DGE, the case which is misunderstanding a general angle as special angle could be improved, but the others are not. And after quest activity by DGE, most of pre-service elementary teachers still proposed teaching focused on the character using in the problem solving in the aspect of contents of PCK, and some of pre-service elementary teachers added only teaching methods which are involving visual confirmation by GSP. From these results, we could extract some pedagogical implications helping pre-service teachers to reinforce SMK and PCK by DGE.

• KEPCO Journal on Electric Power and Energy
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• v.6 no.3
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• pp.285-288
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• 2020

The demand for coating technology on electric power equipment that has arisen from such issues regarding the attaching of illegal advertisements and posters to electric power distribution boxes such as TR, SW, etc. in down town areas seeks to produce functional coating surfaces using polymers and nano-materials that will result in improvements in self-cleaning performance and greater stability even under harsh environmental conditions. KEPCO-coatings consist of copolymerized acrylic resin and methacryl-modified reactive silicone that are able to chemically combine, which results in performance improvement without any leakage of of silicone, thus contributing to its properties of high-stability. Thus, the research team has also started long-term on-site testing on 9 electric power distribution spots around a city center in cooperation with the KEPCO Daeduck-yusung branch. The KEPCO-coating technology could advance the best coating materials and processes to meet appropriate circumstances for a variety of outdoor damage environment. It is also predicted that KEPCO could be possible to expand international electric maintenance markets and to arrange business platforms if KEPCO would achieve its original technology (IPs) by the means of upgrading in self-cleaning coating technology and obtaining long-term on-site test records from nationwide electric facilities.

• Analytical Science and Technology
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• v.28 no.4
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• pp.278-287
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• 2015

Phosphodiesterase type 5 inhibitors (PDE-5 inhibitors) are used in the treatment of erectile dysfunction. In recent years, a number of reports have been conducted on dietary supplements contaminated with PDE-5 analogues. In this study, 58 analogues of PDE-5 inhibitors were sorted into five groups: tadalafil, sildenafil, hongdenafil, vardenafil, and other analogues. These analogues were then evaluated using a liquid chromatography-quadrupole-time of flight mass spectrometry (LC-QTOF-MS) electrospray ionization mass method. Each compound has a unique fragmentation ion, which can be easily analyzed qualitatively. The fragmentation pathways of the analogues were elucidated based on the QTOF-MS and MS/MS data. Common ions were confirmed for each group by analyzing the structural characteristics and fragmentation pathways. Specifically, common ions were observed at m/z 169.08 and 135.04 (tadalafil analogues), m/z 311.15 and 283.12 (sildenafil analogues and hongdenafil analogues), and m/z 312.16 and 151.09 (vardenafil analogues). The advantage of this method is that the structure of unknown components can be determined by interpreting the product ions. Hence, the developed method can be used for the identification of unknown compounds. Fragmentation pathways may also aid in the detection and identification of PDE-5 inhibitor analogues.

• Restorative Dentistry and Endodontics
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• v.34 no.3
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• pp.199-207
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• 2009

The objective of this study was to evaluate the effect of color measuring instrument by measuring the color of dental composite resins. Nine shade light cured composite resin disks were prepared (diameter : 15 mm, thickness : 4 mm). CIE $L^*a^*b^*$ color scale of each disk was measured with 3 different types of spectrophotometer [MiniScan XE plus (Model 4000S, Hunter Lab, USA), CM-3500d (Minolta, Japan) and Specbos 2100 Miniature VIS Reflection spectrometer (Serial No: 319416, JETI Technishe VIS Instrumentic GmbH. Germany)]. Miniscan XE Plus and CM-3500d using identical measuring geometry with different size of viewing aperture. But Specbos 2100 using different measuring geometry. Within the limitation of this study, there were color difference (${\Delta}E^*$) from 2.4 to 7.8 between Miniscan XE Plus and CM-3500d, but $L^*$, $a^*$, $b^*$ values showed the high correlation. However, there were great color difference (${\Delta}E^*$) in the extent of about 20 between instruments with the different measuring geometry. Therefore, color scale measured by color measuring instrument should be used as a relative value rather than an absolute value in the field of dentistry.

• KIPS Transactions on Software and Data Engineering
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• v.5 no.11
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• pp.527-534
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• 2016

The emergent information society requires the talent for creative thinking based on problem-solving skills and comprehensive thinking rather than simple memorization. Therefore, the Korean curriculum has also changed into the direction of the creative thinking through increasing short-answer questions that can determine the overall thinking of the students. However, their scoring results are a little bit inconsistency because scoring short-answer questions depends on the subjective scoring of human raters. In order to alleviate this point, an automated scoring system using a machine learning has been used as a scoring tool in overseas. Linguistically, Korean and English is totally different in the structure of the sentences. Thus, the automated scoring system used in English cannot be applied to Korean. In this paper, we introduce an automated scoring system for Korean short-answer questions using predictability and unanimity. We also verify the practicality of the automatic scoring system through the correlation coefficient between the results of the automated scoring system and those of human raters. In the experiment of this paper, the proposed system is evaluated for constructed-response items of Korean language, social studies, and science in the National Assessment of Educational Achievement. The analysis was used Pearson correlation coefficients and Kappa coefficient. Results of the experiment had showed a strong positive correlation with all the correlation coefficients at 0.7 or higher. Thus, the scoring results of the proposed scoring system are similar to those of human raters. Therefore, the automated scoring system should be found to be useful as a scoring tool.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.149-154
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• 2015

A new Phalaenopsis cultivar 'SM 333' was bred by Sangmiwon Orchid, Korea, which produces young plants through tissue culture techniques. The new cultivar 'SM 333', showing the phenotype of multiflora with pink color and small, multibranching-type characteristics, was derived from crossing between Phalaenopsis 'Odoriko' and 'Be Tris'. An elite individual number '02-03-33' later termed 'SM 333' was selected among about 300 individual progenies, based on an intensive selection process covering vegetative and flowering distinctiveness over more than 2 years. In year 2004-2005, the 1st and 2nd characteristic analyses were carried out through performance and uniformity tests. 'SM 333' shows flower color that is bright clean pink (RHS # RP69D) and flower shape that is formal type with 5.0 and 5.8 cm in flower height and width, respectively. 'SM 333' is regarded as raceme flower type suitable for the small casual flower market. The leaves of 'SM 333' grow horizontally and about 20.8 cm in length and 6.5 cm in width. This cultivar also possesses no genetic variation, and is amenable to fast in vitro propagation and easy growth due to its vigorous growth habit. This 'SM333' was registered (Reg. # 2916) with Korea Seed & Variety Service (KSVS) on 1st December, 2009, and the plant breeder's right is currently controlled by Sangmiwon Orchid Company, Korea.