• Title/Summary/Keyword: 냉각단계

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Effect of Incubation Time after Cooling on the Meiotic Spindle and Chromosomes of Mouse Oocytes (냉각 후 배양시간이 생쥐 난자의 방추체와 염색체에 미치는 영향)

  • Yu I.
    • Journal of Embryo Transfer
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    • v.19 no.3
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    • pp.283-289
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    • 2004
  • This study was conducted to determine the effects of incubation time after cooling on mouse meiotic spindle and chromosome alignment and the optimal incubation time for their restoration. Oocytes at the metaphase II were obtained from superovulated mice. Control oocytes were held at 37$^{\circ}C$ during the experiment. Oocytes were rapidly cooled to $0^{\circ}C$, held for 30 minutes, warmed and incubated at 37$^{\circ}C$ for 5, 15, 30, 60 and 120 minutes, respectively. The morphological features of spindle and chromosomes in oocytes were evaluated by immunofluorescent staining. Meiotic spindle of control oocytes exhibited a normal-looking bipolar configuration(barrel-shaped) and highly fluorescent microtubles. The chromosomes were clustered in a discrete bundles at metaphase plate. Disassembly of meiotic spindle and chromosome dispersion were occurred immediately after chilling of oocyte. Fluorescence intensity index(FIS), normal chromosomes aligned and normal spindle configuration were compared according to incubation time at 37$^{\circ}C$. Restoration of a barrel-shaped spindle and normal chromosome alignment was occurring after 5 minutes incubation at 37$^{\circ}C$, improved as a incubation time increased, and decreased gradually after 120 minutes incubation(P<0.05). The optimal incubation time for restoration of meiotic spindle and chromosomes in cooled oocytes was 60 minutes.

The effect of cooling rate on the nuclei of OISF formation in Si single crystals (실리콘 단결정에서 산화적층결함의 핵생성에 미치는 냉각속도의 영향)

  • 하태석;김병국;김종관;윤종규
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.6 no.3
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    • pp.360-367
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    • 1996
  • The OISF (Oxidation Induced Stacking Fault)is expected to affect the electrical properties in Si single crystals, and the nuclei of OISF are believed to be formed during the crystal growing process. Initial oxygen concentration, dopant type and its density, and cooling rate are regareded as major factors on OISF formation. In this study, the variations of OISF density under various cooling rate were investigated. Si single crystal was heated to $1400^{\circ}C$ in Ar ambient and cooled down to room temperature at different cooling rate, using horizontal tube furnace. After that, they were oxidized at $1150^{\circ}C$, and then, OISF was observed with optical microscope. The relation between oxide procipitates and OISF nucleation was investigated by FTIR analysis. As a result, it was found that there exists the intermediate cooling rate range in which OISF nucleation is highly enhanced. And also, it was found that OISF nucleation is closely related with silicon oxide procipitation in Si single crystals.

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Effect of Injector Cooling on Ignition of Cryogenic Spray (분사기 냉각이 초저온 분무의 점화에 미치는 영향)

  • Kim, Do-Hun;Lee, Jin-Hyuk;Koo, Ja-Ye
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.40 no.3
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    • pp.222-229
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    • 2012
  • The cooling of a injector effects on the vapor pressure of cryogenic oxidizer spray, and it decides the phase transition point at the ignition process, when the combustion chamber pressure increases drastically. The phase transition of oxidizer spray affects the ignition characteristics, and several ignition tests with the LOx/$GCH_4$ uni-element coaxial swirl injector was performed in the different initial temperatures of oxidizer injector, in order to investigate the effect of injector cooling on the ignition transient characteristics. At the transition point of oxidizer phase, where the combustion chamber pressure increased over the LOx vapor pressure, the temporary quenching phenomenon of the flame occurred. The lower temperature of chilled down injector and tubing tends to move up the phase transition earlier.

Detection and Evaluation of Microdamages in Composite Materials Using a Thermo-Acoustic Emission Technique (열-음향방출기법을 이용한 복합재료의 미세손상 검출 및 평가)

  • 최낙삼;김영복;이덕보
    • Composites Research
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    • v.16 no.1
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    • pp.26-33
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    • 2003
  • Utilizing a thermo-acoustic emission (AE) technique, a study on detection and evaluation of microfractures in cross-ply laminate composites was performed. Fiber breakages and matrix fractures formed by a cryogenic cooling at $-191^{\circ}C$ were observed with ultrasonic C-scan, optical and scanning electron microscopy. Those microfractures were monitored in a non-destructive in-situ state as three different types of thermo-AE signals classified on the basis of Fast-Fourier Transform and Short-Time Fourier Transform. Thus, it was concluded that real-time estimation of microfracture processes being formed during cryogenic cooling could be accomplished by monitoring such different types of thermo-AEs in each time-stage and then by analyzing thermo-AE behaviors for the respective AE types on the basis of the AE signal analysis results obtained during thermal heating and cooling load cycles.

