• Horticultural Science & Technology
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• v.34 no.5
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• pp.799-806
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• 2016

A standard rose cultivar, 'To Dios'(Rosa hybrida) was selected for use as a cut flower from the progeny of a cross between 'Vanilla perfume' and 'Marcia' at the research and development division of the Goyang-si Agricultural Technology Center in 2013. 'Vanilla perfume', an orange-colored (RHS Orange Group 27C) standard rose cultivar with 48 petals, was used as the female plant. 'Marcia', a white-colored (RHS Green white Group 157B) standard rose cultivar with 96 petals was used as the male parent. A cross was made in 2009 and seedlings were produced. Selections were made between 2010 and 2013, and a plant with good cut flower traits was finally selected and named 'To Dios'. 'To Dios' is a standard rose with large flowers of 11.2 cm in diameter and 128 peach-colored (RHS Red Group 36B) petals per flower. Vase life of this cultivar is up to 15 days. It takes 47 days from pruning to blooming and cut flower productivity is approximately $160stems/m^2$ per year. 'To Dios' was registered as a new cultivar No. 4875 with the Korea Seed & Variety Service on March 19, 2014.

• Journal of Plant Biotechnology
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• v.43 no.4
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• pp.466-472
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• 2016

This study analyzed the genetic diversity of 115 pear germplasms using 15 SSR markers. Three to forty-one SSR alleles were detected for each locus with an average of 16 alleles per locus. The average availability of markers was 0.966. The average observed heterozygosity ($H_{obs}$) was 0.603 (range: 0.140 to 0.929). The average expected heterozygosity ($H_{exp}$) was 0.718 (range: 0.463 to 0.904). The average polymorphism information content (PIC) was 0.692 (range: 0.403 to 0.897). The genetic relationships of pear germplasms were classified into two major groups by geographic origins and genetic characteristics according to genetic distance. The first group was composed of European pear belonging to Pyrus communis. The second group consisted of P. pyrifolia, P. ussuriensis, P. bretschneideri, P. betulaefolia, P. calleryana, interspecific hybrids, and unclear germplasms. The results of this study suggest that genotype analysis of pear germplasms using SSR markers can identify the genetic diversity of germplasms, and can be used to provide basic information for pear breeding.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.269-275
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• 2014

A standard rose cultivar, 'Love Letter' was selected for cut flower from the progenies of a cross between 'Red Giant' and 'Ensemble' by the rose breeding team of the Gyeonggi-do Agricultural Research & Extension Services (GARES) in 2011. A standard rose cultivar 'Red Giant' with red colored (RHS Red Group 45C) petals and 1.8 prickles per stems of 10 cm was used as a mother plant. A standard rose cultivar 'Ensemble' with white and red purple colored (RHS White Group 155C + RHS Red Group N57B) petals and 2.4 prickles per stems of 10 cm was used as a pollen parent. 'Love Letter' was crossed in 2007 and seedlings were produced. After tests of specific characters from 2008 to 2011, this cultivar was finally selected and named. As a standard type with large sized flower, it has red colored (RHS Red Group 46A) petals with 9.3 cm flower diameter and 32.4 petals per flower. Vase life of this cultivar could be as long as 12 days. It takes 43 days from pruning to blooming and cut flower productivity was 152 stems/$m^2$ in a year. The stems of cut flower have no thorn and the length is about with 70.5 cm. When this cultivar grew below $15^{\circ}C$ and 1,000 lux, the petals color became dark red in winter season. 'Love Letter' was registered as a new cultivar on Korea Seed & Variety Service (KSVS) with No. 4482 on May 8, 2013.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.51-51
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• 2018

