• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.1
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• pp.24-30
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• 2010

Comprehensive studies on seedling production and transplanting cultivation of waxy corn are necessary to fulfill the comsumer's preference for the high quality whole ear waxy corn and its year-round supply for higher price, and to escape marketing in a short period at harvest season. Two waxy corn hybrids (cv. Chalok# 1 and Chalok# 4) were used to study the effects of seeding date, nursery days and plug cell size on growth and yield of waxy corn, and to clarify the reliable transplanting date at the Experimental Field of the National Institute of Crop Science in 2007. The number of days from seeding to silking was increased as nursery days were extended, but the number of days from transplanting to silking was shortened comparing to the direct seeding. Number of days from seeding to silking was shorter by 9~12 days for Chalok# 1 than for Chalok# 4, while the number of days from seeding to silking was shorter by 12~15 days in the second cropping than in the first cropping. Culm length of transplanted waxy corns was decreased as the nursery days were extended. Culm length of seeded Chaok# 1 and Chaok# 4 were decreased by 17% and 24%, respectively, in the second cropping compared to those in the first cropping. Ear length was somewhat decreased as the nursery days were extended. Chalok# 1 in the second cropping the degree of decrease was much higher in second cropping than in first cropping of Chalok# 1. Comparing to the first cropping, number of marketable ears per 10a of Chalok# 1 decreased as high as 64%, while that of seeded Chalok# 4 decreased mere 12% in the second cropping.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.265-265
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• 1994

본 연구는 간염 바이러스의 X 및 elongated X 유전자를 클로닝하여 E. coli에서 대량 발현시진 후, 그 기능을 여러 측면에서 연구하교 지금까지 알려진 oncogene products, tumor suppressor, 그리고 그 밖의 다른 암 유발인자와의 interaction에 대해 분석함으로써 간암 생성의 분자적 기작을 이해하고 더 나아가 간암의 예방 및 치료제의 개발을 목표로 하였다. 그 일차적 연구로서 이전에 플로닝된 mutant hepatitis Bvirus genome으로부터 X 및 elongated X 유전자를 클로닝하였으며, E. coli에서 대량 발현시키기 위하여 T7 bacteriophage promoter아래에 재 클로닝하였다. 이러한 X 및 ebngated X 유전자를 E. coli에서 대량 발현시킨 후, rabbit anti-X antibody를 이용하여 western blotting을 수행함으로서 이를 확인하였으며 DEAE-cellulose와 heparin-agarose chromategraphy를 이용하여 순수분리하였다. 순수분리된 X 및 etongated X 단백질을 highly differentiated hepatoma cell인 HepG$_2$ cell에 처리하여 transactivation activity를 측정하였다. 그 결과 순수분리된 단백질들이 SV4O promoter를 transactivation 함을 할 수 있었으며, 이로부터 클로닝된 유전자들이 모두 정상적인 기능을 가짐을 확인하였다. 그러고 X 유전자의 작용기작을 규명하기위하여 restriction endonuclease를 이용하여 5 개의 mutant X 유전자를 구성하였으며 현재 이를 HepG2 cell에 transfection 하여 그 기능을 연구하고 있다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.167-167
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• 1993

신물질의 면역독성 평가방법을 체계화하고 독성기작을 규명하고저 기존 면역독성 유발물질을 대상으로 in vivo 및 in vitro 실험을 수행하였다. 먼저 비장세포-간세포 공동배양 시스템을 대사를 요구하는 물질의 면역독성 평가에 in vitro system으로 사용하고저 DMN을 대상으로 공동배양 조건 및 배지에 대한 연구를 수행하였다. 비장세포원으로 BALB/c 마우스를 사용하였고, 간세포원으로 Spraque-Dawley 랫드를 사용하였다. DMN은 홀몬이 첨가된 배지에서 항체 형성반응을 크게 저하시켰으나 배지의 종류에 따른 DMW의 대사에는 큰 영향이 없었다. 또한 procarcinogen인 DMBA에 의한 면역독성은 DMBA의 대사물에 의해 유도되며 DNA가 primary target 임을 규명하였으며, 2-acetylaminofluorene (AAE)에 의한 면역 억제작용의 기작에서 인터루킨의 영향에 대해 연구하였다. Protein methylase inhibitor인 SF와 SIBA웨 면역억제 작용을 연구한 결과 lymphoproliferative response에서 protein methylase 가 직접 또는 간접적으로 관여함이 입증되었다.

