• Current Research on Agriculture and Life Sciences
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• v.32 no.4
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• pp.211-214
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• 2014

This study investigated the effects of using a pollen suspension for artificial pollination on the labor costs, fruit set, and fruit quality attributes of 'Fuji' apples. The pollen germination rate was 20% in a 20% fructose solution after 6 hours, and the pollen remained stable for 6 hours in the same solution. The king fruit per flower cluster exhibited a normal fruit set, regardless of the treatment. Plus, none of the artificial pollination applications affected the fruit length/diameter ratio, flesh firmness, soluble solids concentration, or titratable acidity in the harvested 'Fuji' apple fruits. However, the labor savings were significantly higher with the pollen suspension treatment when compared with the cotton swab or love-touch. Therefore, the labor cost was four-fold less in use of pollen suspension than in use of cotton swab for artificial pollination.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.235-242
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• 2015

Morphology and characteristics of floral organ in highbush blueberry cultivars were studied to select suitable cultivars of highbush blueberry for domestic cultivation. The stamen consists an anther and a tape-like hairy filament with well-developed trichomes. When the anther was opened, the wall of anther was not dehiscent, and pollen grains were discharged into two tubes. Pollen was mature tetrad type without being separated after meiosis (Late March). The number of pollen granules per anther was 400~1,300, the germination rate was higher in the cultivars having many pollen grains. Pistil was composed of five carpels and a shipper without separate part. The number of ovules per ovary was 39~67, therefore, the coefficient of ranged from 11.6 to 31.0%. The seed pod formation by combination of ‘Bluejay’ and ‘Sharpblue’ was higher in the cross-pollination than in the self-pollination.

• Atmosphere
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• v.29 no.1
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• pp.1-12
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• 2019

For the allergy patient who needs to know the situation about the extent of pollen risk, the National Institute of Meteorological Sciences developed a pollen forecasting system based on the Community Multiscale Air Quality Modeling (CMAQ). In the old system, pollen emission from the oak was estimated just based on the airborne concentration and meteorology factors, resulted in high uncertainty. For improving the quality of current pollen forecasting system, therefore the estimation of pollen emission is now corrected based on the observation of pollen emission at the oak forest to better reflect the real emission pattern. In this study, the performance of the previous (NIMS2014) and current (NIMS2016) model system was compared using observed oak pollen concentration. Daily pollen concentrations and emissions were simulated in pollen season 2016 and accuracy of onset and end of pollen season were evaluated. In the NIMS2014 model, pollen season was longer than actual pollen season; The simulated pollen season started 6 days earlier and finished 13.25 days later than the actual pollen season. The NIMS2016 model, however, the simulated pollen season started only 1.83 days later, and finished 0.25 days later than the actual pollen season, showing the improvement to predict the temporal range of pollen events. Also, the NIMS2016 model shows better performance for the prediction of pollen concentration, while there is a still large uncertainty to capture the maximum pollen concentration at the target site. Continuous efforts to correct these problems will be required in the future.

• Annual Conference of KIPS
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• 2011.11a
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• pp.1031-1034
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• 2011

헬스케어에서의 상황정보는 사용자와 관련된 정보를 추론하여 질 높은 서비스를 제공하기 위해서 사용자가 필요로 하는 능동적이고 지능적인 서비스를 제공하여야 한다. 본 논문에서는 상황인식을 이용한 보건기상지수 모니터링 방법론을 제안한다. 체온, 기온, 조도, 습도, 자외선에 따른 건강지수를 사용자의 현재 위치에 따라 실시간으로 제공하기 위해서, GPS와 기상청의 RSS로부터 추출한 XML를 활용한다. 보건기상지수는 천식지수, 뇌졸중지수, 피부질환지수, 폐질환지수, 꽃가루농도지수, 도시고온지수의 요소에 따라 분석하여 모니터링한다. 상황정보 수집과 추론 과정을 통해 장치간의 유동성을 보장하는 환경에서 서비스를 지원하기 위한 도메인 상황정보를 구성한다. 이기종 디바이스의 유동성이 보장되는 환경에서 새로운 상황이 존재하면 추가된 상황정보의 서비스를 지원하기 위해서 Naive Bayes 분류자를 이용한다. 상황정보 수집, 상황인식 추론, 상황정보 모델링에 따른 새로운 상황 분류하는 방법론에 대해서 논리적 타당성과 유효성을 검증한다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2023.11a
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• pp.23-26
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• 2023

