Transactions of the Korean hydrogen and new energy society (한국수소및신에너지학회논문집)

The Korean Hydrogen and New Energy Society (한국수소및신에너지학회)
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Photo-Fermentative Hydrogen Production by Rhodobacter Sphaeroides KD131 under Various Culture Conditions

다양한 배양조건에 따른 Rhodobacter sphaeroides KD131의 광발효 수소생산

  • Son, Han-Na (Wastes Energy Research Center, Korea Institute of Energy Research) ;
  • Kim, Dong-Hoon (Wastes Energy Research Center, Korea Institute of Energy Research) ;
  • Lee, Won-Tae (Construction and Environment Research Division, Korea Institute of Construction Technology) ;
  • Rhee, Young-Ha (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Kim, Mi-Sun (Wastes Energy Research Center, Korea Institute of Energy Research)
  • 손한나 (한국에너지기술연구원 폐자원연구센터) ;
  • 김동훈 (한국에너지기술연구원 폐자원연구센터) ;
  • 이원태 (한국건설기술연구원 건설환경연구실) ;
  • 이영하 (충남대학교 분자미생물학 및 생명공학과) ;
  • 김미선 (한국에너지기술연구원 폐자원연구센터)
  • Received : 2011.05.31
  • Accepted : 2011.08.01
  • Published : 2011.08.30
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Abstract

Purple non-sulfur (PNS) bacterium $Rhodobacter$ $sphaeroides$ KD131 was studied with the aim of achieving maximum hydrogen production using various carbon and nitrogen sources at different pH conditions. Cells grew well and produced hydrogen using $(NH_4){_2}SO_4$ or glutamate as a nitrogen source in combination with a carbon substrate, succinate or malate. During 48h of photo-heterotrophic fermentation under 110$W/m^2$ illumination using a halogen lamp at $30^{\circ}C$, 67% of 30mM succinate added was degraded and the hydrogen yield was estimated as 3.29mol $H^2$/mol-succinate. However, less than 30% of formate was consumed and hydrogen was not produced due to a lack of genes coding for the formate-hydrogen lyase complex of strain KD131. Initial cell concentrations of more than 0.6g dry cell weight/L-culture broth were not favorable for hydrogen evolution by cell aggregation, thus leading to substrate and light unavailability. In a modified Sistrom's medium containing 30mM succinate with a carbon to nitrogen ratio of 12.85 (w/w), glutamate produced 1.40-fold more hydrogen compared to ammonium sulfate during the first 48h. However, ammonium sulfate was 1.78-fold more effective for extended cultivation of 96h. An initial pH range from 6.0 to 9.0 influenced cell growth and hydrogen production, and maintenance of pH 7.5 during photofermentation led to the increased hydrogen yield.

Keywords

References

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