• The Korean Journal of Zoology
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• v.25 no.3
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• pp.107-114
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• 1982

A combination of ultraviolet irradiation and egg rotation was applied to Korean frogs to study the mechanisms of the establishment of the dorsal-ventral polarity. Rotation of the uncleaved egg was capable of preventing the characteristic syndromes associated with UV irradiation. As well, brief rotation of the egg before cleavage relocates the site of dorsal lip to a novel location in the embryo at gastrula stage. That is, the dorsal lip appeared on the opposite side to gravity at the rotation of the egg. Above results were discussed with the informations obtained from other anuran and urodele species, and were interpreted in terms of regulating mechanisms for early embryogenesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.14
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• pp.53-64
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• 1973

The studies reported herein were conducted to investigate the effect of thermal conditions in double-cropping of rice. The accumulated daily mean and minimum air temperatures, for the period of the last 30 years, were examined at the 10 different meteorogical stations which are located in the southern part of Korea. The results obtained could be summarized as follows: 1. The first cropping. a. It seemed to be free from any frost-damage of rice at the seeding stage at Yeosu, Pusan and Cheju. However, it was found that there were some dangers of frost damage for about 30 to 40 day at Iri, Chonju and Kwangju, for 18 to 28 days at Daeku and Ulsan, and for 4 to 14 days at Mokpo and Pohang, respectively. b. The early critical transplanting date seemed to be from middle to late-April in the first cropping. As compared with the ordinary lowland seedlings, the semi-protected and upland ones could be planted 5 and 10 days earlier, respectively. c. The early critical heading date was about late-June and there were some low-temperature damages for 8 to 25 days at young-ear formation stage of rice plant, depending upon location. d. The early critical ripening date (the early critical transplanting date of the 2nd cropping) was from late-July to early-August. It took about 32 to 39 days in ripening. There was a tendency of SS${\fallingdotseq}$SL$15^{\circ}C$ (${\theta}$15) and the minimum of $10^{\circ}C$ (${\theta}$10), the ten locations could be devided into two ripening groups of ${\theta}$15>${\theta}$10 and ${\theta}$15<${\theta}$10. c. The late critical ripening date was around October 9 at Iri, Chonju, Kwangju and Daeku and around October 28 at Mokpo, Yeosu, Pusan and eheju. Three to four days were more required for a complete ripening of rice, as compared with the above dates. d. There was an overlap of about 12 to 42 days between the first and second cropping when early-maturing varieties requiring an accumulated mean air temperature of $1, 550^{\circ}C$, from transplanting to heading, were grown. Therefore, some varieties which could head with an accumulated daily mean air temperature of 1, 000 to $1, 200^{\circ}C$, should be either developed or some new cultural technology be established in order to have a successful double cropping in rice.

• Journal of Life Science
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• v.23 no.8
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• pp.963-969
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• 2013

Although it is popularly believed that vitamin C protects cells from various genotoxicants, the degrees and mechanisms of itsprotective actions are not fully understood. In this study, vitamin C's protective effects against various genotoxicants were quantified, together with subsequent analyses on the mechanisms of these protective effects. Comet assay was employed to measure the degree of DNA damage in Chinese hamster ovary cells (CHO-K1) exposed to five genotoxicants, $H_2O_2$, $HgCl_2$, N-methyl-N-nitro-N-nitrosoguanidine (MNNG), 4-nitroquinoline-1-oxide (4NQO), and UV-irradiation. In cases cells were treated with $H_2O_2$, $HgCl_2$, and 4NQO together with vitamin C, the damage to DNA decreased to the level of the control group. In cases of UV-irradiation, the protective effect of vitamin C appeared, but did not reach the control levels. Interestingly, vitamin C did not have protective effects against the genotoxicity of MNNG. The degrees of DNA damage of cells treated with vitamin C prior to exposure togenotoxicants were 28~49% lower than those of cells treated with vitamin C after being exposed to genotoxicants. In conclusion, vitamin C had strong antioxidanteffects against genotoxicants by being a primary antioxidant blocking genotoxicity reaching the cells, rather than being a secondary antioxidant acting on post-exposure DNA repair processes. However, vitamin C's protective effects appearto be limited, as there are genotoxicants, such as MNNG, whosegenotoxicityis not affected by vitamin C. Therefore, the results of this study warrant furtherstudies on toxic mechanisms of genotoxicants and their interactions with protective mechanisms of vitamin C.

