• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.37-37
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• 2020

홍띠(Imperata cylindrica 'Rubra') 식물자원의 생장점 부위를 기내 배양하여 기내 식물체 재분화와 재분화식물체의 유전적 안정성을 검토하였다. 기내배양은 26±2 ℃, 25 μmol/m²/s, 14h/10h (day/night) 광조건 하의 배양실에서, MS (Murashige and Skoog, 1962) 기본배지에 생장조절물질을 첨가하여 조직절편체로부터 식물체를 유도하였다. 캘러스는 MS기본배지에 0.1 mg/L의 2,4-D와 2 mg/L의 BA를 혼용처리하여 생장점 부위로부터 유도하였다. 캘러스 증식은 MS기본배지에 0.1 mg/L의 2,4-D를 첨가한 배지에서, 이들 캘러스로부터 신초 재분화는 0.01 mg/L의 NAA 및 2 mg/L의 BA를 첨가한 배지에서 유도하였다. 다경줄기 형성(multiple shooting) 후 MS배지에서 4주 동안 배양한 재분화식물체는 멸균한 상토(버미큘라이트)를 포함한 배양병에서 7주간 배양한 다음 점차적으로 배양병 뚜껑을 개방(1/10 정도 1차 개방 1주일, 3/10 정도 2차 개방 2주일)하여 직경 6 cm의 컵포트에 이식하여 활착시켰다. 재분화식물체는 붉은색이 사라지고 녹색을 나타내었으며, 일부개체에서만 잎의 일부분만 붉은색을 나타냈다. 이는 생장점 주변조직에서 유래한 재분화체가 우세함으로써 생장점보다는 생장점 주변의 조직을 구성하는 녹색층에서 주로 식물체가 재생되는 것으로 판단된다. 이에 따라 홍띠 대조구식물체 8개체, 활착한 녹색 재분화 식물체 20개체(실내 재배중인 순화체 10개체, 2020년 6개월간 포장에서 재배중인 순화체 10개체)를 대상으로 ISSR분석을 실시하여 재분화식물체의 유전적 안정성을 검토하였다. 향후 조직학적 측면에서 신초재분화의 기원에 대한 검토가 필요하다고 사료된다.

• Journal of Life Science
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• v.12 no.6
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• pp.740-744
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• 2002

This experiment was conducted to clarify the effects of physiological properties in culture media on the proliferation and the acclimation of bulblets of several kinds of lilies cultured in vitro. Proliferation rate of bulblets from bulblet of Oriental hybrid ‘Marco Polo’, ‘Casa Blanca’ and Asiatic hybrid ‘Jolanda’ was higher in solid MS media compared with bulblets from scale Acclimation rate of regenerated bulblets was high in solid media which was pre-cultured in vitro, compared with liquid media. Sprouting rate of bulblets from bulblet was higher than bulblets from scale.

• Korean Journal of Plant Tissue Culture
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• v.21 no.4
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• pp.221-225
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• 1994

This experiment was conducted to identify the optimal in vitro propagation condition of Cnidii rhizoma (Cnidium officinale Makino). It was effective to reduce contamination and improve regeneration of shoot when shoot tips taken in July were cultured in 1/2 strength Murashige and Skoog medium supplemented with 500 mg/L carbenicillin disodium 1.0 mg/L BA and 1.0mg/L $GA_3$followed by surface sterilization of explant source in solution of 1% sodium hypochlorite for 20 minutes. When shoot tips were 쳐cultured in 1/2 strength MS medium with 0.5 mg/L BA and 60 g/L sucrose, shoot elogation and subsequent multiplication of the formed shoot were favorable than in other media. Regenerants were well rooted in 1/2 strength MS medium containing 3.0 mg/L NAA.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.53-57
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• 1995

This study was carried out to investigate the effect of TDZ (thidiazuron) treatment on Propagation in vivo through scale cultures of Narcissus pseudonarcissus. Scales with disk (5 to 7 m in size) were cultured on MS medium containing NAA, BA and TDZ. Bulbs and shoots were directly formed when scales were cultured on medium containing 5 mg/L NAA and 1 mg/L BA. In addition, combination of 3 mg/L NAA and 0.02 mg/L TDZ promoted effectively the direct formation of bulbs and shoots. The shoots were rooted when cultured on medium containing 5 mg/L NAA and 0.02 mg/L TDZ. non scales obtained from regenerates were cultured on medium containing 1.0 mg/L NAA and 0.5 mg/L TDZ, they gave rise to numerous bulbs and shoots . The overall results suggest that TDZ is an effective plant growth regulator in vitro propagation of N. pseudonarcissus.

