• The Korea Beekeeping Bulletin
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• s.330
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• pp.20-23
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• 2008

저번 호에는 꿀벌봉군붕괴현상(CCD)의 원인들에 대하여 고찰하였습니다. 미국, 호주 그리고 유럽국가에서 보고된 내용을 보면 벌들의 탈출현상은 매우 다양한 이유에 의해서 생겨났음을 여러분도 쉽게 이해하셨을 것입니다. 한창 많은 벌들이 벌통 안에서 식구를 늘려가야 제때에 채밀에 성공할 수 있는데 벌이 사라져 없으니 기막힌 사실이 된 것입니다. 냉정한 마음으로 깊이 생각할 필요가 있다고 봅니다. 다행이 우리나라는 미국, 유럽처럼 아직 심각한 정도는 아닙니다. 그러나 우리나라에서도 진행되고 있다는 사실이 나타나고 있다고 말씀드린 바 있습니다. 1992년 중국에서 꿀벌이 수입 되었을 때 처음으로 중국가시응애가 묻어와 우리 양봉산업이 위기를 맞기도 하였습니다. 조사된 피해수준은 전체 봉군의 30~70%가 붕괴되었습니다. 꿀벌응애와 중국가시응애, 부저병, 백묵병과 노제마병은 전국 어느 곳 양봉장에서도 발생하였습니다. 좁은 벌통 안에서 여러 가지 질병이 연속적으로 꿀벌을 괴롭힌 것입니다. 미국의 경우 화분매개용 봉군은 개화시기에 맞추어 먼 곳까지 이동해야 하므로 벌은 스트레스를 받는다고 합니다. 스트레스가 원인이 되어 꿀벌의 붕괴가 생겨났다는 주장인데 많은 분들이 그럴 것으로 수긍하고 있습니다. 이와 같은 스트레스는 위에서 언급한 응애와 여러 질병이 발생하여 꿀벌이 겪어야 하는 고통에 비교하면 훨씬 낮은 스트레스라 생각합니다.

• The Korean Journal of Pesticide Science
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• v.12 no.2
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• pp.192-196
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• 2008

This study examined the acaricidal activity of a new compound, K16776 against honeybee mite, Varroa destructor which is ecto-parasite of Apis mellifera. Acaricidal activity was performed using six acaricides, two Chinese commercial acaricides and one newly synthesized K16776 against V. destructor in the small container and in the bee hive. K16776 and amitraz exhibited 100% acaricidal activity against V. destructor without insecticidal toxicity to A. mellifera in the small container. The other acaricide was not activity. Applied to the bee hives, K16776 showed acaricidal activity as 98.7% ($250{\times}$) and 88.6% ($500{\times}$) and amitraz showed as 100% ($500{\times}$) and 90% ($1,000{\times}$), respectively. Made in China, Cao Suan Sha Man Pian and Wangs showed acaricidal activity as 56.9% and 66.7%, respectively. The result indicates that K16776 can be potentially useful control agent against honeybee mite, V. destructor.

• The Korea Beekeeping Bulletin
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• s.282
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• pp.20-20
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• 2004

• The Korea Beekeeping Bulletin
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• s.295
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• pp.39-40
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• 2005

• Proceedings of the Korea Information Processing Society Conference
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• 2023.05a
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• pp.399-401
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• 2023

최근 꿀벌 집단 실종이 잇따라 발생하고 있으며 이에 대한 뚜렷한 원인은 없으나 꿀벌응애류, 말벌류에 의한 폐사와 이상기후가 복합적으로 작용했기 때문이라고 추측하고 있다. 추측하고 있는 원인 중 꿀벌 봉군의 약화 요인으로 작용하는 등검은말벌의 분포도를 시각화하여 꿀벌의 개체 수 피해를 줄이는 데 도움이 되고자 한다.

• Journal of Apiculture
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• v.34 no.1
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• pp.15-26
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• 2019

Tracheal mite (Acarapis woodi) is an internal parasite that is parasitic on the bronchus of adult bees and sucks fluid from the trachea. Since its first report by Rennie, it has been spread throughout Europe and in some Asian regions, with adjacent Japan and China reported in 2011 and 2012, respectively. Korea detected specific genes of A. woodi in 2015, but only one of 99 samples has been identified and the being of A. woodi has not been confirmed. In this study, we established a specific nested PCR method to confirm for detecting low-copy number of A. woodi-specific gene in bee samples. As a result, A. woodi-specific COI gene was amplified in 15 of 23 samples, and they were judged positive by melting point analysis and sequencing analysis. Although we could not observe the existence of the mites in bees, our results suggest that tracheal mit might exist in nature.

