• Journal of Aquaculture
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• v.19 no.1
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• pp.47-51
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• 2006

Experiments were performed to find out the physico-chemical properties of milt and sperm motility in various storage conditions using starry flounder (Platichthys stellatus). The average sperm concentration and spermatocrit in stripped milt were $6.00{\pm}0.98{\times}10^8/mL\;and\;72{\pm}5$, respectively. The osmolality and pH were $337{\pm}9mmol/kg$ and $7.7{\pm}0.1$, respectively. The sperm of starry flounder was preserved with artificial seminal plasma (ASP), Stein's solution (SS) and marine fish Ringer's solution (MFRS) at $0^{\circ}C,\;2^{\circ}C,\;and\;4^{\circ}C$. The most effective condition for cold storage was SS at $0^{\circ}C$, and the preserved sperm remained motile for 30 days.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.20 no.3
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• pp.97-102
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• 2014

It was mathematically analyzed at which steps to activate TTI in the cold chain for Alaska pollack, assuming that the performance of a commercial TTI product, and Fresh-check, could not always be optimized for the pollack. Three places were selected for the TTI activation, such as on fishing ship, Busan cooperative fish market, and mart. First, the kinetic and Arrhenius temperature dependent models were experimentally built under isothermal conditions. The color index of TTI and the level of Pseudomonas spp. of pollack were measured at time intervals. Second, the resultant models were used in the mathematical calculations for dynamic temperature conditions included in the cold chain. As a result, the TTI activated at the mart place showed the best agreement between the spoilage time of the pollack and the time for the TTI color to reach its end-point. It was therefore found that it is practically important to optimally select the TTI activation place or time when using a commercial TTI product.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.151-155
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• 2004

This study was performed to develop the method of differentiation fresh and frozen meat by using the measurement of mitochondrial malate dehydrogenase. The principle of this experiment is based on the fact the enzyme proteins associated with mitochondria membrane could be released by freezing. The methods were studied by measurements of protein concentration of meat press juice, WHC (water-holding capacity), drip loss and mitochondrial malate dehydrogenase enzyme activity. Samples were stored at 4$^{\circ}C$ and -18$^{\circ}C$ during storage period, respectively. Protein concentration of meat press juice was ranged from 8.5 mg/mL to 12.7 mg/mL and increased by freezing below at -18$^{\circ}C$(p<0.05). The WHC was not significantly different between fresh meat and frozen chicken meat (p＞0.05). The amount of drip loss of fresh and frozen chicken meat at 4$^{\circ}C$ and -18$^{\circ}C$ was not significantly different (p＞0.05). Mitochondrial malate dehydrogenase activity of frozen meat (-18$^{\circ}C$) was significantly higher (p＜0.05) than that of fresh meat. Also, enzyme activity of frozen meat was maintained at the same level after 3 minutes reaction. But fresh meat had not this reaction. From these results, it suggests that mitochondrial malate dehydrogenase can be used as a promising enzyme to differentiate between fresh and frozen meat.

• Journal of Food Hygiene and Safety
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• v.35 no.2
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• pp.170-176
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• 2020

We investigated the pH, volatile basic nitrogen (VBN), microbial changes and dominant microbes in raw beef, wet-aging beef, and dry-aging beef after the meat had been stored in a refrigerator. The count of mesophilic bacteria was 3.3-3.9 log CFU/g in raw beef and dry-aging beef, and 5.4 log CFU/g in wet-aging meat. After 18 days of refrigeration, the mesophilic bacterial count in raw and aging beef increased to 6.1-6.4 log CFU/g. In wet-aging beef, the number of lactic acid bacteria increased from 4.5 log CFU/g to 6.0 log CFU/g at refrigeration temperature. However, lactic acid bacteria were not detected in dry aging beef. Major foodborne pathogens such as Salmonella spp, Listeria monocytogenes, and Escherichia coli (EHEC) were not detected. Based on the legal standard for mesophilic bacteria count, the estimated shelf-life of aged beef was less than 12 days and the average VBN was 15 mg%. The dominant microorganisms varied between the different types of meat. In raw meat, Staphylococcus saprophyticus was the dominant microorganism, and as the VBN increased, Carnobacterium divergens dominated. In wet-aging beef, Carnobacterium divergens dominated during the initial days of refrigeration after which the number of Lactobacillus sakei increased. Dermacoccus nishinomiyaensis was initially the dominant microbe in dry-aging beef, after which Pseudomonas fragi dominated. In addition to the role of specific bacteria in the early stage of decay, it is thought that microorganisms can be utilized for safe distribution and storage of matured meats by conducting research on changes in rot, fragrance analysis, and changes of ingredients in matured meats.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.9
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• pp.465-473
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• 2018

A cabinet-door integrated finite element model for a built-in side-by-side refrigerator with an ice dispenser and home bar was constructed, and its deformation was analyzed by ANSYS. As loads, the food load in the shelf and baskets, and thermal load occurring during the normal operation condition were considered. From results of the analyses, the door height difference (DHD) and door flatness difference (DFD) between the two doors, and the increase in the gap of the door gasket, which affects the sealing of cool air in the cabinet, were derived. As results of an evaluation of the differences, the DHD and DFD under the assembled condition satisfied the acceptance criteria of the manufacturer. The food and thermal loads increased the DHD and DFD due to thermal deformation, and the DFD increased significantly. In addition, the increase in the gap of door gasket located between the cabinet and doors was derived from the results of displacements under the food and thermal loads. The evaluation showed that the maximum increase in gap appeared at the left edge of the freezing compartment gasket, which satisfied the acceptance criteria of the manufacturer.