• Korean Journal of Plant Tissue Culture
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• v.28 no.4
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• pp.227-230
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• 2001

Kiwi (Actinidia deliciosa) is exotic plant and thus susceptible to cold climate in the middle part of Korean peninsular. Several hybrids have recently been developed to enhance cold tolerance by crossing them with domestic species (A. arguta), We have developed an efficient micropropagation technique for the hybrids using both axillary bud and callus culture systems. Shoot proliferation from axillary buds was possible on St medium supplemented with 0.2 mg/L Bh and 3.0 mg/L GA$_3$. In vivo cuttings of the proliferated shoots were more effective for root induction and subsequent survival than in vitro rooting. More than 95% of the plantlets were successfully transferred to field. Effective callus induction was achieved on MS or B$_{5}$ medium with 2,4-D or NAA. Although callus induction could be made from any combinations of media and auxins, shoot regeneration was observed only from the callus induced on medium containing NAA.A.

• KSBB Journal
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• v.17 no.6
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• pp.582-585
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• 2002

In an effort to use plant biotechnology for the production of biopharmaceuticals, pollens collected from lily (Lilium longiflorum) were grown in vitro and transformed with a PCR-amplified 1.7 kb cDNA encoding human tissue plasminogen activator (tPA) using Agrobacterium via a vacuum infiltration process. Western blotting showed that transgenic lily pollen tubes selected on kanamycin for 16 hrs expressed a tPA protein with a size similar to the human standard, suggesting their possible use as a disposable host for rapid foreign protein production.

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.101-104
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• 1995

The experiment was conducted to identify the optimal in vitro propagation condition for P. lactiflora Pall. Through apical shoot tip and axillary shoot tip culture of winter bud. When apical shoot tip and axillary shoot tips excised from winter bud were cultured on MS medium supplemented with various concentrations of plant growth regulators, all the apical shoot tips elongated regardless of the composition of the medium but axillary shoot tips responded differently. Shoot of 'Uisong' local cultivate was well elongated in the medium containing 0.01mg/L NAA. Frequency of shoot formation and subsequent shoot growth in axillary shoot tip culture were promoted in the medium containing 0.01 mg/L NAA and 5.0mg/L zeatin. 30% of the elongated shoots were vigorously rooted on the medium containing 0.1mg/L NAA with vermiculite as a support medium.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.271-274
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• 1999

This study was undertaken to develop an efficient propagation technique for mature Betula davurica. Using aseptic materials taken from in vitro culture, the effects of media and plant growth regulators on shoot proliferation and rooting were investigated. DKW medium turned out to be the best in shoot proliferation among the media tested. Whereas axillary buds were better culture material than apical buds in proliferation of shoots, apical buds were slightly better than axillary buds on shoot elongation. Neither 1 /2 MS nor WPM medium seemed to be suitable for shoot multiplication or elongation. When the explants were cultured on 1/2 MS medium, shoot elongation was retarded by forming big callus at the base. In the case of WPM, shoots could be formed normally, but they exhibited slow growing. NAA was so effective on in vitro rooting that more than 80% rooting could be achieved on half-strength DKW medium supplemented with 1.0 mg/L NAA after 4 weeks in cultures. Ex vitro rooting using elongated shoot was also applicable to rooting and acclimatization. Rooted plantlets were successfully acclimatized in an artificial soil mixture and grew normally. The results demonstrate that efficient mass propagation of mature B. davurica can be done through tissue culture.

• Korean Journal of Medicinal Crop Science
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• v.13 no.4
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• pp.154-160
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• 2005

This study was performed to compare anticancer and immune activities between natural Artemisia capillaris Thunb. extract and tissue cultured plant extract (hairy root, in vitro culture, callus). The inhibitory effect of cancer cell growth, human B cell growth and productivity of cytokines were examined. Furthermore, HPLC analysis was performed to confirm the components. The anticancer activities increased by more than 55% with the cultured callus of Artemisia capillaris T. for four cancer cell lines(Lung carcunoma, Stomach adenocarcinoma, Hepatocillular carcinoma, Breast adenocarcinoma), showing higher effect than natural Artemisia capillaris T. The extracts from hairy root and in vitro culture of Artemisia capillaris T. significantly increased the immune B cell growth. The immune B cell growth effect of natural Artemisia capillaris T. was higher than that of the tissue culture plants such as hairy root, in vitro culture and callus. Both natural and tissue cultured plants showed similar effects on cytokine secretion. The similar peak size was observed between natural Artemisia capillaris T. and cultured callus in HPLC analysis. As a results, the biological activities were not observed the difference between natural Artemisia capillaris T. and cultured callus. Thus, the cultured callus will be altered natural Artemisia capillaris T. in the environmental side and the resources preservative side

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.99-102
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• 1998

The most effective medium for shoot initiation in vitro of peach cv. Baekmijosaeng, Yumyeong and nectarine cv. Cheonhong was Quoirin and Lepoivre medium(LP). 20 g/L and 30 g/L sorbitol as carbon source were effective for shoot proliferation of cv. Baekmijosaeng. Addition of 500 mg/L lacto albumin enzymatic hydrolysate(LH) increased shoot number per explant of cv. Baekmijosaeng peach. Removing the apical meristem and horizontal placing of explants on the medium increased cv. Baekmijosaeng shoot.