• Journal of the Korean Electrochemical Society
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• v.6 no.1
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• pp.41-47
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• 2003

For the development of low temperature anode-supported planar solid oxide fuel cell, the planar anode supports with the thickness of 0.8 to 1 mm and the area of 25, 100 and $150\;cm^2$ were fabricated by the tape casting method. The strength, porosity, gas permeability and electrical conductivity of the planar anode support were measured. The porosity of anode supports sintered at $1400^{\circ}C$ and then reduced in$H_2$ atmosphere was increased from $45.8\%\;to\;53.9\%$. The electrical conductivity of the anode support was $900 S/cm\;at\; 850^{\circ}C$ and its gas permeability was 6l/min at 1 atm in air atmosphere. The electrolyte layer and cathode layer were fabricated by slurry dip coating method and then had examined the thickness of $10{\mu}m$ and the gas permeability of 2.5 ml/min at 3 atm in air atmosphere. As preliminary experiment, cathode multi-layered structure consists of LSM-YSZ/LSM/LSCF. At single cell test using the electrolyte layer with thickness of 20 to $30{\mu}m$, we achieved $300\;mA/cm^2$ and 0.6V at $750^{\circ}C$

• Journal of Periodontal and Implant Science
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• v.30 no.2
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• pp.213-231
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• 2000

이 연구의 목적은 다공성의 CPP 내부에 쥐의 장골의 골수에서 유래된 세포를 접종하고 3차 원적으로 배양하여 CPP가 골 형성을 위한 조직공학의 지지체로 적용가능한가를 연구하는 것과 Calcium PolyPhosphate(CPP)의 돌연변이 유발성을 검사하는 것이다. 무수 ($Ca(H_2PO_4)$)를 condensation하여 무결정의 ($Ca(PO_3)$)를 얻고 이를 용융하고 냉각시킨 후 분쇄하여 Calcium polyphosphate(CPP) powder를 얻었다. 다공성의 CPP는 5% $SiO_2$를 첨가하여 sponge 형태로 $450-550{\mu}m$ 소공의 크기를 가지는 것과(CPP-45ppi) $200-300{\mu}m$의 소공의 크기를 가지는 것(CCP-60ppi) 2가지로 제작하였다. 각각의 CPP matrices는 $5mm{\times}5mm{\times}1mm$의 블록 형태로 만들었다. 체중 100g 내외의 백서에서 장골(femur, tibia)을 채취하여 백서의 장골 골수 세포를 분리하여 배양한 후 24well에 CPP block을 넣고 CPP block 당 $10^5$개의 배양한 세포를 접종하였다. 배양 1, 7, 14, 및 21 일째에 각 well에서 trypsin EDTA를 이용하여 2회 반복하여 cell을 분리하였고, 원심분리한 후 hemacytometer로 측정하였다. 또, 45ppi와 60ppi, 그 리 고 Tissue Culture Polystyrene(control group)에 접종, 배양된 세포들의 염기성 인산분해효소활성도를 배양 7, 14, 및 21 일째에 각각 측정하였다. 각 기간별로 배양된 세포-CPP 혼합체내에서 세포의 부착 및 증식과 형성된 조직의 3차원적 형태를 관찰하기 위하여 주사전자현미경하에서의 관찰하였다. CPP의 돌연변이 유발성 검사 (mutagenicity test)를 위해 hypoxanthine-guanine phosphoribosyl transferase(HPRT) assay를 하였다. NIH3T3 cell line과 CHO-K1 cell line으로 각각 $1000{\mu}g/m{\ell}$, $100{\mu}g/m{\ell}$, $10{\mu}g/m{\ell}$ 그리고 $1{\mu}g/m{\ell}$의 CPP 농도에서 측정하였다. 통계적 분석을 위해서 모든 측정은 각군당 4개체 이상 시험하였고, 각 측정값은 평균값${\pm}$표준편차로 나타내었다. 각 군간의 통계적 유의성 검정을 위해서 Analysis of variance(ANOVA)를 이용하였고 Tukey의 방법으로 사후분석을 실시하였다. 제작된 CPP matrices 소공들이 서로간에 연결이 잘 되어있는 형태였다. 두 가지로 제조된 CPP(45ppi와 60ppi) 모두에서 세포의 부착이 잘 일어났고, 부착된 세포의 분열도 잘 일어났다. 2 가지의 CPP 모두에서 7, 14, 21일째의 세포 수는 1일째에 비해 유의성 있게 증가하였다(P<0.01). 3차원적 구조인 Calcium PolyPhosphate에서 배양한 세포는 24well dish(tissue culture polystyrene)에서 평면적으로 배양한 대조군의 세포에서 보다 염기성 인산분해효소 (Alkaline Phosphatase)를 유의성 있게 높게 나타냈다. 주사전자현미경에서 세포-CPP 혼합체를 관찰한 결과, CPP block에 세포들이 잘부착되어 있었고, 시간이 지남에 따라 세포가 여러 층을 형성하면서 뭉치는 현상을 보였다. 또, HPRT assay 결과 , Calcium PolyPhosphate는 돌연변이 유발성을 보이지 않았다. 이상의 결과로 볼 때 CPP에는 세포부착이 잘 일어나고, 지지체 상에서 세포의 분열도 활발하게 일어나므로 골조직을 위한 조직공학의 우수한 지지체가 될 수 있을 것으로 사료된다.

