• Food Science and Preservation
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• v.20 no.5
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• pp.727-734
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• 2013

The antifungal activity against cheese fungi by lactic acid bacteria isolated from kimchi was investigated. Eight fungi were isolated from cheese in the cheese ripening room. Two of them were identified as Penicillium and Cladosporium via ITS-5.8S rDNA analysis. Twenty-two species of lactic acid bacteria with antifungal activity were isolated from kimchi. Two of them were identified as Lactobacillus and Pediococcus via 16S rRNA sequence analysis. Of the 22 lactic acid bacteria species, six were selected (L. sakei subsp. ALJ011, L. sakei subsp. ALI033, L. sakei subsp. ALGy039, P. pentosaceus ALJ015, P. pentosaceus ALJ024 and P. pentosaceus ALJ026) due to their higher activity against the eight fungi isolated from cheese in the cheese ripening room; and among the six species, the P. pentosaceus ALJ015 and P. pentosaceus ALJ024 isolates from the Jeonju area kimchi and the L. sakei subsp. ALI033 isolate from the Iimsil area kimchi had higher antifungal activity than the other lactic acid bacteria. The minimum inhibitory concentration (MIC) of L. sakei subsp. ALI033 against the eight fungi isolated from cheese in the cheese ripening room was $62.5{\mu}g/mL$.

• Korean Journal of Organic Agriculture
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• v.14 no.4
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• pp.399-410
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• 2006

This study was carried out to identity the effects of antifungal bacteria isolated from the soil grown kiwi fruit plants on the growth inhibition of Botrytis cinerea causing gray mold in kiwi fruit plants in the southern districts of Jeonnam. Two hundred and fifty antagonistic microorganisms were isolated and examined into the antifungal activity against Botrytis cinerea. We screened and isolated four bacterial strains which strongly inhibited Botrytis cinerea from the soil grown kiwi fruit plants. And the best antifungal bacterial strain which called CHO 163 was finally selected. Antagonistic microorganism CHO 163 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. CHO 163 showed 86.9% of antifungal activity against Botrytis cinerea. By the bacterialization of culture broth and heated filtrates of culture broth, Bacillus sp. CHO 163 showed almost all of antagonistic activity against Botrytis cinerea. And we also confirmed that in vitro the treatment of Bacillus sp. CHO 163 cultured by SD+B+P broth efficiently controled the growth of Botrytis cinerea causing gray mold in kiwi fruit plants.

• Journal of Life Science
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• v.30 no.12
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• pp.1128-1139
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• 2020

Over recent years, it has become evident that food and agricultural products are easily contaminated by fungi of Aspergillus, Fusarium, and Penicillium due to rapid climate change, which is not only a global food quality concern but also a serious health concern. Owing to consumers' interest in health, resistance to preservatives such as propionic acid and sorbic acid (which have been used in the past) is increasing, so it is necessary to develop a substitute from natural materials. In this review, the role of lactic acid bacteria as a biological method for controlling the growth and toxin production of fungi was examined. According to recent studies, lactic acid bacteria effectively inhibit the growth of fungi through various metabolites such as organic acids with low molecular weight, reuterin, proteinaceous compounds, hydroxy fatty acids, and phenol compounds. Lactic acid bacteria effectively reduced mycotoxin production by fungi via adsorption of mycotoxin with lactic acid bacteria cell surface components, degradation of fungal mycotoxin, and inhibition of mycotoxin production. Lactic acid bacteria could be regarded as a potential anti-fungal and anti-mycotoxigenic material in the prevention of fungal contamination of food and agricultural products because lactic acid bacteria produce various kinds of potent metabolic compounds with anti-fungal activities.

• KOREAN POULTRY JOURNAL
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• v.7 no.6 s.68
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• pp.89-91
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• 1975

• Journal of Dairy Science and Biotechnology
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• v.31 no.2
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• pp.133-141
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• 2013

This study was performed to identify the cheese starter potential of antifungal lactic acid bacteria isolated from Kimchi. Eight fungi were isolated from cheese or the cheese ripening room, and identified as Penicillium and Cladosporium by ITS-5.8S rDNA analysis. Twenty-two lactic acid bacteria species with antifungal activity were isolated from Kimchi, and identified as Lactobacillus and Pediococcus by 16S rRNA sequence analysis. Six lactic acid bacteria species were selected (L. sakei subsp. ALJ011, L. sakei subsp. ALI033, L. sakei subsp. ALGy039, P. pentosaceus ALJ015, P. pentosaceus ALJ024, and P. pentosaceus ALJ026) based on higher antifungal activity from the initial 22 species. Out of the six identified species, L. sakei subsp. ALI033 had the highest antifungal activity. For growth of the six lactic acid bacteria, optimal temperature and pH were $30{\sim}37^{\circ}C$ and 7.0, respectively. Proteolytic activities of the six lactic acid bacteria were almost as strong as the commercial strain Str. thermophilus Body-1. Coagulative activities of L. sakei subsp. ALI033, P. pentosaceus ALJ015, and P. pentosaceus ALJ024 were higher than those of L. sakei subsp. ALJ011, L. sakei subsp. ALGy039, and P. pentosaceus ALJ026. The acid resistance of L. sakei subsp. was higher than that of P. pentosaceus. The major organic acid component of the lactic acid bacteria culture medium was lactic acid.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2012.04a
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• pp.448-451
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• 2012

