• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.511-516
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• 2010

The process to acquire intron-GUS gene-expressed transformants from somatic embryos (including embryogenic calli) of Rosa hybrida cv. 'Sweet Yellow' using Agrobacterium-meditated transformation method was reported in this study. Somatic embryos including embryogenic calluses were infected with Agrobacterium tumefaciens AGL1 strain (O.D = 0.7~1.6) including intron-GUS gene for 30 min, and were co-cultured for 3 days. After co-cultivation, they were cultured on embryo germination medium (EGM) supplemented with $250\;mg{\cdot}L^{-1}$ cefotaxim at $4^{\circ}C$ for 7 days. Then, transient GUS gene expression was observed. Shoots were regenerated from the shoot primodia induced from the intron-GUS gene-transferred either somatic embryos or embryogenic calli cultured on EGM supplemented with both cefotaxim $250\;mg{\cdot}L^{-1}$ and ppt $2\;mg{\cdot}L^{-1}$. Before induction of rooting from shoots cultured on shoot growing medium supplemented with both cefotaxim $250\;mg{\cdot}L^{-1}$ and ppt $2\;mg{\cdot}L^{-1}$, the shoots were cultured on multi-shoot induction medium supplemented with both cefotaxim $250\;mg{\cdot}L^{-1}$ and ppt $2\;mg{\cdot}L^{-1}$ to induce multi-shoots. When expression of the gene from a part of the multi-shoots was identified by GUS transient assay, the putative transgenic multishoots were transferred to rooting medium supplemented with cefotaxim $250\;mg{\cdot}L^{-1}$. After the formation of healthy roots, transgenic plantlets were transferred to the greenhouse after acclimatization. The expression rate of the intron-GUS gene in the multi-shoots was 100%.

• Journal of KIISE:Software and Applications
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• v.28 no.3
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• pp.224-238
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• 2001

본 논문은 ATM(Abstract Timed Machine)으로 명세된 실시간 시스템을 검증하기 위한 방법을 기술한다. ATM은 임무 위급 시스템인 실시간 시스템을 명세, 분석, 검증하기 위한 정형기법이다. ATM은 모드와 전이, 포트로 구성되어 있으며 모드는 머신의 압축된 상태를 표현한다. 전이는 하나의 모드에서 다른 모드로의 전환을 나타내며 조건과 이벤트로 구성되어 있다. 포트는 ATM간의 상호작용을 위한 진입을 표현한다. 다른 정형기법과 비교하여 ATM은 소프트웨어의 순환공학 과정에서 사용하기 위해 설계되었다. 역공학 측면에서 볼 때 ATM은 계산 논리뿐만 아니라 실시간 시스템의 실제 소스코드에 있는 설계나 환경정보를 표현할 수 있다. 이러한 목적을 위해 ATM의 모드는 계산모드, 추상화 모드, 주제모드로 구분된다. 계산 모드는 코드 상에서의 논리와 계산을 나타내며 추상화 모드는 모드와 전이의 블록을 하나의 ATM으로써 표현한다. 대개의 경우, 이것은 코드 상에서의 블록을 ATM내 하나의 모드로 나타낼 때 사용한다. 주제 모드는 예외나 주기적 동작 등과 같은 다수의 ATM의 주제를 표현한다. 실시간 시스템을 검증하기 위해 시스템의 소스 코드는 역명세 과정을 통하여 ATM으로 표현된다. 검증은 ATM에 대한 도달성 그래프를 생성하는 것에 의해 수행된다. 도달성 그래프는 상태와 시간을 추상화되고 압축된 형태로 표현할 수 있으며 그 결과 시간 속성을 지닌 상태 공간을 감소시킬 수 있다. 또한 시스템의 교착상태를 쉽게 발견할 수 있다. 본 논문은 ATM과 실행 모델, 도달성 그래프, 검증을 위한 속성 등을 기술하며 이들을 다른 정형 방법들과 예제를 통하여 비교한다.수 있다. 모피우스는 헤더나 광고와 같은 불필요한 정보들을 제거하는 별도의 단계를 거치지 않으므로 wrapper를 빠르게 생성한다. 궁극적으로 모피우스는 새로운 웹 상점을 사용자가 자유롭게 추가, 삭제할 수 있는 환경을 제공한다.X>와 반응시킬 경우에는 반응식 c에 의거 진행됨을 예측할 수 있었다.의거 진행됨을 예측할 수 있었다.이 거의 산화되지 않았고, $700^{\circ}C$에서도 ZnS와 ZnO 상이 공존한 것으로 보아 SnO$_2$코팅이 ZnS의 산화를 억제하는 것으로 나타났다.pplied not only to the strike system in the RSC circle, but also to the logistics system in the SLC circle. Thus, the RSLC model can maximize combat synergy effects by integrating the RSC and the SLC. With a similar logic, this paper develops "A Revised System of Systems with Logistics (RSSL)" which combines "A New system of Systems" and logistics. These tow models proposed here help explain several issues such as logistics environment in future warfare, MOE(Measure of Effectiveness( on logistics performance, and COA(Course of Actions)

