• Korean Journal of Poultry Science
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• v.37 no.4
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• pp.355-360
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• 2010

To estimate the genetic characteristics within two brands of Korean native commercial chicken, we used a total of 302 genomic DNAs from two groups (Woorichicken: 152, Hanhyup3chicken: 150). Sizes of 10 microsatellite markers were decided using GeneMapper Software (v.4.0) after analyzing ABI 3130. Genetic diversity indices including expected heterozygosity (Ex H), observed heterozygosity (Ob H) and polymorphism information content (PIC). Frequencies of microsatellites markers were used to estimate heterozygosities and genetic distances. LEI0073 showed the highest value in all genetic diversity (Ex H, Ob H and PIC). On the other hand, MCW322 showed the lowest value in all genetic diversity. The calculated genetic distance of the two brand groups is 0.199 (standard genetic distance) and 0.132 (DA distance). Genetic distances of the two groups were relatively close to each other. Each individual is ramified to two brand groups in phylogenetic dendrogram.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.3
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• pp.319-325
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• 1999

A culture-dependent survey of bacterial community in the soil-root system of red pepper and tomato was conducted by dilution plate count method. The bacterial community within soil was not different from that of rhizoplane. However, the populations of fluorescent, pseudomonads were higher in rhizoplanes than in soils and higher in healthy rhizoplanes than in Phytophthora disease-infested rhizoplanes. The bacterial community of the pepper cropped soil and rhizoplanes was very similar to that of the tomato-cropped soil and rhizoplanes. Among 285 identified bacterial colonies, most colonies were belong to two groups by fatty acid analyses: 52% of the 285 colonies were belong to low G + C gram positive bacteria group. Bacillus spp. and 33% were belong to high G + C gram positive bacteria group. In order to use beneficial microorganisms to agro-ecosystem, these data of field trials should be intensively accumulated.

• Korean Journal of Microbiology
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• v.46 no.1
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• pp.73-79
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• 2010

The Korea Polar Research Institute (KOPRI) has assembled a culture collection of cold-adapted bacterial strains from both the Arctic and Antarctic. To identify excellent protease-producers among the proteolytic bacterial collection (874 strains), 78 strains were selected in advance according to their relative activities and were subsequently re-examined for their extracellular protease activity on $0.1{\times}$ ZoBell plates supplemented with 1% skim milk at various temperatures. This rapid and direct screening method permitted the selection of a small group of 15 cold-adapted bacterial strains, belonging to either the genus Pseudoalteromonas (13 strains) or Flavobacterium (2 strains), that showed proteolytic activities at temperatures ranging between $5-15^{\circ}C$. The cold-active proteases from these strains were classified into four categories (serine protease, aspartic protease, cysteine protease, and metalloprotease) according to the extent of enzymatic inhibition by a class-specific protease inhibitor. Since highly active and/or cold-adapted proteases have the potential for industrial or commercial enzyme development, the protease-producing bacteria selected in this work will be studied as a valuable natural source of new proteases. Our results also highlight the relevance of the Antarctic for the isolation of protease-producing bacteria active at low temperatures.

• The Korean Journal of Mycology
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• v.32 no.1
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• pp.1-7
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• 2004

To establish the phylogenetic relationships of Dictyophora spp., rDNA-ITS regions of 11 strains of veiled lady mushroom collected from various countries were amplified and sequenced. It was observed that the 11 strains were divided into four groups based on PCR band patterns of each ITS region cleaved by eight different restriction enzymes in cleaved amplified polymorphic sequence analysis (CAPS). The phylogenic relationship of each group by cleaved amplified polymorphic sequence (CAPS) analysis matches well with previously reported morphological phylogeny, such as 5 strains of D. indusiata, 4 strains of D. echinovolvata, and a strain of Phallus rugulosus. Sequence analysis using the cluster V methods showed more detail classification than CAPS analysis. The 5.8S region showed two point nucleotide base exchanges from G to A according to four groups, and four groups were subdivided by sequence variation of ITS I and ITS II regions. But sequence variation of Phallus rugulosus was not showed in full ITS region. This study further delineates the taxonomic level at which ITS sequences, in comparison to ribosomal gene sequence, are most useful in systematics and other mushroom study.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.141-147
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• 1993

