• The Korean Journal of Nuclear Medicine
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• v.4 no.1
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• pp.11-18
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• 1970

호르몬의 면역학적(免疫學的) 측정법(測定法)은 내분비학적(內分泌學的)인 입장(立場)에서 가장 중요(重要)한 연구방법(硏究方法)의 하나이며, 또한 핵의학적(核醫學的) 입장(立場)에서도 방사성(放射性) 동위원소(同位元素)를 이용(利用)한 성체관내(誠體管內) 측정(測定)의 하나로 중요(重要)한 位置를 차지하고 있다. 이 방법(方法)의 일반원리(一般原理)는 방사성(放射性) 동위원소(同位元素)로 표지(標識)된 호르몬이 특정항체(特定抗體)에 대(對)하여 가역적(加逆的)으로 반응(反應)하고, 표지(標識)된 항원-항체(抗原-抗體) 복합체(複合體)를 형성(形成)하는데 근거(根據)를 두고 있다. 단일(單一) system내(內)에서 표지(標識)되지 않은 호르몬이 표지(標識)된 호르몬과 면역학적(免疫學的)으로, 특정항체(特定抗體)에 대(對)해 경합적(競合的)으로 작용(作用)하기 때문에, 실제적(實際的)으로는 일정량(一定量)의 표지(標識)된 호르몬과 일정량(一定量)의 항체(抗體)가 있는 조건(條件)하에서 미지성료(未知誠料)와 알려진 양(量)의 호르몬을 가진 표준(標準) 호르몬을 동시(同時)에 처리(處理)하여, 표준곡선(標準曲線)을 얻고, 여기에서 미지성료(未知誠料)의 흐르몬 양(量)을 추정(推定)하게 된다. 이때 경합적(競合的)으로 결합(結合)한 표지(標識)호르몬(bound form)과 결합(結合)하지 못한 표지(標識)호르몬(free form)을 분리(分離)하는 것이 필요(必要)하며, 전기영동법(電氣泳動法), 이중항체법(二重抗體法), solid phase법(法), 염석법(鹽析法) 및 효소(酵素)에 의한 단백소산법(消散法) 등이 이 목적(目的)을 위해 개발(開發)되어 있다. 이 중(中)에서 전기영동법(電氣泳動法)은 변성(變性)된 호르몬 및 유리(遊離)된 방사성옥소(放射性沃素)를 알 수 있는 특장(特長)이 있고, 이중항체법(二重抗體法) 등은 다수(多數)의 성료(誠料)를 처리하거나, 시간(時間)을 단축(短縮)시키는 이점(利點)이 있다 호르몬에 방사성(放射性) 동위원소(同位元素)를 부착(附着)시키는 데에는 $^{131}I$ 및 $^{125}I$가 주(主)로 쓰이나, 각기 장단점(長短點)이 있으며, 높은 방사성(放射性) 활성도(活性度)를 얻기 위해 chloramine-T 및 Na-metabisulfite를 사용하는 부착방법(附着方法)이 주(主)로 사용된다. 미량의 호르몬을 실제 측정(測定)하기 때문에 표지(標識)호르몬의 비방사능도(比放射能度)가 높을 필요성(必要性)이 있으며, 이 경우에는 방사능(放射能)에 의(依)한 흐르몬의 변성(變性)이 큰 문제점으로 등장하게 된다.

• Radiation Oncology Journal
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• v.6 no.2
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• pp.169-176
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• 1988

A retrospective study was performed on 15 patients with suprasellar germ cell tumors treated by megavoltage external beam irradiation between Feb. 1979 and Dec. 1985. Follow-up period of survivors was 30 to 91 months. Histologic diagnosis was obtained before radiation therapy in 10patients (9 germinomas and 1 mixed). Five patients were treated without histologic verification. In 9 patients with biopsy-proven germinomas radiation therapy was delivered to the craniospinal axis in 6, to the whole brain in 3. In 5 patients with mixed germ cell tumor or elevated tumor marker, irradiation was delievered to the craniospinal axis in 2, to the whole brain in 2, and to the primary site only in 1. Total doses ranged from 5,000 to 5,500 cGy to the primary site, 3,000 to 4,400 cGy to the whole brain, and 1,300 to 3,000 cGy to the spine. In these 14, local tumor was controlled and primary or spinal failure was not observed. One patient without elevated tumor marker was treated to the whole brain. The tumor was not controlled and he had spinal recurrence. Overall survival and disease-free survival rates were $86\%$ at 5 year. It is proven that radiation therapy is an effective treatment for suprasellar germ cell tumors. The neuroendocrinologic presentation, tumor marker status, early response to radiation measured on CT seem to be useful means for selecting patients for radiation therapy when tissue diagnosis is not available.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.11
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• pp.1193-1197
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• 2005

