• Proceedings of the KIEE Conference
• /
• 2011.07a
• /
• pp.1690-1691
• /
• 2011

본 논문에서는 H1N1 인플루엔자 A 바이러스(influenza A H1N1 virus) 검출을 위한 산화아연 나노구조(zinc oxide nano structure) 기반의 전기화학적 면역센서를 제작하고 그 특성을 분석하였다. H1N1 인플루엔자 A 바이러스는 빠른 전파 속도 때문에 정확하고 빠른 검출이 필요하다. 먼저, 2 $mm^2$의 표면적을 갖는 패턴된 금 전극 위에 열수방식(hydrothermal method)으로 성장시킨 산화아연 나노구조가 선택적으로 형성되도록 리프트-오프(lift-off) 방법을 사용하였다. 0.01 M phosphate buffered saline(pH 7.4)에서 2 ${\mu}g$/mL 농도의 1차 항체를 정전기력에 의해 산화아연 나노구조에 고정화한 후, 10 pg/mL ~ 5ng/mL 농도의 H1N1 항원을 적용하여 포획 항체에 결합시키고 HRP(horseradish peroxidase) 효소가 결합된 검출 항체를 항원에 결합시키는 샌드위치 ELISA법을 이용하였다. HRP와 반응하는 TMB(3,3', 5,5'-tetramethylbenzidine)와 과산화수소가 포함된 acetate buffered 용액(pH 5)을 전해질로 사용하고 순환전압전류 측정법(cyclic voltammetry)으로 센서의 특성을 분석하였다. 측정된 순환전압전류그래프(cyclic voltammogram)에서 H1N1 항원 농도 10 pg/mL ~ 5 ng/mL의 응답 전류는 276.47 ${\pm}$ 21.72 nA (평균 ${\pm}$ 표준편차, n=4) ~ 478.89 ${\pm}$ 6.21 nA로 측정되었고, logarithmic하게 증가하는 응답 전류 특성을 보였다.

• Resources Recycling
• /
• v.23 no.6
• /
• pp.30-39
• /
• 2014

The trajectories of PCB(Printed Circuit Board) particles in the corona discharge electrostatic separation was simulated. The PCB particles are prepared by crushing bare board, which disassembled from electronic components, consist mostly of copper and FR-4(Flame Retardant Level-4) Firstly, a model was established for calculating of detachment points of PCB particles from the rotating electrode in separator. The model of detachment points was derived from equilibrium of force such as gravity force, centrifugal force, electrostatic force. The trajectories of particles after detachment was calculated by acceleration derived from time-integrating method of motion equation. In this simulation, particle size, supplied voltage, rotation speed of rotating roll electrode and angle of induction electrode were adopted as variables. While the trajectories of FR-4 particles were affected by all variables, rotation speed of rotating roll electrode was dominant variables affecting trajectories of copper particles.

• Journal of the Korean Society for Precision Engineering
• /
• v.31 no.1
• /
• pp.83-90
• /
• 2014

EHD (electro-hydro-dynamics) patterning was performed under atmospheric pressure at room temperature in a single step. The drop diameter smaller than nozzle diameter and applied high viscosity conductive ink in EHD patterning method provide a clear advantage over the piezo and thermal inkjet printing techniques. The micro electrode pattern was printed by continuous EHD patterning method using 3-type control parameters (input voltage, patterning speed, nozzle pressure). High viscosity (1000cps) conductive ink with 75wt% of silver nanoparticles was used. EHD cone type nozzle having an internal diameter of $50{\mu}m$ was used for experimentation. EHD jetting mode by input voltage and applied 1st order linear regression in stable jet mode was analyzed. The stable jet was achieved at the amplitude of 1.4~1.8 kV. $10{\mu}m$ micro electrode pattern was created at optimized parameters (input voltage 1.6kV, patterning speed 25mm/sec and nozzle pressure -2.3kPa).

• Journal of the Korean Institute of Gas
• /
• v.21 no.5
• /
• pp.9-15
• /
• 2017

Natural gas fuel has known to be very promising in terms of abundancy and economic value. Therefore it is widely treated as research topics in a variety field of production, storage and utilization. Natural gas has become one of the major sources for the power generation by using internal combustion engines(ICE). Development of natural gas fuel injection device should be preceded to realize a reliable natural gas fuel supply system for a MW class power generation reciprocating ICE. In this research, an injection valve which consists of solenoid and body part with a moving plate was designed and its dynamic performance was experimented in the engine-like environment. Displacement length and diameter of an armature and diameter of a solenoid coil were tested at former study. In this research the effect of materials of solenoid core, size of main housing inlet and supply gas pressure are examined.

