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Analysis of Tissue Plasminogen Activator Expression using Pollen Culture in vitro  

박인혜 (대구가톨릭대학교 생명자원학부)
박희성 (대구가톨릭대학교 생명자원학부)
Publication Information
KSBB Journal / v.17, no.6, 2002 , pp. 582-585 More about this Journal
Abstract
In an effort to use plant biotechnology for the production of biopharmaceuticals, pollens collected from lily (Lilium longiflorum) were grown in vitro and transformed with a PCR-amplified 1.7 kb cDNA encoding human tissue plasminogen activator (tPA) using Agrobacterium via a vacuum infiltration process. Western blotting showed that transgenic lily pollen tubes selected on kanamycin for 16 hrs expressed a tPA protein with a size similar to the human standard, suggesting their possible use as a disposable host for rapid foreign protein production.
Keywords
Lily pollen; tansformation; tissue plasminogen activator; plant biotechnology;
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