• 동물약계
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• no.21
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• pp.5-5
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• 1995

1. 제조(수입)업허가$\cdot$변경 2. 가축전염병 발생정보 3. 축산관계관 회의 4. 안전축산물 생산을 위한 종합설명회 제 1회 수의정책 심포지움 개최 6. 가축질병 병성감정 실시기관 지정 7. 동물용의약품 사용기준 시안 작성 제출 8. 세계수의학대회 개최 9. 재해예방 특별점검 안내 10. 생체내 유해잔류물질 검사킷트 조사 11. 꿀벌 가시응애구제제 기술전수 교육 12. 임시이사회 개최

• Journal of Veterinary Clinics
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• v.12 no.1
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• pp.887-896
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• 1995

In order to determine the efficacy of $Bayvarol^{(R)}$ strips(Bayer AG) against the mite Varroa jacobsoni and the safety of $Bayvarol^{(R)}$ strips in honey bees, a series of trials was carried out under field condition in autumn and spring. The results obtained were as follows; 1. The average varroacidal rate of $Bayvarol^{(R)}$ strips was 92.7% in autumn and 93.7% in spring. However the average varroacidal rats of treated control group was 73.8 % in autumn and 75.1 % in spring, and that of untreated control group was 12.1 % in autumn and 16.1 % in spring. 3. The average number of dead bees in $Bayvarol^{(R)}$ group was 13.0 in recommended dosage(RD), 12.3 in 2 times of RD and 14.0 in 4 times of 70 in autumn, and 19.7 in RD,17.0 in 2 times of RD and 19.0 in 4 times of RD in spring. However the average number of dead bees in treated control groups was 38.3 in RD, 62.7 in 2 times of RD and 60.3 in 4 times of RD in autumn and 18.0 in RD, 43.3 in 2 times of RD and 102 in 4 times of RD in spring and that of untreated control of group was 12.7 in autumn and 19.3 in spring. 3. The varroacidal effects and the number of dead bees of all groups were higher in spring than in autumn. 4. It was revealed that $Bayvarol^{(R)}$ were highly saft and effective varroacides for honey bees.

• Journal of Apiculture
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• v.34 no.1
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• pp.27-37
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• 2019

Rapid detection of Tropilaelaps, an external parasite of honeybees that lead to malformation of honeybee or colony collapse disorder, is becoming important. But it is very difficult to find with the naked eye of Tropilaelaps. In this study, we have developed a method to detect the specific gene of Tropilaelaps from the hive debris and to know the number of Tropilaelaps in the hive through Tropilaelaps-specific quantitative detection. Tropilaelaps-specific gene amplified in DNA extracted from hive debris by consecutive PCR (1st detection, 2nd nested PCR). It could detect 101 molecules level of Tropilaelaps-specific gene and confirm the amplification of the Tropilaelaps-specific gene. It was possible to accurately quantify the number of Tropilaelaps from the hive debris sample, which is difficult to discriminate the presence of Tropilaelaps visually, through Tropilaelaps-specific detection. Under the microscope, Tropilaelaps was collected and quantitative detection of Tropilaelaps-specific genes was performed. It was possible to quantify the number of Tropilaelaps present in the hive through the molecules of the quantified Tropilaelaps-specific genes. We suggest that hive debris can represent as a micro-environment to hive and show that it can be a simpler and more accurate sample than using a parasitic host honeybee. We expect that hive debris should facilitate the monitoring of Tropilaelaps in hive.

• Korean Journal of Microbiology
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• v.49 no.3
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• pp.270-274
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• 2013

The bumblebee, Bombus terrestris, has played an important role as one of the alternative pollinators since the outbreak of honeybee collapse disorder. Recently, pathogens and parasites such as viruses, bacteria and mites, which affect the life span and fecundity of their host, have been discovered in B. terristris. In order to detect the microsporidian pathogen, Nosema spp. in the field populations of B. terristris, we collected adults and isolated their genomic DNA for diagnostic PCR. The PCR primers specific for Nosema spp. were newly designed and applied to gene amplification for cloning. Only small subunit ribosomal RNA (SSU rRNA) gene of N. ceranae was successfully amplified among examined genes and sequenced, which indicates that N. ceranae mainly infects the examined field population of B. terristris. To detect of SSU rRNA gene, two regions of SSU rRNA gene were selected by primary PCR analysis and further analyzed in quantitative real-time PCR (qRT-PCR). The qRT-PCR analysis demonstrated that SSU rRNA of N. ceranae was detected at concentration as low as $0.85ng/{\mu}l$ genomic DNA. This result suggests that the detection via qRT-PCR can be applied for the rapid and sensitive diagnosis of N. ceranae infection in the field population as well as risk assessment of B. terristris.