• Journal of fish pathology
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• v.1 no.2
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• pp.131-134
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• 1988

Radiocobalt was used as tracer to study the uptake of cobalt by fish either from water or food source, respectively. Four groups of goldfish were exposed to $^{57}Co$-labelled water under 4 different temperatures, while another four groups were fed labelled feed pellets at $15^{\circ}C$. Results indicate that water temperature has no significant effect on the uptake of cobalt from water. The uptake from water is quite a slow process since the cobalt uptaken from water reached 0.0006% of available activity at best on the 13th day of exposure. The uptake from a labelled feed source showed a linear increase with time over the period of ninety days. On the 90th day of experiment the percentage of available activity consumed seemed to be approaching an equilibrium point at roughly 12% of the total activity fed to the goldfish.

• Parasites, Hosts and Diseases
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• v.25 no.1
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• pp.69-82
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• 1987

The prevalences of the cuke belonging to genus Metagonimus hove been reported along the upper stream of inhabitants by several workers since 1980, however the taxonomical problems of the fluke was not yet settled. The larval flukes; cercaria and metacercaria as well as their intermediate hosts, and adult were studied in order to identify the Mepagonimus in the areas. The results obtained are summarized as follows: 1. The snails, Semisulcospira globus were collected (rom the three different localities along the upper stream of the River. The cercariae were found from 125(7.2%) out of 1,730 snails by natural emerging method, and were identified into 5 species including Metagenimus sp. (3.7%), Pseudexorchis major(1.4%), Cercaria nipponensis (0.9%), Cercaria incerpa(0.6%), and Cercaria yoshidae(0.6%). Cercariae of Metagonimus species had four to dye oral spines on its anterior of the first line. 2. The cercariae of Metagonimus were experimentally exposed to goldfish. nfection rate was 22.9% out of 105 goldfish, and the encysted metacercariae were found in fins(86.7%) and on scales (13.7%) of the fishes, but not in their muscle, head or visceral organs. 3. Seven species of ask were caught in the Daecheong Reservoir and the upper stream. Infestations with metacercaria of Metagonimus were found 100% in Opsariichtys widens and the parasitized numbers of the metacercariae were observed from 250 to 2,400 per fish. In the upper stream, Zacco termmincki, Z. platypus and Pseudogobio esocinus were infected 100% with the metacercaria, on the other hand, the fishes caught in the reservoir showed the lower infestation rates, and a few metacercariae found in the fishes Carassius carassius and Cyprinus carpio in the reservoir and the stream. The majority of metacercariae was detected only on the scales of fishes. 4. In order to know the infectivity and the distribution patterns in the intestine of hosts, rats and dogs were infected with the metacercariae obtained from O. bidens and Z. platypus. In addition the metacercariae obtained from Z. temmincki, P. esocinus and goldfish were given to the rats. The recovery rates of the worms in the small intestine of dogs were higher (63.3~65.8%) than those of the rats (3.5~31.6%). The flukes were found mostly in the middle and the lower part of small intestine of the rats and the dogs, but no worm was collected in the upper part of the intestine of rats. 5. The sixte of adult flukes varied by the hosts. In the adult cukes, oral sucker was smaller than ventral sucker, and the right and left testes were located diagonally, the uterine tubules circled around the upper left testis. The average egg sixte was $29.1{\times}1.7{\mu\textrm{m}}$. According to the above results, the cukes belonging to genus Metagonimus distributed along the Geum River was concluded to be identical with Miyata type of M. yokogawai as that Saito had proposed.

• Applied Microscopy
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• v.16 no.2
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• pp.35-48
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• 1986

Ultrastructures of the cutaneous xanthophores and formation of pigment granules in the goldfish, Carassius auratus L., are studied with electron microscope. The cutaneous xanthophores are observed only in dermis and pigment granules of these pigment cells are pterinosomes and carotenoid vesicles. By the differentiated level, pterinosomes are subdivided into 3 types; while type I pterinosomes have clear limiting membranes and contain some amorphous fine fibrous structures, type II pterinosomes have thick and densely aggregated fibrous materials. Type III pterinosomes have concentric lamellar structures in the granules. Pigment granules of the xanthophores are originated from the Golgi complexes and pinocytotic vesicles of plasma membrane as well as rER-rich cells among the chromatophores are presumed to be associated with the accumulation of pigment materials.

• Applied Microscopy
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• v.24 no.1
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• pp.1-10
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• 1994

The integumental secretory structure is exocrine unicellular gland located in the epidermis of goldfish, Carassius auratus, and divided into two groups, mucous and granular cells. By the histochemical studies of integumental secretions the mucos cells reacted for acidic polysaccharides, and the granular cells for neutral glycoprotein. According to concentration of the secretion the integumental mucous are gradually sulphated. The mucous cells are typical form of goblet cell located in the upper region of the epidermis, and membrane bounded vesicles of the mucous are observed several size and electron densities by the cellular differentiation. The granular cells in middle and lower epidermis are present syncitial forms occasionally, and contain electron dense granules sized $1.0{\mu}m$ which are accumulated in cytoplasmic process held the cells to the basal lamina. The precursors of the integumental secretory materials are originated from the rough endoplasmic reticulum and next transported through the Golgi apparatus as a form of membrane bounded vesicles. After accomplish this process mature secretions are extruded to integumental surface by the mechanism of merocrine secretion in response to nerve stimulations respectively.

