• Title/Summary/Keyword: 기간분리

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Changes in Chromatographic Fractionation and Composition of the Proteins of Malting Barley Grain during Germination (발아기간별 맥주맥 단백질의 분획 및 조성변화)

  • Seo, Ho-Soo;Cho, Sung-Hwan
    • Korean Journal of Food Science and Technology
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    • v.24 no.1
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    • pp.1-6
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    • 1992
  • Changes in protein distributiun, eletrophoretic patterns and amino acid composition were investigated during germination of malting barley. Fractionation of the protein complex in ungerminated malting barley resulted in a higher hordein fraction but less glutelin fraction of the protein complex in ungerminated malting barley resulted in a higher hordein fraction but less glutelin fraction as compared to germinated malting barley. As germination proceeded, NPN, globulin and glutelin fractions continued to increase, accmpanied by decreases in albumin and hordein fractions. The electrophoretic pattern of malting barley proteins showed three bands (molecular weight range of $15,000{\sim}41,000$ daltons) in albumin fraction, five bands ($19,000{\sim}61,000$ daltons) in globulin fraction, five bands ($18,000{\sim}56,000$ daltons) in hordein fraction and tour bands ($20,000{\sim}47,000$ daltons) in glutelin fraction, exhibiting quantitative changes in each fraction during germination. Amino acid analysis showed that glutamic acid, histidine, aspartic acid, serine, glycine, valine, alanine and leucine were major amino acids of proteins in malting barley grains. Glutamic acid increased slightly, but other amino acids showed no definite trend as germination proceeded.

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Effect of Rifampicin on the Biosynthesis of Nucleic Acid in Chloroplast isolated from Chlorella ellipsoidea (Chlorella 세포에서 분리한 엽록체의 핵산합성에 미치는 rifampicin의 영향)

  • 이종삼;정희숙
    • Korean Journal of Microbiology
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    • v.24 no.3
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    • pp.276-287
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    • 1986
  • Chlorella ellipsoidea were cultured in the media containing rifampicin for 7 days. Aliquot cells were taken out after the inoculation and at intervals during cultivation and growth rate of Chlorella cells was measured. In order to investigate the effect of rifampicin on the nucleic acid synthesis, nucleic acid and RNA polymerase were extracted from chloroplast isolated from these cells, and the contents of nucleic acid and activity of enzyme were measured to compared with those of the control. The inhibitory concentration of rifampicin on growth was 80 ppm. The DNA contents in chloroplasts isolated were decreased 60% to compared with control, whole cells were markedly decreased 70% by rifampicin. The contents of base in the RNA were decreased 46% by rifampicin in shole cell, and 77% of base contents were decreased in chloroplast. Rifampicin also inhibited the activity of RNA polymerase, therefore whole cell was decreased 10% of activity and chloroplasts were decreased 42% of activity.

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염절임 가지(시키부, Shikibu)의 저장방법에 따른 성분 변화

  • 남학식;홍주연;김남우;엄영철;신승렬
    • Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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    • 2003.10a
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    • pp.195-195
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    • 2003
  • 본 연구는 수출방법의 개선과 절임 가공식품의 개발하고자 일본 수출용 시키부(Shikibu) 염절임 가지의 저장방법과 저장중에 당류, 유기산 및 아미노산의 변화를 분석하였다. 총당 및 환원당 함량은 저장방법에 관계없이 저장기간이 길어질수록 조금씩 감소하였고, 침지 저장한 것보다는 진공포장하여 저장한 것이 변화가 적었다. 가지에서 분리 정량된 유기산은 acetic acid, citric acid, lactic acid, malic acid 그리고 succinic acid 총 5종이었으며 acetic acid와 malic acid의 함량이 가장 높았으며, 저장기간이 길어질수록 저장 초기에 비해 acetic acid의 함량은 증가하였는데 반해 malic acid의 함량은 감소하였다. 가지의 주요한 구성아미노산은 valine, leucine, aspartic acid, glutamic acid, proline, alanine 등이었으며, 필수아미노산 함량은 저장기간이 길어질수록 저장 초기에 비해 진공포장하여 저장한 방법에서는 감소하였다가 증가하였는데 반해 침지하여 저장한 방법에서는 계속 증가하였고 저장 20일째에는 진공포장한 방법보다 l00mg/100g 정도의 차이를 나타내었다. 유리아미노산은 aspartic acid, alanine, cystine과 proline등의 함량이 높았고, 저장 기간이 길어질수록 저장초기에 비해 유리아미노산의 함량이 증가하였는데, 특히 진공 포장하여 저장한 가지에 비해 침지 저장 한 가지에서 그 함량이 높았다. 또한 아미노산 유도체의 함량은 phosphoserine, taurine, ${\gamma}$-aminoisobutyric acid 및 hydroxyproline의 함량이 높았고, 저장중에 phosphoserine의 함량은 감소하였는데 반해 ${\gamma}$-aminoisobutyric acid는 진공포장하여 20일 동안 저장하였더니 생가지 보다 5배 이상의 증가를 보였다. 스키부품종의 절임가지의 저장방법과 저장기간에 따른 성분의 변화를 고려할 때 축양품종과 동일하게 진공포장하는 것이 절임가지의 품질유지할 수 있는 것으로 생각된다.

