• Title/Summary/Keyword: 과육색

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Sugar Content, Flesh Color and Weight of Pre-matured Fruits in Korean Prunus mume (청매실의 무게, 과육색, 당도 특성)

  • Hee Kyoung Kang;Hye Rin Kang;Jin Hyuk Kim;Ja Yeon Yi;Dong Eon Kil;Hyeon Ji Jang;Seong Yeon Kim;Hong Seon Song
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.30-30
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    • 2020
  • 본 연구는 현재 공주대학교 포장에 유전자원으로 보존하는 재래종의 매실나무 열매(청매실)에 대한 활용 가능성의 유익한 정보 및 식품학적 특성과 효능의 기초 정보를 제공하기 위하여 실시하였다. 대한민국 매실나무 집단의 열매(청매실) 무게는 평균 10.5±5.2g이었고, 범위 3.0~35.4g, 변이계수 49.5%를 나타내어 매우 다양한 무게 분포를 보였다. 청매실의 무게별 분포비율은 무게 5.1~10.0g이 50.5%로서 가장 높았고 다음으로 무게 10.1~15.0g이 27.1%, 15.1~20.0g이 10.4%, 5.0g 이하가 8.3% 순이었다. 청매실의 과육색은 백록색이 50.5%로 가장 많았으며, 다음으로 연등색이 36.5%, 등색이 12.5%, 유백색과 암등색이 각각 0.5% 순이었다. 그러나 청매실의 과육색은 황매실 성숙기 및 성숙도에 따라 과피색과 과육색이 다를 것으로 판단되었다. 청매실의 당도는 평균 6.84±0.93이었으며, 범위 4.45~8.76 °Brix로 다양하였다. 당도는 동일한 무게 집단 내에서 개체 자원 간에 다양한 변이를 나타내었으나 무게 집단별 차이를 나타내지 않았으며 변이계수도 유사한 수치이었다.

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Characterization of Phenotypic Traits and Application of Fruit Flesh Color Marker in Melon (Cucumis melo L.) Accessions (멜론 유전자원의 생육 평가와 과육색 유전형 분석)

  • Bae, Ik Hyun;Kang, Han Sol;Jeong, Woo Jin;Ryu, Jae Hwang;Lee, Oh Hum;Chung, Hee
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.478-490
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    • 2021
  • We aimed to generate basic breeding data for melon (Cucumis melo L.). A total of 219 melon accessions conserved at the National Agrobiodiversity Center (NAC) in Rural Development Administration (RDA) were used in this study, of which 72 (33%) were collected from India. The majority of accessions showed orange (42%) and white (36%) flesh color. In addition to phenotypic evaluations, the accessions were genotyped using a molecular marker for the carotenoid biosynthesis gene CmOr. DNA fragments of the expected size were amplified in 205 out of 219 accessions. Digestion of the PCR products with HinfI restriction endonuclease showed 100% concordance between phenotype and genotype in green-fleshed accessions, but 98%, 97%, and 80% concordance in orange-, white-, and creamy-fleshed accessions, respectively. Sequence analysis revealed single nucleotide changes in the three positions of SNP1, SNP2 and SNP1int in the CmOr gene among accessions. These newly found alleles suggest that there are multiple mechanisms in determining fruit flesh color in melon. Also, the phenotype data of diverse accessions obtained in this study will be a valuable source for melon breeding.

Development of SNP markers for the identification of apple flesh color based on RNA-Seq data (RNA-Seq data를 이용한 사과 과육색 판별 SNP 분자표지 개발)

  • Kim, Se Hee;Park, Seo Jun;Cho, Kang Hee;Lee, Han Chan;Lee, Jung Woo;Choi, In Myung
    • Journal of Plant Biotechnology
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    • v.44 no.4
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    • pp.372-378
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    • 2017
  • For comparison of the transcription profiles in apple (Malus domestica L.) cultivars differing in flesh color expression, two cDNA libraries were constructed. Differences in gene expression between red flesh apple cultivar, 'Redfield' and white flesh apple cultivar, 'Granny Smith' were investigated by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the red flesh apple cultivar and white flesh apple cultivar were selected for nucleotide sequence determination and homology searches. High resolution melting (HRM) technique measures temperature induced strand separation of short PCR amplicons, and is able to detect variation as small as one base difference between red flesh apple cultivars and white flesh apple cultivars. We applied high resolution melting (HRM) analysis to discover single nucleotide polymorphisms (SNP) based on the predicted SNP information derived from the apple EST database. All 103 pairs of SNPs were discriminated, and the HRM profiles of amplicons were established. Putative SNPs were screened from the apple EST contigs by HRM analysis displayed specific difference between 10 red flesh apple cultivars and 11 white flesh apple cultivars. In this study, we report an efficient method to develop SNP markers from an EST database with HRM analysis in apple. These SNP markers could be useful for apple marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in apple cultivars.

