• Title/Summary/Keyword: 김정수

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Morphological Development of Egg and Larvae of Squalidus japonicus coreanus (Gobioninae) (몰개(Squalidus japonicus coreanus)의 난 발생 및 자치어의 형태 발달)

  • Kim, Kyeong-Mu;Kwak, Yeong-Ho;Kim, Seung-Yong;Song, Ha-Yun
    • Korean Journal of Ichthyology
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    • v.33 no.4
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    • pp.252-261
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    • 2021
  • The egg development and early life history of short barbel gudgeon Squalidus japonicus coreanus were investigated. For the experiments, the mature adults were collected at the stream Jicheon in Korea. The eggs from the females were obtain by injecting 10 IU/g of human chorionic gonadotropin and inseminated by wet method in the laboratory. The fertilized eggs were 1.12±0.03 mm (1.10~1.16 mm, n=30) in diameter. The embryo began to hatch about 49 hrs after fertilization under water temperature of 23±1℃. The newly-hatched larvae (Yolksac larva) were 3.7±0.1 mm (3.4~3.8 mm, n=16) in total length, and they haven't Melanophore. 5 days after hatching, the Preflexion larva were 5.3±0.2 mm (5.0~5.5 mm, n=16) in total length, and they began to eat a Rotifer. 19 days after hathing, the Flexion larva were 6.0±0.3 mm (5.4~6.5 mm, n=16) in total length, and they began to eat a Brine shrimp. 29 days after hatching, the Postflexion larva were 9.6±0.5 mm (8.3~10.5 mm, n=16) in total length, and dorsal fin rays are were formed. 44 days after hatching, the juvenile were 15.5±1.0 mm (13.5~17.0 mm, n=16) in total length, and all their fin-rays were formed.

The Complete Mitochondrial Genome and Molecular Phylogeny of the Flathead Platycephalus cultellatus Richardson, 1846 from Vietnam (Teleostei; Scorpaeniformes) (베트남 Platycephalus cultellatus Richardson, 1846 (Teleostei; Scorpaeniformes)의 전장 미토콘드리아 유전체와 분자계통)

  • Tran, Biet Thanh;Nguyen, Tu Van;Choi, Youn Hee;Kim, Keun-Yong;Heo, Jung Soo;Kim, Keun-Sik;Ryu, Jung-Hwa;Kim, Kyeong Mi;Yoon, Moongeun
    • Korean Journal of Ichthyology
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    • v.33 no.4
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    • pp.217-225
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    • 2021
  • The family Platycephalidae is a taxonomic group of economically important demersal flathead fishes that predominantly occupy tropical or temperate estuaries and coastal environments of the Indo-Pacific oceans and the Mediterranean Sea. In this study, we for the first time analyzed the complete mitochondrial genome (mitogenome) of the flathead Platycephalus cultellatus Richardson, 1846 from Vietnam by Next Generation Sequencing method. Its mitogenome was 16,641 bp in total length, comprising 13 protein-coding genes (PCGs), two ribosomal RNA genes, and 22 transfer RNA genes. The gene composition and order of the mitogenome were identical to those of typical vertebrates. The phylogenetic trees were reconstructed based on the concatenated nucleotide sequence matrix of 13 PCGs and the partial sequence of a DNA barcoding marker, cox1 in order to determine its molecular phylogenetic position among the order Scorpaeniformes. The phylogenetic result revealed that P. cultellatus formed a monophyletic group with species belonging to the same family and consistently clustered with one nominal species, P. indicus, and two Platycephalus sp. specimens. Besides, the cox1 tree confirmed the taxonomic validity of our specimen by forming a monophyletic clade with its conspecific specimens. The mitogenome of P. cultellatus analyzed in this study will contribute valuable information for further study on taxonomy and phylogeny of flatheads.

