Browse > Article
http://dx.doi.org/10.6114/jkood.2022.35.1.001

Anti-inflammatory Effects of Aster glehni Water Extracts in LPS-stimulated RAW 264.7 Macrophages  

Ko, Ho-Geon (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Lee, Kyou-Young (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Hong, Chul-Hee (Department of Ophthalmology, Otolaryngology & Dermatology, College of Korean medicine, Sangji University)
Publication Information
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology / v.35, no.1, 2022 , pp. 1-10 More about this Journal
Abstract
Objectives : This study was conducted to confirm the anti-inflammatory effects of Aster glehni Water extracts. Methods : In this study, MTT assay was performed to detect cell viability. To evaluate the anti-inflammatory effects of Aster glehni Water extracts, we examined NO production in LPS-induced macrophages. Expressions of iNOS, COX-2, ERK, p38, JNK were also investigated by using western blot assay. Results : Aster glehni Water extracts have no cytotoxicity at 15.625-1,000㎍/㎖ in RAW 264.7 cells. Aster glehni Extracts inhibited the NO production in a dose-dependent manner in RAW 264.7 cells treated with LPS. Pretreated 250, 500, 1,000㎍/㎖ of Aster glehni water extracts had significantly suppressed expression levels of iNOS, COX-2, p-ERK, p-p38, p-JNK. Conclusions : These results suggest that Aster glehni Water extracts have anti-inflammatory effects and can be used for various inflammatory skin diseases.
Keywords
Aster glehni; Anti-inflammation; iNOS; COX-2; MAPK;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Cho W, Nam JW, Kang HJ, Windono T, Seo EK, Lee KT. Zedoarondiol isolated from the rhizoma of Curcuma heyneana is involved in the in hibition of iNOS, COX-2 and pro-inflammatory cytokines via the downregulation of NF-κ B pathway in LPS-stimulated murine macrophages. Int. Immunopharmacol. 2009;9(9):1049-57.   DOI
2 Kim DH, Hwang EY, Son JH. Anti-inflammatory activity of Carthamus tinctorious seed extract in Raw 264.7 cells. J Life Sci. 2013;23(1):55-62.   DOI
3 Martel-Pelletier J, Pelletier JP, Fahmi H. Cyclooxygen-ase-2 and prostaglandins in articular tissues. Semin Arthritis Rheum. 2003;33(3):155-67.   DOI
4 Yoon JH, Youn K, Ho CT, Karwe MV, Jeong WS, Jun M. p-coumaric acid and ursolic acid from Corni fructus attenuated β-amyloid(25-35)-induced toxicity through regulation of the NF-κB signaling pathway in PC12 cells. J Agric Food Chem. 2014;62(21):4911-6.   DOI
5 Lee HM, Yang GS, Ahn TG, Kim MD, An HJ. Antiadipogenic Effects of Aster glehni Extract: In Vivo and In Vitro Effects. Evid Based C omplement Alternat Med. 2013;2013:1-10.
6 Hwang JH, Kim KJ, Ryu SJ, Lee BY. Caffeine prevents LPS-induced inflammatory responses in RAW 264.7 cells and zebrafish. Chemico biological interaction. 2016;248:1-7.   DOI
7 Kao SJ, Lei HC, Kuo CT, Chang MS, Chen BC, Chang YC, et al. Lipoteichoic acid induces nuclear factor-kappaB activation and nitric oxide synthase expression via phosphatidy linositol 3-kinase, Akt, and p38 MAPK in RAW 264.7 macrophages. Immunology. 2005;115(3):366-74.   DOI
8 Jeong H, Sung M, Kim Y, Ham H, Choi Y, Lee J. Anti-inflammatory activity of Salvia plebeia R. Br. leaf through heme oxygenase-1 inducti on in LPS-stimulated RAW 264.7 macrophages. J Korean Soc Food Sci Nutr. 2012;41(7):888-94.   DOI
9 Kim DH, Hwang EY, Son JH. Anti-inflammatory activity of Carthamus tinctorious seed extracts in Raw 264.7 cells. J Life Sci. 2013;23(1):55-62.   DOI
10 Sung YY, Kim DS, Yang WK, Nho KJ, Seo H S, Kim YS, et al. Inhibitory effects of Drynaria fortunei extracts on house dust mite antigen-induced atopic dermatitis in NC/Nga mice. J Ethnopharmacol. 2012;144(1):94-100.   DOI
11 Guzik T, Adamek-Guzik T. Nitric oxide and superoxide in inflammation. J Physiol Pharmacol. 2003;54(4):469-87.
12 Jeong DH, Kim KBWR, Kang BK, Jung SA, Kim HJ, Jeong HY, et al. Anti-inflammatory activity of the Undaria pinnatifida water extract. J Appl Biol Chem. 2012;55(4):221-5.   DOI
13 Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Kim BR, et al. Anti-inflammatory activity of an ethanol extract of Laminaria japonica root on lipopolysaccharide-induced inflammatory response in RAW 264.7 cells. Korean J Food Sci Technol. 2014;46(6):729-33.   DOI
14 Seo SW, Kim KS. Antioxidant Activities of Aster glehni Extracted with Different Solvents. Iran J Public Health. 2019;48(1):176-8.
15 Simmons ML, Murphy S. Induction of nitric oxide from glial cells. J Neurochem. 1992;59(3):8 97-905.   DOI
16 Lee YS, Kim HS, Kim SK, Kim SD. IL-6 mRNA Expression in Mouse Peritoneal Macrophages and NIH3T3 Fibroblasts in Response to Candida albicans. J Microbiol Biotechnol. 2000;10(1):8-15.
17 Nugroho A, Kim MH, Choi JW, Choi JS, Jung WT. Phytochemical studies of the phenolic substances in Aster glehni extract and its sedative and anticonvulsant activity. Arch Pharm Res. 2012;35(3):423-30.   DOI
18 Kundu JK, Surh YJ. Inflammation: gearing the journey to cancer. Mutat Res. 2008;659(1-2):15-30.   DOI
19 Arthur JS, Ley SC. Mitogen-activated protein kinases in innate immunity. Nat Rev Immunol. 2013;13(9):679-92.   DOI
20 Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ, Jeong JH. Anti-inflammatory effects of extracts f rom Ligustrum ovalifolium H. leaves on RAW264.7 macrophages. J Korean Soc Food Sci Nutr. 2012;41(9):1205-10.   DOI
21 Miyasaka N, Hirata Y. Nitric oxide and inflammatory arthritides. Life Sci. 1997; 61(21):2073-81.   DOI
22 Needleman P, Isakson PC. The discovery and function of COX-2. J Rheumatol. 1997;49:6-8.
23 Seybold VS, Jia YP, Abrahams LG. Cyclo-oxygenase-2 contributes to central sensitization in rats with peripheral inflammation. Pain. 2003;105(1-2):47-55.   DOI
24 Kim HH, Park GH, Park KS, Lee JY, An BJ. Anti-oxidant and Anti-inflammation Activity of Fractions from Aster glehni Fr. Schm.. Kor. J. Microbiol. Biotechnol. 2010;38(4):434-41.
25 Nathan C. Points of control in inflammation. Nature. 2002;420(6917):846-52.   DOI
26 Jung BS, Shin MG. Dohae Hyangyak Dictionary. Yeonglimsa. 2003:1020-1.
27 Meyer CF, Wang X, Chang C, Templeton D, Tan TH. Interaction between c-Rel and the mitogen-activated protein kinase kinase kinase 1 signaling cascade in mediating kappaB enhancer activation. J Biol Chem. 1996;271(15):8971-6.   DOI
28 Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990;144(4):1425-31.   DOI