• Journal of Soil and Groundwater Environment
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• v.9 no.1
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• pp.79-86
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• 2004

Hydraulic conductivity along rock fracture is mainly dependent on fracture geometries such as orientation, aperture, roughness and connectivity. Therefore, it needs to consider fracture geometries sufficiently on a fracture model for a numerical analysis to calculate permeability coefficient in a fracture. This study performed new type of numerical analysis using a homogenization analysis method to calculate permeability coefficient accurately along single fractures with several fracture models that were considered fracture geometries as much as possible. First of all, fracture roughness and aperture variation due to normal stress applied on a fracture were directly measured under a confocal laser scaning microscope (CLSM). The acquired geometric data were used as input data to construct fracture models for the homogenization analysis (HA). Using the constructed fracture models, the homogenization analysis method can compute permeability coefficient with consideration of material properties both in microscale and in macroscale. The HA is a new type of perturbation theory developed to characterize the behavior of a micro inhomogeneous material with a periodic microstructure. It calculates micro scale permeability coefficient at homogeneous microscale, and then, computes a homogenized permeability coefficient (C-permeability coefficient) at macro scale. Therefore, it is possible to analyze accurate characteristics of permeability reflected with local effect of facture geometry. Several computations of the HA were conducted to prove validity of the HA results compared with the empirical equations of permeability in the previous studies using the constructed 2-D fracture models. The model can be classified into a parallel plate model that has fracture roughness and identical aperture along a fracture. According to the computation results, the conventional C-permeability coefficients have values in the range of the same order or difference of one order from the permeability coefficients calculated by an empirical equation. It means that the HA result is valid to calculate permeability coefficient along a fracture. However, it should be noted that C-permeability coefficient is more accurate result than the preexisting equations of permeability calculation, because the HA considers permeability characteristics of locally inhomogeneous fracture geometries and material properties both in microscale and macroscale.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2009.12a
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• pp.297-297
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• 2009