Effects of Fermented Oyster Extract Supplementation on Body Composition, Muscular Strengths and Blood Muscle Growth Fact ors in Elderly Women (발효굴 섭취가 중년 여성의 신체조성, 근력 및 혈중 근성장 인자에 미치는 영향)

  • Park, Joung-Hyun;Kim, Dong-Seok;Lee, Bae-Jin;Her, Jung-Soo;Jeon, Byeong Hwan
    • Journal of Marine Bioscience and Biotechnology
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    • v.13 no.2
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    • pp.76-85
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    • 2021
  • Crassostrea gigas were fermented using L. brevis BJ20 to prepare fermented oyster extract (FO). The participants of this study were randomly assigned to FO and placebo (CON) groups. The FO group was given 1.0 g of FO supplementation and the CON group was given sucrose each day for eight weeks. The effects of FO supplementation on body composition, muscula r strength, and blood factors associated with muscle growth were assessed. The FO supplement was enriched with arginine (6,183.3 mg), phenylalanine (217.9 mg), leucine (122.6 mg), isoleucine (59.8 mg), valine (16.4 mg), and γ-amino butyric acid (GABA, 1,053.7 mg). The total fat was significantly decreased in the FO group compared with the CON group (p < 0.05). 60D/S Ext.T/Wo rk and 60D/S Flex.T/Work concomitantly with 60D/S Flex.PeakTQ/BW were significantly increase d by FO treatment compared to CON group (p < 0.05). However, posture stability was not significa ntly different between the groups. The levels of angiotensin-converting enzyme were significantly decreased within the FO group (p < 0.05). The FO group showed significantly decreased levels of tumor necrosis factor-α and increased levels of human growth hormone compared with the CON group (p < 0.01). The levels of insulin-like growth factor-1 increased (p < 0.01) in the FO group while that of creatine kinase and triglyceride decreased significantly compared with the CON group (p < 0.05). These results demonstrated that FO supplementation is effective in preventing sarcopenic obesity and maintaining and strengthening muscular function in elderly wom en. Hence, FO supplements can be used as functional ingredients for these benefits.

Morphological Development of Egg and Larvae of Hemiculter leucisculus (치리(Hemiculter leucisculus)의 난 발생 및 자치어 형태 발달)

  • Kim, Kyeong-Mu;Kim, Seung-Yong;Song, Mi-Young;Song, Ha-Yun
    • Korean Journal of Ichthyology
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    • v.32 no.4
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    • pp.222-231
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    • 2020
  • The egg development and early life history of Sharpbelly Hemiculter leucisculus were investigated. For the experiments, the mature adults were collected at the Lake Yedang in Korea. The eggs from the females were obtained by injecting 10 IU/g of human chorionic gonadotropin and inseminated by wet method in the laboratory. The fertilized eggs were 0.97±0.02 mm (0.9~1.0 mm n=30) in diameter. The embryo began to hatch about 32 hrs after fertilization under water temperature of 22±1℃. The newly-hatched larvae were 3.0±0.2 mm (2.6~3.4 mm, n=15) in total length, and they haven't Melanophore. Six days after hatching, the Preflexion larva were 5.7±0.1 mm (5.4~5.8 mm, n=15) in total length, and they began to eat a Rotifer. 17 days after hatching, the Flexion larva were 6.8±0.2 mm (6.5~7.0 mm, n=15) in total length, and a gas bladder develop above the intestine. 30 days after hatching, the Postflexion larva were 8.8±0.7 mm (7.9~10.3 mm, n=15) in total length, three dorsal fin rays began to develop in the membrane fins. 50 days after hatching, the Juvenile were 20.8±0.8 mm (18.8~24.6 mm, n=15) in total length, and all their fin-rays were formed.

Flower Arrangement Study of Pine : Focusing on Pine Gracious Flower (소나무(松)의 화예적(華藝的) 고찰(考察) -송(松)의 문인화(文人花)를 중심(中心)으로-)

  • Kim, Jeong Min
    • Journal of the Korean Society of Floral Art and Design
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    • no.42
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    • pp.63-96
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    • 2020
  • The flower arrangement that human beings interact with the natural plants have characteristics according to their historical background and ethnicity and different aspects of culture related of the flower arrangements have been created by human inspiration and formative ability. The Korean flower arrangement is a product of civilization which originated in the natural environment of the four seasons. As background with the natural unity ideology, the Korean Flower Arrangement makes the life and philosophy of the naturalistic nature that flows in the mind of our nation to the relaxing beauty suited on cultures of ages by the Virtue, the Will, and the Vitality. The Literary Flower Arrangement is, based on the higher human personality and poetic sentiments as ideologies of our traditional oriental philosophy, the formative art made for aesthetic implications of philosophy by consisting plants' characters and tastes without expression of technical skills. In this study, focused on pine tree, in order to clarify that the flower arrangement of our spiritual culture is the Literary Flower Arrangement, make Identifying aesthetic characteristics through, the backgrounds and sources of being expressed by the Literary Flower Arrangement, poetic feelings, symbolism, metaphor, the formative form of flower arrangement, decoding of the flower arrangement genes, and the same time deduce the record of literary work and classify it each era, and establish the Literary Flower Arrangement theory which is the essence of Korean flower arrangement.

