• Transactions of the Korean hydrogen and new energy society
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• v.22 no.4
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• pp.451-457
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• 2011

Purple non-sulfur (PNS) bacterium $Rhodobacter$ $sphaeroides$ KD131 was studied with the aim of achieving maximum hydrogen production using various carbon and nitrogen sources at different pH conditions. Cells grew well and produced hydrogen using $(NH_4){_2}SO_4$ or glutamate as a nitrogen source in combination with a carbon substrate, succinate or malate. During 48h of photo-heterotrophic fermentation under 110$W/m^2$ illumination using a halogen lamp at $30^{\circ}C$, 67% of 30mM succinate added was degraded and the hydrogen yield was estimated as 3.29mol $H^2$/mol-succinate. However, less than 30% of formate was consumed and hydrogen was not produced due to a lack of genes coding for the formate-hydrogen lyase complex of strain KD131. Initial cell concentrations of more than 0.6g dry cell weight/L-culture broth were not favorable for hydrogen evolution by cell aggregation, thus leading to substrate and light unavailability. In a modified Sistrom's medium containing 30mM succinate with a carbon to nitrogen ratio of 12.85 (w/w), glutamate produced 1.40-fold more hydrogen compared to ammonium sulfate during the first 48h. However, ammonium sulfate was 1.78-fold more effective for extended cultivation of 96h. An initial pH range from 6.0 to 9.0 influenced cell growth and hydrogen production, and maintenance of pH 7.5 during photofermentation led to the increased hydrogen yield.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.147-148
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• 2003

최근 새로운 바다 자원으로서 “해양 심층수”가 주목받고 있다. 해양 심층수(深層水, deep sea water)는 태양광이 도달하지 않는 수심 200 m 이상 깊이의 물로 수온이 2$^{\circ}C$ 이하로 연중 거의 변화가 없는 저온안정성과 무기 영양염을 다량 포함하는 특징이 있어 심층수의 활용도는 매우 높다. NaCl만 제거하면 훌릉한 생수가 되기 때문에 심층수를 원료로 한 식품, 화장품, 의약품 개발에 대한 관심이 높아지고 있다. 미국, 일본 등지에서는 10여년전부터 심층수를 이용한 에너지, 수자원, 생수 및 식품 산업이 활발히 진행되어졌다. 현재 식품 산업분야로는 두부제조, 빵, 청주, 간장, 된장 등의 발효식품에 대한 연구가 진행되어지고 있다. 심층수를 적당하게 첨가하면 알코올 농도가 높아지는 발효강화작용이 있다. 새로운 건강 먹거리를 갈망하는 현대인들에게 해양 심층수를 이용한 발효식품은 매우 환영받을 것으로 사료된다. 이에 본 연구는 해양 심층수의 다양한 자원성을 효율적으로 활용하기 위해 심층수의 청정성과 풍부한 미네랄을 함유한 심층수의 농도에 따른 효모의 증식도를 알아봄으로서 적응력 강한 효모를 조사하였다. 실험에 사용한 해양 심층수는 淸凉飮料水(日本ナチュラルヘルス株式會社, 深透水 1550) 사용하였다. 효모균주는 경북과학대 전통식품 연구소에서 보유하고 있는 9종의 효모를 사용하였으며, 심층수 경도 250, 500, 1000의 심층수와 1차 증류수(대조군)에 sucrose 10% 첨가한 당용액을 사용하였다. 또한 당의 적응력을 알아보기 위해 sucrose 10, 15, 20% 첨가 당용액을 사용하여 Sacch.cerevisiae kluyvery DJ97 효모의 증식도를 알아보았다. 그 결과, Saccharomyces cerevisiae 12호 균주가 심층수 경도 500인 시험군에서 증식력이 높았으며, Saccharomyces cerevisiae 901 균주는 경도 1000인 시험군이 대조군에 비해 증식력이 높게 나타나 효모균주의 종류와 심층수의 첨가량에 따라 적응력이 차이가 있었다. 당농도와 심층수 첨가량에 따른 효모 균주의 발효력을 알아본 결과 심층수 경도 200의 당 10% 첨가군에서 가장 잘 증식하는 것으로 나타나, 알코을 효모균주의 발효력을 높이는데는 최적의 농도가 있음을 알 수 있었다. 따라서 심층수를 이용한 다양한 발효식품에 대한 연구가 요구되어진다.

