• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.35 no.1
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• pp.1-10
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• 2022

Objectives : This study was conducted to confirm the anti-inflammatory effects of Aster glehni Water extracts. Methods : In this study, MTT assay was performed to detect cell viability. To evaluate the anti-inflammatory effects of Aster glehni Water extracts, we examined NO production in LPS-induced macrophages. Expressions of iNOS, COX-2, ERK, p38, JNK were also investigated by using western blot assay. Results : Aster glehni Water extracts have no cytotoxicity at 15.625-1,000㎍/㎖ in RAW 264.7 cells. Aster glehni Extracts inhibited the NO production in a dose-dependent manner in RAW 264.7 cells treated with LPS. Pretreated 250, 500, 1,000㎍/㎖ of Aster glehni water extracts had significantly suppressed expression levels of iNOS, COX-2, p-ERK, p-p38, p-JNK. Conclusions : These results suggest that Aster glehni Water extracts have anti-inflammatory effects and can be used for various inflammatory skin diseases.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.35 no.1
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• pp.11-23
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• 2022

Objectives : The purpose of this study is to confirm the anti-inflammatory effects of Cistanche deserticola Water Extracts(CDWE). Methods : In this study, cell viability was determined by MTT assay. NO production was determined with Griess reagent, and IL-6 production was determined by ELISA. And western blot analysis was performed to confirm the protein expression of NOS2, COX-2, I𝜅B-𝛼, p-I𝜅B-𝛼, and NF-𝜅B. Results : CDWE was not cytotoxic at 15.625-1,000㎍/㎖ in RAW 264.7 cells. CDWE inhibited NO production in a concentration-dependent manner, with inhibitory effect on expression levels of IL-6. Expression level of NOS2, NF-𝜅B p65, and p-I𝜅B-𝛼 decreased at 250-1,000㎍/㎖. mRNA expression of COX-2 suppressed at 250, 1,000㎍/㎖. Conclusions : These results suggest that CDWE has anti-inflammatory effects and can be used as an anti-inflammatory therapeutic material.

• Journal of Physiology & Pathology in Korean Medicine
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• v.36 no.2
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• pp.54-58
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• 2022

This study aimed to investigate the anti-stress function of Aucklandiae Radix (AR) and Cyperi Rhizoma (CR). The essential oils used in the experiment were extracted from AR and CR using Steam Distillation Extraction and Super critical CO₂ extraction. To observe the effects of sample administration, we measured feed intake, leukocytes, red blood cells, hemoglobin, platelets, serum serotonin content, immobility time, climbing time, and swimming time in mice subjected to chronic restraint stress as behavioral changes. The average body weight of all experimental groups increased than the average body weight of the control group. The immobility and climbing times of experimental groups A and B administered with supercritical extraction samples were shorter than those of the other experimental groups and the control group, and the swimming time was longer. The serotonin content in the blood of all experimental groups decreased compared to the normal group, and the serotonin content of the control group was increased. The authors suggest that Korean herbal medicines AR and CR may be utilized as anti-stress flavoring agents based on the above results.

• Journal of Convergence Korean Medicine
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• v.4 no.1
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• pp.29-36
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• 2022

Objectives : The purpose of this study was to investigate the anti-inflammatory effects of the aqueous extract of the steamed radix of Rehmanniae glutinosa (SRG) on periodontitis in ligature-induced rat model. Methods: To induce the periodontitis, ligature was placed around the lower first molar in rats. Rats were divided into 4 groups (n = 7), NL (non-ligatured and vehicle-treated), L (ligatured and vehicle-treated), SRG1 (ligatured and 1 mg/kg SRG-treated) and SRG100 (ligatured and 100 mg/kg SRG-treated). Vehicle or SRG solution was applied daily for 14 days and then all experimental rats were sacrificed. To examine the effect of SRG solution on periodontitis, the level of alveolar bone loss, cementum regeneration, gingival tissue degradation, and osteoclast cell numbers were analyzed. Results: Alveolar bone loss was inhibited in ligature-induced periodontitis rats treated with SRG treatment. Histopathological cementum was recovered in SRG1 and SRG100 groups. SRG extract inhibited gingival tissue degradation induced by ligature. In addition, the numbers of osteoclast cells were decreased by treatment SRG in periodontitis rats. Conclusion: Taken together, these results suggest that SRG have inhibitory effects against periodontitis. Therefore, the steamed radix of Rehmanniae glutinosa has may be a potential alternative for periodontitis.