• Journal of the Korean Geotechnical Society
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• v.37 no.12
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• pp.47-56
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• 2021

The cryosuction (negative pore pressure) in freezing soils causes groundwater migration from the frozen fringe to freezing front for ice lens formation. Frost heave and heaving pressure by ice lens cause damage to ground infrastructure. In order to prevent damage by the frost heave, various frost susceptibility criteria have been proposed. The SP (Segregation Potential) is the most widely used classification criterion for frost susceptibility in cold regions. The expansion of the ice lens by the migration of the groundwater is a key role in frost heave mechanism, and thus it is necessary to evaluate the hydraulic conductivity. In this paper, soil mixtures of coarse-fines (sand-silt) were prepared in various weight fractions and used for frost heave and column permeability test. For each case, the SP and the hydraulic conductivity were derived and correlations were analyzed. As a results, the transition threshold of the SP and the hydraulic conductivity were shown at 20% and 50% of the silt weight fraction, respectively. Although there are difference between these transition thresholds, these two coefficients show a specific correlation. In the future, additional study should be conducted for detailed analysis of the threshold transition values between SP and hydraulic conductivity.

• The Journal of the Korean bone and joint tumor society
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• v.20 no.1
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• pp.32-35
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• 2014

Rosai-Dorfman disease (RDD) is an idiopathic histioproliferative disorder of lymph node and extranodal site. Bone involvement is very rare. We report a case of extranodal RDD of the tibia in 32-year old male. The patient presented with pain with no evidence of lymphadenopathy. Clinico-radiologic diagnosis was metastatic carcinoma or Langerhans cell histiocytosis, but, histopathologic examination confirmed the diagnosis with RDD. We performed curettage on the osteolytic lesion of tibia. In South Korea, there was no report about RDD of the extremity and we want to report this case with review of the literature.

• The Journal of the Korean bone and joint tumor society
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• v.2 no.1
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• pp.72-77
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• 1996

Metastaic bone tumors are usually accompanied with severe pain. The treatment modalities for this pain are so variable that patients are sometimes afraid of using them. Salmon calcitonin has a function to increase beta-endorphines followed by increasing the blood level of prostaglandin and thromboxan A2, which results in analgesic effect. This drug also has been known to decrease bone resorption. There were a few reports that parenteral use of salmon calcitonin decrease the pain from metastatic bone tumor. We wanted to know the effectiveness and tolerability of nasal spray of salmon calcitonin in relieving bone pain with metastatic tumor. We analyzed the effectiveness in the aspects of pain, sleep, performance status, mobility, supplementary analgesic use. The biologic effect of salmon calcitonin was analysed with CBC, Ca/P, BUN/Cr, uric acid. Simple radiography, alkaline phosphatase, osteocalcin, pyrilink-K were used as parameters for bone change. Eighteen cases of metastatic bone tumors took nasal spray of salmon calcitonin($Miacalcic^{(R)}$, 200IU/day) for 4 weeks, to relieve bone pain. With Wilcoxon Matched-Pairs Signed Ranks Test, we could find pain decreased significantly at 3 week and mobility become improved at 4 week of salmon calcitonin use. Other parameters didn't show any significant changes. We think the analgesic effect is mainly due to effect not on the local bone lesion but on the central nervous system, and that increased dose of salmon calcitonin can induce earlier and stronger analgesic effect.

• Journal of Chest Surgery
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• v.30 no.2
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• pp.146-151
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• 1997

Between December 1993 and March 1996, 75 patients had undergone ubannular procedures in mitral valve reconstruction. Their mean age was 45 years and they consisted of 28 males and 47 females. The cause of mitral disease in 75 patients were classified as follow : 29 cases were degenerative, 40 cases were rheumatic, 3 cases were congenital, 2 cases were infectious and 1 case was ischemic. Average number of mitral anatomical lesion per patient was 3.1 and we used average 1.5 procedures on subannular structure in mitral valve per patient. Subannular procedures were chordae shortening 21, chordae transfer 22, new chordae formation 20, papillary muscle splitting 33, shortening of papillary muscle 2. Intraoperative transesophageal echocardiography was carried out for providing an immediate and accurate assessment of the adequacy of the reconstruction. There was no operative death. Patients have been followed up from 2 to 29months, mean 12.5. There were two failures that necessitated reoperation. The mean functional class (NYHA) was 3.19 preoperative and improved to 1.12 postoperatively. After mitral valve repair, the patients were improved hemodynamic, echocardiographic data. In conclusion, subannular procedure in mitral valve repair when feasible is stable and safe with a low prevalence of reoperation.

