• Proceedings of the Malacological Society of Korea Conference
• /
• 2005.07a
• /
• pp.36-36
• /
• 2005

• Clinical and Experimental Reproductive Medicine
• /
• v.11 no.2
• /
• pp.41-49
• /
• 1984

It has been well known that ovarian steroids, estradiol and progesterone play an important role in the endometrial preparation for the implantation process. The present investigation was undertaken to correlate function of ovarian steroids with cAMP concentrations in uterine tissues of rat during the various stages of the preimplantation period. Rats ovariectomized on day 2 were treated with estradiol or/and progesterone on day 3 or on day 4 and cAMP concentrations in uterine tissues were determined by competitive protein - binding assay in control - and steroid treated - ovariectomized rats. The results obtained are as follows; 1. In control rats, cAMP concentrations in uterine tissues were decreased with preimplantation period proceeded whereas cAMP concentrations were increased and showed the highest levels on day 6 in ovariectomized rats. 2. In rats treated with progesterone only or progesterone with estradiol after ovariectomy, cAMP concentrations on day 6 were lower than those of ovariectomized control but higher than those of intact control rats while estradiol only treatment decreased cAMP concentrations on day 3 and day 6, compared with those from intact- and ovariectomized-controls. It is, therefore, concluded that the concentrations of cAMP in uterine tissues were lower in estrogen-treated rats than in ovariectomized rats, suggesting the involvement of cAMP in the regulation of uterine tissue differentiation.

• Nuclear Medicine and Molecular Imaging
• /
• v.42 no.3
• /
• pp.209-217
• /
• 2008

Purpose: To date, anatomical imaging modalities of the pelvis and tumor markers have been the mainstay of surveillance for recurrent ovary cancer. This study aimed to assess the role of $^{18}F$-FDG PET/CT in evaluation of ovary cancer recurrences, especially in comparison with enhanced a and tumor marker CA 125. Materials and methods: 73 patients who had PET/CT scan for restaging of confirmed ovary cancer, and additional imaging with enhanced a of the pelvis within one month were included. CA 125 level was available in all patients. From the PET/CT images, maximum standard uptake values (SUVmax) of suspected recurrence sites were recorded. Confirmation was available through re-operation or biopsy in 26 cases, and clinical assessment with series of follow-up images in 47. Results: PET/CT had 93% sensitivity and 88% specificity for detecting recurrent ovary cancer. Enhanced a of pelvis had sensitivity and specificity of 83% and 88%, and CA 125 50% and 95%. Conclusion: PET/CT has higher sensitivity for detecting recurrent ovary cancer compared to enhanced a though the differences were not significant. PET/CT has significantly higher sensitivity than CA 125. However, the three tests all agreed in only 43% of the recurrence cases, and recurrence should be suspected when any of the tests, especially PET/CT, show positive findings.

• Journal of Life Science
• /
• v.27 no.1
• /
• pp.1-7
• /
• 2017

Abnormal actin remodeling is a typical characteristic of tumor cells. Thymosin ${\beta}_{10}$ (TB10) and profilin-1 (PFN-1) are actin-binding proteins and essential regulators of actin polymerization. We previously showed that TB10 induced death in ovarian cancer cells by sequestering F-actin, but the underlying mechanisms of this induction have not been explored. In this study, we identified TB10 as a novel regulator of PFN-1 and demonstrated its novel function as a tumor suppressor in ovarian cancer cell lines. The present study investigated protein expression profiles through polyacrylamide gel electrophoresis (PAGE) and liquid chromatography-mass spectroscopy (LC-MS/MS) in SKOV3 cells, an ovarian cancer cell line, that were transiently transfected with TB10. PFN-1 was highly overexpressed in response to TB10, and overexpression of PFN-1 resulted in inhibition of cell proliferation and migration and promotion of cellular apoptosis in ovarian cancer cells. Furthermore, transiently transfected PFN-1 appeared to deactivate the Erk signaling pathway, followed by decreased expression of Elk-1 and Egr-1 in human ovarian cancer cells. Interestingly, PFN-1 did not affect the activation of Akt. The results demonstrated that PFN-1 induced apoptotic cell death and inhibited proliferation and migration in ovarian cancer cells, suggesting that PFN-1 may be valuable in anti-cancer therapy.

• Journal of Life Science
• /
• v.28 no.6
• /
• pp.733-737
• /
• 2018

Cucurbitacin-I, a natural triterpenoid derived from Cucurbitaceae family plants, exhibits a number of potentially useful pharmacological and biological activities. Indeed, the previous study demonstrated that cucurbitacin-I reduced the proliferation of colon cancer cells by enhancing apoptosis and causing cell cycle arrest at the G2/M phase. CD44, a type I transmembrane protein with the function of adhering to cells, mediates between the extracellular matrix and other cells through hyaluronic acid. Recent studies have demonstrated that an overexpression of the CD44 membrane receptor results in tumor initiation and growth, specific behaviors of cancer stem cells, the development of drug resistance, and metastasis. The aim was to examine the effect of cucurbitacin-I on CD44 expression human ovarian cancer cells because the effect of cucurbitacin-I on CD44 expression has not been reported. The expressions of CD44 mRNA and protein were detected using a quantitative real-time reverse-transcription polymerase chain reaction and a Western blot analysis, respectively. Treatment with cucurbitacin-I inhibited the expression of CD44 mRNA and protein. A subsequent analysis revealed that cucurbitacin-I blocked the phosphorylation of activator protein-1 (AP-1) and nuclear factor kappa-B ($NF-{\kappa}B$), which are key regulators of CD44 expression. Taken together, the data demonstrate that cucurbitacin-I regulates the AP-1 and $NF-{\kappa}B$ signaling pathways, leading to decreased CD44 expression.

