• Korean journal of applied entomology
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• v.60 no.2
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• pp.201-202
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• 2021

We confirmed the convenient bioassay method for the black cutworm, Agrotis ipsilon in semi-field conditions. And we surveyed the susceptibility to commercial pesticides of A. ipsilon, which feeds Chinese cabbage through this bioassay process. As a result of the test, six soil mixing insecticides and eight soil drenching insecticides showed more than 90% insecticidal activity.

• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.206-211
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• 2021

Hygienic behavior of Honey bees, Apis mellifera, was evaluated by uncapping and removing ability of dead broods from the nest. Hygienic behavior is originated from quantitative traits, which are expected to express key roles in colony defense against mite parasites and bacterial and fungal diseases. It is regarded as one of important characteristics of honey bee's resistance to parasites and pathogens. In this study, five inbreed and two hybrid lines of A. mellifera, the former five inbreed lines, which have been reared for over eight years at the National Academy of Agricultural Science in Korea, and the latter two hybrid lines, which have been bred by crossing between the inbreed lines, were investigated on their hygienic behavior by a pin-killed brood assay at 12hrs and 24hrs after treatment. The results indicated that after 12hrs one inbred line was proved to be hygienic (removal rate of dead brood >90%), three inbred and two hybrid lines showed intermediate behavior, and one inbred line belonged to non-hygienic (removal rate of dead brood <70%). However, after 24hrs, only one line was considered to be intermediate as removal rate was below 90%, thus all except this line had shown hygienic behavior.

• Communications of Mathematical Education
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• v.18 no.1 s.18
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• pp.257-266
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• 2004

자연의 세계에서 나뭇잎, 돌기물, 구름, 해안선, 곤충의 모습 등에 내재하고 있는 아름다움은 흔히 균형성, 대칭성, 다양성 등으로부터 비롯된다. 자연 현상은 복소수를 활용하여 극좌표 표현으로 묘사되는 경우가 많다. 본 논문에서는 1989년 Temple H. Fay가 Amer. Math. Monthly 96(5)호에서 발표한 나비곡선 r= e$^{cos{\theta}}$-2cos4${\theta}$+sin$^5$($\frac{\theta}{12}$)의 기하학적 성질을 대칭 이동, 회전 이동, 수치적분, 미분, 극좌표계, 삼각함수, 지수함수 및 매개함수의 표현 등 고등학교 및 대학의 미적분학 관점에서 살펴 보고 극좌표 도형에 관한 흥미 유발과 더불어 컴퓨터 활용 방법을 제시하기로 한다. 수학전문 소프트웨어인 매스매티카를 활용하여 나비곡선의 작도 및 기하학적 성질을 분석하고자 한다.

• Korean journal of applied entomology
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• v.56 no.3
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• pp.301-308
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• 2017

Locusts, Locusta migratoria (Orthoptera: Acrididae) are periodical unpredictable agricultural pests worldwide and cause serious damage to crop production; however, little consideration has been given to the management of this pest. Herein, we constructed a locust-pathogenic fungal library and confirmed that some fungi could be used as resources for locust management. First, the entomopathogenic fungi were collected from sampled soils using a Tenebrio molitor-based baiting system. For the locust assay, a locust colony was obtained from the National Institute of Agricultural Science and Technology. A total of 34 entomopathogenic fungal granules, which were produced by solid cultures, were placed in the plastic insect-rearing boxes (2 g/box) and nymphs of locust were contained in the box. In 3-7 days, mycosis was observed on the membranous cuticles of the head, abdomen, and legs of locusts. In particular, Metarhizium anisopliae, M. lepidiotae, and Clonostachys rogersoniana exhibited high virulence against the locust. Given that the 34 isolates could be used in field applications, their conidial production and stability (thermotolerance) were further characterized. In the thermotolerance assay, Paecilomyces and Purpureocillium isolates had higher thermotolerance than the other isolates. Most of the fungal isolates produced ca. >$1{\times}10^8conidia/g$ on millet grain medium. In a greenhouse trial, the granular application of M. anisopliae isolate on the soil surface resulted in 85.7% control efficacy. This work suggests that entomopathogenic fungi in a granular form can be effectively used to control the migratory locust.

