• Title/Summary/Keyword: 광 저해

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Effects of Low Dose γ-Radiation on Photosynthesis of Red Pepper (Capsicum annuum L.) and the Reduction of Photoinhibition (저선량 γ선 조사가 고추의 광합성과 광 스트레스 경감에 미치는 효과)

  • Lee, Hae-Youn;Baek, Myung-Hwa;Park, Soon-Chul;Park, Youn-Il;Kim, Jae-Sung
    • Korean Journal of Environmental Agriculture
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    • v.21 no.2
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    • pp.83-89
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    • 2002
  • The effect of low dose $\gamma$ radiation on photosynthesis and the reduction of photoinhibition in red pepper plant was investigated. The seedling height leaf width and leaf length of pepper were stimulated in plants grown from seeds irradiated with the low dose of 4 Gy. The $O_2$ evolution in the 4 Gy irradiation group was 1.5 times greater than in the control. To investigate the effect of low dose $\gamma$ radiation on response to high light stress, photoinhibition was induced in leaves of pepper by illumination of high light (900 $\mu mol/m^2/s$). Pmax was decreased with increasing illumination time by 20% in the control, while hardly decreased in the 4 Gy irradiation group. The photochemical yield of PSII, estimated as Fv/Fm, was decreased with increasing illumination time by 50% after 4 hours while Fo did not change. However, Fv/Fm in the 4 Gy irradiation group was decreased by 37% of inhibition, indicating that the photoinhibition was decreased by the low dose $\gamma$ radiation. Changes in the effective quantum yield of PSII, $\Phi_{PSII}$, and 1/Fo-1/Fm, a measure of the rate constant of excitation trapping by the PSII reaction center, showed similar pattern to Fv/Fm. And NPQ was decreased after photoinhibitory treatment showing no difference between the control and the 4 Gy irradiation group. These results showed the positive effect of low dose $\gamma$ radiation on the seedling growth and the reduction of photoinhibition.

광식성 누에품종 육성

  • 이상몽
    • Journal of Sericultural and Entomological Science
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    • v.34 no.2
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    • pp.59-66
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    • 1992
  • 1-4령 저염인공사료 공동육+5령 뽕잎육(농가개별육)$\longrightarrow$일주일 양잠에 적합한 광식성 누에품종육성에 필요한 기초지식을 얻기 위해 광식성유전자의 검색, 유전현상, 특성, 광식성계통 작출, 실용광식성 누에 품종육성방법 등에 대하여 소고하였다. 1. 광식성 유전자의 검색은 의잠을 사용하는 것이 가장 성력적이고 편리하며, 또한 섭식성을 정확히 파악할 수 있다. 2. 광식성 누에의 LP-1 사료에 대한 섭식성은 사육온도, 광조건, 발육단계 및 절식에 의하여 크게 영향 받지 않는다. 3. 광식성 유전자는 열성형질이며 1개의 주유전자와 수개의 수식유전자로 구성되어 있는 것으로 추찰된다. 4. LP-1 인공사료에 첨가한 morin 및 n-hexacosanol의 누에 섭식촉진효과는 미소하였으나, 상엽분말 첨가로 인한 누에 섭식촉진효과는 매우 양호하였다. 5. 광식성누에의 섭식성은 어분 및 효모에 의해 영향받지 않았다. 6. 섭식저해물질인 brucin 및 salicine은 정도의 차는 있으나 광식성누에의 섭식성을 저해하였다. 7. 광식성 및 비광광성누에의 LP-1 인공사료에 대한 섭식성의 차이는 누에의 두부에 있는 감각기관의 형태적 차이 때문이 아니라 기능적 차이에 기인하는 것으로 추찰된다. 8. 광식성 육성소재창출은 광식성유전자의 도입육종에 의해 가능하다. 9. 실용 광식성 누에품종육성을 위해 광식성검정에 적합한 인공사료를 사용하고, 실용형질이 우수하면서도 광식성유전자를 가진 계통을 선발, 교잡 또는 계통육성법에 의해 실용형질 및 섭식성을 개량하여 육종 목표를 달성하는 것이 최선책으로 생각된다.