• Radiation Oncology Journal
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• v.23 no.2
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• pp.111-115
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• 2005

Primary malignant melanoma of the vagina is an extremely rare genital neoplasm occurring mainly on postmenopausal women. It has a worse prognosis than cutaneous melanomas, because of the high rate of loco-regional recurrences and rapid systemic dissemination. In the past, radical surgical extirpation as the primary management had been recommended to improve loco-regional control, and possibly overall survival. However, the prognosis was poor In spite of such a radical approache. Recently, more conservative treatment such as wide local excision combined with adjuvant high-dose fraction radiotherapy seems to have premising results. Primary radiation therapy could be served as an alternative to surgery for patients with lesion less than 3 cm in diameter. We report 2 cases of primary vaginal malignant melanoma treated with radiotherapy.

• Journal of Digestive Cancer Research
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• v.3 no.2
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• pp.108-112
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• 2015

A 54-year-old male patient who was diagnosed with advanced gastric cancer underwent a distal gastrectomy, D2 lymph node dissection, and adjuvant chemoradiotherapy. After a year, in a follow-up PET-CT, lymph node metastases were observed in the neck and abdomen, and therefore, the patient underwent chemotherapy. After treatment, the follow-up PET-CT revealed a growth of the posterior neck lymph node. Thus, an excisional biopsy was performed, and the growth was diagnosed as metastatic adenocarcinoma. Therefore, the patient received chemotherapy with FOLFIRI. Another follow-up PET-CT after chemotherapy revealed a growth in the right inguinal lymph node, and the patient underwent salvage radiotherapy for this lesion. The PET-CT taken for the response evaluation showed no evidence of further metastasis of the lymph node. We hereby report a case of advanced gastric cancer with neck and inguinal lymph node recurrence showing complete remission after palliative chemotherapy and salvage radiotherapy.

• Journal of Digestive Cancer Research
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• v.6 no.1
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• pp.40-44
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• 2018

A 77-year-old man presented with abdominal discomfort and was diagnosed as Borrmann type 3 advanced gastric cancer with multiple lymph node metastases. An abdominal computed tomography (CT) and positron emission tomography-computed tomography (PET-CT) showed AGC, clinical stage IIIC (T4aN3M0). We started neo-adjuvant chemotherapy with FOLFOX (5-fluorouracil (5-FU))+Leucovorin+Oxaliplatin). After 3 cycles of FOLFOX chemotherapy, follow-up endoscopy showed remarkable improvement. Primary lesion and metastatic lymph nodes decreased size on follow up computed tomography (CT). The patient underwent radical total gastrectomy with esophagojejunostomy and histopathology revealed no remnant malignant cells at previous primary cancer lesion. The patient has currently completed his 3 cycle of adjuvant chemotherapy without recurrence. After an abdominal CT response assessment, further course of therapy will be decided.

• Journal of the Korean earth science society
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• v.22 no.5
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• pp.377-387
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• 2001

In order to elucidate sedimentation processes and depositional environments in transitional area between continental shelf and coastal zone, sedimentologic study has been done with 84 surface sediments sampled in nearshore/offshore off the mid-western coast of the Korean Peninsula for 3 years (1996${\sim}$1999). The surface sediment can be classified into 4 facies (gravelly sand, sand, silty sand and sandy silt). Mean grain size, sorting, skewenss and kurtosis varies -0.39${\sim}7.82{\Phi}$, 0.36${\sim}4.68{\Phi}$, -0.38${\sim}$0.86, -1.56${\sim}$3.43, respectively. The textural parameters show a finer-grained and poorly-sorted trend shoreward, northward and southward from the central part of the study area. The positively-skewed distribution and relationship of each textural parameters indicate a tide-dominated depositional environment. According to C/M diagram, there are 3 different domains (mode A, B, C) of sediment transport mode. The northern part is characterized by bedload transport (mode A) and represents co-influence of wave and tide, whereas domain C in the southern part is controlled by uniform suspension transport (mode C), correlating with sandy-silt area. In the broad middle area, transport processes are complex (the mixture of bedload, graded suspension and uniform suspension; mode B). Hence, the subdivision depositional environments of this study area may be classified by 3 depositional environments dependent on the interplay of sediment supplies from river, relict sediments and hydrologic conditions. In results, the nearshore and offshore areas are thus characterized as a mixing zone between coastal terrigenous sediments and relict sediments in the continental shelf by complex processes (tide, wave and river flow). These sedimentation processes play an important role in producing distinct sedimentologic features in the transitional zone linking coastal and shelfal areas.