최근 유기농산물에 대한 관심과 요구가 급증하면서 소비자들은 고품질 유기농산물을 선호하고 있어 국내 대부분 유기농가들은 무처리 일반종자를 이용하고 있는 실정이다. 앞으로 국제 기준에 부합하는 채소류 유기재배를 위해서는 유기종자의 이용이 필수적이므로 유기종자 채종체계 확립이 필요한 실정이다. 따라서 본 연구에서는 당근 종자(재래종 heimloom)를 사용하여 유기종자의 채종체계를 확립하고자 하였다. 일반적으로 당근종자 생산은 적어도 2년을 요구한다. Seed to seed 방법과 Root to seed 방법이 이용되고 있는데 본 연구에서는 Root to seed 방법을 사용하였다. Root to seed 방법은 6월에 당근종자를 파종하여 가을에 뿌리를 수확하여 지상부를 5cm 정도 남기고 정지하여 일시 저온저장 하였다가 이듬해 뿌리를 이식하여 새순에서 추대시켜 종자를 수확하는 방법으로 종자 생산성이 높아 종자회사에서 많이 사용하고 있다. 본 연구에서도 Root to seed 방법으로 채종 시험하였다. 2008년도에 파종 및 수확한 뿌리를 저온저장고에 일시 저장 후 1차 이식은 2008년 11월 하순에 노지포장에서 실시하고 이때 월동 중 동해를 막기 위하여 보온덮개로 멀칭 처리하였다. 이듬해 3월 중순에 주간거리 75${\times}$40cm 간격으로 솎음하여 처리당 10주씩 배치하였다. 이식후 뿌리에서 새순 1대를 유도하였고 추대 및 개화 6주후부터 종자가 갈색으로 변하기 시작할 때 채종을 시작하였다. 수확부위는 1차 측지 중 1번째 화지를 대상으로 하였고 화경절단은 화총으로부터 20cm 정도 남겨서 잘랐고 실내에서 1개월 이상 충분히 후숙시킨 후 종자를 정선하여 종자생산성을 조사하였다. 결실율과 발아율 조사는 채종된 종자를 이용하여 종자 충실도와 발아력을 조사하였다. 각 품종별 추대율은 15번 품종과 16번, 24번 품종이 5% 미만으로 매우 부진하였고 그밖에 추대된 묘의 생육 및 발육상은 매우 양호하여 개화에는 커다란 문제가 없었다. 종자 결실율은 23번 품종을 제외한 모든 품종에서 높았고 각 품종별 화총구조를 고려해 볼 때 주간 화총, 1차측지, 2차측지 순으로 결실율이 떨어졌다. 각 품종의 천립중은 1, 11, 12, 14, 20번 품종이 2.02g 이상으로 대립종으로 판단되며 각 품종별 화총의 크기는 비슷하여 대립종은 각 화총당 종자수가 적은 것으로 나타났다. 대분분의 대립종은 만생종으로 생육기간이 소립종 보다 10-20일상 길며 종자 성숙기간도 장기간 소요되며 특히 장마기간을 넘겨야 할 경우에는 비가림 시설 채종재배가 필수적인 것으로 나타났다. 발아율은 10, 11, 14, 18, 19, 26번 품종이 80% 이상으로 가장 높았고 다른 품종에서는 다소 떨어져 품종별 차이가 많았다. 종자 생산성은 장근종과 단근종간에 약간의 차이가 있지만 주간 화총을 포함하여 1차 측지 4-5개에서 채종하는 것이 가장 효율적인 것으로 사료된다. 또한 당근의 개화 기간을 보면 1화륜은 8~10일, 1개체는 40~50일, 채종포장에서는 1화륜에서 측지 6개구까지 채종은 60~70일 소요되어 미숙종자와 과숙종자가 혼재되어 적정 수확시기를 결정하기가 매우 어려운 점이 발생하였다. 본 연구 결과에서 생육특성, 수량성, 품질특성, 종자 생산성, 발아율을 고려해 볼 때 1, 7, 8, 9, 10, 14, 19, 26번 품종이 유기농 재배 및 유기채종에 적합한 것으로 사료되었다.

• Journal of the Korean Wood Science and Technology
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• v.27 no.1
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• pp.37-49
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• 1999

The effect of fire-retardant treatment and redrying on the mechanical properties of radiata pine sapwood were evaluated. Small, clear specimens were treated with three different fire-retardant(FR) chemicals, borax-boric acid(BRX), minalith(MIN), and pyresote(PYR), with target retentions of 30 and 60kg/$m^3$, and then redried at maximum dry-bulb temperature of $25^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$ or $110^{\circ}C$. Each specimen, including untreated and water-treated controls, was tested in static bending and in compression parallel to grain. The extent of strength reduction was dependent on the type of FR chemicals, retention, and redrying temperature, and a highly significant interaction existed between FR treatment and redrying temperature. Modulus of rupture(MOR) and work to maximum load(WML) were significantly decreased by FR treatment and redrying. None of three FR chemicals adversely affect modulus of elasticity (MOE) and maximum crushing strength(MCS). MOE of BRX treatment and MCS of both BRX and PYR treatment increased significantly compared to untreated controls. No significant differences existed between retention levels except for MOE and MCS of some combinations of FR chemicals and redrying temperatures. Although MOE and MCS was not significantly affected by any of the redrying temperatures, these properties were generally decreased with the increase in redrying temperature. The significant reduction in MOR and WML was observed in BRX treatment when dried at temperatures of $60^{\circ}C$ and above, and in MIN and PYR treatment when dried at temperatures of $80^{\circ}C$ and above. Consequently, BRX-treated radiata pine should not be redried at temperatures >$60^{\circ}C$, and MIN- and PYR-treated radiata pine should not be redried at temperatures > $80^{\circ}C$ where bending strength and energy-related properties are important design considerations.