• The Journal of the Convergence on Culture Technology
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• v.6 no.2
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• pp.567-572
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• 2020

The purpose of this study was to investigate the effects of Anti-Thrombotic Activities and Cardiovascular Improvement of Fermented Garlic Extracts. The incidence of cardiovascular diseases (CVDs) is increasing rapidly in developed countries, with CVDs now representing the leading cause of morbidity and mortality. Natural products and ethnomedicines have been shown to reduce the risk of CVDs. Garlic is a medicinal plant used throughout the world for its anti-inflammatory, antioxidant, and antiplatelet activities. We hypothesized that fermented preparations of these products may possess stronger antiplatelet effects than the non-fermented forms owing to the increased bioavailability of the bioactive compounds produced during fermentation. Therefore, we compared these compounds via in vitro and ex vivo platelet aggregation assays by using standard light transmission aggregometry and ex vivo granule secretions from rat platelets. We found that fermented preparations exerted more potent and significant inhibition of platelet aggregation both in vitro and ex vivo. Likewise, ATP release from dense granules of platelets was also significantly inhibited in fermented preparation-treated rat platelets compared to that in non-fermented preparation-treated ones. We concluded that fermented preparations exerted more potent effects on platelet function both in vitro and ex vivo, possibly as a result of the increased bioavailability of active compounds produced during fermentation. We therefore suggest that fermented products may be potent therapeutics against platelet-related CVDs and can be used as antiplatelet and antithrombotic agents.

• Korean Journal of Food Science and Technology
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• v.48 no.1
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• pp.48-53
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• 2016

The objective of this study was to evaluate the accuracy and efficiency of different methods used for the fermentable sugar assay in the production of Makgeolli sul-dut. In the initial stage of fermentation, Ipguk treatment produced a higher alcohol content compared to the Nuruk treatment. However, the alcohol content was not significantly different between the two starters at the final stage of fermentation. Acidity in the Ipguk treatment was higher than that of Nuruk throughout the fermentation period. After analyzing the fermentable sugars using dinitrosalicylic acid (DNS), Fehling's method, refractometer, glucose kit, and high performance liquid chromatography (HPLC), it was confirmed that the HPLC method was the most accurate for fermentable sugar quantification. In both types of starters, DNS and Fehling's methods showed results comparable to HPLC in terms of fermentable sugar content, while the glucose kit and refractometer analyses showed relatively large discrepancies, indicating that the Fehling's method could also be effective for the analysis of fermentable sugars in the manufacture of Makgeolli.

• KSBB Journal
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• v.5 no.1
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• pp.37-42
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• 1990

In on-line measurement, there were two difficult problems to remove the biomass in the sample and to prevent the contamination at the sampling. In this study, the problem of the contamination at the sampling was solved by using the sampling device consisting of sterilizable sampling line, peristaltic pump and three-way valve. The glucose concentration was measured by glucose calibration equation without removing the biomass. Xanthan fermentation was performed satisfactorily using this automatic analyzing device.

• Journal of Bio-Environment Control
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• v.12 no.1
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• pp.17-21
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• 2003

This experiment was conducted to find out the method of preventing decrease in the marketable yield of oriental melon (Cucumis melo L. var. makuwa MAKINO) under low light intensity. By maintaining low light of 400 $\mu$mol$.$m$^{[-10]}$ 2$.$S$^{-1}$ from 10 days after fruit set to fruit enlargement period, the photosynthetic rate and chlorophyll contents of leaf were reduced. Leaves which had no urea application showed largely decreased photosynthetic rate The content of soluble solids was lower$.$ in the low light than natural light treatment. Regardless of foliar application of urea, % fermentation fruits was under 4% in the natural light treatment and over 10% in the low light treatment. The less the fruit thinning, the greater was % fermentation fruits under low light condition. The % fermentation fruits were 39% and 40% in no fruit thinning treatment. The harvest was delayed under low light condition regardless of foliar fertilization. As the number of thinned fruits was decreased, the harvest time was delayed more. Marketable yield per plant sharply decreased under low light intensity. Compared with natural light, the yield under low light treatment was 16∼34%. The treatment fertilized with 0.5% urea on leaf had 34% greater harvest index of marketable yield than other treatments. In conclusion, when the long low light condition from 10th day after fruiting was forecasted, thinning two fruits out of six fruits and two times foliar fertilization with 0.5% urea should be applied.