• Applied Microscopy
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• v.17 no.1
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• pp.65-75
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• 1987

The ultrastructure of the cutaneous chromatophores and the origin of pigment granules in the goldfish, Carassius auratus L., are studied with electron microscope. Leucophores contain amorphous pigment granules, leucosomes and melanosomes contain oval shaped electron dense melanin pigment granules. These pigment granules are synthesized from the Golgi complexes of each chromatophores and released into the cytoplasm. Among the dermal chromatophores, another kind of dermal cells, named rER rich cells, are also observed. And pinocytotic vesicles are being formed only the inner surface of the chromatophores adhering to the rER rich cells. From these observations, such hypothesis are being possible that pigment materials are synthesized and transfered from the rER rich cells to dermal chromatophores by the pinocytosis. And next remodelizing mechanisms are carried out by the Golgi complexes of each chromatophores.

• Journal of fish pathology
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• v.16 no.2
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• pp.125-129
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• 2003

Conncxin (Cx) is an important and essential protein induction of oocyte maturation, which is present in almost all mammalian tissucs except cirulating blood cells and adult skeletal museles. In this study, goldfish Cx43 cDNA sequence is available in GenBank under the accession number AB078505. Homology analyses using the GenBank and EMBL general database searches indicated that goldfish Cx43 cDNA has a high homology with carp Cx43 (95.1% identity), zebrafish Cx43 (90.5% identity), and chicken Cx43 (81.9% identity). Goldfish Cx43 is similar to the Cx family in its general features, and all the typical Cx consensus sequences are also found. Moreover, significantly increased Cx43 transcrpts were observed in mature goldfish (GSI; 18.3-21.7) pituitary and ovary when compared with immature goldfish (GSI; 4.9-6.0). Cx43 transeripts were weakly detectrd in both liver and kidney of immature and mature goldfish. There is possible that Cx43 nctivity was relation to oocyte maturation in the goldfish.

• Korean Journal of Veterinary Research
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• v.34 no.2
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• pp.369-374
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• 1994

The acute and subacute toxicity of fungicide folpet was evaluated in fingerings of common carp, Cyprinus carpio and goldfish, Carrassius auratus. Dipping of fishes for acute toxicity was performed for a period of 24h, and the TLm value(median tolerance limit) was 1.52 ppm in common carp and 1.45 ppm in goldfish. Severe damages were observed in various organs and among them, clubbing of gill lamella, lytic degeneration and vacuolation of liver cells, and epithelial edema of renal tubules were relatively prominent. The most significant changes were hyperbasophilic foci of liver cells in subacute toxicity test and these can imply the possibility of hepatocarcinogenecity of folpet.

• The KIPS Transactions:PartB
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• v.8B no.1
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• pp.50-58
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• 2001

본 논문에서는 움직임을 갖는 다중 물체 중에서 하나의 물체를 선택하고, 선택된 물체를 계속 추적하는 알고리즘을 제안하였다. 일반적으로 차영상을 이용하는 이동물체의 동작정보 추출방법은 주로 연속되는 영상내에서 일정한 영역의 영상특성을 정합하는 방법이 주로 사용되어왔다. 본 논문에서 제안한 동작정보의 추출방법은 연속영상간의 차연산을 한번 시행함으로써 얻어진 움직임영역을 기반으로 사용자에 의해 임의로 선택된 특정한 움직임영역을 추출하는 것이다. 특정한 모양이나 패턴을 인식하여 추적하는 것과는 달리 본 논문에서는 사용자에 의해 선택된 물체를 추적목표물체로 삼는 새로운 이동추적 방법을 제시하였다. 실험은 CCD 카메라로 입력된 실제 금붕어 영상을 이용하여 선택된 이동물체의 추적이 효과적으로 수행됨을 보였다.

• Korean Journal of Microbiology
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• v.22 no.2
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• pp.85-90
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• 1984

Two viruses were isolated from kidney and spleen tissues of goldfish(Carasius auratus) and the ovarian fluid of chum salmon(Oncorhynchus keta). Both viruses replicated and produced cytopathic effect in EPC, CHSE-214, and CHH-1 cell lines at $15^{\circ}C$. The isolates were resistant to pH 3 and choloroform. Antiserum to infections pancreatic necrosis virus(IPNV) serotype VR 299 neutralized the infectivity of both of the isolates. Electron microscopy showed that the particles had typical IPNV particle morphology with average diameters of 55nm, This paper describes the first isolation of viruses infecting cultured fish in Korea.

• Journal of fish pathology
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• v.3 no.1
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• pp.21-25
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• 1990

In inland aquaculture, a lot of fishes often died cause of touch of L. carteri which is attached on the net cage. In this works, the experiment were carried out on classification of species, toxin test, and extermination with some chemicals. A bryozoa from net cage in lake Okjeong is classified into the Phylactolaemata, Lophopodella carteri Hyatt. A bryozoa broke out firstly on the 30th of June with water temperature up to $31^{\circ}C$ and it reattached on the net with water temperature below $30^{\circ}C$. Size of bryozoa colony is about $0.8{\times}0.6{\sim}1.7{\times}1.5cm$. Water column of attachment was 0~5m and the peak is 2~3m. In toxin test on the israeli carp, goldfish and catfish touched with bryozoa for 1 minute at $25^{\circ}C$ of water temperature, a dead fish appeared at 20 minutes after touched. Mortality was 90% for israeli carp and 100% for goldfish at 100 minuties after touched, but catfish was not died at all. It was supposed that the toxin is from nematocyst being around tentacles and this toxin act a deadly poison on a israeli carp and goldfish. In extermination test, the bryozoa treated with 300ppm of formalin, 5% of sodium chloride, 5ppm of malachite green, 200ppm of potassium permanganate, 1000ppm of potassium iodide, 10ppm of DDVP for minute respectively, all of them were not effected.