• Nuclear Medicine and Molecular Imaging
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• v.41 no.4
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• pp.299-308
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• 2007

Purpose: The aim of this study was to develop a bioinformatics software and to test it in serum samples of papillary thyroid cancer using mass spectrometry (SELDI-TOF-MS). Materials and Methods: Development of 'Protein analysis' software performing decision tree analysis was done by customizing C4.5. Sixty-one serum samples from 27 papillary thyroid cancer, 17 autoimmune thyroiditis, 17 controls were applied to 2 types of protein chips, CM10 (weak cation exchange) and IMAC3 (metal binding - Cu). Mass spectrometry was performed to reveal the protein expression profiles. Decision trees were generated using 'Protein analysis' software, and automatically detected biomarker candidates. Validation analysis was performed for CM10 chip by random sampling. Results: Decision tree software, which can perform training and validation from profiling data, was developed. For CM10 and IMAC3 chips, 23 of 113 and 8 of 41 protein peaks were significantly different among 3 groups (p<0.05), respectively. Decision tree correctly classified 3 groups with an error rate of 3.3% for CM10 and 2.0% for IMAC3, and 4 and 7 biomarker candidates were detected respectively. In 2 group comparisons, all cancer samples were correctly discriminated from non-cancer samples (error rate = 0%) for CM10 by single node and for IMAC3 by multiple nodes. Validation results from 5 test sets revealed SELDI-TOF-MS and decision tree correctly differentiated cancers from non-cancers (54/55, 98%), while predictability was moderate in 3 group classification (36/55, 65%). Conclusion: Our in-house software was able to successfully build decision trees and detect biomarker candidates, therefore it could be useful for biomarker discovery and clinical follow up of papillary thyroid cancer.

• Applied Biological Chemistry
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• v.45 no.2
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• pp.71-76
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• 2002

Several antagonistic bacteria, SD-1, 4, 10, 11, 14, 15, and 16, which have strong CMCase and amylase activities, were isolated from the fermented mushroom media. Among them, SD-1, 10, 11, and 15 have strong antibacterial activities against the mushroom pathogenic bacteria Pseudomonas sp., and SD-1, 10, 11, 14, and 16 have strong antifungal activities against the mushroom pathogenic fungi, Trichoderma sp. SD-14, 15, and 16 did not inhibit the growth of mushroom Pleurotus eryngii ASI-2302, and Pleurotus ostreatus ASI-2042 and ASI-2180. When the culture broth mixture of the seven bacterial strains was applied to the mushroom media, the growths of pathogens, Pseudomonas sp. and Trichoderma sp., were inhibited.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.285-296
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• 1995

Anatomical studies of sink tissues are required for better understanding the biological plant growth system and energy metabolism. Kinematics of root growth zones of two genotypes of tall fescue (Festuca arundinacea Schreb.) receiving 50 or 200 ppm N were determined. Longitudinal anatomy and cell dynamics of root growth zones were studied and calculated. The root growth zone is organized similarly to the leaf growth zone which has cell division, elongation, and maturation zones, but the root growth zone is only about 3.0 mm long compared to 25 to 30 mm for the leaf growth zone. The root cap extends about 0.4 to 0.5 mm from the apical initial, while the cell elongation zone for both cortical and metaxylem cells extends about 3.3 mm from the apical initial for both genotypes and N levels. Root cap cells elongate from an initial length of about 5$\mu{m}$ long to a final length of about 40$\mu{m}$ before being sloughed. Initial lengths of cortical and metaxylem cells were about 8.5 $\mu{m}$ and 13.0 $\mu{m}$, respectively. Elongation of cortex and metaxylem cell showed sigmoidal curves with final lengths of about 120 $\mu{m}$ for cortex cells and 650 $\mu{m}$ for metaxylem cells. Initial size and final size for both types were not affected by N level, but cell fluxes and cell elongation rates of cortical and metaxylem cells were about double in low N. Cell production rates were about 5 to 6 times higher in cortical cells than in metaxylem cells. Differences in N caused a larger change in cell production rate, duration of cell elongation, and relative cell elongation rate than did the genotypes. These data indicate that N application affects root growth longitudinally by changing cell production rate and elongation rate.