Plasmid DNAs were detected from the mitochondrial fraction of four strains of whiterot fungus, Pleurotus ostreatus. The size of the plasmids were 10.2 and 7.2 kb in strain NFFA 2, 10.2 kb in NFFA 4001, 11.2 kb in NFFA 4501, and 10.2 and 11.2 kb in KFCC 11635. The two strains,NFFA 2ml and NFFA 2m2, which are mutant derivatives of NFFA 2, did not contain any plasmids. The cleavage by proteinase K indicated that these plasmids have DNA ends associated with proteins. In digestion with proteinase K all the plasm ids remained resistant to lambda exonuclease which hydrolyzes DNA from 5' ends and were sensitive to exonuclease III which hydrolyzes DNA from 3' ends. This suggests that the plasmids are linear double-stranded DNA and the terminal proteins are covalently linked to 5' ends of plasm ids. In order to find relationship between these plasmids, hybridization of plasm ids by each separate plasmid DNA was done. The result indicated that the plasmids can be classified into at least 3 groups. Plasmids of group I were present in all the P ostreatus. More mitochondrial plasmids were detected in P cornucopiae. P ,florida, P pulmonarius, P sajor-caju, and P spodoleucus. The size of plasmids ranged between 7.2 kb and 14 kb. All the species except P cornucopiae contained plasmids of approximately 10 kb which hybridized with the 10.2 kb plasmid (group I) of P ostreatus NFFA 2.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.13 no.1
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• pp.177-185
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• 2009

Cluster analysis or clustering is a kind of unsupervised learning method in which a set of data points is divided into a given number of homogeneous groups. Fuzzy clustering method, one of the most popular clustering method, allows a point to belong to all the clusters with different degrees, so produces more intuitive and natural clusters than hard clustering method does. Even more some of fuzzy clustering variants have noise-immunity. In this paper, we improved the Possibilistic Fuzzy C-Means (PFCM), which generates a membership matrix as well as a typicality matrix, using Gath-Geva (GG) method. The proposed method has a focus on the boundaries of clusters, which is different from most of the other methods having a focus on the centers of clusters. The generated membership values are suitable for the classification-type applications. As the typicality values generated from the algorithm have a similar distribution with the values of density function of Gaussian distribution, it is useful for Gaussian-type density estimation. Even more GG method can handle the clusters having different numbers of data points, which the other well-known method by Gustafson and Kessel can not. All of these points are obvious in the experimental results.

• Journal of Korean Society of Forest Science
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• v.103 no.2
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• pp.189-195
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• 2014

Basic leucine zipper (bZIP) protein is a regulatory transcription factor that plays crucial roles in growth, development and stress response of plant. In this study, we isolated a PagbZIP1 gene that belonged to Group SE3 of bZIP from Populus alba ${\times}$ P. glandulosa, and investigated its expressional characteristics. The PagbZIP1 is 844 base pairs long and encodes a putative 144-amino-acid protein with an expected molecular mass of 16.6 kDa. The PagbZIP1 has two conserved domains including the basic and leucine zipper portions. Southern blot analysis revealed that two copies of the gene are presented in the poplar genome. PagbZIP1 was specifically expressed in the root and suspension cells. Moreover, the expression of PagbZIP1 was induced by drought, salt, cold and ABA. Therefore, our results indicated that PagbZIP1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in poplar.

• The KIPS Transactions:PartB
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• v.10B no.4
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• pp.381-388
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• 2003

This paper presents a method of giving weights to garbage class clustering and Filler model to improve performance of keyword spotting system and a time-saving method of dialogue speech processing system for keyword spotting by calculating keyword transition probability through speech analysis of task domain users. The point of the method is grouping phonemes with phonetic similarities, which is effective in sensing similar phoneme groups rather than individual phonemes, and the paper aims to suggest five groups of phonemes obtained from the analysis of speech sentences in use in Korean morphology and in stock-trading speech processing system. Besides, task-subject Filler model weights are added to the phoneme groups, and keyword transition probability included in consecutive speech sentences is calculated and applied to the system in order to save time for system processing. To evaluate performance of the suggested system, corpus of 4,970 sentences was built to be used in task domains and a test was conducted with subjects of five people in their twenties and thirties. As a result, FOM with the weights on proposed five phoneme groups accounts for 85%, which has better performance than seven phoneme groups of Yapanel [1] with 88.5% and a little bit poorer performance than LVCSR with 89.8%. Even in calculation time, FOM reaches 0.70 seconds than 0.72 of seven phoneme groups. Lastly, it is also confirmed in a time-saving test that time is saved by 0.04 to 0.07 seconds when keyword transition probability is applied.

