Browse > Article
http://dx.doi.org/10.7316/khnes.2011.22.4.451

Photo-Fermentative Hydrogen Production by Rhodobacter Sphaeroides KD131 under Various Culture Conditions  

Son, Han-Na (Wastes Energy Research Center, Korea Institute of Energy Research)
Kim, Dong-Hoon (Wastes Energy Research Center, Korea Institute of Energy Research)
Lee, Won-Tae (Construction and Environment Research Division, Korea Institute of Construction Technology)
Rhee, Young-Ha (Department of Microbiology and Molecular Biology, Chungnam National University)
Kim, Mi-Sun (Wastes Energy Research Center, Korea Institute of Energy Research)
Publication Information
Journal of Hydrogen and New Energy / v.22, no.4, 2011 , pp. 451-457 More about this Journal
Abstract
Purple non-sulfur (PNS) bacterium $Rhodobacter$ $sphaeroides$ KD131 was studied with the aim of achieving maximum hydrogen production using various carbon and nitrogen sources at different pH conditions. Cells grew well and produced hydrogen using $(NH_4){_2}SO_4$ or glutamate as a nitrogen source in combination with a carbon substrate, succinate or malate. During 48h of photo-heterotrophic fermentation under 110$W/m^2$ illumination using a halogen lamp at $30^{\circ}C$, 67% of 30mM succinate added was degraded and the hydrogen yield was estimated as 3.29mol $H^2$/mol-succinate. However, less than 30% of formate was consumed and hydrogen was not produced due to a lack of genes coding for the formate-hydrogen lyase complex of strain KD131. Initial cell concentrations of more than 0.6g dry cell weight/L-culture broth were not favorable for hydrogen evolution by cell aggregation, thus leading to substrate and light unavailability. In a modified Sistrom's medium containing 30mM succinate with a carbon to nitrogen ratio of 12.85 (w/w), glutamate produced 1.40-fold more hydrogen compared to ammonium sulfate during the first 48h. However, ammonium sulfate was 1.78-fold more effective for extended cultivation of 96h. An initial pH range from 6.0 to 9.0 influenced cell growth and hydrogen production, and maintenance of pH 7.5 during photofermentation led to the increased hydrogen yield.
Keywords
Biohydrogen; Rhodobacter sphaeroides KD131; Photo-fermentation; PNS bacteria;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Shi X.-Y., Yu H-Q. "Optimization of glutamate concentration and pH for $H_{2}$ production from using spent media of Enterobacter cloacae DM11. Appl Microbiol Biotechnol, Vol. 68, No. 4, 2005, pp. 533-541.   DOI   ScienceOn
2 SasikaLa K., Ramana C. V., Rao P. R. "Environmental regulation for optimal biomass yield and photoproduction of hydrogen by Rhodobacter sphaeroides O.U.001" Int J Hydrogen Energy, Vol. 16, No. 9, 1991, pp. 597-601.   DOI   ScienceOn
3 Argun H., Kargi F., Kapdan I. K. "Effects of the substrate and cell concentration on biohydrogen production from ground wheat by combined dark and photo fermentation" Int J Hydrogen Energy, Vol. 34, No. 15, 2009, pp. 6181-6188.   DOI   ScienceOn
4 손한나, 김미선 "Rhdobacter sphaeroides KD131에 의한 유기산 광합성 발효 최적화" 한국수소 및 신에너지학회 논문집, 21권, 2호, 2010, pp. 136-142.
5 Waligorska M, Seifert K, Gorecki K, Moritz M, Laniecki M, "Kinetic model of hydrogen generation by Rhodobacter sphaeroides in the presence of $NH_{4}^{+}$ ions" J Appl Microbiol, Vol. 107, 2009, pp. 1308-1318.   DOI   ScienceOn
6 Cselovszky J, Wolf G, Hammes WP, "Production of formate, acetate and succinate by anaerobic fermentation of Lactobacillus pentosus in the presence of citrate" Appl Microbial Biotechnol Vol. 37, 1992, pp. 94-97.
7 Gunduz U., Turkarslan S.,Yucel M., Turker L., Eroglu I. "Hydrogen production by different strains of R. sphaeroides." Hydrogen energy progress XI. Proceedings of the 13th WHEC, Beijing- China, Vol. 1, No. 2000, pp. 434-439.
8 Zabut B., EL-KahLout K., Yucel M., Gunduz U., Turker L., ErogLu I. "Hydrogen gas production by combined systems of Rhodobacter sphaeroides O.U.001 and Halobacterium salinarum in a photo-bioreactor" Int J Hydrogen Energy, Vol. 31, No. 1, 2006, pp. 1553-1562.   DOI   ScienceOn
9 Kim M. S, Baek J. S, Lee J. K. "Comparison of $H_{2}$ accumulation by Rhodobacter sphaeroides KD131 and its uptake hydrogenase and PHB synthase deficient mutant" Int J Hydrogen Energ, Vol. 31, No. 1, 2006, pp. 121-127.   DOI   ScienceOn
10 Koku H., Eroglu I., Gündüz U., Yücel M., Türker L. Aspects of the metabolism of hydrogen production by Rhodobacter sphaeroides. Int J Hydrogen Energy, Vol. 27, No. 11-12, 2002, pp. 1315-1329.   DOI   ScienceOn
11 Lee I. H., Park J. Y., Kho D. H., Kim M. S., Lee J. K. "Reductive effect of $H_{2}$ uptake and polybeta- hydroxybutyrate formation on nitrogenasemediated $H_{2}$ accumulation of Rhodobacter sphaeroides according to Light intensity" Appl Microbiol Biotechnol, Vol. 60, No. 1-2, 2002, pp. 147-153.   DOI
12 Sistrom W. R. "A requirement for sodium in the growth of Rhodopseudomonas spheroides" J Gen Microbiol, Vol. 22, No. 3, 1960, pp. 778-785.   DOI   ScienceOn
13 Redwood M. D, Paterson-BeedLe M., Macaskie L. E. "Integrating dark and Light biohydrogen production strategies: towards the hydrogen economy" Rev Environ Sci Biotechnol, Vol. 8, No. 2, 2009, pp. 149-185.   DOI   ScienceOn