• Korean Journal of Food Science and Technology
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• v.27 no.6
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• pp.1017-1027
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• 1995

This study was attempted to investigate physicochemical properties, molecular structural properties of native and acid-treated chestnut starch and chestnut starch gel. The amylose content was 18.9% and X-ray diffraction pattern showed Cb type. Swelling power was increased abruptly in the range of $65^{\circ}C{\sim}75^{\circ}C$ but increased slowly after that and solubility was increased abruptly until $70^{\circ}C$ but increased slowly after that. In amylograms which have different heating temperatures, cooling viscosity at $50^{\circ}C$ was reduced as heating temperature was increased. In molecular structural properties of amylose, ${\lambda}_{max}$ was 640 nm, ${\beta}-amylolysis$ limit was 84.2% and the degree of polymerization was 951 and in those of amylopectin, ${\lambda}_{max}$ was 570 nm, ${\beta}-amylolysis$ limit was 58.2%, the degree of polymerization was 1371 and average chain length was 22.6. In gel chromatography elution profiles of starch and amylose, 4.0% and 11.5% of low molecular weight-molecules($<5{\times}10^5$) were leached out. In gel chromatography elution profiles of soluble starch, the higher heating temperature was, the more high molecular weight-starches were leached out. The elution profiles after debranching amylopectin with pullulanase showed 2.2 of the ratio of peakIII(DP 10-15) to peakII(DP 35-45). Acid hydrolysis extent of 2.2 N HCI-treated starch at $35^{\circ}C$ for 10 days was 96% and hydrolysis rate showed two step pattern which had border line at 4 days. In elution profiles of acid treated chestnut starch, amylopectin peak was disappeared compeletly after 6 hrs and converted short chains of DP 10-15. Amylose content was increased until 6 hrs but decreased after that. Hardness of starch gel made at $75^{\circ}C$ of heating temperature and cohesiveness of starch gel made at $85^{\circ}C$ of heating temperature were the highest. Retrogradation rate of starch gels were relatively high, especially for the starch gel made at $75^{\circ}C$ of heating temperature.

• Korean Journal of Food Science and Technology
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• v.35 no.1
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• pp.138-143
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• 2003

Total methanolic extract of Chrysanthemum coronarium L. var. spatiosum (Compositae) was revealed to have anti-hepatotoxic activity against galactosamine-induced toxicity on primary cultured rat hepatocytes. After successive partitioning with chloroform, n-butanol, and water, the chloroform fraction showed a significant inhibition activity of 51% at 50 ppm, compared with that of silybin, 45.9% at $100\;{\mu}M$. The chloroform fraction was subjected to silica gel column chromatography and yielded active CH-II, CH-V and CH-VI subfractions, and the anti-hepatotoxic activity of these subfractions were 47.6, 56.3, and 23.2%, respectively, at 50 ppm. Total glutathione contents of CH-II, CH-V, and CH-VI increased by 49.8, 43.9, and 47.5% of the control, respectively at 50 ppm, whereas that of silymarin was, 59.7% at $100\;{\mu}M$ after challenged with galactosamine. The ratio of (reduced glutathione) / (total glutathione) in CH-II, CH-V and CH-VI subfraction showed similar values of $0.86{\sim}0.87$ at 50 ppm, whereas that of silymarin was, 0.85 at $100\;{\mu}M$. The incorporation of $[^3H]-uridine$ uptake into RNA was not affected by these active subfractions.