The low frequency noise below 200 Hz, including inaudible infra-sound, is known to affect human physiology ; circulation, respiration, nerve, endocrine, etc. Legislation has been introduced in several countries regarding evaluation guideline and measurement method of low frequency noise. In this work, low frequency characteristics of the Seoul subway transportation system was investigated in terms of the noise level and spectrum in the interior of running passenger car and the subway station. The interior sound pressure level of the passenger car was between 60 and 105 dB in the frequency range of $1{\sim}200\;Hz$ and varied with car speed. The marked sound pressure level peak at 8 Hz, infra-sound, observed for the most of Lines is shown to correspond to the resonance frequency of passenger car. The level of station platform noise was lower than the interior noise of running car because of the lower speed at arriving/departure. The results indicated that the interior noise level of running passenger car was inside the oppressive feeling region, proposed by Ochiai, in the frequency range of $20{\sim}80\;Hz$ which makes a little concern.

• Korean Journal of Animal Reproduction
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• v.23 no.3
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• pp.213-220
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• 1999

The litter size has been the primary interest of economic traits in pig reproduction. It has been recently shown that insulin-like growth factor-Ⅰ(IGF-Ⅰ) plays roles in establishing pregnancy and in supporting fetal growth and development. But, the effect of serum IGF-Ⅰ on litter size has not been studied. Therefore, this study was conducted to relate serum IGF-Ⅰ concentration with porcine litter size and to investigate the possible connection with estrogen receptor(ER) as a candidate gene for the litter size. Sera during day 45 to 105 of pregnancy were collected from two groups showing high and low litter size and serum IGF-Ⅰ concentration was measured by radioimmunoassay (RIA). IGF-Ⅰ levels in both groups decreased gradually as pregnancy stage proceeded but were not significantly different. Secondly, DNA was extracted from blood and PCR-RFLP was utilized to analyze ER genotypes of pigs in each group, which produced three polymorphic patterns. Based on the ER genotypes analyzed, low litter size group showed higher IGF-Ⅰ concentration than high litter size group. Taken together, the results indicate that the serum IGF system was correlated with steroid system but not with the litter size in pigs. Thus, this study implies that porcine litter size could be determined locally at the ovary level.

• Korean Journal of Environmental Biology
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• v.21 no.1
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• pp.18-25
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• 2003

Effects of tributyltin chloride (TBTC) in vitro on mixed function oxygenase (MFO) system on liver microsome of eight marine fish species were investigated. To determine the effects on MFO system, cytochrome P45O (CYP) and cytochrome b5 con-tents, activities of two reductases (NADH-cytochrome b5 reductase and NADPH-cy-tochrome P450 reductase) and four dealkylation enzymes (EROD, PROD, MROD and ECOD) were measured in fish microsoms exposed to TBTC for 20 min. The WP content was reduced to 10% of the control group in 6 out of 8 species exposed to TBTC, whereas there was no significant change in the cytochrome bs content. the response of NAD(P)H dependant reductases depended on fish species. The dealkylation enzyme activities in microsome were also apparently inhibited by TBTC. The degree of inhibition was different among fish species and four enzymes. The EROD activities in eight species were decreased to the range of 1∼65% of control group.

• Applied Microscopy
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• v.30 no.1
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• pp.73-80
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• 2000

Di-(2-ethylhexyl)phthalate (DEHP) has been known as one of endocrine disruptors. The present study was carried out to investigate the alterations of fine structure in rat seminal vesicle after oral intubation of DEHP in dosages of 1g/kg/day, 2g/kg/day or 3g/kg/day respectively in 0.5 ml of corn oil for If days. In rats treated with DEHP for 15 days, seminal vesicle exhibited extensive histological alterations compared to those observed in control groups. The size of the seminal vesicle and the mucosal folds decreased, but the lamina propria was considerably thickened. The ultrastructural changes of epithelial cells in seminal vesicle of rat treated with DEHP were characterized by the high nuclear/cytoplasmic ratio and the increased beterochromatin within irregular nuclear envelope. And also, the rough endoplasmic reticulum, Golgi complex and secretory vesicles were poorly developed. In conclusion, DEHP caused the ultrastructural and functional alterations of seminal vesicle in rats dose-dependently. It is suggested that these detrimental effects of DEHP on seminal vesicle are derived from the decrease level of testosterone.