• The Journal of the Society of Stroke on Korean Medicine
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• v.15 no.1
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• pp.13-28
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• 2014

■ Objectives The berries of Schisandra chinensis (Schisandra Fructus) are given the name Omiza in Koreane(五味子), and have been used asremedies for many ailments: to resist infections, increase skin health, and combat insomnia, coughing, and thirst. This study was designed to investigate the effects of Schisandra Fructus hexane fraction (SFH) on serum lipid levels in Hyperlipidemic mice. ■ Methods In this experiment, effects on total cholesterol, HDL-cholesterol, triglyceride, AST, ALT, fasting blood glucose in serum were measured. And in addition, histopathological and gene expression changes in liver tissue was also observed. ■ Results SFH did not affects weight gain, serum AST and ALT in hyperlipidemic mice. Oral administration of SFH lowered levels of total cholesterol and triglyceride, which were elevated by induction of hyperlipidemia. Finally, administration of SFH lowered fasting blood glucose significantly. And SFH also ameliorates anti-oxidative stress systems in internal organs which play key role in disease prevention. ■ Conclusion Results in our study suggest that SFH can prevent obese through regulation of dyslipidemia and hyperglycaemia.

• Journal of Nutrition and Health
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• v.54 no.2
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• pp.224-237
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• 2021

Purpose: Paeonia Radix Alba is a traditional herbal medicine used to treat the liver and the spleen. Many studies have reported that Paeonia Radix Alba extract (PR) affects liver injury, but there has been no study on liver injuries induced by thioacetamide (TAA). Therefore, we aimed at evaluating the effect of PR on a TAA-induced acute liver injury (ALI) model. Methods: The antioxidant activity of PR was assayed by the content of total polyphenol, total flavonoid, 1,1-diphenyl-2'-picrylhydrazyl (DPPH), and 2,2'-azino-bis (3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS) radical scavenging activities in vitro test. ALI was induced via-intraperitoneal injection of TAA (200 mg/kg body weight) for three consecutive days. Also, silymarin (100 mg/kg body weight) and PR (100 or 200 mg/kg body weight) were administered at 1 hours 30 minutes prior to TAA treatment. The levels of ammonia, glutamic oxaloacetic transaminase (GOT), and glutamic pyruvic transaminase (GPT) were analyzed using an assay kit. The expressions of antioxidant proteins including Nrf2, Keap1, HO-1, SOD, catalase, and GPx-1/2 and oxidative stress-related proteins including NOX2, p47^phox, and p22^phox were evaluated by the western blot analysis. Results: PR showed excellent antioxidant activity in vitro. TAA administration increased the levels of ammonia, GOT, and GPT in the ALI control group compared to the normal group, whereas it was significantly reduced by PR pretreatment. Moreover, NADPH oxidase protein expressions were upregulated after TAA treatment, while the elevated expressions were inhibited by PR pretreatment. The expressions of antioxidant protein were downregulated in the ALI control group, whereas Nrf2 activation in the PR group was accompanied by increased levels of antioxidant enzymes. Conclusion: PR administration increased the antioxidant enzymes via activation of the Keap1/Nrf2 pathway and inhibited the protein levels of NADPH oxidase factors. Taken together, these results showed that PR treatment may be considered to ameliorate acute liver injury induced by TAA.

• Korean Journal of Food Science and Technology
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• v.54 no.3
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• pp.288-296
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• 2022

This study aimed to verify the effect of Citrus unshiu peel water extract (CUP) on a mouse model of acute gastritis (AG) induced by HCl/ethanol. Several studies have found that CUP has anti-inflammatory effects. The AG model was induced by oral administration of 150 mM HCl/60% ethanol (550 µL) to all groups except the control group. Also, for drug treatment, sucralfate (10 mg/kg) and CUP (100 or 200 mg/kg) were orally administered for 90 minutes before induction. The effect of CUP treatment was confirmed by gross gastric mucosal damage measurement, and the levels of Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT), and myeloperoxidase were reduced as well as the levels of oxidative stress biomarkers and their related proteins. In addition, the levels of inflammatory proteins, mediators, and cytokines were significantly downregulated byPI3K/Akt signaling. Taken together, these results show that CUP treatment alleviates AG by regulating PI3K/Akt signaling.