In the past, in observing the sea fog on the major sea route and providing real-time information for the safe operation of ships, a visibility sensor or a fog detector with similar operating principles was installed to observe local fog near the place where it was installed. However, it was somewhat unreasonable to immediately provide sea fog observation information to ships and users because the reliability of real-time observation information was somewhat low due to pollution caused by dust, salt, and pollen, or malfunctions of detection sensors by organisms such as spider webs. From 2019 to 2022, the Korea Meteorological Administration and the Ministry of Oceans and Fisheries collaborated to build a more reliable real-time image-based sea fog observation system in 100 regions of the Lighthouse on major sea routes across the country to collect reliable sea fog observation information every 10 minutes and perform real-time public service(webpage).

• Horticultural Science & Technology
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• v.29 no.6
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• pp.645-650
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• 2011

Pear cultivar 'Supergold' (Pyrus pyrifolia var. culta Nakai) was originated from the cross between 'Chuwhangbae' and 'Manpungbae' with the aims of improving the fruit quality of 'Chuwhangbae' cultivar at Pear Research Station of National Institute of Horticultural & Herbal Science, Rural Development Administration in 1994. 'Supergold' was preliminarily selected in 2002 and named in 2008. The tree shows a vigorous growth habit and semi-spread characters like as 'Manpungbae'. Furthermore, it has sufficient flowers and carries abundant pollen grains, so it can also be used as a pollinator. 'Supergold' is highly resistant to black leaf spot (Alternaria kikuchiana) in the field condition. The optimum harvest time is around Sep. 11th, which is ahead of 'Whangkeumbae' about 5 days in the harvest period. The fruit shape is oblate and fruit skin color is greenish-white at harvesting time. The average weight of fruit is 570 g, and the soluble solids content is $13.6\;^{\circ}Brix$. The flesh is very soft and juicy, and renders good eating quality. Shelf life is about 6 months under the cold storage condition. To determine the self-incompatibility (SI) genotype of 'Supergold' pear cultivar, it was crossed with other cultivars of which SI genotypes have already known. The result of cross-pollinations of 'Supergold' with other cultivars showed relatively high rates of fruit set from 64.5% to 91.0%, except for the cross with pollens of 'Nijisseiki' that represented only 28.8% of fruiting rate. Although sometimes the stigma of 'Supergold' crossed with 'Hayatama', 'Chojuro', and 'Nijisseiki' showed malformed pollen tube tips, 'Supergold' is generally supposed to have cross-compatibility with all other pollen donor cultivars. It is considered that the S-allele of 'Supergold' is $S_3S_4$, which is based on the result of PCR-RFLP.

• Journal of Bio-Environment Control
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• v.33 no.3
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• pp.148-155
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• 2024

This study aimed to assess the characteristics of fruit quality and seed formation resulting from pollination by four different pollen donors: 'SKK2', 'Deliwoong', 'Pohwa', and 'Chieftain'. The objective was to identify the most effective pollen donor for yielding commercially viable fruits in two cultivars of kiwifruit (Actinidia chinensis var. chinensis): 'Sunfl' and 'Garmhwang'. Over a two-year period, the fruit setting rate for 'Sunfl' and 'Garmhwang' kiwifruit, pollinated by the four donors, consistently exceeded 96%. For 'Sunfl', the heaviest fruit weight was observed when pollinated with 'Deliwoong' for both years, while the lightest fruit was associated with 'Pohwa' in 2021 and 'Chieftain' in 2022. For 'Garmhwang', there was a yearly variation with the heaviest fruit weight linked to 'Deliwoong' in 2021 and 'Chieftain' in 2022. When examining the fruit characteristics of 'Sunfl', the dry matter content was highest when pollinated by 'SKK2', and the soluble solids content peaked with 'Chieftain' in 2021, although no significant differences were observed in 2022. Total and immature seed numbers in 'Sunfl' were highest with hexaploid pollen donors, while the 1000-seed weight was highest with tetraploid pollen donors. For the fruit characteristics of 'Garmhwang', the dry matter content and titratable acidity were highest when pollinated by 'SKK2'. Soluble solids content showed annual variation. Mature and total seed number in 'Garmhwang' were highest with 'Deliwoong' and 'Chieftain', while the immature seed number was lowest with 'SKK2'.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.305-311
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• 2014

