• Journal of Food Hygiene and Safety
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• v.36 no.4
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• pp.289-297
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• 2021

In this study, multiplex PCR and real-time PCR were performed on Theragra chalcogramma (walleye pollock), Pangasianodon hypophthalmus (iridescent shark) and their processed foods, such as changnan-jeot and gaiyang-jeot (salted iridescent shark intestine). Species-specific primers for T. chalcogramma and P. hypophthalmus were designed, and genomic DNA was directly extracted from each sample to perform single PCR and multiplex PCR. As a result of PCR, in the case of single PCR, PCR bands of T. chalcogramma (297 bp) and P. hypophthalmus (132 bp) were identified, and in the case of multiplex PCR, it was confirmed that amplification occurred without cross-reaction between T. chalcogramma and P. hypophthalmus. As a result of checking the PCR sensitivity, the concentration of genomic DNA was detected up to 0.1 ng/µL in both single PCR and multiplex PCR. The real-time PCR results showed that the average Ct value of T. chalcogramma was 20.765±0.691, and the average Ct value of P. hypophthalmus sample was 35.719±1.828 in the T. chalcogramma species-specific primers. In the P. hypophthalmus species-specific primers, the average Ct value of the T. chalcogramma sample was 35.996±1.423, and the mean Ct value of the P. hypophthalmus sample was 20.096±0.793. These results demonstrated the significant differences in the efficiency, specificity and cross-reactivity of species-specific primers in real-time PCR. Based on these findings, 7 of changnan-jeot or gaiyang-jeot products were confirmed by multiplex PCR and real-time PCR, and valid results were confirmed in all samples.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2009.05a
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• pp.182-183
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• 2009

플라즈마 원자층 증착법을 이용하여 Ru-TiN 빅막을 합성하였다. 박막 내 Ru의 함량은 Ru의 unit-cycle의 수에 따라 선형적으로 증가하였으며, Ru 함량이 증가함에 따라 박막의 비저항을 $3700{\mu}{\Omega}{\cdot}cm$에서 $190{\mu}{\Omega}{\cdot}cm$까지 자유롭게 조절할 수 있었다. Ru의 함량이 0.40 이상인 경우, Ru과 TiN 두물질이 교차 증착되어 서로의 결정 성장을 충분히 억제함으로서, 비정질구조를 가짐을 확인할 수 있었다. 또한, $O_2$ 분위기에서 열처리를 진행한 결과, Ru의 조성비가 0.40이상인 경우 $700^{\circ}C$까지 면저항의 변화가 거의 없음을 확인할 수 있었다.

• Journal of fish pathology
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• v.10 no.2
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• pp.75-86
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• 1997

Solid phase enzyme linked immunosorbent assay (ELISA) was developed to detect the whole cells of Edwardsiella tarda from infected tissues of flounder. Cross-reaction test was performed by ELISA against fish pathogens such as A. hydrophila ATCC7966. V. anguillarum HYUFP5001, Y, ruckeri 11-4, E. ictaluri and Streptococcus sp. NG8206. Rabbit anti-E, tarda Edk-2 sera highly cross-reacted with A. hydrophila ATCC7966 and V. anguillarum HUFP5001. However, the cross-reaction was removed by using the anti-serum pre-adsorbed with A, hydrophila ATCC7966 FKC. The intra-species cross-reaction among E. tarda isolates was very high. ELISA with the whole cell antigens present in tissue homogenate appeared with highly decreased sensitivity, presumably by the co-coating of lipid or proteins in tissues. Thus, it would be necessary to use the infected tissue homogenates diluted more than 100 times with PBS for diagnosis. Interestingly, compared with the using of FKC antigen, the direct detection of viable cells in tissue homogenate showed more sensitive results with detection limit of $1{\times}10^3$ cells/ml in buffer or diluted tissue homogenate. Consequently, the ELISA method developed in this study was specific, rapid and sensitive for diagnosing edwardsiellosis.

• Journal of the Society of Naval Architects of Korea
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• v.37 no.1
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• pp.67-81
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• 2000

An accurate and efficient numerical method for two-dimensional nonlinear radiation problem has been developed. The wave motion due to a moving body is described by the assumption of ideal fluid flow, and the governing Laplace equation can be effectively solved by the higher-order boundary element method with the help of the GMRES (Generalized Minimal RESidual) algorithm. The intersection or corner problem is resolved by utilizing the so-called discontinuous elements. The implicit trapezoidal rule is used in updating solutions at new time steps by considering stability and accuracy. Traveling waves caused by the oscillating body are absorbed downstream by the damping zone technique. It is demonstrated that the present method for time marching and radiation condition works efficiently for nonlinear radiation problem. To avoid the numerical instability enhanced by the local gathering of grid points, the regriding technique is employed so that all the grids on the free surface may be distributed with an equal distance. This makes it possible to reduce time interval and improve numerical stability. Special attention is paid to the local flow around the body during time integration. The nonlinear radiation force is calculated by the "acceleration potential technique". Present results show good agreement with other numerical computations and experiments.