본 연구는 바이오에너지용 이질3배체 억새(Miscanthus ${\times}$ giganteus) 품종육성 위한 교배재료인 물억새(M. sacchariflrous)와 참억새(M. sinensis) 출수기 단축과 단교배 방법을 개발하기 위해 수행하였다. 물억새와 참억새를 12시간 일장, 자연일장 조건에서 재배하여 출수기 단축에 미치는 단일효과를 조사하였다. 출수기에 일중 화분 발아시간, 절단한 화분친 이삭 활력 유지 및 격리방법 등 단교배 방법을 구명하여 이를 활용한 억새의 자가 수정 여부를 조사하고 시험교배를 실시하였다. 참억새와 물억새 모두 12시간 일장의 단일 조건에서 재배한 것이 자연일장에 재배한 것에 비해 출수 소요일수가 18~27일 정도 단축되었다. 화분 발아는 물억새에서 오전 6시에 왕성하였으나 시간이 경과할수록 발아율이 낮아져 오전 8시에는 10% 이하만 발아하였다. 참억새 화분은 오전 6시에는 발아하지 않았으나 오전 7시에 50% 이상이 발아하였고 8시에는 물억새와 같이 10% 이하였다. 화분친 참억새 이삭을 절단, 절화 보존액에 꽂아 백색 부직포로 격리하였을 때 절화 보존액량이 많을수록 활력 유지 일수가 증가하여 150 mL에서 물억새, 참억새 모두 7일간 개화 및 화분 비산을 지속하였다. 이 때 화분 발아율은 참억새와 물억새 모두 4일까지 40%를 유지하였다. 참억새와 물억새는 자가수정율이 ~ 2.5%로 낮고, 자연교잡 임실율은 출수기가 빠른 유전자원에서 54.4%까지 높았다. 상기 단교잡 방법을 적용한 물억새 4배체와 참억새 2배체간 14조합 시험교배로 437립의 종자를 얻었다. 본 연구의 억새 종간 단교배 방법은 우수한 종자친과 화분친으로 교배종자를 얻을 수 있어 향후 바이오매스 수량이 많으면서, 종자가 맺히지 않은 이질 3배체 품종육성에 활용할 수 있을 것으로 판단된다.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.715-718
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• 2010

$Cymbidium$ 'Purple Princess' ('Show Girl' x 'Eiko') is a new cultivar having dark purple sepal and petal (RHS, RP59A) with purple lip (RHS, RP59A). A cross was made between pink colored $C.$'Show Girl' as maternal line and dark purple colored $C.$ 'Eiko' as paternal line in 1994. It was preliminarily selected as Line No. 94019267 in 1999 considering the characters of flower shape and color, leaf growing shape and growth habit in normal culture condition. Multiplication, cultivation, and characteristic trials were conducted from 2000 to 2003. The line was named as Wongyo F1-7 and phenotype was characterized in 2004 as a new 'Purple Princess'. 'Purple Princess' has about 13.2 flowers per flower stalk and flower size of 5.2 cm. General appearance of petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming starts from early December under optimal culture condition. Leaf attitude and twisting is erect and very weak respectively. Erect leaf will be suitable for growing and handling in the nursery as pot orchid flowers.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.54-54
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• 2018

억새(Miscanthus spp.)는 우리나라 등 동아시아가 원산이며 바이오매스량이 많아 바이오에너지 원료작물로서 잠재성이 크기 때문에 2세대 바이오에탄올 생산 원료로 주목을 받고 있고, 축산깔개 및 토양개량제 등으로도 이용되고 있다. 미국과 유럽 등에서는 생태계 교란 방지를 위해 4배체 물억새(M. sacchariflorus)와 2배체 참억새(M. sinensis)의 종간 교잡 이질 3배체인 불임성 억새(M. x giganteous)을 주로 재배하고 있으나, 단일 유전형의 품종으로 병해충과 자연재해에 취약하여 다양한 억새 품종의 개발이 시급한 실정이다. 본 연구는 억새를 재료로 하여 반수체 및 배수체 확보를 통한 배수성 별 특성 평가와 함께 기내배양을 통해 탈분화 및 재분화 시스템을 구축하여 억새의 육종 효율을 높이기 위해서 실시하였다. 억새 종자로부터 캘러스의 유도는 MS배지와 N6배지에 1mg/L 2.4-D를 첨가하였을 때 비배발생 캘러스(nonembryogenic callus)가 유도되었고, N6배지에 3~5 mg/L 2,4-D를 첨가하였을 때 배발생 캘러스(embryogenic callus)가 발생하였다. MS배지보다는 N6배지에서 캘러스 유도율이 높았으며, 식물생장조절제로 2,4-D와 BA 조합처리 보다 2,4-D 단용 처리하였을 때 캘러스 유도율이 더 높았다. 억새 종에 따른 캘러스 유도율은 물억새가 25.2~49.3%, 참억새는 30.3~52.0%였고 거대억새는 62.6~81.1%로 나타났다. 억새 신초 및 줄기로부터의 캘러스 유도율은 물억새가 4.4~17.2%, 참억새는 1.8~7.7%, 거대억새는 15.3~19.9%로 나타나 종자에 비해 매우 낮았다. 미성숙화기로부터의 캘러스 유도는 억새 종에 따른 차이가 없었으며, 3mg/L 2,4-D 첨가 배지에서 캘러스 유도율이 비교적 낮았고(60~80%), 1mg/L와 5mg/L의 2,4-D가 첨가된 배지에서 캘러스 유도율이 높게(90~95%) 나타났다.