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world. Seed distribution and transgenes to environmental ecosystem is one of the most important factors in risk assessment and risk management of GM crop. Safe management for the development and commercialization of GM crops is required according to The Act on Transboundary Movements of Living Modified Organisms,etc (LMO Act) in Korea. This study was conducted to setup the environmental monitoring system of GM zoysiagrass (event JG21 and JG21-MS). The monitoring was performed in 4 GMO confined fields, Sungwhan, Ochang, Jeju University and Jeju Namwon. In the result of monitoring, we could not found any gene flow and distribution of GM zoysiagrass in the 3 fields, but one spill of JG21 was found in the Namwon field in 2012. These results suggest that continuous monitoring is necessary to detect the occurrence of GM zoysiagrass for preventing genetic contamination in natural environment.

• Journal of Life Science
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• v.30 no.3
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• pp.239-249
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• 2020

Persimmon flowers are fruit-bearing female, pollen-bearing male, or hermaphrodite, containing both a pistil and a stamen. Using prominent PCNA persimmons as male parents is very important for breeding programs, as the selection procedure for new cultivars bearing male flowers requires a long time and a large field in a traditional crossbreeding method. To improve breeding efficiency through early selection of male flower-bearing plants at the seedling stage, analysis was performed on 88 major cultivars whose gender expressions are known, using two male flower selection markers recommended by Akagi et al. The OGI locus marker and DISx-AF4S marker results showed that 83 and 72 cultivars, respectively, matched in terms of gender expression and marker analysis. For the OGI locus marker, 890 plants were selected from 2,509 seedlings obtained from crossbreeding with the mother plant "Migamjosang," which was the breeding cultivar. Comparing the gender expression of the flowers and the marker with 1,186 crossbred seedlings, excluding the unfertilized and dead plants, inconsistencies were found in 401 plants (33.8%). For the DISx-AF4S marker, 636 plants were selected from 889 seedlings obtained from 12 cross-combinations. The results of the sex expression and marker analysis were compared to 379 plants, excluding the unfertilized and dead plants, and inconsistencies were found in 247 plants (65.2%). These results indicate that the examined DISx-AF4S and OGI locus markers would not be suitable for utilization in the breeding field.

• Horticultural Science & Technology
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• v.17 no.4
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• pp.473-475
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• 1999

This study was carried out to restore the fertility by suppression of male sterility-induced gene using an antisense construct. Tobacco (cv. Petit Havana SR1) was transformed with the binary vector containing a GBAN215-6 promoter, an antisense diphtheria toxin (DTx-A) gene (pKDA215b) and a hygromycin resistant gene. Seventy-six confirmed transgenic plants regenerated from leaf disks were designated as the $R_0$ generation and selfed to produce the $R_1$ generation. From the inheritance study, five $R_1$ lines with multiple copies of the antisense construct were selected and selfed to identify homozygosity for the antisense construct. In order to restore fertility and finally to select restore lines, five $R_2$ lines with multiple copies of the antisense construct were crossed with male sterile plants. From these crosses, three different phenotypes have been observed: completely restored, partially restored, and not restored pollens, and otherwise tobacco plants were phenotypically same as normal plants. These plants were scored for the degree of restoration and selected for further study.