• Journal of Wetlands Research
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• v.20 no.1
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• pp.63-71
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• 2018

The particle filtration mechanisms in an infiltration trench should be varying due to the different hydraulic conditions during stormwater runoff. The understanding of these variations associated with different filtration mechanisms and their effect on the particle removal efficiency is of vital importance. Therefore, a LID (Low Impact Development) system comprising of an infiltration trench packed with gravel and woodchip was investigated during the monitoring of several independent rainfall events. A typical rainfall event was divided into separate regimes and their corresponding flow conditions as well as filtration mechanisms in the trench were analyzed. According to hydraulic conditions, it was found out that filtration changes between vertical and horizontal flows as well as between unsaturated, saturated, and partially-saturated flows. Particle separation efficiency was high (55-76%) and was mainly governed by physical straining during the unsaturated period. It was then enhanced by diffusion during the saturated period (75-95%). When the trench became partially saturated at the end of the rainfall event, the efficiency decreased which was believed to be due to the existence of a negatively charged air-water interface which limited the removal to positively charged particles.

• Journal of Life Science
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• v.31 no.8
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• pp.771-777
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• 2021

Human endogenous retroviruses (HERVs) were inserted into the human genome millions of years ago but they are currently inactive and non-infectious due to recombinations, deletions, and mutations after insertion into the host genome. Nonetheless, recent studies have shown that HERV-derived elements are actually involved in physiological phenomena and certain diseases including cancers. Among the various physiological phenomena related to HERV-derived elements, it is necessary to focus on inflammatory response. HERV-derived elements have been reported to be directly involved in various inflammatory diseases, including autoimmune diseases such as rheumatoid arthritis, multiple sclerosis, amyotrophic lateral sclerosis, and Sjogren's syndrome. As a mechanism for regulating inflammation through HERV-derived elements, the possibility that HERV-derived elements may cause nonspecific innate immune processes and that HERV-derived RNA or proteins may cause selective signaling mechanisms through specific receptors can be considered. However, the mechanism through which HERV-derived elements regulate inflammatory response, such as how silent HERV elements are activated in inflammatory response and what factors and signaling mechanisms are involved in HERV-derived elements, have not been identified to date, making it difficult to study the onset of HERV-related inflammatory disease. In this review, we introduce HERV-related autoimmune diseases and propose the mechanisms of HERV-derived elements at the molecular level of HERV in inflammatory response.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.271-271
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• 1994

NADPH-dependent succinic semialdehyde reductase를 소의 뇌조직으로부터 여러가지 크로마토그래픽 방법을 이용하여 순수 분리 정제하였다. 효소는 분자량 34kDa을 가진 monomeric 단백질이며 substrate specificity. 분자량, 최적 pH, 아미노산 조성 등을 다른 sources의 효소들과 비교하였다. 이 비교 결과들로부터 본 연구에서 정제한 효소는 다른 sources와 다른 효소로 밝혀졌다. 반응의 산물이나 유사 기질 등을 저해제로 사용하였을때의 반응기작은 intermediate ternary complex를 형성하고 NADPH가 먼저 효소에 binding하는 ordered Sequencial mechanism을 보인다는 사실을 관찰할 수 있었다.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 1999.10a
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• pp.135-136
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• 1999

대기 침적은 대기중으로 방출된 오염물질의 제거기작으로 널리 알려져 있으며, 대기로부터 자연계의 표면에 침적되는 대기중의 유해물질들이 환경계에 미치는 영향과 그들의 이동을 조절하는 중요한 기작의 하나이다. 그러나 건식 침적의 경우 공간 및 시간적 변화가 크고 침적되는 표면과 대기 사이의 상호 반응이 침적되는 물질의 양에 커다란 영향을 미칠수 있기 때문에 그 측정이 상당히 어렵고 또한 시료 채취시 세심한 주의와 축적된 경험을 요구한다.(중략)