• Food Science of Animal Resources
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• v.26 no.4
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• pp.517-524
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• 2006

We investigated the effect of different kinds of dietary fats on the fatty acid composition, cholesterol content and quality of hens' eggs. The Haugh units, breaking strength, shell thickness and yolk color were not significantly different among the test groups. The type of dietary fat fed each test group notably altered the polyunsaturated fatty acid composition and cholesterol content of egg yolk. The n-6 fatty acid content of egg yolk was highest in the corn oil fed group being 31.61%, and this tended to decrease in the tallow, linseed oil and fish oil fed groups(P<0.05) in that order. The n-3 fatty acid content of egg yolk tended to increase in the linseed oil and fish oil fed groups with values of 9.74% and 5.16%, respectively(p<0.05). The increase of n-3 fatty acids in the yolk resulted in a reduced cholesterol content of the eggs. The cholesterol content ranged from $15.98{\sim}18.37mg/g$ of yolk or $227{\sim}261mg/60g$ of egg(p<0.05). The cholesterol content of egg yolk was highest in the tallow fed group, and significantly reduced to 13.01% in the fish oil group, 11.49% in the linseed oil group and 6.91% in the corn oil group(p<0.05). This result suggests that it is possible to reduce the cholesterol content or to increase the n-6 and n-3 polyunsaturated fatty acid contents in eggs by the manipulation of dietary fats.

• Korean Journal of Poultry Science
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• v.41 no.1
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• pp.1-5
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• 2014

This study was carried out to investigate the effect of feeding fermented earthworm cast additives (FEC) on egg production, egg yolk fatty acids, blood lipid, cecal microorganism and fecal odor. The completely randomized experiment was applied, and the treatments were designed as 2 feeding regimens (control vs. FEC feeding), and FEC contained 3.5% top dressing to common diet. Egg production and egg weight of FEC group were significantly higher than that of control (p<0.05). Ratios of n-6/n-3 fatty acids in the egg yolk of FEC group were significantly higher than those in control group (p<0.05). Concentrations of HDL-C in FEC group was significantly higher than control group (p<0.05), but not triglyceride and total cholesterol. The caecal Lactobacillus of FEC group was significantly higher in FEC group than control group (p<0.05). $NH_3$ concentrations from poultry house were significantly lower in FEC group than control (p<0.05). In conclusion, these study results showed that the addition of FEC at 3.5% to the diet has reduced odor of poultry house and improved the n-6/n-3 fatty acid of egg yolk and egg production in laying hens.

• Korean Journal of Poultry Science
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• v.30 no.3
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• pp.161-167
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• 2003

A study was conducted to investigate the effect of supplementing earthworm meal (EWM) on the performance of laying hens and fatty acid composition in egg yolks. A total of 360 laying hens at 55 weeks of age were fed the experimental diets containing 0.0% (Control), 0.1% and 0.2% of EWM for 5 weeks. Eggs were collected and weighted every day and egg production and feed conversion were recorded every weeks during the experimental period. However fatty acid composition of egg yolk were measured at last week of experimental period. Amount of feed intake tended to increase by supplemental EMW, but feed conversion ratio of birds fed EWM was not different among three groups. Average egg production seemed to increase and significantly improved (P<0.05) when fed a 0.1% EWM and 0.2% EWM, respectively. Average egg weight was prone to decrease when fed a 0.1% EWM compared to that fed a 0% (control) or 0.2% EWM. Average daily egg mass tended to improve by the addition of EWM. It was more increased in 0.2% EWM treatment than 0.1% EWM. The ratio of egg yolk n-6/ n-3 fatty acids contents was 5:1 fed a 0.1% and 0.2% EWM. But these ratio was 10:1 in control group. It is concluded that 0.2% earthworm meal supplementation in the 55 weeks old laying hens diet, improves the laying performance and ratio of egg yolk n-6/ n-3 fatty acids contents (P<0.05).

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.103-104
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• 2004

This study was evaluated the dietary affect of ceramics on laying performance, intestinal microflora population, NH$_3$ and VFA gas emission from excreta and fatty acids composition of egg yolk in laying hens. A total of 180 layer at 34 weeks of age were fed the experimental diets containing 0.0 % (Control), 0.4 % and 0.8 % of ceramics powder for 6 weeks. It is concluded that 0.4 % ceramics powder supplementation in the 34 to 40 weeks laying hens diet, improves the laying performance and ratio of egg yolk n-6/n-3 fatty acids contents and decreasing emission of NH$_3$ and VFA gas from excreta.

