• Journal of the Korea Organic Resources Recycling Association
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• v.23 no.1
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• pp.70-81
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• 2015

Helicases are known to be a proteins that use the chemical energy of NTP binding and hydrolyze to separate the complementary strands of double-stranded nucleic acids to single-stranded nucleic acids. They participate in various cellular metabolism in many organisms. DEAD-box proteins are ATP-dependent RNA helicase that participate in all biochemical steps involving RNA. DEAD-box3 (DDX3) gene is belonging to the DEAD-box family and plays an important role in germ cell development in many organisms including not only vertebrate, but also invertebrate during asexual and sexual reproduction and participates in stem cell differentiation during regeneration. In this study, in order to identify and characterize DDX3 gene in the earthworm, Perionyx excavatus having a powerful regeneration capacity, total RNA was isolated from adult head containing clitellum. Full length of DDX3 gene from P. excavatus, Pe-DDX3, was identified by RT-PCR using the total RNA from head as a template. Pe-DDX3 encoded a putative protein of 607 amino acids and it also has the nine conserved motifs of DEAD-box family, which is characteristic of DEAD-box protein family. It was confirmed that Pe-DDX3 has the nine conserved motifs by the comparison of entire amino acids sequence of Pe-DDX3 with other species of different taxa. Phylogenetic analysis revealed that Pe-DDX3 belongs to a DDX3 (PL10) subgroup of DEAD-box protein family. And it displayed a high homology with PL10a, b from P. dumerilii.

• Korean Journal of Medicinal Crop Science
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• v.14 no.3
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• pp.125-129
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• 2006

Two herbal medicines of the Polygonatum genus, namely Polygonati Rhizoma and Polygonati Odorati Rhizoma, are difficult to distinguish from each other through exterior morphologic aspects. Furthermore, because the standard components for physiochemical distinction have not yet been standardized, the identification of these medicines through botanic taxology is based on genetic methods of random amplified microsatellite polymorphism (RAMP). For the RAMP evaluation, we used five sets of UBC microsatellite primers 811, 818, 834, 836, 842 and random primer M1. Although no specific band that could clearly distinguish Polygonati Rhizoma from Polygonati Odorati Rhizoma was found, 11 Polygonatum plants could be divided into two groups by this method. P. sibiricum and P. stenophyllum were classified to group I and the others were to group II. As P. sibiricum and P. stenophyllum were very similar in genetic and morphologic perspective, the results suggest that P. stenophyllum belongs to the Polygonati Rhizoma family.

• Journal of Adhesion and Interface
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• v.3 no.2
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• pp.9-16
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• 2002

In the synthesis of water-based polyurethane using self-emulsification process, after being neutralized, polyurethane pre-polymers containing ionic pendant groups are dispersed by simple convective mixing. Preparation of dispersion is followed by chain extension reaction, which is considered as important step for growth of the molecular weight of polyurethane. In this work, pH variations in the aqueous phase were monitored during the chain extension process in the presence of water-soluble diamines. The optimum degree of chain extension and the average particle size in the polyurethane dispersions were examined with varying ionic pendent group contents, type of chain extenders, and feed rate of chain extenders, The initial pH value in the aqueous phase linearly increased and the optimum chain extension point could be obtained from the intersection of two linear lines having different slopes, All average particle sizes before chain extension reaction were almost same, however, the final average particle size increased as feed rates of chain extenders increased, In addition, as the ionic pendant group contents increased, the particle size decreased since the hydrophilicity and hydrodynamic volume increased. As carbon numbers of the chain extenders increased, the final particle size increased significantly. From the results, it was concluded that the chain extension reaction took place among the particles not only in a particle.

• Microbiology and Biotechnology Letters
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• v.51 no.1
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• pp.93-98
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• 2023

Nuruk is a starter culture of Korea manufactured by spontaneous fermentation of grains. We isolated bacteria of the genera Bacillus and Staphylococcus, and lactic acid bacteria (LAB) from eight commercial nuruk samples collected from four districts of Korea using selective agar media and identified them based current taxonomic standards. Bacillus was detected in all samples, but Staphylococcus or LAB were not detected in three samples. In seven samples, except one sample scored the highest cell number of LAB, Bacillus and Staphylococcus were counted as the highest and the lowest numbers, respectively. Six species of Bacillus were identified, and B. subtilis, B. velezensis, and B. licheniformis were predominant species. Nine species of coagulase-negative Staphylococcus were identified, and the predominance of S. pseudoxylosus and S. saprophyticus was confirmed. Ten species of LAB including Enterococcus, Lactobacillus and close relatives, Pediococcus, and Weissella were identified. P. pentosaceus was identified as the predominant species.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.12
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• pp.1094-1101
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• 2010

Microbial fuel cells (MFC) were enriched using sediment Nakdong river, Hoidong river and protected water area in Gijang. The microbial community of sediment and enriched MFC was analyzed by FISH (fluorescent in situ hybridization) and 16S rDNA sequencing. ${\alpha}$-Proteobacteria, Acidobacter and Cyanobactia group were dominant in sediment by FISH. The coulombs of the final 10 peak of the 3 MFC (Nakdong, Hoidong, Gijang) were 0.64 C, 0.50 C, 0.61 C, respectively. When MFCs were enriched by sediment, ${\beta}$-, ${\gamma}$-Proteobacteria, Acidobacter and Firmicutes group increased 45~90%, 50~90%, 40~80% and 45~125%, respectively. In results of 16S rDNA sequencing, Roseomonas sp., Azospillium sp., Frateuria sp., Dyella sp., Enterobacter sp. and Deinocossus were isolated from Nakdong river and Azospillium sp., Delftia sp., Ralstonia sp., Klebsiella sp. and Deinococcus sp. were isolated from protected water area in Gijang and Pseudomonas sp., Klebsiella sp., Deinococcus sp., Leifsonia sp. and Bacillus sp. were isolated from Hoidong river.

