• Title/Summary/Keyword: 구리 chelation

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Copper Content Increase in E. coli Expressing Copper-Binding Peptide Genes (구리 결합 펩타이드의 발현에 의한 대장균 균체의 구리 함량 증가)

  • Kim, Hyung-Kee;Moon, Sung-Hyun;Kim, Woo-Yeon
    • Applied Biological Chemistry
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    • v.46 no.1
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    • pp.7-11
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    • 2003
  • Cloning and expression of copper-binding peptide gene in E. coli was carried out to enhance the copper-chelation capacity. E. coli was transformed with pET vector containing the copper-binding region of potato polyphenol oxidase gene and polyhistidine-coding DNA, and the copper content of E. coli harboring each vector was measured. No increase in intracellular copper was observed in E. coli harboring PPOCBpET32 vector, which contains DNA for polyphenol oxidase copper-binding region. Intracellular copper content of E. coli harboring pE728a vector, which contains one hexahistidine unit DNA, was 2,500 ppm after culturing without kanamycin, whereas E. coli harboring pET-his vector, which contains nine hexahistidine unit DNAs was 3,200 ppm.

Effects of Copper (II) Treatment in Soil on Tetracycline Toxicity to Growth of Lettuce (Lactuca sativa L.) (토양에서 상추의 생장에 대한 Tetracycline의 독성에 미치는 구리 (II)의 효과)

  • Lee, Byeongjoo;Min, Hyungi;Kim, Min-Suk;Kim, Jeong-Gyu
    • Ecology and Resilient Infrastructure
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    • v.4 no.1
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    • pp.63-70
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    • 2017
  • Tetracycline (TC) groups, widely used veterinary antibiotics, can enter into environment through animal manure application. TC forms a ligand complex with multivalent metal cations via chelation that can affect sorption and mobility of TC in soil. So far, however, it has been confirmed through the reaction of the soil outside in the aqueous solution and the evaluation of the performance in the soil cultivation process is insufficient. The purpose of this study was to examine effects of copper on TC toxicity to lettuce growth. In this research, $750mg\;kg^{-1}$ of TC and 2.5, 7.5, $17.5mg\;kg^{-1}$ of Cu are treated in soil and lettuce was cultivated in the treated soil. Growth difference of lettuce by treatment was observed. As a result, $750mg\;kg^{-1}$ of TC treated soil showed toxic effect to lettuce and the effect is alleviated by copper treatment.

Adsorption of copper ions from aqueous solution using surface modified pine bark media (표면개질된 소나무 수피를 이용한 수용액의 구리이온 흡착)

  • Park, Se-Keun;Kim, Yeong-Kwan
    • Journal of Korean Society of Water and Wastewater
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    • v.33 no.2
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    • pp.131-140
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    • 2019
  • This study used a packed column reactor and a horizontal flow mesh reactor to examine the removal of copper ions from aqueous solutions using pine bark, a natural adsorbent prepared from Korean red pine (Pinus densiflora). Both equilibrium and nonequilibrium adsorption experiments were conducted on copper ion concentrations of 10mg/L, and the removals of copper ions at equilibrium were close to 95%. Adsorption of copper ions could be well described by both the Langmuir and Freundlich adsorption isotherms. The bark was treated with nitric acid to enhance efficiency of copper removal, and sorption capacity was improved by about 48% at equilibrium; mechanisms such as ion exchange and chelation may have been involved in the sorption process. A pseudo second-order kinetic model described the kinetic behavior of the copper ion adsorption onto the bark. Regeneration with nitric acid resulted in extended use of spent bark in the packed column. The horizontal flow mesh reactor allowed approximately 80% removal efficiency, demonstrating its operational flexibility and the potential for its practical use as a bark filter reactor.

Interaction of Proline with Cu+ and Cu2+ Ions in the Gas Phase (기체상에서 Cu+ 및 Cu2+ 이온과 proline의 상호작용)

  • Lee, Gab-Yong
    • Journal of the Korean Chemical Society
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    • v.53 no.3
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    • pp.257-265
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    • 2009
  • The structures and metal affinities of the binding configurations of $Cu^{+}$ and $Cu^{2+}$ to proline have been investigated using the hybrid three-parameter Density Functional Theory(DFT/B3LYP). We found that the metal-proline bonding and the energy ordering of several conformers were very different in $Cu^{+}$-proline and $Cu^{2+}$-proline. For $Cu^{+}$-proline, the ground state structure was found to have a bidentated coordination in which $Cu^{+}$ was coordinated to the carbonyl oxygen and imino group nitrogen of neutral proline. On the contrary, the ground state structure of $Cu^{2+}$-proline involves chelation between the two oxygens of the carboxylate group in a zwitterionic proline. The metal ion affinity of proline of the most stable $Cu^{+}$-proline complex was calculated as 76.0 kcal/mol at 6-311++G(d,p) level, whereas the $Cu^{2+}$ ion affinity of proline was calculated as 258.5 kcal/mol.

Copper(II) Binding Mechanisms with Water Soluble Organic Fractions Extracted from Sewage Sludge Amended Soils (구리(II) 이온과 Sewage Sludge를 시용(施用)한 토양(土壤)에서 추출(抽出)한 수용성유기물(水溶性有機物)과의 착화합물(錯化合物) 형성방법(形成方法))

  • Lim, Hyung-Sik;Volk, V.V.;Baham, John
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.4
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    • pp.307-314
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    • 1986
  • Cu(II) binding mechanisms with water soluble organic fractions (WSOF) extracted from an agricultural soil (W), a soil treated with sludge for 6 years ($WS_6$), a sludge-soil mixture incubated for one week ($WS_1$), and sewage sludge (SS) were studied by electron spin resonance (ESR) spectroscopy and potentiometric titrations. Cu(II)-WSOF complexes produced $g_{11}$ values which were larger than $g_{\perp}$ values, indicating that the coordination of Cu(II) complex was an elongated octahedron. At liquid $N_2$ temperature (77K), the Cu(II)-W complex showed an anisotropic ESR spectrum while the Cu(II)-SS complex showed an isotropic spectrum. These spectral results suggest that the oxygen donor ligands of W may form relatively strong bonds with $Cu^{2+}$ due to extensive chelation while ligands of SS may form little or no chelate bonds with $Cu^{2+}$. The ESR spectra of Cu(II)-SS complex also suggest that each of four in-plane ligands (e.g., $COO^-$, $H_2O$, $Cl^-$, etc.) may act independently as monodentate ligands. Oxygen donor ligands such as aromatic carboxyl groups were probably the major Cu(II) binding sites in W. Sulfonate, aliphatic carboxyl group, and N-containing ligands were probably the major binding sites in SS at pH 5. The Cu(II) complexation with N-containing groups increased as sludge was added to the soil. Much higher (6x) pyridine concentrations were required to displace W from Cu(II)-W complex as compared to the Cu(II)-SS complex.

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