Analysis of Slurry Composting and Biofiltration Liquid Fertilization on the Initial Growth of Chamaecyparis obtusa (SCB액비 처리가 편백의 초기생장에 미치는 효과 분석)

  • Sang Hyun, Lee;Kwang Soo, Lee;Su Young, Jung;Hyun Soo, Kim
    • Journal of Korean Society of Forest Science
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    • v.111 no.4
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    • pp.594-602
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    • 2022
  • The study aim was to analyze the effects of slurry composting and biofiltration (SCB) liquid fertilization on the early growth of Chamaecyparis obtusa. Control, chemical fertilizer (CF), low liquid fertilizer (LLF), and high liquid fertilizer (HLF) sites with five trees per site were established, and each treatment was repeated three times. The growth analysis showed that HLF-200 (treated with HLF 200%) had the highest growth. To assess the fertilization effect, root-collar diameter and height growth models were developed for the HLF-200 and control groups. We found that the Schumacher anamorphic and Schumacher polymorphic equations were best suited for the root-collar diameter growth models in the control and HLF-200 groups, respectively. For the height growth models, the Gompertz polymorphic equation was the most appropriate. From the growth curve generated by the chosen model, the effect of fertilization on the amount and rate of the root-collar diameter and height growth were higher in the HLF-200 group than in the control group. Treatment with SCB liquid fertilization was judged to be suitable for the early growth stage of Chamaecyparis obtusa.

Effects of Plantaginis Semen & Poria (CJB) Water Extract on Histomorphometrical Aspects and Histopathological Changes in High Fat Diet-induced Obese Rats (차전자·복령의 복합 물추출물(CJB)이 고지방사료로 유도된 비만 생쥐의 조직형태 및 조직병리학적 변화에 미치는 영향)

  • Hwang, Jeong Soo;Suk, Jang Mi;Choi, Hye min;Shin, Insoon;Hwang, Su Jung;Park, Ji Young;Kim, Sung Ok;Seo, Bu Il;Kim, Mi Ryeo
    • The Korea Journal of Herbology
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    • v.28 no.1
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    • pp.91-96
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    • 2013
  • Objectives : Obesity is a chronic metabolic disease caused by disorder of energy balance and lipid metabolism. This study was conducted by histopathology and histomorphometry to investigate the anti-obesity effects of mixed water extract of Plantaginis Semen & Poria (CJB) on liver, epididymal fat pads and pancreas zymogen granules in obese rats induced with high fat diet. Method : Male Sprague-Dawley rats to be divided four groups were fed into four different treatments: normal (NOR) diet, high-fat (HF) diet, HF diet+CJB (100 mg/kg and 300 mg/kg, P.O.) for 8 weeks. The weekly body weights were measured in four experimental groups, respectively. Also histopathological and histomorphometrical changes of liver, epididymal fat pads and pancreas zymogen granules were observed in normal control and obese rats, respectively. Results : Adminstration of CJB significantly reduced body weights compared to those of HF group for experimental period. After 8 weeks, liver weights in the CJB groups were lower than those of HF group. In addition, HF diet related steatohepatitis, adipocyte hypertrophy, exocrine disturbances (decreases of pancreatic zymogen granules) were also dose-dependently inhibited by treatment of test material, CJB 100 and CJB 300 as compared with HF group, respectively. Conclusion : Based on the results, it is considered that CJB will be showed hepatoprotective and anti-obese effects, may be directly and/or indirectly mediated by pancreatic zymogen granules because they dose-dependently inhibited steatohepatitis, hypertrophy of adipocytes and decreases of pancreatic zymogen granules induced by HF diet supply, respectively.