• Journal of Conservation Science
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• v.26 no.1
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• pp.95-107
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• 2010

Biochemical research was carried out on 4 human skeletal remains from historical lime-layered tombs assigned to the Joseon Dynasty in Oknam-ri, Seocheon. The preservation of femur was evaluated by stereoscopic microscopy and scanning electron microscopy. Most of specimens showed good histological preservation. The histological results proved to be a good potentiality for biochemical analysis using bio-molecules. The amelogenin gene and mitochondrial DNA (mtDNA) analyses revealed that three specimens perhaps have maternal consanguinity due to sharing with mtDNA haplogroup D4b1, and two specimens buried in the same tomb were a couple in Gatjaegol site. Carbon and nitrogen stable isotope analysis indicated that four deads diet were built around C3 plant as rice, barley, wheat and bean. In this study we characterized genetic and diet features from the social stratum who could make lime-layered tombs during period of the Joseon Dynasty. The results suggest that biochemical research using the human skeletal remains from the Joseon Dynasty has the great potential and reasonable value for archaeology, anthropology, and population genetics.

• 한국방재학회:학술대회논문집
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• 2011.02a
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• pp.89-89
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• 2011

도로의 가드레일 지주 근입깊이의 부족에 의한 자동차의 전락사고 이 후, 일본의 국토교통성 등의 관계자들이 그 대책 세우기에 부심해 왔으나, 기설 지주의 근입깊이를 측정할 수 있는 방법은 아직까지 알려져 있지 않으며, 현재로서는 작업의 전 과정을 비디오로 촬영하여 그 기록을 남기도록 되어있다. 그러나 그것은 상당히 비효율적인 작업으로 엄밀한 감시기능을 다하지 못하고 있으며, 감독자와 시공자의 양자로부터 계측 도구의 개발이 절실히 요구되고 있다. 일부의 초음파 측정기 업자가 가드레일 지주의 근입깊이를 측정할 수 있다고 주장하고 있으나, 시장에는 아직 나타나지 않고 있으며, 그 측정시스템의 측정여부와 성능의 검증이 이루어지지 않고 있는 상황이다. 지금까지 충격탄성파법 또는 초음파법을 이용하여, 매설된 가드레일 지주의 근입깊이를 측정한 성공사례가 정식으로 보고된 바는 없으며, 같은 강관주인 눈사태 방지책의 지주 파이프에 대한 근입깊이의 측정은 본 연구그룹의 의해 행하여진 바가 있다. 검사봉이나 해머 등으로 대상물을 두드려서 탄성파를 발생시키고, 그것을 가속도계 또는 속도계의 진동센서로 감지하여 그 파형을 분석함으로써 대상물의 치수 등을 측정하는 충격탄성파법은, 특히 콘크리트를 대상으로 공동 및 매설물 등의 탐사, 균열깊이의 측정 등에 폭 넓게 사용되고 있다. 하지만 이 측정방법을 가드레일의 지주의 근입깊이 측정에 적용할 경우, 일반적으로 행하여지는 방법, 즉 진동센서를 대상물의 상단부(캡)에 설치하는 방법으로는 접합부에 의한 탄성파의 손실과 캡의 휨 진동에 의한 노이즈 등을 해결하기가 곤란해진다. 또한 지반의 존재로 인한 진동 모드의 변화와 진동에너지의 감소 등의 문제점을 해결하지 않으면 안 된다. 본 연구는 충격탄성파법을 이용하여 지반에 설치된 눈사태 방지책이나 가드레일의 지주와 같은 강관 구조물의 근입깊이를 측정하고자 하는 연구이다. 이를 위해 진동센서를 캡이 아닌 측면부에 취부장치를 이용하여 설치함으로써 길이방향의 탄성파를 측정할 수 있도록 하고, 실제 구조물에 대해 측정을 실시하여 그 측정시스템의 성능과 유용성을 검토하고자 한다. 또한 다양한 길이의 실험용 강관 파이프를 매설하고 측정실험을 실시하여 측정시스템의 적용성에 대해서도 검토하였다. 본 연구를 통하여, 수신센서를 파이프의 측면에 선접촉하게 함으로서 종파를 감지하여 근입깊이를 포함한 파이프의 전 길이를 측정하는 본 측정시스템은 매설된 강관 구조물의 길이 측정에 기본적으로 적용 가능함을 확인할 수 있었다. 특히 오거 굴착으로 시공된 경우에는 높은 정도의 측정성능을 보여주었다. 또한 항타관입 파이프에 대해서는 지반의 영향을 고려함으로써 길이의 측정이 가능하다는 것을 확인할 수 있었다. 즉, 오거 굴착 또는 항타 관입 등 시공방법에 따라 측정결과에 대한 지반의 영향 정도가 달라지며 파형 분석 및 길이 산정시 그 영향을 고려하여야 함을 확인하였다.