Development of SNP Molecular Marker for Red-fleshed Color Identification of Peach Genetic Resources (복숭아 유전자원의 적색 과육 판별 SNP 분자표지 개발)

  • Kim, Se Hee;Nam, Eun Young;Cho, Kang Hee;Jun, Ji Hae;Chung, Kyeong Ho
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.303-311
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    • 2019
  • Various colors of fruit skin and flesh are the most popular commercial criteria for peach classification. In order to breed new red-fleshed peach cultivar, many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection markers to screen seedlings with target traits to increase breeding efficiency. For the comparison of transcription profiles in peach cultivars differing in flesh color expression, two cDNA libraries were constructed. Differences in gene expression between red-fleshed peach cultivar, 'Josanghyeoldo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two cultivars were selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNP) were screened from peach EST contigs by high resolution melting (HRM) analysis displayed specific difference between 8 red-fleshed peach cultivars and 24 white-fleshed peach cultivars. All 72 pairs of SNPs were discriminated and the HRM profiles of amplicons were established. In the study reported here, the development of SNP markers for distinguishing between red and white fleshed peach cultivars by HRM analysis offers the opportunity to use DNA markers. This SNP marker could be useful for peach marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach cultivars.

SNP Markers Useful for the Selection of Yellow-fleshed Peach Cultivar (황육계 복숭아 품종 선발용 SNP 마커)

  • Kim, Se Hee;Kwon, Jung-hyun;Cho, Kang Hee;Shin, Il Sheob;Jun, Ji Hae;Cho, Sang-Yun
    • Korean Journal of Plant Resources
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    • v.34 no.5
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    • pp.443-450
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    • 2021
  • Peach flesh color is commercially important criteria for classification and has implications for nutritional quality. To breed new yellow-fleshed peach cultivar many cross seedlings and generations should be maintained. Therefore it is necessary to develop early selection molecular markers for screening cross seedlings and germplasm with economically important traits to increase breeding efficiency. For the comparison of transcription profiles in peach varieties with a different flesh color expression, two cDNA libraries were constructed. Differences in gene expression between yellow-fleshed peach cultivar, 'Changhowon Hwangdo' and white-fleshed peach cultivar, 'Mibaekdo' were analyzed by next-generation sequencing (NGS). Expressed sequence tag (EST) of clones from the two varieties was selected for nucleotide sequence determination and homology searches. Putative single nucleotide polymorphisms (SNPs) were screened from peach EST contigs by high resolution melting (HRM) analysis, SNP ID ppa002847m:cds and ppa002540m:cds displayed specific difference between 17 yellow-fleshed and 21 white-fleshed peach varieties. The SNP markers for distinguishing yellow and white fleshed peach varieties by HRM analysis offers the opportunity to use early selection. This SNP markers could be useful for marker assisted breeding and provide a good reference for relevant research on molecular mechanisms of color variation in peach varieties.

Coloring Quality of Prunus mume and Prunus armeniaca var. ansu - Plant Genetic Resource of ex situ Conservation in Yesan - (매실나무와 살구나무 열매의 착색 특성 - 예산지역 이식의 현지외보전 식물자원 -)

  • Kang, Hee Kyoung;Yi, Ja Yeon;Kang, Hye Rin;Oh, Chan Sik;Song, Hong Seon
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.48-48
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    • 2019
  • 본 연구는 대한민국 매실나무 (207점)와 살구나무 (7점) 열매의 특성평가를 위하여 개체별 과피 및 과육의 착색정도를 조사하였다. 매실나무 열매의 착색상태는 아주 약함이 65점 (31.4%)으로 가장 많았으며, 다음으로 약함 (57점, 27.5%), 아주 짙음 (56점, 27.1%), 중간 (19점, 9.2%), 짙음 (10점, 4.8%)이었다. 살구나무 열매의 착색상태는 아주 짙음이 3점(42.8%)으로 가장 많았고, 다음으로 아주 약함 (2점, 28.6%), 약함과 짙음 (각각 1점, 14.3%)이었다. 매실나무 열매의 착색부위는 적음이 105점 (50.7%)으로 가장 많았으며, 다음으로 중간 (65점, 31.4%), 아주 적음(32점, 15.5%), 많음 (5점, 2.4%)이었다. 살구나무 열매의 착색부위는 아주 많음이 5점 (71.4%)으로 가장 많았고, 다음으로 중간 및 많음 (각각 1점, 14.3%)이었다. 매실나무 과육의 빛깔은 백록색이 103점 (49.8%)으로 가장 많았으며, 다음으로 연등색 (77점, 37.2%), 등색 (25점, 12.0%), 유백색 및 암등색 (각각 1점, 0.5%)이었다. 살구나무 과육의 빛깔은 등색이 4점 (57.1%)으로 가장 많았고, 다음으로 연등색 (2점, 28.6%), 백록색 (1점, 14.3%)이었다.