• Proceedings of the KSRS Conference
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• 2006.03a
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• pp.49-52
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• 2006

최근 대규모 지역 혹은 전 지구에 걸친 분석 및 모니터링을 위한 위성영상의 사용이 늘어나면서 이를 처리하기 위한 효율적인 '영상좌표 상호등록'법이 요구되고 있다. 이에 본 연구에서는 일반적으로 오랜 시간이 소요되는 '영상좌표 상호등록'의 효율성을 높이기 위해 '사전검수영역기반정합법'(Pre-qualified area based matching)을 사용하였다. 이를 통해 '영상좌표 상호등록'시 연산시간을 현저히 단축시켰고 추출된 정합점에 과대오차제거법을 적용함으로서 단순히 영역기반정합법을 적용한 경우에 비해서 정확도가 향상됨을 확인할 수 있었다. 제안한 알고리즘을 이용하여 테스트 프로그램을 작성, 한반도 Landsat ETM+ 영상 3장을 이용하여 테스트하였다. 정합점 간의 평균제곱오차는 0.436 영상소, 정합점은 평균 38,475개로 나타났다. 연산시 간은 평균 약 8분으로 나타났다.

• Journal of Life Science
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• v.11 no.4
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• pp.321-327
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• 2001

The purpose of this study was to investigate the nitrite scavenging activity of the ethanol and water extracts obtained from Hizikia fusiforme and Sargassum fulvelum using sodium nitrite under different pH conditions. The nitrite scavenging activity was appeared in all the extracts examined and it showed the highest value at pH 1.2 The ethanol extract of Hizikia fusiforme provided higher activity than the water extract of that. Ohterwise, the nitrite scavenging activity of Sargassum fulvelum ethanol extract was similar to that Sargassum fulvelum water extract. There was significant difference among concentration of extracts Hizikia fusiforme and Sargassum fulvelum. Also, nitrite scavenging activity of the extracts Hizikia fusiforme and Sargassum fulvelum decreased with higher pH condition. In addition, the activity was increaed with higher concentration of extracts Hizikia fusiforme and Sargassum fulvelum in the course of reaction time 30 minute to 3 hour.

• Journal of Korean Medicine for Obesity Research
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• v.20 no.2
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• pp.69-77
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• 2020

Objectives: In this study, we investigated the antiobesity and antidiabetic effects of polyherbal extract, DM2 consisting of Atractylodis Rhizoma, Anemarrhenae Rhizoma, Cinnamomi Cortex, and Moutan Radicles Cortex in high fat diet-induced obesity mice. Methods: DM2 extract was prepared with a hot water. Six-week-old male C57BL/6N mice were fed a high-fat diet (HFD) for 8 weeks and then administrated with DM2 extract (500 mg/kg, p.o.) for 4 weeks. The changes of physiological markers, body weight (BW), food and water intakes, and the levels of fasting blood glucose (FBG) were measured once a week for 4 weeks in mice. The the serum levels of glucose, insulin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (T-CHO), triglyceride, and low density lipoprotein cholesterol in sera were measured in mice using autometic chemical analyzer and enzyme linked immunosorbant assay. We also observed the histological changes of liver and pancreatic tissues with Hematoxylin & Eosin staining. Results: In physiological change, the increases of BW, calorie intake, and FBG in HFD-induced obese mice were significantly decreased after administration of DM2 extract for 4 weeks. The decrease of water intake was significantly increased in DM2 extract-administrated mice. In serological change, the administration of DM2 extract in obesity mice was significantly decreased the serum levels of glucose, insulin, T-CHO, AST, and ALT levels. We also found that DM2 extract inhibited the increase of lipid droplets in liver and the structural destruction of pancreatic tissues in obesity mice. Conclusion: Our study demonstrated that DM2 extract has antiobesity antidiabetic effects with body weight loss, decrease of glucose and insulin levels, and lipid accumulation on liver tissue.