• Korean journal of applied entomology
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• v.55 no.3
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• pp.257-266
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• 2016

Subtropical insect pests expand their habitats by migration to temperate zones along with global climate change. A subtropical insect pest, Maruca vitrata, is infesting leguminous crops including azuka beans in Korea and gives significant economic damages. Its great genetic variation raised an issue of the origin of a Korean M. vitrata population. To understand the genetic character of the Korean population, its cytochrome oxidase subunit 1 (cox 1) gene was sequenced and phylogenetically analyzed with other regional populations. The world populations of M. vitrata were grouped into three clusters: Asia-African, American, and Oceanian. The Korean population was classified into Asia-African cluster. To characterize the insecticide susceptibility of the Korean population, seven different insecticides (4 neutoxic insecticides, 1 insect growth regulator, 2 biopesticides) were assessed. Young larvae of M. vitrata were relatively susceptible to all tested insecticides. However, old larvae were much less susceptible than young larvae. No test insecticides effectively (> 50%) killed the old larvae of M. vitrata within 7 days.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.523-528
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• 2016

The migratory locust Locusta migratoria, one of the world's most notorious insect pests, has polyphenic (gregarious or solitarious) characteristics. Although this species is known to have several morphological variants, it is genetically divided into two different lineages using mitochondrial genome analysis: Southern (Africa, Southern Europe, Southern Asia, and Australia) and Northern (East Asia and the Eurasian continent). In 2014, a large number of orange black-colored gregarious L. migratoria nymphs suddenly appeared at Haenamgun, Jeollanamdo in the south of Korea. This is the first report of gregarious phase locusts occurring in Korea. In this study, mitochondrial COI sequences of one nymph and 11 adults of L. migratoria were analyzed to examine the genetic lineage of the gregarious nymphs of L. migratoria. Our results showed that all 12 individuals belong to the Northern linage and have low intraspecific genetic divergences (0.0% - 0.9%).

• Journal of Life Science
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• v.27 no.2
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• pp.247-266
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• 2017

Cordyceps is a traditional Chinese medicinal herb well-known in China, Korea and Japan since B.C. 2,000. The original entomopathogenic fungus, Cordyceps sinensis belonging to the genus Cordyceps could not be found inside Korean peninsula due to the absence of the host insect for the corresponding entomogenous fungus. The development of artificial production methods of Korean type Cordyceps using the silkworm Bombyx mori as in vivo culture medium for the the entomopathogenic fungus Paecilomyces tenuipes is the first, and wonderful occasion in the research history of insect industry of this global world. The aim of this article is to review the historical research background, mass-production methods, and pharmacological effects of the silkworm-dongchunghacho (Paecilomyces tenuipes) which is a newly developed Korean medicinal insect-borne mushroom, and another non-insect-borne medicinal mushroom (Cordyceps militaris and Cordyceps pruinosa). Their biological actions include anti-tumor, immunostimulating, anti-fatigue, anti-stress, anti-oxidant, anti-aging, anti-diabetic, anti-inflammatory, anti-thrombosis, hypolipidaemic and insecticidal effects. The bioactive principles are protein-bound polysaccharides (hexose, hexosamin), cordycepin, D-manitol, acidic polysaccharide etc. Protein-bound polysaccharides and n-butanol fractions were demonstrated to show a significant anti-tumor activities but did not show a cytotoxicities. D-mannitol exhibited a significant prolongation of the life span in tumor bearing mice. Ergosterol did not show an efficient anti-tumor activity, but showed a significant phagocytosis enhancing activity. Anti-tumor activity of silkworm-dongchunghacho might be attributed to immuno-stimulating activities rather than cytotoxic effects [164]. Also this review comprises the breeding of Dongchunghacho varieties, optimization of culture conditions, improvement of learning and memory by Dongchunghacho, application of them as foods and chemical constituents.

• Korean journal of applied entomology
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• v.11 no.2
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• pp.91-99
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• 1972

