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어류 초기 자어의 로티퍼 최초 섭이 일령과 포식수 분석을 위한 새로운 관찰 및 계수법

  • 정민민;민광식;지영주;위종환
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.22-22
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    • 2003
  • 로티퍼는 해산어와 담수어뿐만 아니라 식용어와 관상어에 이르기까지 모든 어류의 인공 종묘 생산 과정에서 초기 먹이생물로 이용되고 있는 중요한 동물 플랑크톤이다. 그리고 로티퍼를 초기 자어의 첫 먹이로 사용하는 것을 시작으로 알테미아, 코페포다 또는 초미립자 사료 등을 사용하는 어류 인공종묘 생산 과정에서의 먹이계열이 확립되어 있다. 그러나, 사육하고자 하는 어종에 따라 초기 자어기에서 로티퍼를 섭이하는 시기와 사육 일령별 자어의 로티퍼 포식수를 알 수 있는 정확한 방법이 없다는 이유로 지금까지는 자어의 로티퍼 최초 포식 일령 및 로티퍼 포식수를 개개의 연구자들 나름대로의 방법으로 관찰하여 왔다. 일반적으로 자어가 로티퍼를 포식하였는지의 여부 및 포식수는 자어 소화기내에 존재하는 소화가 진행되기 전의 로티퍼 몸체의 수를 계수하는 방법으로 이루어졌다. 로티퍼의 체내에는 섬모환으로 모은 수중의 먹이 현탁액을 소화기계로 이동시키기 전에 먹이를 잘게 다지고 부수는 기능을 하는 저작기 (trophi)가 존재한다. 그리고 이 저작기는 로티퍼의 종에 따라서 다른 형태를 하고 있는 것으로 알려져 있다. 이 연구에서는 자어가 섭이한 로티퍼는 어류의 소화기내에서 소화가 진행되는 과정에서 로티퍼의 체 구조 중 저작기가 쉽게 소화되기 어렵고 소화기관의 연동 운동에 의해서도 쉽게 그 모양이 손상되지 않는다는 것을 발견하고 이 저작기의 수를 계수하므로서 로티퍼의 최초 섭이 일령 및 일령별 로티퍼 포식수를 계수할 수 있는 방법을 검토하였다. 그 결과, 소화기내의 잔존 로티퍼 몸체만으로 계수 관찰하던 기존 방법에 비교하여 로티퍼의 저작기를 계수하는 방법을 이용할 경우 보다 신뢰도가 높은 결과를 도출할 수 있음을 확인하였다. 경제적 가치의 우수성 때문만은 아니다. 미국은 자국의 대두 생산자들이 생산해 낸 대두를 판매하면서 조성한 자조금을 모아 대두의 생산성을 향상시키기 위한 연구 개발과 국내외 시장을 확대하기 위한 마케팅 활동에 쓰고 있다. 이런 자조 노력은 비단 미국의 대두 농민들에게만 국한된 것이 아니다. 가깝게는 일본의 수산업과 해산어 양식산업의 발전은 중앙 및 지방 정부, 수협과 같은 어민 조합 및 연근해 수산업 종사자들이 출연한 기금을 통해 이루어지고 있음을 지난 8월 북해도의 양식산업을 시찰했을 때 확인할 수 있었던 사실이다. 이제 우리나라도 국민소득 1만불 시대를 넘어 2만불 시대를 열기 위한 청사진을 그릴 만큼 모든 경제의 규모가 확대되었을 뿐 아니라 질적으로 고도화되었다. 우리에게 필요한 것은 성숙된 산업사회에 걸맞는 각 산업 주체들의 의식이다. 한국의 양식산업도 자조 정신을 갖추어 생산성 향상에 초점을 맞춘 양식기술의 개발, 환경 보전을 고려한 양식장 관리 및 소비를 확대하기 위한 유통시스템의 선진화에 스스로 투자를 해야 할 시기에 와 있다.치가 완전히 익은 15일 후에는 무첨가 김치, 0.1%, 0.2% 및 0.3% 고분자 키토산 김치의 젖산균 수는 5.4X107 cfu/g, 3.3X107 cfu/g, 8.6X106 cfu/g 및 2.6X106 cfu/g로 나타났다. 5. 발효가 진행 중에 대장균 군 수가 시간과 더불어 대장균군 수가 모든 구에 있어서 6일까지는 서서히 증가하다가 그 후부터는 대장균 군 수가 감소하였다. 즉, 발효초기에는 모든 구가 2.0X104∼4.0X105 cfu/g이었고, 6일 경에는 8.9X104∼4.5X105 cfu/g로 약간 증가하였으며 15일후에는 키토산 무첨가 김치가 2.0X