A Review of Clinical Studies for Chinese Medicine Treatment of Idiopathic Thrombocytopenic Purpura Using the CNKI Database (특발성 혈소판 감소성 자반증의 중의치료에 대한 임상연구 동향 - CNKI검색을 중심으로)

  • Ji-eun Bae;Jae-won Park;Jun-kyu Lim;Mi-so Park;Jeong-su Hong;Dong-jin Kim
    • The Journal of Internal Korean Medicine
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    • v.43 no.6
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    • pp.1045-1062
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    • 2022
  • Objectives: The aim of this study was to analyze the latest clinical studies on Korean medicine treatment of idiopathic thrombocytopenic purpura (ITP) in the Chinese National Knowledge Infrastructure (CNKI) database. Methods: We searched the last 6 years of clinical studies discussing Oriental medicine-based treatments for ITP in the CNKI database. A meta-analysis of 13 RCTs was performed by synthesizing the outcomes, including the measured platelet count and total effective rate. The quality of the studies was assessed using Cochrane's risk of bias (RoB) tool. RevMan 5.4.1 software was used for data analysis. Results: Of the 15 selected studies, 1 was a non-randomized controlled trial (nRCT), 2 were case series, and 12 were randomized controlled trials (RCTs). Treatments in all studies included oral herbal medicine. The most frequently used herbal decoction was the Liangxue Jiedu prescription (凉血解毒方), and the most commonly used herb was Agrimonia pilosa (仙鶴草), Astragali Radix (黃芪), Fossilia Glycyrrhizae Radix et Rhizoma (甘草), and Rehmannia glutinosa Liboschitz ex Steudel (地黃). The meta-analysis showed significantly better improvement in platelet counts and total effective rate for ITP in the treatment group than in the control group. Conclusion: Treatment with herbal medicine was effective in treating ITP. However, the significance of this conclusion is somewhat limited due to the low quality of the available studies. Multifaceted and scientifically designed clinical studies are required to develop treatments for ITP based on Korean medicine. The results of this study could be used as basic data for further ITP studies.

Comparative Studies of Kriging Methods for Estimation of Geo-Layer Distribution of Songdo International City in Incheon (인천 송도국제도시 지층분포추정을 위한 크리깅 방법의 비교연구)

  • Kim, Dong-Hee;Ryu, Dong-Woo;Lee, Ju-Hyoung;Choi, In-Gul;Kim, Jong-Kook;Lee, Woo-Jin
    • Journal of the Korean Geotechnical Society
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    • v.26 no.5
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    • pp.57-64
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    • 2010
  • Kriging techniques have been used to estimate the spatial distribution of soil layers and soil properties in the geotechnical engineering area. Since the selected kriging technique may provide different values of estimation, the selection of method is important in the geotechnical estimation. In this paper, the spatial distribution of the thickness of consolidation layer of Songdo International City is estimated using simple, ordinary, and universal kriging techniques, and the reliability of estimated results is analyzed. It is shown that the consolidation layer thickness estimated by the simple kriging technique is larger than those by other kriging techniques when the location of estimation is far from the locations where the measured data exist. In this case, the reliability of the simple kriging technique is observed to be lower than those of other techniques. Universal kriging gives a negative value for thickness of consolidation layer in some locations away from the data. It is concluded that the ordinary kriging is the most optimized estimation technique because the reliability of ordinary kriging technique is higher than those of other ones and the consolidation layer thickness estimated by the ordinary kriging locates within the reasonable range.

Evaluation of Preconsolidation Stress Considering Small-Strain Shear Wave Velocity (미소변형 전단파 속도를 고려한 설행압밀하중 산정)

  • Yoon, Hyung-Koo;Lee, Chang-Ho;Kim, Joon-Han;Lee, Jong-Sub
    • Journal of the Korean Geotechnical Society
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    • v.25 no.5
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    • pp.5-16
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    • 2009
  • Preconsolidation stress is one of the important design parameters in soft soils because the behavior of saturated soft soils changes dramatically at the preconsolidation stress. For the estimation of preconsolidation stress, the global vertical settlement without considering micro strain behavior has been considered. The purpose of this study is to propose and verify a new method called the "shear wave velocity method" for determination of the preconsolidation stress reflecting on particle behavior at the small-strain. In this study, the undisturbed soft soils obtained at Busan, Incheon and Gwangyang in Korea were used. The oedometer cell incoporated with the bender elements is used for the consolidation tests under the $K_0$ condition. The preconsolidation stress determined by the proposed method is compared with that estimated by Casagrande (e-log p'), Sridharan (log (1+e)-log p'), and Onitsuka (In(1+e)-log p') methods. This study suggests that the shear wave velocity method may determine simply the preconsolidation stress with considering the small-strain behavior.