• Journal of KIISE:Databases
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• v.29 no.4
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• pp.247-261
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• 2002

Aggregation is an important operation that affects the performance of OLAP systems. In this paper we define a new class of aggregation queries, called range-groupby queries, and present a method for processing them. A range-groupby query is defined as a query that, for an arbitrarily specified region of an n-dimensional cube, computes aggregations for each combination of values of the grouping attributes. Range-groupby queries are used very frequently in analyzing information in MOLAP since they allow us to summarize various trends in an arbitrarily specified subregion of the domain space. In MOLAP applications, in order to improve the performance of query processing, a method of maintaining precomputed aggregation results, called the prefix-sum array, is widely used. For the case of range-groupby queries, however, maintaining precomputed aggregation results for each combination of the grouping attributes incurs enormous storage overhead. Here, we propose a fast algorithm that can compute range-groupby queries with minimal storage overhead. Our algorithm maintains only one prefix-sum away and still effectively processes range-groupby queries for all possible combinations of the grouping attributes. Compared with the method that maintains a prefix-sum array for each combination of the grouping attributes in an n-dimensional cube, our algorithm reduces the space overhead by (equation omitted), while accessing a similar number of cells.

• Proceedings of the Korean Fiber Society Conference
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• 2002.04a
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• pp.97-100
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• 2002

PVDF를 전장하에서 용융결정화 하는 경우에 전장의 세기가 증가할수록 전기적으로 불활성인 $\alpha$-결정의 핵생성속도는 지연되는 반면에 전기적으로 활성을 갖는 ν-결정의 핵생성은 빨라지는 것으로 Stein 연구그룹이 일찍이 보고한 바 있다. [1,2] 그러나 실제로 PVDF를 용응 결정화하는 경우에는 결정화온도가 그다지 낮지 않는 경우에는 $\alpha$-결정과 ν-결정이 혼재되어 나타나기 때문에 외부 전장하리서의 용융결정화시에 외부전장의 세기가 $\alpha$-결정과 ν-결정의 핵생성속도 및 성장속도에 미치는 영향을 분리하여 해석한 논문은 거의 없다. (중략)

• Proceedings of the Korea Contents Association Conference
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• 2014.11a
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• pp.205-206
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• 2014

인구 고령화는 세계적 추세이지만, 특히 한국과 일본은 현저하게 빠른 증가 속도로, 2050년 일본이 세계 1위, 한국이 세계 2위로 전망되고 있다. 본 연구는 한국과 일본의 고령자 일상생활활동의 건강성에 관한 실태와 비교 분석을 통해 사회, 문화 및 노인 인구 증가 속도 등 여러 면에서 우리나라와 유사점을 가지고 있으면서도 노인 문제를 우리보다 약 20년 정도 앞선 경험으로 고령화 대책이 구체적인 일본 노인복지를 통하여 한국 노인 문제에 다양한 시사점을 제시하고자 하였다. 이를 위해 한국과 일본 각각 양국가의 언어로 번역된 설문지를 사용하여, 한일 65세 이상 노인 603명과 267명을 연구대상으로 하였다. 훈련된 연구 조사원들을 통해 일대일 면접 방식으로 한국의 노인복지관과 노인대학과 센터 등에서, 일본의 시니어클럽연합회와 사회복지법인의 자원봉사 그룹, 고령자 생협 소속 노인들에게 조사되었다. 이를 통해, 행복한 노년기를 위한 인간존엄의 근원적 테마로 다양한 시사점을 제시하였다.

• Proceedings of the KSME Conference
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• 2001.06b
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• pp.488-494
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• 2001

Many literatures, so far, have concentrated on approaches employing dependent coordinates set resulting in computational burden of constraint forces, which is needless in many cases. Some researchers developed methods to remove or calculate it efficiently. But systematic generation of the motion equation using independent coordinates set by Kane's equation is possible for any closed loop system. Independent velocity transformation method builds the smallest size of motion equation, but needs practically more complicated code implementation. In this study, dependent velocity matrix is systematically transformed into independent one using dependent-independent transformation matrix of each body group, and then motion equation free of constraint force is constructed. This method is compared with the other approach by counting the number of multiplications for car model with 15 d.o.f..

• Proceedings of the Korean Information Science Society Conference
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• 2007.06b
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• pp.32-35
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• 2007

Bioinformatics의 목표는 생물학적인 질의를 해결하는 것과 생물학자들이 수집된 데이터를 분석하고 검색을 하여 생물학자들이 정확한 일을 수행하는 것이다. 인터넷은 여러 조사 그룹의 데이터베이스에 동시에 접근가능한 수단을 제공했으나 이러한 분산 환경에서 많은 양의 데이터는 전송 시의 시간 지연 문제와 최종 검색시의 느린 검색 속도 문제를 나타낸다. 데이터 클러스터링은 데이터의 검색시 이러한 문제점을 해결하기 위하여 이용될 수 있는 방법이지만 단순 적용시에는 데이터의 양에 비례하는 실행 시간이 또 다른 문제를 발생시킨다. 본 논문에서는 바이오데이터의 효율적인 클러스터링을 위한 개선된 분산 클러스터링 시나리오와 이를 위해 수정된 K-means 알고리즘을 제시한다. 최종 실험 결과는 20% 이상 향상된 실행 속도를 보여준다.