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The Implementation Status of Dental Treatment Infection Control Standards of Dental Hygienists (치과위생사의 치과진료 감염방지기준에 관한 연구)

  • Yoo, Ha-Na;Kang, Kyung-Hee
    • Journal of Digital Convergence
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    • v.11 no.12
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    • pp.649-656
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    • 2013
  • This study was conducted to find out about the implementation status of dental treatment infection control standards of dental hygienists. The subjects of research were the dental hygienists working at dentist offices in Chungnam, Cheonan-si using self-input method from July 25th, 2012 to August 1st 2012. The collected data was analyzed after computerized statistical processing using SPSS 18.0. For hepatitis B vaccination, the results were high with 75.0% of answers being 'yes' or auto-active immunity, but for the latest hepatitis vaccination period showed highest results in '5 years or more ago' with 48.0%. Although 93.0% answered that vaccination was important, the percentage of replies that they had vaccination education was relatively low with 41.0%. For the use of personal protection tools the use of protective goggles was low compared to the use of masks and medical gloves. The percentage of subjects that answered that they always wash their hands before treatment was relatively low with 56.0% compared to 82.0% of subjects that answered that they always washed their hands after treatment. Dental treatment equipment washing before sterilization, use of packing and re-sterilization of tools with damaged packing showed high results for 'yes', but the ratio of subjects that answered 'no' to water line management was low with 39%.

DNA barcoding of Raptor carcass collected in the Paju city, Korea (파주시에서 수집한 폐사체 맹금류의 DNA 바코드 연구)

  • Jin, Seon-Deok;Paik, In-Hwan;Lee, Soo-Young;Han, Gap-Soo;Yu, Jae-Pyoung;Paek, Woon-Kee
    • Korean Journal of Environment and Ecology
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    • v.28 no.5
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    • pp.523-530
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    • 2014
  • One juvenile raptor which was not able to be identified due to its head damage was discovered on a roadside in Janggok-ri, Jori-eup, Paju on 28th June, 2011. The species was identified by DNA barcoding. After polymerase chain reaction (PCR) of the mitochondrial cytochrome c oxidase subunit I gene (COI), we obtained 695 bp sequences. We analyzed the obtained COI sequence with similar sequences from the BOLD systems and BLAST of the NCBI Genbank, and discovered that its sequence showed 100 % similarity values with the one of the five gray-faced buzzards which were previously researched. In addition, it was confirmed to be a female through sex determination using DNA. Such results are important information as it confirms the breeding of the gray-faced buzzards for the first time in 43 years since its breeding was last recorded in 1968, in Paju. Wildlife rescue center needs to work with adjacent consigned registration and preservation institutions when carcass of wild animals is collected or DNA samples are obtained for more accurate both species and sex identification through a systematic management system in the future. Furthermore, the obtained DNA sample of the gray-faced buzzard and COI gene, DNA barcode, could be used as reference standards for similar researches in the future.

Relationship between fatigue resistance and fracture behavior of the carbon fiber sheet and carbon fiber strand sheet reinforced RC slabs (Carbon fiber sheet 및 carbon fiber strand sheet 접착보강한 RC 상판의 내피로성과 파괴거동과의 상관관계)

  • Won, Chan Ho;Abe, Tadashi;Ahn, Tae-Ho;Kim, Do Keun
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.6
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    • pp.294-298
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    • 2015
  • According to the results of "Highway Bridges Long Life Repair Plan." The most serious damage to RC slabs is caused by fatigue deterioration, which results from the driving loads of large-sized vehicles, and aging of materials. In response to this, adhesion reinforcement using carbon fiber sheet is being adopted. In addition, carbon fiber strand sheet that holds the same material characteristics as CFS, but has superior workability, has been developed as a new reinforcement material. However, almost no studies have been conducted on CFSS in relation to fatigue resistance evaluation through fatigue tests under running wheel loads, with the exception of a few by some organizations. Therefore, in this study, specimens with front CFS adhesion reinforcement on the bottom surface of the RC slab and specimens with grid-type CFSS reinforcement were manufactured. Then, fatigue tests under running wheel loads were conducted, and thus fatigue resistance was evaluated using the specimens.