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Cosmeceutical Activity of Broccoli (Brassica oleracea var. italica Plenck) with Different Light Sources (광원별 브로콜리 추출물의 화장품약리활성 검증)

  • Lee, Soo-Yeon;Jun, Hye-Ji;Yoon, Ji-Young;Kim, Tae-Su;Park, So-I;Lee, Sung-Pyo;Park, Joo-Hoon;Lee, Jin-Young
    • Journal of Life Science
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    • v.22 no.3
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    • pp.347-353
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    • 2012
  • The purpose of this study was to research the cosmeceutical activity of 70% ethanol extracts when Brassica oleracea var. italica Plenck (BO) was dried under red lighting (RLD), blue lighting (BLD), red+blue lighting (RBLD), or white lighting (WLD). The electron-donating abilities of 70% ethanol extracts from drying BO under RLD, BLD, RBLD, and WLD were 62.8%, 68.1%, 60.9%, and 69.9%, respectively, at a concentration of 500 ppm. The superoxide dismutase (SOD)-like activities of 70% ethanol extracts from drying BO under RLD, BLD, RBLD, and WLD were 39.2%, 47.2%, 19.6%, and 21.6%, respectively, at a concentration of 1,000 ppm. In terms of xanthine oxidase inhibition effects, the proportions of 70% ethanol extracts after drying BO under RLD, BLD, RBLD, and WLD were 45.1%, 56.2%, 38.8%, and 53.3%, respectively, at a concentration of 1,000 ppm. The tyrosinase inhibition effects of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 21.0%, 13.1%, 26.0%, and 19.1%, respectively, at a concentration of 1,000 ppm. The collagenase inhibition effect of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 47.4%, 20.8%, 54.6%, and 37.2%, respectively, at a concentration of 1,000 ppm. The astringent inhibition effects of 70% ethanol extracts when BO was dried under RLD, BLD, RBLD, and WLD were 21.5%, 39.6%, 40.0%, and 51.6%, respectively, at a concentration of 5,000 ppm. All of these findings suggest that BO extracts dried under RLD, BLD, RBLD, and WLD have great potential as a cosmeceutical ingredient with good biological activity.

Evaluation of Block-based Sharpening Algorithms for Fusion of Hyperion and ALI Imagery (Hyperion과 ALI 영상의 융합을 위한 블록 기반의 융합기법 평가)

  • Kim, Yeji;Choi, Jaewan
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.33 no.1
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    • pp.63-70
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    • 2015
  • An Image fusion, or Pansharpening is a methodology of increasing the spatial resolution of image with low-spatial resolution using high-spatial resolution images. In this paper, we have performed an image fusion of hyperspectral imagery by using panchromatic image with high-spatial resolution, multispectral and hyperspectral images with low-spatial resolution, which had been acquired by ALI and Hyperion of EO-1 satellite sensors. The study has been mainly focused on evaluating performance of fusion process following to the image fusion methodology of the block association, which had applied to ALI and Hyperion dataset by considering spectral characteristics between multispectral and hyperspectral images. The results from experiments have been identified that the proposed algorithm efficiently improved the spatial resolution and minimized spectral distortion comparing with results from a fusion of the only panchromatic and hyperspectral images and the existing block-based fusion method. Through the study in a proposed algorithm, we could concluded in that those applications of airborne hyperspectral sensors and various hyperspectral satellite sensors will be launched at future by enlarge its usages.

Effect of Light Conditions on Photoinhibition of Ginseng and Tobacco (광조건이 인삼과 잎담배의 광저해에 미치는 영향)

  • ;Il Hou
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.2
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    • pp.126-130
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    • 1985
  • Photoinhibition studies were conducted with ginseng (Panax ginseng) grown under shade and tobacco (N. tobaccum cv. Bulgaria) grown under full sunlight. The plants were exposed to light intensity of 580, 1280, 1770, 2580 ${\mu}$E/㎡/sec in normal air for 3.6 9 hours. Light saturation of ginseng was observed at 550 ${\mu}$E/㎡/sec and that of tobacco was at 1600 ${\mu}$E/㎡/sec. Tobacco exposed to high light intensity and long duration of light irradiation didn't show entire reduction in photosynthetic capacity. But in ginseng, 20.3% of photosynthesis was reduced in light intensity of 2,580 ${\mu}$E/㎡/sec during 9 hours. Light response in photosynthesis differed considerably between tobacco and ginseng, and ginseng exposed to high light intensity showed remarkable reduction in photosynthesis. The extent of photoinhibition of ginseng was dependent on the length of exposure to the high light intensity. Stomatal resistance of ginseng seemed not to be changed by photoinhibition but mesophyll resistance was increased.

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Kinetic Analysis of Cathepsin B Inhibitor Using a Spectrophotometric Assay (분광광도법에 의한 Cathepsin B 저해물질의 효소동력학적 저해특성 조사)

  • 한길환;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.2
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    • pp.90-95
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    • 2001
  • Kinetic Analysis of Cathepsin B Inhibitor Using a Spectrophotometric Assay. Han, Kil-Hwan and SangDal Kim*. Department of Applied MicrobioJ0f5Yt Yeungnam UniversitYt Kyongsan 77 2-749, Korea - The KHS 10, C4Hl10~6 formula produced from Streptomyces luteogriseus KT-] 0 effectively inhibited a lysosomal cysteine proteinase, cathepsin B. It inhibited the enzyme activity of cathepsin B competitively when the N a-CBZ-Llysine p-nitrophenyl ester HC] (CLN) was used as a substrate. The inhibition const:mt (Ki) of KHS 1 0 for cathepsin B detennined by spectrophotometeric assay was 430 nM. The effective inhibition of cathepsin B was observed at $25^{\circ}C$ :md pH 6.0. The cathepsin B inhibitor, KHSlO needed a preincubation of cathepsin B with the inhibitor for over 5 min. The KHS 10 preserved over 80% inhibition activity even after heat-treatment at $100^{\circ}C$ for ] hr.