• Science of Emotion and Sensibility
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• v.14 no.4
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• pp.531-536
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• 2011

The precise measurement of human emotion is of pivotal importance in the field of emotion science. Based on the perspective of psychoneuroimmunoendocrinology, human emotion is now considered to be measurable by body fluid. The biological molecule cortisol, which is known for the stress hormone, has been widely investigated to help researchers to estimate the stress loaded on human body. Until now, several measurement techniques such as High Performance Liquid Chromatography (HPLC), fluorometric assay, and reverse phase chromatography have been developed. However, since these measurements are expensive, take relatively long time for an operation, and they are not portable, they are not appropriate for POCT (point of care testing). In this paper we demonstrate the performance of a miniaturized-microwave resonant device in the measurement of cortisol. Our method has many advantages in that it requires a small volume of sample, has fast response time, is easy to operate and needs no labeling process. Besides, it will shed a light on the measurement techniques for emotion science.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.11
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• pp.454-458
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• 2017

Fibroadenoma are one of the most common benign tumors of the breast in young women. Fibroadenoma may be associated with fibrocystic change, proliferative epithelial changes, and extremely rarely with carcinoma. We report here two cases of malignancy arising from a breast fibroadenoma. The patients were 19 and 51 years old and presented with a lump of recent onset. A 19-year-old female patient was diagnosed with mass excision at another hospital, and re-excision was performed at the hospital. Ultrasonography and cytologic examination revealed fibroadenoma and malignancy in a 51-year-old female patient, who was treated with wide excision. The pathological report of the two cases was revealed as DCIS in a fibroadenoma. Because carcinoma arising within a fibroadenoma is so rare, there are few reports of its characteristics or guidelines for treatment. Careful analysis of the unusual carcinoma arising within a fibroadenoma of the breast led to appropriate diagnosis and treatment of various types of lesions. Herein, we report two cases of DCIS arising within a fibroadenoma of the breast and provide a review of the literature.

• Journal of Yeungnam Medical Science
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• v.10 no.1
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• pp.144-156
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• 1993

This study was performed to investigate the effect of octreotide on the contractility of rat vas deferens. The smooth muscle strips isolated from the prostatic portion were myographied in isolated organ bath, Electric field stimulation (monophasic square wave, duration: 1 mSec, voltage : 50 V, frequency : 5 Hz or 30 Hz, train: 10 Sec) produced reproducible contraction. The contraction was composed of two component, first phasic component (FPC) and second tonic component (STC). These contractions were abolished by tetrodotoxin ($1{\mu}M$). Octreotide inhibited the field stimulation induced contractions both FPC and STC concentration-dependently. The FPC was decreased by a desentization of purinergic receptor by pretreatment of mATP, and the STC was decreased by pretreatment of reserpine(3 mg/kg, IP) 24 hours before experiments. Octreotide reduced the field stimulation induced contraction in the presence of mATP and of reserpinized muscle strips. The inhibitory effect of octreotide was more potent at 5 Hz than at 30 Hz. Octreotide did not affect basal ton and exogenous norepinephrine- or ATP-induced contraction. These results suggest that octreotide inhibit the contractility of the isolated rat vas deferens by inhibition of the release of neurotransmitters, both ATP and norepinephrine from adrenergic nerve terminal.

• Environmental Analysis Health and Toxicology
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• v.22 no.4
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• pp.321-327
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• 2007

4-nonylphenol (4-NP)이 해산어류인 조피볼락, Sebastes schlegeli의 혈장 vitellogenin (VTG), alkaline-labile protein phosphorus (ALPP), calcium(Ca), glutamate pyruvate transaminase (GPT) 및 hepatosomatic index (HSI)에 미치는 영향을 조사하였다. 실험어에 3일간격으로 $estradiol-17{\beta}$ ($E_2$, 5 mg/kg B.W.) 또는 4-NP(0, 10, 50, 100및 200 mg/kg B.W.)을 복강에 2번 주사한 후, 7일째에 채혈과 적출을 통해 혈장과 간장을 수집해 분석이 실시되었다. 대조 실험어에는 용매로 사용된 70% 에탄올만이 투여되었다. $E_2$ 투여 실험어의 혈장 단백질을 전기 영동상으로 분석한 결과 약 170 kDa의 위치에서 짙은 VTG 밴드가 관찰되었으나, 용매만 투여한 대조 실험어의 혈장에서는 동일 밴드가 관찰되지 않았다. 4-NP 투여한 모든 실험어의 혈장 단백질에서는 $E_2$ 투여 실험어와 동일한 VTG 밴드가 관찰되었다. 혈장 ALPP와 Ca 농도도 4-NP 투여 실험어에서 $E_2$ 투여 실험어와 유사하게 증가하였으며, 이들 농도 변화는 VTG 합성과 더불어 증가하는 경향을 나타냈다. 혈장 전위효소인 GPT와 HSI도 $E_2$ 투여 실험어와 유사하게 4-NP가 투여된 모든 실험어에서 급격히 증가하였다. 이상의 결과로부터 연안생태계 내에서 서식하는 어류가 4-NP과 같은 내분비 장애물질(Endocrine Disrupting compounds, EDCs)에 의해 영향을 받는지를 규명하기 위한 생물학적 지표로서 VTG와 더불어 혈장 ALPP와 Ca이 사용가능 할 것으로 판단된다. 또한, 조피볼락과 같은 해산어가 EDCs에 노출되어 VTG가 합성될 때 간장 기능의 손상으로 혈장 전위효소인 GPT가 일시적으로 종가하고 간장도 비대해져 HSI가 높아지는 것으로 판단된다.