• Title/Summary/Keyword: 기초 분석

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Monitoring the Rate of Frozen Denaturation of Bovine Myosin by Competitive Indirect ELISA Method (Competitive Indirect ELISA를 이용한 Bovine Myosin의 동결 변성도 측정)

  • Kim, Seong-Bae;Lee, Ju-Woon;Park, Jong-Heum;Do, Hyung-Ki;Hyun, Chang-Kee;Shin, Heuyn-Kil
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.862-870
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    • 1998
  • This study shows the application of Ci-ELISA method for monitoring the denaturation of myosin by the frozen treatment in order to differentiate thawed beef from chilled. Hanwoo M.Semitendinosus (n=25) was treated under the two different frozen process as follows; simple frozen treatment (Exp-1) at 4 different temperatures, -10, -20, -50 and $-80^{\circ}C$, respectively, and repeated thawing-refreezing treatment (Exp-2) stored at 4 different temperatures, -10, -20, -50 and $-80^{\circ}C$, respectively. Antibodies (Abs) were produced from rabbits immunized with myosin whole molecule (MWM) isolated from beef round, heavy meromyosin S-1 (S-1) and light meromyosin (LMM) prepared by digestion of MWM. Each immunoglobulin G (IgG) was separated from antiserum. At 6 month storage, IA of anti-MWM IgG for myosin was decreased to 32.67, 32. 23, 51.52 and 34.27% in Exp-1 and to 14.82, 15.61, 25.3 and 23.7% in Exp-2 at -10, -20, -50 and $-80^{\circ}C$, respectively (P<0.05). In Exp-1, the reactivities of anti-LMM IgG were decreased to 25.12, 21.42, 49.05 and 28.96%, and those of Exp-2 were to 11.88, 9.56, 20.63 and 12.64% at -10, -20, -50 and $-80^{\circ}C$, respectively, at 6 times thawing (P<0.05). Conclusively, myosin was denaturated by freezing treatment and LMM or myosin rod part might have suffered from more extreme demage than HMM S-1, and samples at $-50^{\circ}C$ were slightly injured less than others by freezing treatment.

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Effects of the Glycoprotein Isolated from Pteridium aquilinum on the Immune Function of Mice (고사리 단백다당(Pteridium aquilinum Glycoprotein, PAG)이 마우스 면역활성에 미치는 영향)

  • Park, Hyeon-Ae;Kweon, Mee-Hyang;Han, Hyung-Mee;Sung, Ha-Chin;Yang, Han-Chul
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.976-982
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    • 1998
  • The effects of the glycoprotein (PAG) isolated from Pteridium aquilinum on the immune function was examined in mice. PAG was intraperitoneally administered into BALB/C mice for 14 days and the antibody forming ability to hen egg lysozyme (HEL) and the blastogenic responses of splenocytes were measured. PAG treatment significantly increased antibody formation to HEL in a dose-dependent manner. Blatogenesis of splenocytes in response to lipopolysaccharide (LPS, B-cell specific mitogen) or phytohemagglutinin (PHA, T-cell specific mitogen) was also increased after treatment with PAG, indicating that the PAG increases both humoral and cellular immunities. To examine whether the immune function of PAG was via a direct effect on the lymphocytes, splenocytes were isolated from BALB/C mice, exposed to various concentrations of PAG in vitro and the blastogenic responses were measured. In vitro exposure to PAG significantly increased blastogenesis of splenocytes to LPS up to $500{\;}{\mu}g/kg$, whereas the blastogenic response to PHA was not altered by PAG treatment. To identify the fraction responsible for the increase in the immune function, the effect of periodate digest, pronase digest or purified polysaccharide on the antibody production to HEL was examined. Crude protein fraction of PAG significantly increased the antibody formation to HEL. On the other hand, both crude and purified polysaccharide fractions did not have any effects on the antibody production ability. These data indicated that 1) PAG increased both humoral and cellular immune functions, 2) the increase in humoral immunity was probably via a direct action of PAG on lymphocytes and 3) the protein portion of PAG was responsible for the increase in humoral immunity.

