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http://dx.doi.org/10.3746/jkfn.2016.45.3.342

Validation and Uncertainty Evaluation of an Optimized Analytical Method Using HPLC Applied to Canthaxanthin, a Food Colorant  

Suh, Hee-Jae (Department of Food Science, Sun Moon University)
Kim, Kyung-Su (School of Food Science & Technology, Chung-Ang University)
Hong, Mi-Na (School of Food Science & Technology, Chung-Ang University)
Lee, Chan (School of Food Science & Technology, Chung-Ang University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.45, no.3, 2016 , pp. 342-351 More about this Journal
Abstract
This study was carried out to develop an optimized analytical method using high-performance liquid chromatography (HPLC) applied to canthaxanthin, which is not yet designated as a food colorant in Korea, as well as to perform validation and uncertainty evaluation of this method. Official methods of AOAC, UK, and Japan with HPLC-UV detection were evaluated for the analysis of canthaxanthin by comparison of linearity, resolution, selectivity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery, inter-laboratory tests, and uncertainty measurement. The calibration curves showed high linearity with an $R_2$ value of over 0.999 for canthaxanthin standard solutions in all three official methods. The official method of Japan exhibited the best results in terms of resolution and selectivity, including the lowest LOD and LOQ. The average coefficients of variation were calculated as less than five of three institutes with a precision value less than 1, accuracy near 100%, and recovery ratio between $100{\pm}10%$. The expanded uncertainty for canthaxanthin was estimated to be $39.5{\pm}5.29mg/kg$ (95% confidence level, k=2), and the uncertainty of measurement was 13.4%. In this study, official methods of canthaxanthin were compared and the validities verified. The results will be further applied to establish an authorized analytical method for canthaxanthin in Korea.
Keywords
canthaxanthin; food color; HPLC-UVD; uncertainty;
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  • Reference
1 Isler O. 1979. History and industrial application of carotenoids and vitamin A. Pure & Appl Chem 51: 447-462.   DOI
2 WHO. 1996. WHO Food Additives Series 35. World Health Organization, Geneva, Switzerland. Available from: http:// www.inchem.org/documents/jecfa/jecmono/v35je08.htm (assessed Jul 2015).
3 EFSA. 2010. Scientific opinion on the re-evaluation of canthaxanthin (E 161 g) as a food additive. EFSA Journal 8: 1852.   DOI
4 WHO. 1986. WHO Food Additives Series 22. World Health Organization, Geneva, Switzerland. Available from: http://www.inchem.org/documents/jecfa/jecmono/v22je09.htm (accessed Jul 2015).
5 FDA. 2015. Code of Federal Regulation Title 21. Food and Drug Administration, Washington, DC, USA. Available from: http://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/cfrs earch.cfm?fr=73.75 (accessed Jul 2015).
6 KFDA. 2010. Korea Food Additives Code. Korea Food and Drug Administration, Ohsong, Korea. Available from: http://www.mfds.go.kr/fa/%20%20index.do?nMenuCode=12& page_gubun=1&gongjeoncategory=1 (accessed Jul 2015).
7 FSA. 2010. Review and evaluation of available methods extraction and analysis for approved natural colours in food and drink. Food Standard Agency, London, UK. p 13-29.
8 AOAC. 2012. Official methods of analysis. 19th ed. Association of Official Analytical Chemists, Washington, DC, USA. Chapter 51, p 13.
9 JMHLW. 2009. Canthaxanthin analysis method. The Japan Ministry of Health, Labour and Welfare, Tokyo, Japan. Available from: http://www.mhlw.go.jp/topics/bukyoku/iyaku/syoku-anzen/zanryu3/2-042.html (accessed Jul 2015).
10 Ahuja S, Dong MW. 2005. Handbook of pharmaceutical analysis by HPLC. Elsevier Academic Press, London, UK. p 1-658.
11 EMEA. 2006. Validation of analytical procedures: Text and methodology. European Medicines Agency, London, UK. p 8-15.
12 ISO. 2004. Guide to the expression of uncertainty in measurement (GUM)-Supplement 1: Numerical methods for the propagation of distributions. International Organization for Standardization, Geneva, Switzerland. p 5-36.
13 CITAC. 2012. Quantifying uncertainty in analytical measurement. 3rd ed. Cooperation on International Traceability Analytical Chemistry, Sugiez, Switzerland. p 4-31.
14 Ellison SLR, Roesslein MI, Williams A. 2000. Quantifying uncertainty in analytical measurement. 2nd ed. EURACHEM, London, UK. p 32-94.
15 KRISS. 2010. Guide to the expression of uncertainty in measurement. Korea Research Institute of Standards and Science, Daejeon, Korea. p 1-142.
16 Kim KS. 2015. Comparison and optimization of the analytical method for canthaxanthin, a food additive. MS Thesis. Chung-Ang University, Gyeonggi, Korea. p 27-33.
17 BIPM. 2008. Evaluation of measurement data-Guide to the expression of uncertainty in measurement. Bureau International des Poids et Measures, Sevres, France. p 5-27.
18 EC. 2004. Report on the relationship between analytical results, measurement uncertainty, recovery factors and the provisions of EU food and feed legislation, with particular reference to community legislation concerning. European Commission, Brussel, Belgium. p 6-22.
19 Codex. 2004. Guidelines on measurement uncertainty CAC/GL 54-2004. Food and Agriculture Organization of the United Nations, Rome, Italy. p 5.