Presence of Carbonic Anhydrase III-like Protein in Shaggy Sea Raven, Hemitripterus villosus (삼세기(Shaggy sea raven, Hemitripterus villosus)의 carbonic anhydrase III에 관한 연구)

  • Kweon, Rok Eun;Kho, Kang Hee
    • Journal of Life Science
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    • v.24 no.2
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    • pp.186-190
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    • 2014
  • Carbonic anhydrase isozymes (CAs) are widespread zinc-containing metalloenzyme family. The enzyme catalyzes the reversible interconversion of $CO_2$ and $HCO_3$. This reaction is the main role of CA enzymes in physiological conditions. CA III, one of the CA isozymes, has been identified in many tissues. It is distinguished from the other isozymes by several characteristics, particularly by a lower specific activity and by its resistance to acetazolamide. However, the physiological function of CA III in fish is unknown. In this study, we examined the detection of CAs in the Shaggy sea raven Hemitripterus villosus, using SDS-PAGE, isoelectric focusing (IEF), and western blot analysis. We detected a significant protein band with molecular weight about 30 kDa from the tissues of H. villosus by SDS-PAGE and western blotting. A specific band of CA III with pI 7.0 was detected by IEF and western blotting in gill and muscle. The immunoreaction of anti-CA III expressed in the gill of H. villosus was much stronger than other tissues. One explanation for this result is that the fish gill is the only organ that is exposed to the external environment and that plays an important role in acid-base relevant ion transfer, the transfer of $H^+$ and/or $HCO{_3}^-$, for the maintenance of systemic pH. This is the first report on the identification of a carbonic anhydrase III-like protein from H. villosus.

Primary Surgical Closure of Large Ventricular Septal Defects in Small Infants (조기 영아기 심실 중격 결손의 개심술)

  • 최종범;양현웅
    • Journal of Chest Surgery
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    • v.30 no.5
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    • pp.486-492
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    • 1997
  • We r viewed a policy of primary surgical closure of large ventricular septal defects in small infants. Sixty-three infants met criteria for inclusion in the study, and were divided into two groups based on age: group 1 infants aged less than 5 months(n = 31), and group 2 infants aged 5 months or more(n = 32). Both groups had similar variation in ventricular septal defect location(paramembranous versus muscular), and showed no significant difference in left to right shunt and in ratio of systemic and pulmonary vascualr resistance. Three early deaths(9.7%) occurred in group 1, but no death(0%) in group 2. The causes of death were preoperative cardiac arrest and cerebral injury followed by postoperative respiratory insufficiency in two patients, and preoperative tracheomalacia followed by laryngeal edema and respiratory arrest in one Two patients in group 1 showed postoperative low cardiac output syndrome(6.5% in group 1 versus 0% in group 2). There was no late death during the follow-up per od in both groups. No surviving patients had postoperative patch leakage, or required a second operation. These results indicate that primary surgical closure of large ventricular septal defects, if logical perioperative care is accompanied, can be saefly performed in small infants aged less than 5 months with low postoperative mortality or morbidity rates.

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A Case Report of Heart-Lung Transplantation (심장-폐 이식 증례 보고)

  • 노준량;허재학;오삼세;김영태;이정렬;이기봉;오병희;한성구
    • Journal of Chest Surgery
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    • v.31 no.10
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    • pp.1004-1008
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    • 1998
  • We report a case of heart-lung transplantation in a 32 year-old female with Eisenmenger syndrome secondary to patent ductus arteriosus. She has been suffered from congestive heart failure since June 1996 and repeatedly treated at Intensive Care Unit with intravenous inotropic support since July 1997. Preoperative echocardiography showed a patent ductus arteriosus with right to left shunt, severe regurgitation of tricuspid valve and estimated right ventricular systolic pressure of 100mmHg. The brain-dead donor was an 18 year-old male with head trauma from traffic accident 3 days ago. Heart-lung block procurement was performed at another general hospital and was transported to the Seoul National University Hospital by ambulance. Total ischemic time of the transplanted heart and lung were 249 minutes and 270 minutes, respectively. The immunosuppressive therapy was commenced preoperatively with cyclosporine and azathioprine. Corticosteroid was not used until postoperative 3 weeks in order to avoid infection and delayed healing at the tracheal anastomotic site. The patient was discharged at 31st postoperative day, and has been regularly followed up at outpatient clinic without specific complication. The follow-up bronchoscopy, performed 2 weeks and 4 months after surgery, revealed no evidence of cellular rejection.