• Korean Journal of Ichthyology
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• v.33 no.3
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• pp.167-176
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• 2021

This study was carried out to clarify the egg, larvae and juveniles development of Abbottina springeri. For the experiments, the matured adults were collected at the Seowon-cheon, Dangjin-si, Chungcheongnam-do, in Korea. The amount of spawning of female A. springeri was about 1,225~2,100 (1,662±437, n=10). The fertilized eggs were circular in shape and 1.05~1.13 (1.08±0.02, n=30) mm in diameter. The hatching time was required 72 hours to 80 hours after fertilization under water temperature of 22℃. The newly hatched larvae were 2.10~2.23 (2.16±0.04, n=10) mm in total length and had egg yolk in the abdomen but the mouth was not opened. At 5 days after hatching, the preflexion larvae were 3.19~3.30 (3.24±0.03, n=10) mm in total length and the most of yolk-sac was absorbed. At the 15 days after hatching, the flexion larvae were 4.97~5.30 (5.13±0.12, n=10) mm in total length and the tip of the caudal fin was began to bend upward. At the 25 days after hatching, the postflexion larvae were 8.97~9.60 (9.44±0.16, n=10) mm in total length and the tip of the caudal fin was bent at 45°. At the 35 days after hatching, the juvenile were 12.0~13.5 (12.7±0.53, n=10) mm in total length and all fin-rays (iii7 dorsal fin, iii6 anal fin, i7 ventral fins) were reached a constant number of each part.

• Korean Journal of Poultry Science
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• v.32 no.1
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• pp.35-41
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• 2005

The objectives of the present study were to investigate the effects of varying levels of dietary oil containing conjugated linoleic acid (CLA) on the egg production and fatty acid composition of egg yolk. Seventy-two 59-wk-old ISA Brown laying hens were randomly allotted to four dietary treatments, each consisting of three replicates with six birds per replicate. There were four treatments that consist of diets containing 0, 1, 2, or $3\%$ commercial CLA-containing oil. Egg production was not significantly different among the dietary treatments at 0, 2, 4, and 6 week. The proportion of saturated fatty acids such as C16:0 and C18:0 in egg yolk were increased, but that of monounsaturated fatty acid C18:1 was decreased by feeding CLA-containing oil supplementation. However, the proportion of polyunsaturated fatty acids such as C18:2 and C18:3 in egg yolk were not different among dietary treatments at 2 and 4 wk of the experiment. At 6 week, the proportion of C18:2 in egg yolk was decreased by feeding CLA-containing oil compared with the control. Polyunsaturated fatty acid:saturated fatty acid (P:S) ratio and n-6:n-3 polyunsaturated fatty acid ratio were similar across the treatments between 2 and 4 week. The P:S ratio was decreased by dietary CLA-containing oil supplementation at 6 week. The proportion of CLA in egg yolk was linearly increased with increasing levels of CLA-containing oil supplementation. In conclusion, dietary supplementation of CLA-containing oil to laying hens increased beneficially increased CLA content in their egg yolk.

• Korean Journal of Ichthyology
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• v.35 no.4
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• pp.253-262
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• 2023

This study compared the results of observing the early life history of Gymnogobius urotaenia, which lives in Hwangbocheon Stream, an inflow stream on the east coast, with the differences between fish of the same Gobiidae fishes. In May 2022 and 2023, fertilized eggs and brood stork scattered under rocks were captured twice in Hwangbocheon Stream. The spawning amount was 827~1,540 and the orchid was a elliptical in shape with a size of 3.21×1.07 mm. The stage of ovulation observed in the laboratory was 16 cells, and hatching began after 193 hours. The breeding water temperature range was 18.8~19.3℃. Newly after hatching larvae, the yolk sac with a total length of 3.84~4.33 (average 4.10±0.17, n=30) mm, and the anus was not open. 6 days after hatching of incubation, the total length was 5.32~6.11 (average 5.67±0.25, n=30) mm, absorbing all egg yolk and transitioning to the preflexion larvae, ingesting food, and developing a keynote on the tail fin. 15 days after hatching, the end of the urostyle end was completely bent at 45° with a total length of 7.33~8.52 (average 7.81±0.46, n=30) mm and transitioned to postflexion larvae, and melanophore developed throughout the body. 38 days after hatching, the total length is 22.1~26.1 (23.8±1.36, n=30) mm and the number of fins (6 first dorsal fins, 11 second dorsal fins, and 11 anal fins, 12 ventral fins) is all the number of fin base became integer and transferred to the juvenile. As a result of the study, it was possible to distinguish the melanophore of postflexion larvae of G. urotaenia from other postflexion larvae Gobiidae fish in that they were distributed throughout the body of half the body and tail.

