• Korean Journal of Plant Tissue Culture
• /
• v.28 no.1
• /
• pp.7-13
• /
• 2001

Calli were induced from diploid and haploid tobacco after 4 weeks and maintained on MS medium with combination of 2.0 mg/L 2,4-D,0.1 mg/L BAP and 2.0 mg/L kinetin. Suspension cells were screened through 65 $\mu$m-nylon mesh and 100 $\mu$m-mesh, then they were smeared on selection medium combined with cadmium and PFP by using the low melting agarose of 0.8%. After 30days smeared cultures of the medium the cell was treated with 500 $\mu$M and 1000 $\mu$M to select the resistant cell line were selected. Plant regeneration was induced from the selected cell lines on medium with 0.5, 1.5, 2.0 mg/L BAP and on media with combination of auxin and BAP under 500 $\mu$M and 1000 $\mu$M cadmium. At this time, plant regeneration was achived on cadmium free medium. In case of haploid, occurred from the cell line which is selected in medium with cadmium and PFP. In case of diploid regeneration occurred is in the medium with cadmium alone. The plantlet regenerated from cadmium resistant calli grew well in cadmium 500 $\mu$M. Protein pattern of leaf, root, stem of regenerated plants was analyzed. The quantum was 6.5188 ug/mg.fr.wt in the leaf of plant, 5.3611 ug/mg.fr.wt in the stem, 3.0213 ug/mg.fr.wt in the root. On the other hand, 5.9652 ug/mg.fr.wt. in the leaf of control, 3.5974 ug/mg.fr.wt in the stem of the control, 4.3766 ug/mg.fr.wt. in the root of the control. The one dimension bends regenerated from cadmium resistant calli resistant to cadmium in leaf were 49 involving 198.7KD etc. Disappeared were 4 involving 160.5KD etc, The protein bends were combinized were 3 involving 83.4KD etc. The bends resistant to cadmium stress in stem were 41 involving 4.3KD etc. Disappeared were 5 involving 114.8KD etc. The protein bends combinized were 6 involving 128.7KD etc. The bends which had the resistance to cadmium stress in root is 27 in volving 166,9KD etc. The bends which disappeared were 198.7KD etc. There were 5 involving 83.4KD etc.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.34 no.3
• /
• pp.254-259
• /
• 2001

In order to make clear the resistance of plane nettings u,sed widely in constructing net cages, the resistance R taken by $R=kSU^2$, where S was the wall area of nettings, U the flow velocity, and k the resistance coefficient, was measured in a circulating water channel by using nylon Raschel nettings and PE trawler-knotted nettings coated with anti-fouling paint or not and then the properties of coefficient k were investigated. The mesh size L and the angle $\phi$ between two adjacent bars was given by the function of Reynolds number ${\lambda}U/v$ in the region of ${\lambda}U/v<180$, i. e., $$k=350(\frac{\lambda U}{v})^{-0.25}$$.where $\lambda$ was the representative size of nettings expressed as $$\lambda=\frac{{\pi}d^2}{2L\;sin\;2{\phi}}$$On the other hand, the coefficient k was almost fixed between 92 and 102 ($kg{\cdot}s^2/m^4$) in the region of ${\lambda}U/v{\geq}180$ and varied according to the ratio $S_n/S$ of the total area $S_n$ of nettings projected to the plane perpendicular to the water flow to the wall area S of nettings, i.e., it was given by $$k=98.6(\frac{S_n}{S})^{1.19}$$ regardless of the coating of paint.