LOCA Analysis and Development of a Simple Computer Code for Refill-Phase Analysis (냉각재 상실사고 분석 및 재충진 단계해석용 전산코드 개발)

  • Ree, Hee-Do;Park, Goon-Cherl;Kim, Hyo-Jung;Kim, Jin-Soo
    • Nuclear Engineering and Technology
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    • v.18 no.3
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    • pp.200-208
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    • 1986
  • The loss of coolant accident based on a double-ended cold leg break is analyzed with the discharge coefficient (Ca) of 0.4. This analysis covers the whole transient period from the start of depressurization to the complete refilling of the core by using RELAP4/MOD6-EM and RELAP4/ MOD6-HOT CHANNEL for the system thermal-hydraulics and the fuel performance during the blowdown phase respectively, and RELAP4/MOD6-FLOOD and TOODEE2 during the reflood phase. A simple analytical method has been developed to account for the lower plenum filling by approximating steam-water countercurrent flows and superheated wall effects at the downcomer during the refill period. Based on the informations. at the time of EOB (end-of-bypass), the refill duration time and the initial reflooding temperature were estimated and compared with the results from the RELAP4/MOD6, resulting in a good agreement. In addition, some parametric studies on the EOB were performed. The form loss coefficient between upper head and upper downcomer was found to be sensitive to the occurrence of the spurious EOB. Appropriate form loss coefficients should be taken into account to avoid the flow oscillations at the downcomer. The analyses with the six and three volume core nodalizations, respectively, show much similar trends in the system thermal-hydraulic performance, but the former case is recommended to obtain good results.

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Comparison of Counter-Current Cooling and Pool Boiling System Through Modeling and Simulation of a Pilot-Scale Fixed bed Reactor for Dimethyl Ether(DME) Synthesis (Dimethyl Ether(DME) 합성을 위한 파일럿 규모의 고정층 반응기의 모델링과 모사를 통한 향류 냉각방식과 포화액체 풀비등 방식의 비교)

  • Song, Daesung;Go, Jae Wook;Yoon, En Sup
    • Korean Chemical Engineering Research
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    • v.47 no.4
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    • pp.446-452
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    • 2009
  • The behavior of a one-step fixed bed reactor which directly synthesizes dimethyl ether(DME) from Natural Gas was simulated. In the reactor, the prevention of the occurrence of hot spots which can cause deactivation of catalysts is pivotal, since methanol synthesis and dehydration reaction involved in the synthesis of DME are highly exothermic. Therefore, we simulated and compared performance of the reactor with counter-current cooling and pool boiling system that can be applied to a commercial plant. As a result, we found that counter-current cooling system is more effective in terms of CO conversion and DME productivity. However, pool boiling system can operate in a small temperature gradient that can decrease problems caused by hot spot. And, the system can operate in a safer range.

MIRIS 우주관측 카메라 비행모델 Passive Cooling Test

  • Park, Yeong-Sik;Mun, Bong-Gon;Cha, Sang-Mok;Lee, Deok-Haeng;Lee, Dae-Hui;Han, Won-Yong;Jeong, Ung-Seop;Lee, Chang-Hui;Park, Seong-Jun;Nam, Uk-Won;Ga, Neung-Hyeon;Park, Jang-Hyeon;Lee, Seung-U;Matsumoto, Toshio
    • The Bulletin of The Korean Astronomical Society
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    • v.35 no.2
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    • pp.56.1-56.1
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    • 2010
  • MIRIS(Multipurpose InfraRed Imaging System)는 과학기술위성 3호의 주 탑재체로서 2011년 발사예정인 다목적 적외선 카메라 시스템이다. MIRIS는 우주관측 카메라와 지구관측 카메라로 구성되어 있으며, 우주관측 카메라는 $0.9-2.0{\mu}m$ 영역에서 3.67 deg. x 3.67 deg. FOV로 우리 은하평면 survey 관측과 우주배경복사(CIB) 관측을 수행할 것이다. 현재 MIRIS는 비행모델 개발 마무리 단계에 있으며, 검교정 시험, 열-진공 시험, 진동 시험 등을 수행하고 나면 2010년 말 위성 본체와의 조립을 진행할 것이다. 우주관측 카메라는 궤도상에서 태양, 지구의 적외선 복사와 망원경과 검출기 주변에서 발생하는 열잡음을 줄이기 위해 냉각이 필요하며, 제한된 위성의 무게와 부피, 전력등의 요구조건들 때문에 망원경 및 구조체의 복사냉각(Passive Cooling) 방법을 선택하였다. Passive cooling으로 우주관측 카메라의 망원경이 200K 이하로 냉각되면, dewar에 설치된 소형 냉각기를 가동하여 적외선 센서를 80K로 냉각한다. 위성체 내벽과 우주관측카메라의 각 구조체들 사이의 복사를 차단하기위해 30층의 MLI를 적용 하였고, 각 구조체들간의 열전도를 최소화하기위해 GFRP supporter를 적용하였다. 이 실험은 천문(연)에서 자체 제작한 열-진공 챔버를 활용하여 진행하였으며, 이미 인증모델에 대한 passive cooling 실험을 두 차례 실시하였고, 그 실험 결과를 반영하여 최종 비행모델에 대한 실험을 수행하였으며, 그 실험 결과에 대해 논의 하고자 한다.