• Korean Journal of Ichthyology
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• v.33 no.2
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• pp.57-63
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• 2021

This study was carried out to clarify the egg, larvae and juveniles development of Carassius cuvieri in Nakdong River, Gyeongbuk. The amount of spawning of female C. cuvieri was about 30,400~44,900 (average 37,650±7,250). The egg shape was circular and the size was 1.24~1.37 mm (average 1.31±0.04 mm, n=30). At 100 hours after fertilization, more than of the total embryos were hatched. The newly hatched larvae had an 4.69~5.65 mm in total length (average 5.15±0.31 mm, n=10) and had egg yolk in the abdomen. At 3 days after hatching, the preflexion larvae absorbed all egg yolk was 6.27~6.70 mm in total length (average 6.59±0.08 mm, n=10). On the 10 days after hatching, the postflexion larvae were 8.71~8.92 mm in total length (average 8.81±0.07 mm, n=10), and the tip of the caudal fin was bent at 45°. On the 42 days after hatching, the total length of 15.1~16.8 mm (average 15.8±0.57 mm, n=10) was transferred to juvenile as the number of fins was (iii17 dorsal fins, iii4 anal fins) reached a constant number of each part.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.2
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• pp.184-188
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• 2002

The fatty acid contents of egg yolk from various chicken eggs such as general egg, ginseng egg, gamgoal egg and docosahexaenoic acid (DHA) egg were analysed during storage at 4$^{\circ}C$ for 3 weeks. The major fatty acids of all egg yolk oils were oleic acid (18 : 1) and palmitic acid (16 : 0). The contents of both fatty acids reached maximum at the first or second week and decreased at the third week. DHA was detected from 4 kinds of eggs stored for 2 or 3 weeks. The n-6/n-3 ratio was obtained under recommended intake range in fresh gamgoal egg and in general and DHA eggs stored far 0~3 weeks. For 3 weeks, polyunsaturated fatty acid / monounsaturated fatty acid/ saturated fatty acid (P/M/S) ratio of DHA e99 did not chance, but that of ginseng e99 gradually increased. The P/M/S ratio was generally maintained under recommended intake range in general egg stored for 3 weeks, in ginseng egg stored for 2 weeks and in DHA egg during all the storage period.

• Korean Journal of Ichthyology
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• v.13 no.3
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• pp.181-189
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• 2001

Spawning behavior of the Takifugu pardarlis (Temminck et Schlegel) was observed on the Jook-do coast in Tongyong from March 1997 to June 1999. The spawning ground was locted in the intertidal zone between Tongyong and Koje-do. Its bottom was mainly gravels and stones, and its depth was 0.5~1.0 m. Spawning season was from the end of the March to the middle of May. During the spawning season, the mature fishes formed school a of 10~30 individuals, then moved to the spawning ground together. When a mature female spawned eggs, the attendant males fertilized them at the same time. The fertilized eggs obtained from the parent fishes caught at the spawning ground were adhesive, opaque and spherical, measuring 1.14~1.24 mm (mean 1.19 mm, n = 50) in diameter with numerous tiny oil globules. Hatching period was about 205 hours after fertilization at water temperature of $18.0{\pm}0.5^{\circ}C$. The newly hatched larvae were 2.92~3.10 mm (mean 3.01 mm, n = 20) in total length (TL), had a large yolk, and 11~13+14~15 = 25~28 myomeres. At 5 days, the larvae had attained 3.79~3.85 mm (mean 3.82 mm, n = 20) in TL and had transformed into the postlarval stage. At 15 days, the postlarvae had attained 7.78~7.90 mm (mean 7.84 mm, n = 20) in TL. At 21 days, had larvae attained 10.15~10.27 mm (mean 10.21 mm, n = 20) in TL and had reached the juvenile stage. All fins were formed with a complete set of fin rays having the following counts: dorsal fin rays 11~12; anal fin rays 9; pectoral fin rays 14~15; caudal fin rays 11~12.