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Development of Prototype Biosensor for The Detection of Organophosporus Compounds (유기인화합물 측정용 광바이오센서 개발)

  • 최정우;김종민;이원홍;김영기
    • KSBB Journal
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    • v.17 no.2
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    • pp.158-161
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    • 2002
  • In this study, a prototype fiber-optic biosensor was fabricated using the inhibition of enzyme reaction by organophosphorus compounds to detect organophosphorus compounds, which is nervous toxic material an? is used as chemical weapon and pesticide. Enzyme, substrate, and inhibitor for enzyme reaction were acetylcholinesterase (key enzyme in nervous cell), acetylthiocholine iodide, and paraoxon (a kind of organophosphorus compounds), respectively. The detection principle of sensor is the detection of enzyme reaction inhibited by organophosphorus compounds by the quantitative measurement of acetic acid, which was achieved by absorbance measurement using litmus solution that maximum absorbance band is changed by pH. To fabricate prototype fiber-optic biosensor, high bright LED and photodiode was used as light source and light intensity detector, respectively. From the experimental results using a prototype biosensor, the linear change of sensor signal was obtained in a range of 0-2 ppm inhibitor concentrations. From these results, it was verified that the quantitative measurement of organophosphorus compounds could be achieved fast (within 2 minutes) and accurately by a prototype fiber-optic biosensor.

High Resolution Reconstruction of EO-1 Hyperion Hyperspectral Images Using IKONOS Images (IKONOS 영상을 이용한 EO-1 Hyperion Hyperspectral 영상자료의 고해상도 구축)

  • Lee, Sang-Hoon
    • Korean Journal of Remote Sensing
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    • v.24 no.6
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    • pp.631-639
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    • 2008
  • This study presents an approach to synthesize hyperspectral images of lower resolution at a higher resolution using the high resolution images acquired from a sensor of commercial satellites. The proposed method was applied to the reconstruction of EO-1 Hyperion images using the images acquired from IKONOS sensor. Based on the FitPAN-Mod pansharpening technique (Lee, 2008b), the hyperspectral images of 30m resolution were reconstructed at 1m resolution of IKONOS panchromatic image. In this study, the synthesized hyperspectral images of 50 bands, whose wavelengths range in the wavelength of panchromatic sensor, were generated from the three stages of high resolution reconstruction using FitPAN-Mod. The experimental results show that the proposed method effectively integrates the spatial detail of the panchromatic modality as well as the spectral detail of the hyperspectral one into the synthesized image. It indicates the proposed method has a potential as a technique to produce alternative images for the images that would have been observed from a hyperspectral sensor at the high resolution of commercial satellite images.

Blue Light Photosensitization in Mitochondrial Membrane of Plant Cells (식물세포 미토콘드리아막에서 일어나는 청색광 Photosensitization)

  • Kim, Kyung-Hyun;Kim, Jong-Pyung;Jung, Jin
    • Korean Journal of Environmental Agriculture
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    • v.6 no.2
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    • pp.94-100
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    • 1987
  • Plant mitochondria, irradiated with blue-colored $sunlight(350{\sim}500nm)$ under aerobic and anaerobic conditions, were assayed as to the electron transfer activity of respiratory enzyme system, and compared with those irradiated with orange-colored light(white sunlight minus blue-colored light). The respiratory activity of mitochondria was most seriousely inhibited by illumination with blue-colored light under aerobic condition. Deaeration of mitochondrial suspension resulted in substantial decrease of the photoinhibition by blue-colored light. Meanwhile, orange-colored light demonstrated much less effectiveness-almost ineffectiveness-in causing the inhibition of mitochondrial respiration system. The results of enzymatic assay revealed a strong possibility that FMN in NDH and heme group at least in cytochrome c oxidase, but not FAD in SDH, are the photodynamic sensitizers in mitochondrial inner membrane. Also worthwhile to note is the significant difference from the others of SDH in its photoinhibitory response to the light quality of visible light; that the inhibition of SDH by irradiation was not affected by atmospheric condition and that orange-colored light gave rise to considerable extents of inhibition to the enzyme. This observation was tentatively interpreted in terms of photosensitized reaction not involving molecular oxygen possibly catalyzed by Fe-S centers in the enzyme. The superoxide production and the membrane peroxidation of mitochondria under various treatments also indicated that there was blue-light photodynamic reaction in mitochondria involving active oxygens.

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