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The Modern Cultural Heritage Value and Conservation of Documentary Art Records (행정박물의 근대문화유산적 가치와 보존방향)

  • Kang, Dai-Ill
    • Journal of Conservation Science
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    • v.26 no.1
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    • pp.69-76
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    • 2010
  • As the tangible evidence, documentary art records are permanent records to reflect the historical, symbolical, cultural and artistic value. Also, documentary art records are related with mission fulfillment of public institution and it is documentary with administrative, cultural and artistic values as the works of documentary production and application of the administrative image. "The public records management law" was the first time that administration documents were incorporated and in the act No. 3 of administrative records management, the preservation and management system of documentary art records became legislated. The management of documentary art records are difficult to categorize only by the reflect of characteristics or materials. Because documentary art records are collected in many ways and made with materials. It needs to be collected with many informations on basic materials and with the appropriate conservation and management category. Documentary art records do not have lots of demands but the level of consciousness is improving and the possibility of opening to public is increasing for improvement with the right to know. In near future, the documentary art records will be used for the interpretation of historical and cultural preservation and modern cultural heritage could have the value as it is expected. Primarily to prevent deterioration on documentary art records the standard survey card should be made to categorize the damage rate and to check the status on each documentary art records. In addition, establishing a systematic management plan for various documentary art records are needed with the training of professionals for the conservation of documentary art records.

A Study on Present State of Preservation of Excavated Remains in Chungcheongnam-do and Preservation Methods (충청남도 발굴유구 보존현황 및 보존방안 연구)

  • Wi, Koang-Chul;Oh, Seung-Jun;Jung, Je-Won
    • Journal of Conservation Science
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    • v.33 no.6
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    • pp.541-552
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    • 2017
  • All excavated "buried cultural properties" are supervised by the Cultural Heritage Administration that decides whether to conserve them or not, depending on the necessity for conservation. Currently, there is no legal regulation concerning the administration of relics (including artifacts) and remains (including archaeological sites) that are relocated and restored based on such conservation decisions. Thus, administration of these cultural properties is inadequate. Under these circumstances, this paper recommends the current state of conservation and conservation methods for buried cultural properties excavated at Chungcheongnam-do thus far. Conservation of buried cultural properties excavated at Chungcheongnam-do primarily employ the "relocating and restoration" method; 23 relics and 46 remains employed relocation after dismantling and soil layer transcription method. Moreover, 27 remains were relocated after dismantling and 14 remains employed the transcription method. In terms of area, Buyeo-gun and Gongju-si had 12 relics accounting for about 50%, while according to the period, Baekjae period had 16 relics, accounting for about 70%. In terms of conservation facility, open-air facility had 33 relics, accounting for about 72%. Results of investigating the state of relics' management indicated that public institutions like a museum with a recognizable management system had their own work force and consequently ensured excellent conservation, whereas if there were no apparent responsible bodies, remains were subjected to considerable damage due to mismanagement. Therefore, this problem can be resolved by identifying a recognizable management body as well as ensuring periodical management and supervision. The excavated buried cultural properties are utilized not only for academic purposes, but also for PR or as exhibition material. Hence, this study confirms that meticulous management of buried cultural properties is currently required.

Fatal plastic bronchitis with eosinophilic casts in a previously healthy child (건강하였던 소아에서 발생한 치명적인 호산구성 증식성 기관지염)

  • Cho, Young Kuk;Oh, Soo Min;Choi, Woo-Yeon;Song, Eun Song;Han, Dong-Kyun;Kim, Young-Ok;Ma, Jae Sook
    • Clinical and Experimental Pediatrics
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    • v.52 no.9
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    • pp.1048-1052
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    • 2009
  • Plastic bronchitis is a rare disease characterized by the recurrent formation of branching mucoid bronchial casts that are large and more cohesive than those that occur in ordinary mucus plugging. Casts may vary in size and can be spontaneously expectorated, but some require bronchoscopy for removal. Plastic bronchitis can therefore present as an acute life-threatening emergency if obstruction of the major airways occurs. Three of 22 reported patients with eosinophilic casts were fatal, with death due to central airway obstruction. Here, we report a child with no history of atopy, allergy, or congenital heart disease who was diagnosed with plastic bronchitis with eosinophilic casts. Although he was administered intravenous (iv) antibiotics; iv corticosteroids; and a vigorous pulmonary toilet regimen, including chest physiotherapy and routine bronchoscopic removal of casts, he had brain death secondary to hypoxic brain damage. Plastic bronchitis can be fatal when casts obstruct the major airways, as in the present case. Clinicians should intervene early if a patient exhibits signs and symptoms consistent with plastic bronchitis.