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진탕배양에 의한 Monascus sp.가 생산하는 적색색소에 관한 연구 (제1보) 균주의 분리 및 색소생산 배양조건의 검토

  • 김현수;김두현;양호석;변유량;유주현
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1978.10a
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    • pp.206.3-206
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    • 1978
  • 태국으로부터 발효원료 수입된 Tapioca chips에 부착된 사상균과 곡자, 공기, 식물의 잎으로부터 적색색소를 생산하는 미생물을 분리하여 그 배양조건을 검토하였다. 분리한 균수는 Eumycetes중 균사에 septa를 가지는 Ascomycetes과의 Monascus sp.로 동정되었다. 최적 적색색소 생산의 배지조성분은 탄소원으로 Tapioca chips powder 3.5%, 질소원으로 $NaNO_3$ 0.2%, amino acid 중 L-arginine, L-glutamic acid, L-proline 0.3%, vitamin 중 folic acid, niacin 1 $\mu\textrm{g}$/mι. 무기염류로 $MnO_2$ 0.001% 첨가가 효과적이었고 배양온도 $32~33^{\circ}C,$ pH 6.5, 배양기간 4일, 배지용량 100/500mι, 진탕배양(180rpm)의 조건하에서 적색색소 생산이 잘 되었다.

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제어망 보안을 위한 일방향자료전달시스템의 송수신 에이전트 개발

  • O, Yeong-Cheol;Han, Mi-Ran;Sin, Yong-Tae;Kim, Jong-Bae
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.05a
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    • pp.708-709
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    • 2015
  • 최근 외부의 악의적인 공격으로부터 내부의 시스템을 보호하기 위하여 논리적, 물리적으로 망을 분리하고 있다. 하지만 사회공학적 해킹에는 물리적 망 분리도 취약할 수밖에 없다. 이러한 이유로 국가기반시설들을 담당하는 주요 기관들은 좀 더 안전한 네트워크 망을 구성할 필요가 있다. 따라서 본 논문에서는 일방향자료전달 시스템을 제안한다. 본 논문에서 제시한 일방향자료전달시스템은 제어시스템으로부터 업무망으로 전달되어지는 정보를 수신하여 처리하고, 업무망의 제어 정보는 송신되지 않도록 구성한다. 이 방식을 통해 어떠한 경우에도 외부로부터 내부의 제어망을 통해 기간시스템에 접근하는 것이 불가능하기 때문에 국가기반시설을 안전하게 보호할 수 있다.

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Photosynthetic Characteristics of Intact Cells and Thylakoid Membranes of Synechococcus PCC7002 with Polyvinyalcohol-Immobilization (Synechococcus PCC7002의 세포 및 틸라코이드 막의 Polyvinylalcohol 고정화에 의한 광합성 특성)

  • 윤지은;전현식
    • KSBB Journal
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    • v.8 no.2
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    • pp.185-191
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    • 1993
  • Highly stable $O_2$-evolving cells and thylakoid membranes have been obtained from the cyanobacterium, Synechococcus PCC7002, by immobilization with polyvinylalcohol(PVA). The absorption peak showed the blue-shift of about 3 nm after immobilization of intact cells and thylakoid membranes as well as isolation of thylakoid membranes. Photosynthetic electron transport activities, especially PS II activity showed greater stability in the PVA-immobilized cells and thylakoid membranes when stored at $4^{\circ}C$ than in those at $25^{\circ}C$. When the cells were threated at higher temperature, the level of Fo and Fv increased. After imobilization, however, Fo showed no change. This suggests that the immobilization can protect against the damages of PS II complex, especially a water-spiliting system, by heat treatment.