• Current Research on Agriculture and Life Sciences
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• v.20
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• pp.9-18
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• 2002

A total of 31 C. chinense lines selected from 2000 screening were tested again for resistance to P. capsid but resistance was not found in tile lines. A total of 26 selections from Korean land races tested 2001 spring were tested again for resistance to P. capsici, KC180, KC230, KC195 and KC194 showed moderate resistance to P. capsid. However, it was apparent on the basis of horticultural characteristics that KC180 and KC230 had been naturally crossed with AC2258 and CM334, respectively. CM334 and AC2248 seed lots that were increased in different years were taken out and tested for resistance to improve their genetic purity because the resistant genetic resources have been showing some off-types in tile population. Off-types began to be found in 1992 seed lots and tile proportion and degree of tile offs was increasing with time up to 2001. Plants true to the type in 1992 seed lots were selected and their inbred seeds were mass produced in a net cage in the greenhouse. AC2258 included in the experiment together was uniform. In 1995 seed lots of CM334, plants with resistance to P. capsici and low or no number of lateral branching at cotyledonary axil, although they were off from tile original CM334, were found and selection was applied to breed lines fixed in tile characters.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.622-630
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• 2017

The induction of regeneration and the maturation of the free-living gametophytes of Undaria pinnatifida were studied at temperatures(5, 10, 15 and $20^{\circ}C$), irradiance (5, 10, 20 and $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$) and photoperiods (14 : 10, 12 : 12 and 10 : 14 h L: D). Female gametophyte fragments were maintained in active regeneration without them reaching sexual maturity under conditions of $15^{\circ}C$, $20{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D); by contrast, the conditions for male gametophytes were slightly different at $15^{\circ}C$, $5{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 14 : 10 h (L: D). The sexual maturation of female and male gametophytes took place under $5^{\circ}C$, $20-40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D) and $15^{\circ}C$, $40{\mu}mol\;photons\;m^{-2}\;s^{-1}$, 10 : 14 h (L : D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for srain improvement as well as the cross breeding of U. pinnatifida.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.220-223
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• 2010

A FA intersectional hybrid lily cultivar 'Cherry Pink' was developed in 2006 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA) Korea. The cross and immature embryo rescue was conducted between female parents Lilium FA hybrid 'Supia' and male parents L. Asiatic hybrid 'A94-9' by cut style pollination method (CSM) at Suwon in 1999. The first selection was done and was tentatively named as 'FA01-17' in 2001. After in vitro multiplication, bulbing production of 'FA01-17' line, growth and flowering characteristic tests were conducted from 2002 to 2005. The evaluation of characteristics and consumer preferences were surveyed at a lily flower show of NIHHS at Taean in 2006. 'Cherry Pink' flowered in the middle of June and grew more than 120 cm stem in length. Flowers bloomed facing upward, a little spotted in petals and deep pink (RHS, RP27D). The pollen of 'Cherry Pink' was sterile. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. It was needed to control Botrytis disease in wet season.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.36-39
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• 2009

An Asiatic lily cultivar 'Apricot King' was bred in 2007 at National Horticultural Research Institute (NHRI), Rural Development Administration (RDA), Suwon, Korea. The cross was made in 2002 between Asiatic lily 'Orlando', a light orange colored cultivar, and 'Corrida', yellow and pink colored cultivar. Preliminarily selection was done as 'A04-72' in 2004. Multiplication and bulb formation, and characteristic tests were conducted from 2005 to 2006. The evaluation of this line was performed and named as 'Wongyo C1-84' in 2007 that was registered as 'Apricot King' to the registration office of Korea Seed & Variety Service. 'Apricot King' flowers at the end of June and grows average 121.6 cm. It flowers upward-facing, dark green throat and yellow orange (RHS 20A) with the size of flower 17.9 cm. Mean petal length and width is 12.8 cm and 3.7 cm, respectively. Leaves are 16.1 cm long and 2.1 cm wide. It shows light orange stigma and pollen. The weight and size of bulb is 64.2 g and 17.6 cm, respectively. For long-term storage, bulbs can be stored under -1 to $-2^{\circ}C$ for year-round forcing.