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Comparison of Science Gifted and Ordinary Elementary School Students with Regard to the Concept of Groundwater (초등학교 과학영재학생과 일반학생의 지하수에 대한 개념 비교)

  • Lee, Hyong-Jae;Park, Sang-Tae
    • Journal of Gifted/Talented Education
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    • v.22 no.4
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    • pp.855-874
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    • 2012
  • This research aimed to obtain basic data for elementary school students to form proper concepts by comparing the science gifted students and the ordinary students of elementary school with regard to the groundwater concept, formation process, existence forms, and movement. The research subjects were 65 fifth and sixth graders of the elementary school students and the spatial ability test was conducted on the subjects, and 4 science gifted students and 8 ordinary students chosen from the subjects were analyzed using half-structured interview data and ground water drawing drawn by the students. The conclusion derived in accordance with the research purpose is summarized as follows. It was found that there were no great differences in the answers to the question asking what groundwater is between the science gifted elementary school students with high spatial ability and the ordinary elementary school students with moderate spatial ability. The ordinary students with low spatial ability tended to regard groundwater as the concept of water and sewage. In the concept of the formation process of groundwater, the science gifted elementary school students with high spatial ability explained it by citing diverse surface water such as rainfall, river water, lake, and waterfall, and the ordinary elementary school students with moderate spatial ability all mentioned only rainfall and river water and could not explain diverse spatial factors. The ordinary elementary school students with low spatial ability mentioned rainfall and river water and perceived that groundwater was formed artificially. In the concept regarding the existence form of groundwater, the ordinary elementary school students with low spatial ability could not think of space perception that small pore space exists in earth or soil in the ground. The science gifted elementary school students with high spatial ability knew that groundwater exists in pore space with regarding groundwater movement, the ordinary elementary school students with low spatial ability thought that there was no groundwater movement and that it could be moved only by artificial facilities. There were differences in the perception of pore space and in the perception of existence and non-existence of groundwater movement accordingly, but for most of the elementary school students, the concept of groundwater was formed differently from the scientific concept. It is considered that most of the elementary school students formed erroneous concept about groundwater and could not connect ground water under the surface of the earth with the substances forming its surroundings with regard to the concept of groundwater.

A Survey on the Consumer Attitude Toward Health Food in Korea (II) -Consumer Perception on Health Foods- (건강식품에 대한 소비자 인식 연구 (II) -건강식품에 관한 소비자 의식구조-)

  • Lee, Eun-Joo;Ro, Seung-Ok;Lee, Cherl-Ho
    • Journal of the Korean Society of Food Culture
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    • v.11 no.4
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    • pp.487-495
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    • 1996
  • The consumer perception on health and food habit, the experience of health food use and the discrimination between health food and drug of Korean consumer were surveyed by using a questionnaire containing 20 items in order to obtain the basic data for the assessment of the benefit and risk of health foods in Korea. A total of 1,000 people over 20 years of age living in Seoul and the vicinities were interviewed and asked to fill out the questionnaire during the period from the October 1995 to the February 1996. Among the 882 answers collected 23 was incomplete data, and 859 answers were used for the statistical analysis by using SAS program. The survey revealed a strong interest of the consumer on health food by showing that more than a half of the subjects (58.8%) had the experience of actual use of health food, and 68.2% believed the effectiveness. What the consumer expect most from health food was to have beneficial effect to maintain overall health condition (59.8%), and the most negative aspect of health food was the overstatement on the effectiveness by the producers (52.1%). The most important source of information for the purchase of health food was the suggestion of friends and relatives (30.6%). Among the health foods registered and regulated by the food law, royal jelly (22.7%), squalene (16.0%), refined fish oil (15.1%), lactic acid bacteria (10.6%) and aloe (8.8%) were relatively well aware. Although 84% of the subjects perceived that health food is different from drug or traditional medicine, the largest percentage of the subject selected ginseng as the most well known type of health food (22.7%) as well as the most well known drug (or traditional medicine) (41.7%). Ginseng was also chosen as the most frequently used health food (17.0%), and vitamin tablets the third (13.0%). The vague definition of health food and unambiguous discrimination of it from medicine by the consumers were problematic for the correct use and reasonable purchasing behavior. The clear definition and proper regulation on the manufacture and distribution of health food, more strict control of labelling and advertisement, and a wide consumer education on health food were recommended.