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Chemical Composition of the Rice Germ from Rice Milling and its Oil Stability during Storage (쌀 도정시 분리되는 쌀눈의 일반성분과 저장 중 유지 안정성)

  • Shin, Dong-Hwa;Chung, Chong-Ku
    • Korean Journal of Food Science and Technology
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    • v.30 no.1
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    • pp.241-243
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    • 1998
  • The chemical composition of rice germ, a by product of rice milling, was analyzed and its oil stability was evaluated by acid value and Rancimat method during storage. The predominent components of the germ were carbohydrate (36.5%). crude lipid (21.6%) and crude protein (18.7%). The time taken to double the acid value was 2 days for common rice germ, while it took 31 days to increase 1.15 times for rice germ dried at $80^{\circ}C$ for 6 hours. Induction periods of the lipid oxidation from common or dry germ stored at different temperature were not significantly different.

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Separated Control Signaling Protocol for WDM Optical Networks (파장 분할 다중화 방식을 사용하는 광 전송망을 위한 분리 제어 신호 방식)

  • 소원호;김영천
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.37 no.6
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    • pp.361-361
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    • 2000
  • In this paper, we propose a novel control signaling protocol that efficiently controls connection setup and increases the utilization of network resources. The proposed protocol, Separated Control Signaling Protocol(SCSP), separates bearer control from call control lot WDM optical networks. The main function of call control is to check the availability of network resources such as wavelengths and receivers at destination node. Bearer control is to reserve and assign wavelengths. The signaling architecture of this protocol consists of call controller and hearer controller The call controller handles call setup and release, activates the beater controller, and manages the status of call and bearer. The bearer controller reserves wavelengths, sets up bearer, tears down bearer. and notifies the status of beater to call controller. The state transition diagrams of each controller are designed. Using control messages and related primitives, the information flows for call setup and bearer setup, hearer teardown and call release, and reaction for setup failures are described to evaluate the performance. The simulation results show that the separated control signaling protocol is superior to conventional one in terms of call blocking probability and resource utilization.

Identification of Antioxidative Components from Ethanol Extract of Rhus verniciflua STOKES (옻나무 에탄올 추출물로부터 항산화 활성 물질의 구조동정)

  • Kim, In-Won;Shin, Dong-Hwa;Baek, Nam-In
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1654-1660
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    • 1999
  • The free phenolic acid fraction of the chloroform extract from 75% ethanol extract of Rhus verniciflua STOKES (RCF) showed stronger antioxidative activity than BHT, BHA and ${\delta}-tocopherol$ at the same concentration. RCF components were isolated and identified by silica gel column chromatography, thin layer chromatography, mass spectrometer and $^1H-NMR\;and\;^{13}C-NMR$. The antioxidative activity was confirmed by electron donating activity, Rancimat method and thiobarbituric acid test in liposome system. RCF-11 could be further separated into three fractions. The antioxidative active compounds were purified and identified as gallic acid, butin and butein. The RCF-13 was purified and identified as sulfuretin.

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Yogurt Production Using Exo-polysaccharide-producing Leuconostoc and Weissella Isolates from Kimchi (김치유래 exo-polysaccharide 생성능 Leuconostoc 및 Weissella균을 이용한 발효유 제조)

  • Min, Koung-Ah;Chung, Chang-Ho
    • Korean Journal of Food Science and Technology
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    • v.48 no.3
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    • pp.231-240
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    • 2016
  • The purpose of this study was to investigate the effect of exopolysaccharide (EPS)-producing Leuconostoc and Weissella isolates from kimchi as a probiotic starter and replacement for thickening agents such as pectin and gums in yogurt. Potential probiotic isolates were first screened for their acid and bile tolerance, and then evaluated for antimicrobial activity against Escherichia coli and Salmonella Typhimurium. When the selected Leuconostoc or Weissella isolates were co-inoculated in yogurt without a thickening agent, the yogurt with 4% sucrose produced lower syneresis values than the control and had higher EPS yields. The isolates were able to survive at a level of $10^6CFU/mL$ when incubated at $4^{\circ}C$ for 12 days. This study shows that EPS-producing Leuconostoc and Weissella strains have the potential to produce a synbiotic yogurt.