• Proceedings of the KIEE Conference
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• 2005.07b
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• pp.1693-1695
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• 2005

최근 공공건물에 신재생에너지 적용이 의무화되었고, 기후변화협약의 발효로 이산화탄소배출 절감에 대한 관심이 매우 높다. 가장 효율이 높은 냉난방 시스템인 지열히트펌프를 대표적인 신재생에너지인 태양광발전을 이용하여 구동하여 건물을 냉난방을 수행하도록 적용되었다. 야간 또는 태양에너지 밀도가 낮은 날을 위하여 축열과 축냉을 할 수 있는 수축열시스템과 연계하여 적용되었다. 지열히트펌프는 도심지역의 열섬현상 해소에 기여할 수 있으며, 외기 온도의 변화에 관계없이 쾌적한 실내환경을 유지할 수 있으며 향후에 그린 빌딩인증등을 위해 에너지 절약과 신재생에너지 사용량을 높일 수 있는 방안으로 판단된다. 이를 적용한 설치 사례에 대한 사양과 운전 조건이 제시된다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.3
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• pp.369-375
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• 2010

To evaluate the skin aging inhibition effect of fermented Reynoutria elliptica, skin aging model was produced by the irradiation of UVB to hairless mice for 5 weeks. The skin erythema index for the positive control (PC), not fermented Reynoutria elliptica extract (NFR), and fermented Reynoutria elliptica extract (FR-500, 1000, 2000) groups were lower than that of the control group. However, both lipid and water capacity for the PC and FR groups were higher than those of the control group. Collagen fibers in dermis of the FR groups were almost intact with a regular arrangement which were similar to the normal (NO) group. Also, relatively much less number of mast cells and inflammatory cells were found in FR groups. The skin TBARS contents and XO activity in the FR group were significantly lower than the control group. The activities of GSH, SOD and CAT for the FR groups were significant higher than the control group. Therefore, fermented Reynoutria elliptica extract can be practically useful for the prevention or improvement of skin aging in terms of health promotion and beauty for the people.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.554-562
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• 2021

Lactobacillus plantarum SM4, a strain producing β-glucosidase, was isolated from kimchi and fermented with white Taraxacum coreanum to enhance the production of bioactive compounds. The total polyphenol content(TPC), total flavonoid content(TFC), radical scavenging activity(RSA), tyrosinase inhibitory activities(TIA), and collagenase inhibitory activities(CIA) were measured to evaluate the skin whitening and anti-wrinkle effects of the fermented product. The TPC of fermented white T. coreanum was 41.8±0.26 mg GAE/g DW, which was approximately two times higher than the hot-water extraction of 21.4±0.67 mg GAE/g DW. RSA, an indicator of antioxidant activity, was 65.6±4.7% in fermentation, which is four times higher than that of the hot-water extract. TAI and CAI, which are indicators of the whitening and anti-wrinkle effects, were 87.9±4.73% and 66.7±3.48%, respectively, which were 2.4 and 1.5 times higher than those of hot-water extraction. When comparing the UVA(320 nm) protection effects of fermented and hot-water extraction, the fermented white T. coreanum showed higher protection with an absorption rate of 64.7% and 15.2%, respectively. The white T. coreanum fermented product showed higher bioactive properties and improved skin whitening, anti-wrinkle, and UV protection effects through the production of β-glucosidase from L. plantarum SM4.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.1
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• pp.47-55
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• 2009

In this study, the antioxidative effects, inhibitory effects on tyrosinase, and component analysis of fermented Melissa officinalis extracts were investigated. The ethyl acetate fraction of fermented extract ($8.38{\mu}g/mL$) showed the most prominent the free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activities ($FSC_{50}$) of extract/fractions of M. officinalis. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of some M. officinalis extracts on ROS generated in $Fe^{3+}$-EDTA/$H_{2}O_{2}$ system were investigated using the luminol-dependent chemiluminescence assay. The ethyl acetate fraction of fermented extract ($0.63{\mu}g/mL$) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of M. officinalis on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The M. officinalis extracts suppressed photohemolysis in a concentration dependent manner ($5\;{\sim}\;75{\mu}g/mL$). The inhibitory effect of M. officinalis extracts on tyrosinase was investigated to assess their whitening efficacy. Inhibitory effects ($IC_{50}$) on tyrosinase of some M. officinalis extracts was 50 % ethanol extract ($365{\mu}g/mL$) < ethyl acetate fraction of fermented extract ($122.43{\mu}g/mL$) < ethylacetate fraction ($94.8{\mu}g/mL$). Fractions of ethyl acetate both from ordinary and fermented M. officinalis extracts showed 2 band in TLC and 2 peak in HPLC (330 nm). In HPLC chromatogram of ethyl acetate fraction, peak 1 (51.64 %) and peak 2 (48.36 %) were identified as caffeic acid and rosmarinic acid in the order of elution time. Also, in HPLC chromatogram of ethyl acetate fraction of fermented extract, peak 1 (4.13 %) and peak 2 (95.87 %) were identified as caffeic acid and rosmarinic acid in the order of elution time. These results indicate that the component and content of ordinary and fermented extracts of M. officinalis are different. And the extract of M. officinalis can be used as an antioxidant.