• The Korean Journal of Applied Statistics
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• v.28 no.5
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• pp.1035-1046
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• 2015

Variance components models incorporate various random factors in the form of linear models. There are two experimental Layouts for the classification of factors under variance components models: nested classification and crossed classification. We consider two-way variance components models and investigate the effect of experimental Layout on the performance of model selection criteria AIC and BIC. The effect of experimental Layout is studied through a simulation study with various combinations of parameters in a systematic fashion. The simulation study shows differences in performance of model selection methods between the two classification. There is a particular tendency to prefer the smaller model than the true model when the variance component of a nested factor becomes relatively larger than a nesting factor that is persistent even when the sample size is not small.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.3
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• pp.342-351
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• 2016

This study was carried out to develop an optimized analytical method using high-performance liquid chromatography (HPLC) applied to canthaxanthin, which is not yet designated as a food colorant in Korea, as well as to perform validation and uncertainty evaluation of this method. Official methods of AOAC, UK, and Japan with HPLC-UV detection were evaluated for the analysis of canthaxanthin by comparison of linearity, resolution, selectivity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery, inter-laboratory tests, and uncertainty measurement. The calibration curves showed high linearity with an $R_2$ value of over 0.999 for canthaxanthin standard solutions in all three official methods. The official method of Japan exhibited the best results in terms of resolution and selectivity, including the lowest LOD and LOQ. The average coefficients of variation were calculated as less than five of three institutes with a precision value less than 1, accuracy near 100%, and recovery ratio between $100{\pm}10%$. The expanded uncertainty for canthaxanthin was estimated to be $39.5{\pm}5.29mg/kg$ (95% confidence level, k=2), and the uncertainty of measurement was 13.4%. In this study, official methods of canthaxanthin were compared and the validities verified. The results will be further applied to establish an authorized analytical method for canthaxanthin in Korea.

• Korean Journal of Food Science and Technology
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• v.44 no.5
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• pp.638-642
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• 2012

Human pathogenic viruses such as hepatitis A and E virus (HAV and HEV), which lead to acute liver failure and death, are foodborne pathogens associated with the consumption of virus-contaminated meats, filter-feeding bivalves, fruits, and salads. Two of the three swine farms examined in this study had HAV and HEV positive stool samples in a nested RT-PCR assay. The use of the immunomagnetic separation (IMS) facilitated the separation of HAV through interactions between the ligand on the virion surface and the antibody from the swine feces containing both HAV and HEV. The nested RT-PCR analysis was performed for the detection of HAV obtained from hepatocarcinoma cell line (PLC/PRF/5) contaminated with eluent fraction of IMS. This indicated that IMS has the potential to simultaneously isolate and concentrate target viruses by changing antibodies linked on the magnetic beads.

• Journal of Digital Contents Society
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• v.19 no.1
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• pp.141-147
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• 2018

This study analyzed the early retired employees who stayed in company no longer than 3 years based on a certain company's personality evaluation result data. The predicted model was analyzed by dividing into two categories; the manufacture group and the R&D group. Independent variables were selected according to the stepwise method. A logistic regression model was selected as a prediction model among various supervised learning methods, and trained through cross-validation to prevent over-fitting or under-fitting. The accuracy of the two groups were confirmed by the confusion matrix. The most influential factor for early retirement in the manufacture group was revealed as "immersion," and for the R&D group appeared as "antisocial." In the past, people concentrated on collecting data by questionnaire and identifying factors that are highly related to the retirement, but this study suggests a sustainable early retirement prediction model in the future by analyzing the tangible outcome of the recruitment process.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.226-226
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• 2017

최근 국지성 집중호우 및 돌발홍수와 같은 급격한 기상변화로 인한 기상재해의 발생빈도가 증가함에 따라 고해상도의 기상레이더 강수자료를 사용한 수공학 분야의 연구가 활발하게 진행되고 있다. 레이더 강수자료를 수문분석에 활용하는 목적은 레이더 강수량이 제공하는 공간분포를 최대한 활용하는데 있다. 기상레이더는 광범위한 영역에 대하여 시공간적으로 연속적인 관측이 가능하므로 지상 강수자료에 비하여 고해상도의 강수자료를 확보하는데 이점이 있다. 본 연구에서는 고해상도의 레이더 강수자료의 공간분포 특성을 유지하면서 지상 강수자료의 양적특성을 유지할 수 있는 조건부 합성기법을 개발하였다. 레이더 강수자료와 지상 강수자료를 조건부 합성하기 위하여 널리 활용되고 있는 Kriging, 역거리 가중법 및 Spline 보간법을 적용하였다. 조건부 합성결과는 지상 강수패턴을 현실성 있게 재현하였다. 추가적으로 미계측 지점으로 간주하여 보간법에 적용되지 않은 강수자료와 조건부 합성기법 결과에 대하여 교차검증을 수행한 결과 조건부 합성기법을 통한 강수정보는 수문분석에 직접적으로 활용될 수 있는 가능성을 확인하였다. 본 연구결과를 향후 초단기 레이더 강수예측기법과 연계하여 수문모형의 입력 자료로 활용한다면 보다 진보된 수문해석이 가능할 것으로 판단된다.

• Journal of Food Hygiene and Safety
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• v.32 no.1
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• pp.75-81
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• 2017

For people who have a food allergy the only way to manage the allergy is to avoid the food allergen. The mackerel is one of the major food allergens, but no immunoassay for the rapid and simple detection of mackerel has been reported. The objectives of this study are to develop and characterize monoclonal antibodies (MAbs) specific to mackerel using thermal stable-soluble proteins (TSSP) as an immunogen and to characterize the MAbs by indirect enzyme-linked immunosorbent assay (iELISA). The mice immunized with mackerel TSSP and showing high titer were used for cell fusion and cloning. The characterization of MAbs produced from hybridoma cells obtained was confirmed by indirect ELISA and western blot. Four MAbs were confirmed to be specific to mackerel without cross-reaction to other marine products and livestock products in the both methods. The iELISA and western blot based on the MAbs can sensitively detect 1% mackerel protein in other marine products. These results support that immunochemical methods based on the MAb produced could be used as rapid means to detect low levels of mackerel and to identify mackerel adulterated in food.