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Modified Simulated Annealing Algorithms for Optimal Seismic Design of Braced Frame Struvtures (2차원 가새골조의 최적내진설계를 위한 MSA 알고리즘)

  • Lee, Sang Kwan;Seong, Chang Won;Park, Hyo Seon
    • Journal of Korean Society of Steel Construction
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    • v.12 no.6
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    • pp.629-638
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    • 2000
  • With the positive features of simulated annealing algorithms such as simplicity of the algorithm and the possibility of finding global optimum solution, SA algorithm has been widely applied to structural optimization problems. However, the algorithms are far from practical applications in structural design or optimization of building structures due to requirement of a large number of iterations and dependency on cooling schedule and stopping criteria. In this paper, with the modification of annealing process and stopping criteria, a MSA algorithm is presented in the form of two phase annealing process for optimal seismic design of braced structures. The performance of the proposed algorithm has been illustrated in detail.

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크라이오 펌프 종합특성평가 시스템 기술개발

  • In, Sang-Ryeol;Jeong, Seung-Ho;Hwang, Pil-Seo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2011.08a
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    • pp.93-93
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    • 2011
  • 고진공 펌프 국산화의 일환으로 터보분자 펌프와 크라이오 펌프 개발이 진행 중이다. 크라이오 펌프 개발은 기계연구원, 우성진공(주), 국민대학교가 연합해서 수행하고 있다. 올해 9월말에 끝나는 1단계 마지막 3차 년도에는 두 고진공 펌프 모두 시제품을 완성하고 성능을 입증해야 한다. 이를 위해 고진공 펌프 개발과 별도로 진공 펌프 종합특성평가 시스템 개발도 표준과학연구원 주도로 진행되고 있는데 크라이오 펌프 평가 시스템은 원자력연구원이 담당하고 있다. 완성된 크라이오 펌프 성능평가 장치는 상온 기준 5${\times}$10-11 mbar의 기저압력을 보이고 있어서 초고진공 영역에서 크라이오 펌프 운전성능을 평가할 수 있는 준비를 마쳤다. 현재 크라이오 펌프 냉동기는 전반적으로 목표 설계치에 근접한 냉각성능을 나타내고 있는데 예를 들면 2차 냉각단 냉각능력이 10 K, 10 W로 대형 크라이오 펌프를 제작하기에도 충분하다. 활성탄 어레이도 여러 모델들을 자작하여 배기성능을 시험해 보았으므로, 최종적으로 3,600 L/s 급에 적합한 어레이를 만들고 이를 개발된 냉동기에 얹고 열차폐와 몸통을 씌워 펌프로서의 운전성능을 평가하는 일이 남아있다. 시제품은 상용품과 달리 기기가 차지하는 공간이 크고 부대설비가 복잡해서 운전성능 평가 장치에 부착해서 실험하기 어려울 수도 있으므로 기계연구원 현장에서 진공 게이지와 기체도입구만 부착한 마구리 플랜지를 펌프 흡기구 위에 달고 간이로 배기속도를 측정하는 것도 고려하고 있다. 이 경우는 표준용기를 사용할 때보다 배기속도가 과대평가되므로 이를 보정해 주는 방안을 마련해 놓아야 한다.

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Analysis of Fixed Bed Reactor for the synthesis of DME from METHANE (천연가스를 이용한 DME 합성 고정층 촉매 반응기 해석)

  • Yoon En Sup;Lee Shin Beom;Ahn Sung Joon;Cho Byoung Hak;Cho Won Il;Baek Young Soon;Park Dal Keun
    • Journal of the Korean Institute of Gas
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    • v.8 no.4 s.25
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    • pp.42-49
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    • 2004
  • We study on and simulate the behavior of one-step fixed bed reactor which synthesize DiMethylEther(DME) from Methane. At last, we know that reaction is decreased in case of excess and no cooling because the temperature of reactor is decreased or increased seriously. Also, we study on optimizing the reactor so that we know the optimized operation condition according to cooling effect, space velocity of reactant and temperature of reactant, etc.

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