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Ethephon mixed with Calcium Carbonate accelerate Coloration of Satuma mandarin (Citrus Unshiu Marc.) in the Plastic Film House (에스렐과 탄산칼슘에 의한 하우스 밀감의 착색 촉진)

  • 김용호;문영일
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 1998.05a
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    • pp.131-139
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    • 1998
  • 여름철 고온기를 지나는 하우스 밀감은 착색이 되기 전에 과육이 선숙되는데, 일반적으로 착색은 고온에 의해 지연된다(Goldschmidt, 1988). 소비자들은 충분히 착색되지 않고 녹색을 띤 밀감은 성숙이 안된 것으로 생각하고 있기 때문에 수확 후 에테폰 처리에 의해 착색을 촉진시키고 있으나(Jimenez-Cuesta 등, 1983) 과경부의 꼭지가 쉽게 건조되고 짙은 오렌지색으로 발색되지 않고 연한 노란 색으로 되어 상품성이 높지 못하다. (중략)

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Preparation of Stirred Yoghurt from Milk Added with Korean Loquat (Eriobotrya japonica Lindley) (국내산 비파(Eriobotrya japonica Lindley)를 첨가한 Stirred Yoghurt의 제조)

  • Go Jin-Kyoung;Park Shin-In
    • The Korean Journal of Food And Nutrition
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    • v.18 no.3
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    • pp.200-206
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    • 2005
  • This study was performed to develop a functional stirred yoghurt prepared from skim milk added with loquat(Eriobotrya japonica Lindley). Skim milk containing $15\%$ loquat extract was fermented by the mixed strains of Streptococcus thermophilus and Lactobacillus acidophilus, and then loquat flesh and oligosaccharide were added to the yoghurt base. Sensory scores of the stirred yoghurt containing $15\%$ loquat flesh and $20\%$ oligosaccharide were higher than other groups in sweet taste, sour taste and overall acceptability. When the levels of oligosaccharide of the stirred yoghurts added with $15\%$ loquat flesh were increased, the L value(brightness) and a value (redness) of the stirred yoghurt decreased, but the b value(yellowness) increased. The hardness, springiness, cohesiveness, gumminess and resilience of the $15\%$ loquat flesh stirred yoghurt added with $20\%$ oligosaccharide were higher than others.

Identification of ideal size and drivers for consumer acceptability of apple (사과의 이상적인 크기와 소비자 기호도 결정인자 분석)

  • Jung, Hee-Yeon;Kim, Sang-Sook
    • Food Science and Preservation
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    • v.21 no.5
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    • pp.618-626
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    • 2014
  • The physicochemical characteristics and consumer perceptions of two Fuji cultivars (Fuji and Royal Fuji) with six different size groups (3D: 30~39, 4D: 40~49, 5D: 50~59, 6D: 60~69, 7D: 70~79, and 8D: 80~89 apples/15 kg) were investigated to identify the ideal size and the drivers of consumer acceptability of apples. For the physicochemical characteristics, the weight, volume, specific volume, L, a, and b colors, hardness, pH, acidity, and brix of apples were measured. A total of 100 consumers were asked to mark the intensity of the characteristics (size, redness, glossiness, surface roughness, apple odor, apple flavor, sweetness, sourness, hardness, crunchiness, and toughness) to determine the ideal characteristics of apples before they were asked to taste the apple products. The consumers evaluated the apple samples in terms of their appearance, odor, flavor, texture, and overall acceptability; the consumers' intent to purchase such apples and willingness to pay for them; and the intensity of the aforementioned characteristics. Compared to the ideal characteristics of apples, the actual apple samples were rated low in their apple odor, apple flavor, acidity, sweetness, hardness, and crispness. The ideal size of the apples was between 4D and 5D. Their overall acceptability was highly affected by their flavor, followed by their texture, odor, and appearance. The acceptability of the appearance was highly correlated with the glossiness (r = 0.80), volume, weight, redness (r = 0.73), and size (r = 0.72). The consumer acceptability of the apples increased with the decreased pH and the increased Brix, hardness, and color b values of the peeled apples. The apple flavor, sweetness, hardness, crispiness, juiciness, and toughness during mastication were noted as sensory drivers of consumer acceptability.