지리산은 해발 1915m인 남한 본토 최고인 명산이며 국립공원으로 지정된 바도 있으나 아직 그 동물상에 대한 조사는 불충분한 바 있다. 1972년 8월 11일-14일의 4일간에 걸쳐 남궁 윤은 한라산 거미상과의 비교 검토를 위하여 지리산의 제 2 고봉인 반야봉과 제 3고봉인 노고단을 중심으로 한 지리산 서남면인 "겉지리산"의 일부를 조사하였다. 한편 백외는 1971년 7일 하순 화엄사, 노고단 및 피아골의 거미상에 대해 조사한 바 있다. 광대한 지리산의 전체적인 면모를 파악하기에는 미급한 조사였지만 대치적인 특징을 추찰한 수 있었기에 이에 보고코져 한다. 지리산 거미에 대하여는 백 갑용(1942-1972), 백운하외 (1970) 등에 의해 18과 76종 (3미확정종포함)이 보고된 바 있으나 채집기록에 불분명한 바가 있고, 그 환경이나 수직분포 등에 대하여는 언급된 바가 없었다. 금번 조사한 장소를 고도별로 보면 다음과 같다. 연곡사(300-400m), 화엄사(400-600m), 피아골(500-900m), 화엄사골(600-900m), 용수바위 (900-1300m), 코재 (900-1300m), 임걸령(1400-1500m), 노고단(1400-1550m), 반야봉(1550-1750m) (1) 이 조사보고에서는 종전의 보고에서 기록이 분명한 것을 종합하여 21과 135종 (미확정종 6종 포함)의 목록을 작성하였다. (2) 지리산 추가종 56종을 확인하였으며 그 중에서 한국 미기록종은 16종이다. (목록에 *표를 한 것) (3) 미확정종은 개체특징이 분명한 것만 수록했으며 이들 중 금후 연구로써 신종으로 기재됨 것도 있다. (4) 이번 조사의 결과로는 지리산의 거미상은 북방종 26종$(20.2\%)$ 남방종 8종$(6.2\%)$ 세계공통종이 3종$(2.3\%)$으로 북방온대성 $(71.3\%)$의 경향이 짙으다. (5) 고도분포는 반야봉 (1550-1750m)에서 18종$(7.3\%)$, 1400-1550m가 38종$(15.5\%)$, 900-1400m에서 39종$(15.8\%)$, 600-900m에서 72종$(29.2\%)$, 600m이하에서 79종$(32.1\%)$으로 고도가 높아짐에 따라 종수가 점감되고 1300m 이상에 서식하는 것은 44종$(32.6\%)$에 불과하였다. (6) 지리산의 우점종은 대륙접시거미 (Prolinyphia emphana)로 600m 이상부터 산정부에 걸쳐 널리 분포하며, 개체수에 있어서도 가장 많았다. 깡충거미과(Salticidae), 늑대거미과(Lycosidae)가 극히 적은 것도 특징적이라 하겠다.

• Korean journal of applied entomology
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• v.57 no.4
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• pp.323-328
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• 2018

Despite rising global concerns over the potential impacts of invasive alien species on indigenous species, the consequences of such introductions are lacking. The cabbage seedpod weevil (CSPW, Ceutorhynchus obstrictus) was found invaded into Korea in the early of 1990s. A large-scale survey in Korea reveals that, in about 20 years post-arrival, the invasive CSPW has become the most abundant on rape flowers and spread almost parts of South Korea including Is. Jeju and Is. Ulleung. Its spread is concurrent with the habitat retraction to cruciferous weeds of the native rapseed weevil (RSW, C. albosuturalis). However, Is. Yeoseo located between the mainland and Is. Jeju, showed their ratio with about 1:1. It suggests that the population of CSPW recently entered this island. Although underlying mechanisms of the decline of native species and increase of invaded species remain unknown, the potential roles of exploitative competition cannot be ruled out. Given that invasive weevil can rapidly replace native congeners, increased level of surveillances is demanding to discourage further invasion of othe alien pests.

• Journal of Sericultural and Entomological Science
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• v.53 no.2
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• pp.87-91
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• 2015

There is no specific data on Entomogenous fungus (DongChungHaCho) in accordance with the silkworm varieties, despite of very high value industrial use as functional materials at domestic and abroad. In this study, we investigated culture condition and characteristics of Paecilomyces tenuipes in 3 silkworm cultivars and 2 specific silkworm breeds. Infection rate of P. tenuipes for silkworm varieties was the highest Kumokjam, followed by Baegokjam, Daeseongjam, Golden silk, Yeonnokjam in that order. The optimum culture conditions were as follows: dark condition, $22^{\circ}C{\pm}1$, and about $4cm^2$ in planting density per pupa. Also, we have developed annual production technologies of P. tenuipes. First, in order that the moisture content of the infection pupa became 3% or less in the natural condition. Second, the dried pupa must be vacuum-packed and was kept under $4^{\circ}C$. Finally, by restoring the moisture content of the dried pupa and culture in optimal condition, the technique for annual production of the P. tenuipes was achieved. Therefore, we expect that the use of Kumokjam and annual production technique will contribute significantly to farm income.