• 한국신재생에너지학회:학술대회논문집
• /
• 2011.05a
• /
• pp.113.1-113.1
• /
• 2011

PEMS (printed electro-mechanical system) is fabricated by means of various printing technologies. Passive and active compo-nents in 2D or 3D such as conducting lines, resistors, capacitors, inductors and TFT(Thin Film Transistor), which are printed withfunctional materials, can be classified in this category. And the issue of PEMS is applied to a R2R process in the manu-facturing process. In many electro-devices, the vacuum process is used as the manufacturing process. However, the vacuum process has a problem, it is difficult to apply to a continuous process such as a R2R(roll to roll) printing process. In this paper, we propose an ESD (electro static deposition) printing process has been used to apply an organic solar cell of thin film forming. ESD is a method of liquid atomization by electrical forces, an electrostatic atomizer sprays micro-drops from the solution injected into the capillary with electrostatic force generated by electric potential of about several tens kV. ESD method is usable in the thin film coating process of organic materials and continuous process as a R2R manufacturing process. Therefore, we experiment the thin films forming of PEDOT:PSS layer and active layer which consist of the P3HT:PCBM. The organic solar cell based on a P3HT/PCBM active layer and a PEDOT:PSS electron blocking layer prepared from ESD method shows solar-to-electrical conversion efficiency of 1.42% at AM 1.5G 1sun light illumination, while 1.86% efficiency is observed when the ESD deposition of P3HT/PCBM is performed on a spin-coated PEDOT:PSS layer.

• Microbiology and Biotechnology Letters
• /
• v.36 no.1
• /
• pp.82-85
• /
• 2008

Hyungbangjihwang-Tang (HT), an Oriental herbal formula, has been known to play a role which helps to recover vigor of human in the Orient. In this study, antibacterial substance (HTI) was purified from the ethyl-acetate extracts of HT by using $SiO_2$ column chromatography and HPLC, and the antibacterial effects of HTI were investigated. By using the CLSI broth micro-dilution assay, the activity of HTI was evaluated against human pathogenic Gram-positive and Gram-negative bacterial strains including the clinical isolates of methicillin-resistant Staphylococcus aureus. The results demonstrated that HTI showed broad spectrum antibacterial activities against all bacterial strains tested. In conclusion, HTI is an interesting new molecule for its potential in anti-infective drug discovery and for future studies on activity-structure relationship through analysis of its chemical structure.

• Microbiology and Biotechnology Letters
• /
• v.37 no.3
• /
• pp.273-279
• /
• 2009

Hyungbangjihwang-Tang (HT), an Oriental herbal formula, has been known to play a role which helps to recover vigor of human in the Orient. In this study, antifungal substance (HTI) was purified from the ethyl-acetate extracts of HT by using $SiO_2$ column chromatography and HPLC, and the antifungal effects of HTI and its mode of action were investigated. By using a broth micro-dilution assay, the activity of HTI was evaluated against fungi. HTI showed antifungal activities without hemolytic effect against human erythrocytes. To confirm antifungal activity of HTI, we examined the accumulation of intracellular trehalose as stress response on toxic agents and effect on dimorphic transition in Candida albicans. The results demonstrated that HTI induced the accumulation of intracellular trehalose and exerted its antifungal effect by disrupting the mycelial forms. To understand its antifungal mode of action, cell cycle analysis was performed with C. albicans, and the results showed HTI arrested the cell cycle at the S phase in yeast. The present study indicates that HTI has considerable antifungal activity, deserving further investigation for clinical applications.

• Journal of KIISE:Software and Applications
• /
• v.32 no.8
• /
• pp.769-780
• /
• 2005

This paper addresses a novel application of meshes to analyzing the deformation patterns of 2D signals. The proposed mesh is distinguished form the previous models in that it includes simulated charges in each node that interact with external charges comprising an input pattern. Therelaxation of the mesh given an input is carried out by any of the well-known numerical integration techniques. The result of the relaxation is a deformed mesh. This Paper provides four criterion functions for measuring the pattern deformation. A set of trained meshes was created from the simple average of target patterns. Experimental results show that these measures, although highly intuitive, are not good enough to capture the amount and characteristics of pattern deformation. If more sophisticated measures are found and incorporated into the relaxation process, we expect that a better and high-performance mesh framework is realized.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.27 no.4
• /
• pp.191-195
• /
• 2017

The microstructures of nonwoven fabric with and without nanofibers used as a filter for air purification type mask were investigated using scanning electron microscope. Moreover, we proposed a new mask design which is effective in the improvement of the fine dust blocking property. When comparing to nonwoven fabrics of which an average diameter was $25{\mu}m$, the nanofibers formed by the electrospinning process had a tight mesh structure arranged irregularly with a relatively large specific surface area, which could be associated with their much smaller diameter ranging from 25 to 120 nm. Such a prominent structural feature at nanofibers led to mechanical adsorption of fine particles, resulting in enhancement of filtering behavior maintaining high permeability. In addition to the excellent performance of the mask filter, wearing the mask properly is expected to maximize the blocking property of fine dust. To meet such a requirement, a new mask design that can be closely attached to the face in order to effectively block fine dust entering the gap between the face and the mask.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.15 no.6
• /
• pp.122-128
• /
• 2016

The Biosensor biosensor pattern was developed by via an EHD (electro-hydro-dynamics (EHD) patterning process that was performed under atmospheric pressure at room temperature in a single step. The drop diameter was smaller than nozzle diameter and applied high viscosity conductive ink was applied in the EHD patterning method to provide a clear advantage over the piezo and thermal inkjet printing techniques. The Biosensor's biosensor's micro electrode pattern was printed by via a continuous EHD patterning method using 3three- type types of control parameters parameter (input voltage, patterning speed, nozzle pressure). High viscosity (1000 cps) conductive ink with 75 wt% of silver nanoparticles was used for experimentation. The incremental result of impedance of biosensor impedance was measured between the antibody ($10ug{\mu}g/ml$) to spore (0.1 ng/ml, 10 ng/ml, and $1ug{\mu}g./ml$) reaction at frequency 493 MHz frequency.