• Journal of the KSME
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• v.25 no.2
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• pp.106-110
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• 1985

압축기의 체적효율, 압축효율에 관련된 부분에 대해서만 검토를 하였으며, 이는 주로 실린더 내 외의 냉해의 압력, 온도 등의 변화과정과 관련이 있다. 압축기의 모델로는 그림 1과 같은 스카 치-요크(Scotch-Yoke)형의 소형 완전 밀폐형 왕복식 압축기를 사용하였다. 그리고 냉매는 R-1 2를 사용하였다.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2000.11a
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• pp.117-117
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• 2000

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 1996.11a
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• pp.94-94
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• 1996

• Food Industry
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• s.219
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• pp.24-60
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• 2011

${\bigcirc}$ 본 업소는 수산물가공품(냉동어류, 냉동연체류, 조미가공품)을 생산하는 식품제조 가공업소로서 총 5명의 인원이 수산물가공품 8개 제품을 생산하여 매출액은(약 00원)이며 주로 집단급식소에 판매하고 있다. ${\bigcirc}$ 본 업소의 수산물가공품은 냉동생선 해동(또는 냉장생선 이용), 전처리(냉장제거 등) 이후 세척 후 급냉하여 포장하거나(냉동어류 및 냉동연체류) 가열 후 포장하여 급냉하는(조미가공품) 공정을 거쳐 생산된 제품으로 원료 취급과정에서의 오염이나 불충분한 가열, 교차오염 등으로 식중독균(병원성대장균, 황색포도상구균 등)과 원료 및 제조과정에서 이물(금속 등)이 혼입할수 있으며, ${\bigcirc}$ 이로 안한 주요클레임 발생사례는 최근 3년간 관계당국으로부터 대장균군 검출 3건, 소비자클레임 2건이 있었다. - 연도별 주요 클레임내용은 '08년도에는 대장균군 검출 3건, '09년도 냉동어류에서 날벌레 이물 검출 1건, '10년도에는 냉동연체류에서 철수세미 1건 이었다. ${\bigcirc}$ 이러한 위해발생을 사전에 예방하기 위해 중점관리해야 하는 공정은 세척공정(냉동어류 및 냉동연제류) 및 가열공정(조미가공품)으로 판단되며, 금속 등의 이물 혼입 또한 중점적으로 관리할 필요성이 있다. ${\bigcirc}$ 본 업소에서 생산하는 냉동어류 및 냉동연체류는 세척공정에서 세척시간과 세척수량, 원물량을 각각 설정하여 CCP 1로 관리하고 2시간마다 모니터링하고 있으며, ${\bigcirc}$ 조미가공품은 가열온도와 시간을 각각 설정하여 CCP 2로 관리하고 있으며, 품온이 $85^{\circ}C$이상 유지할수 있도록 2시간마다 모니터링하고 한계기준 이별여부를 기록하도록 하고 있다. ${\bigcirc}$ 또한 금속검출공정을 CCP 2로 관리하여 2mm${\varphi}$ 이상의 금속이물 혼입여부를 상시 확인하고 있으며, 금속검출기가 정상 작동여부를 4시간마다 모니터링하고 기록하도록 하고 있다. ${\bigcirc}$ 종합적인 공정 및 일반위생관리를 위해 개인위생 상태 냉동 냉장고 온도 확인 등 총 29개 항목에 대하여 정기적 점검(매일 17 주간 4 월간 4 분기 1, 연간 3)을 실시하고 있으며, ${\bigcirc}$ 따라서 지속적인 모니터링을 통해 미흡사항의 원인으로 파악하고 문제점 제거를 위해 체계적이고 지속적인 관리가 필요하다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.7
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• pp.994-1001
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• 2012

Quality changes of pre-processed garlic, peeled and chopped, were analyzed during storage at $-18^{\circ}C$ or $2^{\circ}C$ for 30 days and at $20^{\circ}C$ for 3 days only for chopped garlic. As storage time increased, Hunter L values decreased and a, b values increased, indicating browning regardless of the pre-process type and storage temperature. Decay and sprouting rates of peeled garlic during storage at $2^{\circ}C$ significantly increased while those of peeled garlic were maintained during storage at $-18^{\circ}C$. Weight loss of peeled garlic during storage was greater at $-18^{\circ}C$ than at $2^{\circ}C$. Hardness of peeled garlic rapidly decreased by half from 1.04 kg to 0.58 kg by freezing, and it did not significantly change during the storage period. Viable numbers of total aerobic bacteria of peeled and chopped garlic did not significantly change during the storage period at $2^{\circ}C$ but were reduced at $-18^{\circ}C$. Total aerobic bacterial count of chopped garlic stored at $20^{\circ}C$ slightly increased during the storage period. Pyruvic acid content of chopped garlic was almost 2.5 times higher than that of peeled garlic at the initial stage (463.87 ${\mu}mol/g$ and 190.52 ${\mu}mol/g$, respectively). As storage time increased, pyruvic acid content of peeled garlic increased while that of chopped garlic decreased. These results indicate that pre-process type and storage temperature affected the quality changes of garlic during storage.