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유아를 위한 메뉴 개발 및 평가

  • 신은경;이연경
    • Proceedings of the KSCN Conference
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    • 2003.11a
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    • pp.1053-1054
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    • 2003
  • 유아기는 식품 기호도, 식사예절, 식습관 등 식행동의 기초가 형성되는 시기로서 특히 편식이 생기기 쉽고, 식욕부진과 영양과잉 등 불균형한 식품 섭취로 인한 영양문제를 초래할 수 있다. 본 연구에서는 2003년 7월 구미지역 2개 보육시설의 92명 유아들의 부모들을 대상으로 설문조사를 실시하여 유아들의 식습관과 식품 기호도를 조사하고 이를 고려하여 유아를 위한 메뉴를 개발하고 평가하였다. 개발한 메뉴는 일반식 (삼각김밥, 오징어전), 편식 (도라지강정, 브로콜리스프), 빈혈식(쇠간함박스테이크, 참치깻잎쌈밥), 비만식(참치샐러드, 닭안심깻잎조림), 전통식(떡찜, 두부선) 등 각 2가지씩 총 10가지이며, 2003년 10월 시식회를 거쳐 656명의 유아들에게 맛, 냄새, 모양, 조직감, 전반적인 기호도 등에 대해 5점 척도법으로 메뉴평가를 실시하였다. 식습관 조사결과 편식습관이 있는 경우가 70.7%였으며, 평소 싫어하는 음식에 대해서는 권유로 마지못해 먹는다가 67.4%로 가장 많았다. 성별에 따른 유아의 식습관을 분석해 본 결과 전반적으로 남녀간 유의한 차이는 없었으나 남아가 여아보다 탄산음료를 즐겨 마시는 것으로 나타났다(p<0.05). 기호도 조사결과 10가지 식품군 중 과일류에 대한 기호도(3.97$\pm$0.65점)가 가장 높았고, 채소류에 대한 기호도(2.46$\pm$0.68점)가 가장 낮았으며, 기호도가 특히 낮았던 식품으로는 곡류 및 감자류 중에는 보리밥과 콩밥, 육류 및 가공품 중에는 간과 내장, 두류 및 가공품 중에는 팥, 어패류 중에는 굴, 해조류 중에는 파래, 채소류 중에는 토란, 브로콜리, 도라지 등이었다. 조리법별로는 튀김류(3.80$\pm$0.68)가 가장 높았고 그 다음이 볶음ㆍ구이류(3.77$\pm$0.56)였으며 기호도가 가장 낮은 음식은 생채류(2.61$\pm$1.27)와 숙채류(2.85$\pm$1.13)인 것으로 나타났다. 개발한 메뉴의 선호도는 전반적으로 높았으며 맛에 대한 선호도(4.30$\pm$0.91)가 가장 높았고, 모양에 대한 선호도(3.95$\pm$0.89)가 가장 낮았다. 메뉴별 맛에 대한 선호도는 닭안심깻잎조림 (4.85$\pm$0.36)과 두부선(4.85$\pm$0.52)이 가장 높았고, 참치깻잎쌈밥(3.43$\pm$1.61)이 가장 낮았으며 냄새에 대한 선호도는 두부선(4.76$\pm$0.69), 닭안심깻잎조림 (4.64$\pm$0.53), 브로콜리스프(4.59$\pm$0.88)가 유의하게 높았고 참치깻잎쌈밥(2.95$\pm$1.24)은 유의하게 낮았다. 모양에 대한 선호도는 두부선이 4.85$\pm$0.52점으로 유의하게 높은 점수를 보였으며 쇠간함박스테이크(3.03$\pm$0.17)와 참치깻잎쌈밥(3.11$\pm$1.33)이 유의하게 낮은 점수를 보였다. 조직감에 대한 선호도는 두부선(4.80$\pm$0.56)과 브로콜리스프(4.63$\pm$0.60)가 유의하게 높게 나타났고, 참치깻잎쌈밥(3.20$\pm$1.29)이 유의하게 낮았다. 전반적인 선호도는 맛, 냄새, 모양, 조직감에서 모두 선호도가 높았던 두부선이 4.82$\pm$0.49점으로 가장 유의하게 높았으며 참치깻잎쌈밥이 3.17$\pm$1.30점으로 가장 낮게 나타났다. 음식의 맛, 냄새, 모양, 조직감 및 전반적인 기호도간에는 모두 유의한 상관관계가 있는 것으로 나타났다(p<0.01). 따라서 음식의 맛에 영향을 주는 요인은 맛 자체뿐만 아니라 냄새, 모양, 조직감 등 다양하므로 어릴 때부터 가정과 보육시설에서 다양한 식품과 조리법을 이용하여 맛있고, 먹고싶은 냄새가 나고, 부드러우며, 모양도 먹음직스럽게 조리하여 유아들의 올바른 식습관 형성에 도움이 되도록 하는 것이 필요할 것으로 사료된다.