현대의 사회는 화재 등과 같은 많은 위험요소를 포함하고 있으며 이중 곰팡이와 같은 미생물에 의해 인체에 미치는 피해 또한 무시할 수 없다. 곰팡이는 우리의 주변에서 흔히 관찰 할 수 있는데 건물 실내의 벽면에서 주로 확인 할 수 있으며 곰팡이 균의 독소를 조사하여 보면 크게 세 가지로 분류 할 수 있다. 곰팡이 독소는 곰팡이 속들에 의해 생성되는 것으로서 아플라 톡신이 대표적인 예이며, 그 외에도 오크라톡신, 파튤린 등이 있고 곰팡이 독소는 곰팡이가 생산하는 2차 대사산물로 발암 물질을 포함하고 있어 인체에 큰 위험 요소라 할 수 있는데 본 연구에서는 건축물의 벽면에 발생하는 곰팡이 균을 편백수(피톤치드)를 활용한 방재 기술을 연구하고자 한다.

• Journal of Food Hygiene and Safety
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• v.2 no.3
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• pp.97-102
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• 1987

The effects of gamma irradiation on microbiological and physicochemical qualities in raw ingredients (thirteen kinds of cereal grain and their by-product) of mixed feeds were investigated. The total aerobic bacteria counts in the samples were $10^2\;to\;10^6/g$. They were sterlllzed to a undetectable level by 5 to 7 kGy irradiation. Coliforms were contaminated in high levels in all sample, ranging from $10^2\;to\;10^6/g$. They were radiation-llensitive and completely eliminated by irradiation with 3 to 5 kGy. Total fungi, ranging from $10^2\;to\;10^4/g$, mainly osmophiles were identified as Aspergillus and Penicillium. They were eliminated below identification limit by 5 to 7 kGy irradiation. Seven kinds of species, including Aspergillus IkrlJUB. were identified as a potential mycotoxin producers. Physicochemical qualities, such as total amino acid content, total sugar content. TBA value and color difference showed that an optimum dose of irradiation was less detrimental than ethylene oxide fumigation.

• Product Safety
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• s.209
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• pp.15-15
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• 2011

보령메디앙스(주)가 소비자의 안전확보를 위해 "물휴지(물티슈)" 중 일부 제품에 대한 자발적 리콜을 시행한다고 밝혔다.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.1
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• pp.117-123
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• 2022

Recently, due to COV ID-19, many people are spending most of their time indoors. So, there is a rising interest on the indoor air quality in the field of building construction. The main sources for the indoor air pollution are human indoors activity, building materials, living supplies and the polluted air from outdoor. The Korean government has designated 17 indoor air pollutants including fine dust, total airborne bacteria, fungi and carbon dioxide, etc.. Most people are always exposed to assorted bacteria and molds in our daily life, because indoor environment for human, moderate temperature are humidity, it is favourable to the growth of most of bacteria and fungi. Pine needles have an antibacterial effect against bacteria and fungi. In this study, the antibacterial activity against bacteria and fungi was tested by cement matrix using pine needle extract. As a result, the cement matrix using pine needle extract showed antibacterial activities against bacteria, but in the case of fungi, it did not show antifungal activity.

• The Korean Journal of Mycology
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• v.42 no.2
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• pp.138-143
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• 2014

Several isolates were collected from apple, pepper, strawberry, cucumber and tomato having typical gray mold symptoms. All the isolates were identified as Botrytis cinerea by using morphological characteristics and PCR-RFLP method. It was difficult to analyze the phylogenetic relationship of these isolates by using ITS region, HSP60 and G3PDH because these genes were highly homologous in their nucleotide in inter-species of B. cinerea and intra-species of genus Botrytis. However, phylogenetic analysis using combined sequences (RPB2, HSP60 and G3PDH genes) clearly showed that all isolate of B. cinerea were different from Botrytis spp. Furthermore, it was also confirmed that strawberry isolate was distantly related to apple, pepper, cucumber and tomato isolates that were closely related to each other in nucleotide level.