• Journal of Animal Science and Technology
• /
• v.46 no.3
• /
• pp.315-324
• /
• 2004

Cytochrome P450 aromatase is the enzyme responsible for biosynthesis of female sex hormone(estrogen) and 19-nortestosterone(nandrolone), a unique steroid hormone endogenously synthesized in the pig. By use of RT-PCR coupled with DHPLC technique (WAVE analysis), expression pattern of isoforms of porcine cytochrome P450 aromatase gene was investigated. Relatively higher expression of aromatase mRNA was observed in testis than in ovary and this result accounted for the previous findings of higher blood estrogen level in male compared with female in this species. The result from the DHPLC demonstrated that PCR amplified DNA fragments of ovary and testis tissues. using unique PCR primers for all three types of aromatase genes, were different from those of type II and ill genes. Further nucleotide sequence analyses of the plasmid clones containing the PCR products revealed that nucleotide sequences of all clones were identical to type I aromatase gene(ovary type). Thus, the result from the present study indicates that the ovary and testis express the same type of aromatase gene. Therefore, the efficacy of DHPLC techniques used for this study helped us to analyze tissue-specific expression of isoform of genes containing the nucleotide sequences with high homology.

• Journal of Embryo Transfer
• /
• v.22 no.3
• /
• pp.191-194
• /
• 2007

The purpose of the present study was to set up basic information of size and status of ovaries by using ultrasonography to retrieve in vivo matured oocytes with ovum pick- up method. Ovaries were collected from the abattoir in Jeju in May and June which is breeding season. When the size of ovaries on ultrasonography was compared with real size measured by caliper, no significant difference was shown (p<0.05). The number of preovulatory follicles (>21mm) was investigated with ultrasonography and naked eyes. Ultrasonography group had 0.83 preovulatory follicles per ovary and naked-eye group had 0.75 preovulatory follicle per ovary and their average size was 2.86 cm and 2.34 cm, respectively. The average number of follicle was 4.25 with ultrasonography and 4.38 with naked eyes. There was no significant difference considering the size of follicle and number of follicle between ultrasonography and actual size except for the size of preovulatory follicle, suggesting that information of ultrasonography is able to use for OPU or other reproductive technology of mare.

• Korean Journal of Clinical Laboratory Science
• /
• v.48 no.1
• /
• pp.8-14
• /
• 2016

Granulosa cells surround the oocyte within the ovarian follicle and play an essential role in creating conditions required for oocyte as well as follicular development. The current study was conducted to examine the gene expression profile of mouse ovaries during the primordial to primary follicle transition process. Total RNAs from mouse ovaries on day 5 and day 12 were synthesized cDNA using annealing control primers. The DEGs were cloned and their identities were analyzed by BLAST search. The Plekha5 and Anxa11 were highly expressed in primary follicle stage. By contrast, their expression was increased in granulosa cells at the primary follicle stage. We have successfully discovered a list of genes expressed in day 5 and day 12 ovaries and confirmed that some of them are differentially expressed in PMF and/or PRI. This is a spatial-temporal regulatory mechanism on the ovarian folliculogenesis through membrane fusion. The gene expression profile from the current study would provide insight for future study on the mechanism(s) involved in primordial-primary follicular transition. This will provide information for identification of the mechanism of ovarian dysfunction.

• The Journal of the Korea Contents Association
• /
• v.15 no.9
• /
• pp.599-605
• /
• 2015

This study was conducted to evaluate suppressive effects of black rice aleurone layer extract (BRE) on body fat, serum lipid, and hormone levels in ovariectomized rats. The rats were divided into five groups (n=8/group): sham operated group (S); ovariectomized control group (OVX); ovariectomized group treated with isoflavone 10 mg/kg B.W. (IF); ovariectomized group treated with BRE 30 or 90mg/kg B.W. (BRE-30 or -90). High fat diet (45% calories from fat) was fed to all rats for 12 weeks. Body weight gain, body fat weight, and adiposity index increased in the OVX group, but they significantly decreased in IF or BRE supplementation. Serum triacylglycerol and leptin levels decreased in BRE groups while serum adiponectin level significantly increased compared to that of the OVX group. Hepatic total lipid, triglyceride, and cholesterol levels increased in the OVX group, but they decreased in BRE supplementation (p>0.05). These data suggest that black rice aleurone layer may be a useful food source to decrease obesity and its related diseases by modulating lipid metabolism in estrogen-deficiency model.

• Journal of Aquaculture
• /
• v.9 no.1
• /
• pp.73-81
• /
• 1996

This study was conducted to study the reproductive cycle in female fusilier, Caesio diagramma by using the histological methods. Histological changes of the ovary were well correlated with the patterns of gonadosomatic index (GSI) and hepatosomatic index (HSI). GSI was increased in April as the value of HSI increase and reached to its maximum in May and June. Oocytes at the chromatin-nucleolus and peri-nucleolus stages were observed in the ovary throughout the year. In April, oocytes containing yolk appeared in ovaries of a few fishes. Most oocytes appearing in May and June belonged to the tertiary yolk stage. Frequency of oocytes appearance at the tertiary yolk stage in May and June was higher than that of the other months. Moreover, the empty follicles and atretic oocytes were observed in the ovaries with many vitellogenic oocytes during these two months. Thereafter, oocytes of the yolk stage disappeared in September. The spawning period of C. diagramma is from the month of April to June, and this species belongs to an asynchronous and multiple spawner.