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Studies on the Production of Alcohol Fermented Milk (알코올 발효유(醱酵乳)의 제조(製造)에 대(對)하여)

  • Yoo, Jin-Young;Kang, Tong-Sam;Min, Byong-Yong
    • Korean Journal of Food Science and Technology
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    • v.10 no.3
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    • pp.337-343
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    • 1978
  • In order to develope a new kind of fermented milk, basic studies on several lactic acid bacteria and yeasts were conducted, 8 kinds of alcohol fermented milk were manufactured and sensory evaluation was undertaken. The results obtained are summarized as follows: 1. Four kinds of lactic acid bacteria were isolated, among which Y-2 strain was strongest in acid productivity and it was elucidated that acid productivity of all strains was stornger in synthetic medium than in milk medium. 2. The pH in milk medium inoculated with Y-2 strain and incubated at $30^{\circ}C$ for 24 hours was dropped from 5.8 to 3.8 and fluctuation in amino nitrogen content was found during incubation. 3. The pH in milk medium inoculated with K. fragilis and incubated at $30^{\circ}C$ for 7 days was dropped from 6.2 to 5.2 and amino nitrogen content was in the range of $0.12{\sim}0.27mg/ml$. Alcohol productivity of K. fragilis was stronger than E-2 and E-4 strain but no difference in alcohol productivity was found between milk medium and synthetic medium. 4. The repression in growth and acid productivity of lactic acid bacteria was recongnized if inoculated after inoculating yeast firstly. 5. Alcohol productivity was increased rapidly at the end of acid production of lactic acid bacteria if lactic acid bacteria if lactic acid bacteria and yeast were inoculated simultaneously. 6. Sensory evaluation showed that the product that alcohol content and acidity were 1% and 0.8% respectively had the best palatability(p<0.01). 7. Chemical composition of final product was similar to that of milk koumiss in ash, protein and moisture content.

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The Effects of Mulberry Extract Consumption on the Serum Levels of Oxidant and Inflammatory Factors in Middle-aged Women with Rheumatoid Factors (오디추출물 투여가 류머티즘 요인이 있는 일부 한국 중년여성의 혈중 항산화 및 항염증 관련 지표수준에 미치는 영향)

  • Shin, Jung-Hee;Han, Se-Mi;Kim, Ae-Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.8
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    • pp.3561-3569
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    • 2012
  • This study investigated the effect of mulberry fruit extract(MFE) on antioxidant and anti-inflammatory activities of middle aged women with rheumatoid factor (RF). Thirty two middle-aged subjects were divided into two groups which were normal middle-aged group (NMG) and abnormal middle-aged group whose serum RF level were > 10 u/mL (AMG). All groups had consumed MFE (100 mL/day) for 4 weeks. Anthropometric measurements, serum inflammatory factors, serum oxidative stress markers analyses were performed at baseline and then at 4 weeks following the study. There were no significant differences in anthropometric measurements, including BMI, WHR and body fat composition between two groups. But after 4 weeks MFE consumption, serum levels of C-reactive protein (CRP), ferric-reducing ability of plasma (FRAP), serum TNF-${\alpha}$, IL-2, IL-4 had significantly decreased (p<0.05) in AMG. These findings suggested that the MFE consumption as food may be protective against oxidation and inflammation like RA.

Comparison of the SNR in the MR images on dental implant material (치아 임플란트 재료에 따른 자기공명영상의 SNR 비교)

  • Kim, Dong-Hyun;Ko, Seong-Jin;Ye, Soo-Young
    • Journal of the Institute of Convergence Signal Processing
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    • v.16 no.4
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    • pp.149-155
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    • 2015
  • Tooth implant is located in oral cavity and affects neck, skull base, and facail image. These magnetic inhomogeneities are usually frequency encoding direction which cause artifacts due to change of signal strength and geometric distortion. First, to evaluate signal to noise ratio (SNR) of magnetic resonance image caused by tooth implant this study uses meat phantom which is similar to human body and is consisted with fat, muscle, and water to measure signal to noise ratio. Second, signal to noise ratio by using custom-made fixed phantom is measured, and then signal to noise ratio size of different tooth implant types is compared and analyzed. The measured signal to noise ratio values of Brushite, HSA, Metal, and RBM for meat phantom were 2.76, 2.22, 1.88, and 1.57 on T1 SE, 1.88, 1.78, 1.65, and 1.79 on T2 FLAIR, 2.28, 2.25, 2.88, and 2.05 on T2 FSE, and 2.74, 1.94, 1.67, and 1.48 on T2 GRE. The measured signal to noise ratio values of Brushite, HSA, Metal, and RBM for fixed water phantom were 1.2, 1.06, 1.12, and 1.22 on DWI, 1.93, 1.87, 1.93, and 2.06 T1 SE, 1.83, 1.76, 1.82, and 1.92 on T2 FLAIR, 1.85, 1.79, 7.86, and 1.97 on T2 FSE, and 1.97, 1.93, 1.99, and 2.06 on T2 GRE. By considering through the results, patients and dentists need to consider some impacts from testing many aspects although their main purpose of having tooth implants is a dental restoration. Moreover, depending on the tooth implant characteristics of individual patients this study results can be used as baseline data when choosing test protocol.

A Basic Study on the Characteristics of the Modern Garden in Incheon During the Opening Period - Focused on Rikidake's Villa - (개항기 인천 근대정원의 조영특성에 관한 기초연구 - 리키다케 별장을 중심으로 -)

  • Jin, Hye-Young;Shin, Hyun-Sil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.3
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    • pp.83-91
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    • 2020
  • The purpose of this study is to examine the process of formation of modern gardens. Based on the analysis of the process of formation and transformation of the Jemulpo in Incheon and the details of the modern garden construction. The results are as follows; First, the formation of the Incheon Residence Site began in 1876 with the signing of the Joseon-Japan Treaty. Jemulpo used to be a desolate fishing village in the past, but after its opening in 1881, the Japanese settlement, Chinese settlement, and the general foreign settlement were formed. After that, Japan reclaimed the southern mudflats and expanded the theire settlement area, and advanced to the Joseon area(currently Sinheung-dong). In Japanese colonial era, modern Japanese urban landscapes were transplanted into the settlement area, centering on the Japanese modern gardens were distributed in the area around the center of the settlement area. Second, after examining the process of creating the garden for the Rikidake villa, Japanese Rikidake purchased a site for an orchard in Uri-tang, who was a major landowner in Incheon, to create the garden. At the time of Rikidake's residence, the garden was very large, measuring about 3,000 pyeong, and after liberation, it was acquired by Incheon City and used as Yulmok Children's Library. It was known as a rich village at the time of the opening of the port, and a garden was located at the highest point in Yulmok-dong, making it easy to see the Incheon Port area. Also, a spot located about 300 meters away from Rikidake's rice mill may have affected the location selection. Third, today's Rikidake villa has a Japanese-style house on a trapezoidal site, with a garden of about 990 square meters on the south side. Currently, it is possible to enter from the south and from Yulmok Children's Park in the north, but in the past, the main direction of the house was to view the Incheon Port, settlement area, and the Rikidake Rice Mill, so the house was located in front of the garden. The garden is a multi-faceted style with stone lanterns, tombstones, garden stones, and trees placed on each side, and is surrounded by arboreal plants such as attention, strobe pine, and maple trees, as well as royal azaleas. The view from the inside of the house was secured through shrub-oriented vegetation around the house.

Quality Investigation of Commercial Northern Sand Lance, Ammodytes Personatus Sauces (시판 까나리액젓의 품질조사)

  • CHO Young Je;IM Yeong Sun;LEE Keun Woo;KIM Geon Bae;CHOI Young Joon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.5
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    • pp.612-617
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    • 1999
  • The quality characteristics of 15 kinds of the commercial northern sand lance, Ammodytes Personatus sauce (CNS) of korean traditional salt-fermented fish sauces were evaluated comparing to the traditional northern sand lance, Ammodytes Personatus sauce (TNS). The ranges of chemical compositions of the CNSs were $66.5\~71.0\%$ moisture, $19.3\~24.6\%$ ash, $4.7\~12.0\%$ crude protein, and the pH and salinity were $5.56\~6.47\%,\;24.0\~32.9\%$, respectively. Total nitrogen, amino nitrogen, total free amino acid and total ATP related compounds (sum of ATP$\~$IMP, HxR, Hx and uric acid) were in the ranges of 0.781$\~$ 1,918 g, 445.9$\~$1037.9 mg, 3,258.9$\~$6:562.6mg in 100 ml CNSs, and 4.766$\~$8.989 $\mu$mol in 1 ml CNSs. The CNSs were higher in content of moisture, TMAO, TMA and pH, but lower in content of crude protein, salinity, total nitrogen, amino nitrogen, total ATP related compounds, absorbance at 453 nm and total free amino acid than the TNSs, Both CNS and TNS samples were rich in free amino acids, such as glutamic acid, lysine, alanine, leucine, valine, aspartic acid and isoleucine in the order.

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